38 results about "Link protein" patented technology

A process for the manufacture of natural fiber and polymer composites is described. Thermoplastically processed plasticized soy flour based plastics are used with thermoplastic polymers. Polymers of soy flour and an in situ polymerized polyvinyl polymer which links proteins and carbohydrates in the flour to form the polymer are used. The composites are useful in engineering materials.

Isolated polypeptides or polypeptide chains are modified by di-tyrosine cross-linking such that the retain at least one functional activity. In one embodiment, the isolated polypeptide or polypeptide chains comprise at least one di-tyrosine cross-link, wherein at least one tyrosine of the di-tyrosine cross-link originates from a point mutation to tyrosine, and wherein the di-tyrosine cross-linked protein retains at least one function displayed by the protein in the absence of di-tyrosine cross-linking. In another embodiment, the di-tyrosine cross-linked polypeptide or polypeptide chain has enhanced stability compared to the same polypeptide or polypeptide chain in the absence of di-tyrosine cross-linking. A method for stabilization of a polypeptide or polypeptide complex, by the introduction of intra-polypeptide and / or inter-polypeptide di-tyrosine bonds, which simultaneously maintains the structure and function of the polypeptide or polypeptide complex is also described.

A process is provided for modifying growth or architecture of plants by altering the level or the functional level of a cell division controlling protein, preferably a cell-division controlling protein that binds phosphorylates retinoblasoma-link proteins, more preferably a cyclin, particularly a D-type cyclin within cells of a plant. Also provided are chimeric genes comprising a transcribed DNA region encoding an RNA or a protein, which when expressed either increases or decreases the level or functional level of a cell-division controlling protein, and plant cells and plants expressing such chimeric genes.

The present invention demonstrates a method for inhibiting O-linked protein glycosylation in a tissue or cell, comprising the step of contacting said tissue or cell with (Z)-1-[N-(3-Ammoniopropyl)-N-(n-propy- l)amino] diazen-ium-1,2-diolate or a derivative thereof. The present invention is also directed to a method of treating or inhibiting the onset of diabetes mellitis in an individual in need of such treatment, comprising the step of admininstering to said individual a pharmacological dose of a compound which inhibiting O-linked protein glycosylation in a tissue or cell of said individual. Further, the present invention provides a pharmaceutical composition, comprising (Z)-1-[N-(3-Ammoniopropyl)-N-(n-propyl)amino] diazen-ium-1,2-diolate and a pharmaceutically acceptable carrier or a derivative thereof.

The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

The present invention provides fusion proteins comprising a first molecule, and a second molecule which is a monovalent immunoglobulin or a fragment of a monovalent immunoglobulin with a long half-life when administered in vivo, methods of making such fusion proteins, pharmaceutical compositions comprising such fusion proteins, and uses thereof.

The invention discloses a double-functional fusion protein based on an antibacterial peptide, a preparation method and an application thereof. The double-functional fusion protein based on the antibacterial peptide is a fusion protein based on a cecropins B protein sequence and a human epidermal growth factor; the linking protein is especially designed to be a locus which can be identified by thrombin; and a purified His tag is constructed on the fusion protein. In the invention, the biological preparation for the fusion protein is realized by using a prokaryotic expression system and the purified fusion protein is obtained. A bacteriostasis test and a verification for realizing live animals are performed by the purified fusion protein to indicate that the fusion protein can be applied to preparing a medicine for treating epidermal wound, can be applied to diseases such as epidermal wound, abrade, burn and scald, etc., and can prevent wound from being infected.

Methods and reagents for site-selective functionalization of peptides and proteins. The methods most generally involve the reaction of a thioester with hydrazine. Reagents include bifunctional reagents of formula:H2N—NH—CH2-M-L-FGand salts thereof where M is a single bond or a chemical group carrying a non-bonding electron pair, such as —C(O)NR′—, where R′ is H, or an alkyl or aryl group; L is an optional linker group as described above; and FG is a functional group having reactivity that is orthongonal to that of the hydrazine group. FG can, among others, be an azide, alkenyl, alkynyl, nitrile (—CN) or triazole group and is preferably an azide group (—N3). Methods and reagents can, for example, be combined with intein-mediated protein splicing to link proteins or fragments thereof to various chemical species or to a surface. Surface immobilization of proteins via the methods herein results in immobilized proteins which substantially retain biological activity and is thus useful for the generation of peptide or protein microarrays. Kits for functionalization and / or immobilization of peptides and proteins are provided as well as microarrays of peptides, proteins or both.

Methods and reagents for site-selective functionalization of peptides and proteins. The methods most generally involve the reaction of a thioester with hydrazine. Reagents include bifunctional reagents of formula:H2N—NH—CH2-M-L-FGand salts thereof where M is a single bond or a chemical group carrying a non-bonding electron pair, such as —C(O)NR′—, where R′ is H, or an alkyl or aryl group; L is an optional linker group as described above; and FG is a functional group having reactivity that is orthongonal to that of the hydrazine group. FG can, among others, be an azide, alkenyl, alkynyl, nitrile (—CN) or triazole group and is preferably an azide group (—N3). Methods and reagents can, for example, be combined with intein-mediated protein splicing to link proteins or fragments thereof to various chemical species or to a surface. Surface immobilization of proteins via the methods herein results in immobilized proteins which substantially retain biological activity and is thus useful for the generation of peptide or protein microarrays. Kits for functionalization and / or immobilization of peptides and proteins are provided as well as microarrays of peptides, proteins or both.

The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.

The invention relates to a method for testing gap linking protein 26 gene GJB2 mutation related to hereditary hearing impairment, which completely enlarges GJB2 gene base boot sector, exon 1, exon 2 and shear zone by polymerase chain reaction; and then, DNA sequence is measured to detect whether GJB2 genetic mutation exists; the method of the invention can completely cover all the genetic mutation of the GJB2 gene base boot sector, the exon 1, the exon 2 and the shear zone, so that detectable rate of the GJB2 genetic mutation and diagnosis rate of hereditary hearing impairment related to the GJB2 gene are improved; compared with that sequence measurement is separately carried out on a GJB2 code area, the method is more comprehensive and reliable, so as to be beneficial to the diagnosis of hereditary hearing impairment.

The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.

A method of producing a fusion protein is provided. The method includes: a) contacting a first protein with a second protein under conditions that enable the formation of an isopeptide bond between said proteins to form a linked protein; and b) contacting the linked protein from (a) with a third protein under conditions that enable the formation of an isopeptide bond between said third protein and said linked protein to form a fusion protein. Peptide linkers and the use of orthogonal pairs of said linkers in the synthesis of fusion proteins are also provided. Recombinant proteins comprising said linkers, nucleic acid molecules encoding said proteins and linkers, vectors comprising said nucleic acid molecules and host cells comprising said vectors and nucleic acid molecules are also contemplated.

The invention relates to a production process for separating and purifying fiber linking proteins from pig blood. The production process comprises the following steps: (1) separating blood plasma and blood cells from whole blood; (2) salting out: charging an acidic Na2HPO4-NaH2PO4 buffer solution into an upper layer of plasma, uniformly mixing, charging ammonium sulfate while stirring the solution so that the saturation degree reaches 20 percent, adjusting pH to be alkaline, placing the mixed solution into a refrigerator, refrigerating the mixed solution for 1.5 to 2.5 hours, sufficiently precipitating, finally centrifuging the solution, and obtaining a main precipitation component, i.e., fiber linking proteins; (3) dialyzing and desalting: dissolving precipitates in the step (2) into an acidic PBS buffer solution, putting the mixed solution into a dialysis bag, carrying out the dialysis and concentration, thereby obtaining a crude product of the fiber linking proteins; and (4) purifying: carrying out the affinity chromatography for an upper sample of the fiber linking protein crude product obtained in the step (3) by virtue of a gelatin sepharose-4B column, and eluting by virtue of a specific volume to obtain the purified fiber linking proteins. The production process is simple, low in requirement on needed equipment and suitable for industrialized production.

A method of treating neurodegenerative disorders of the brain and spinal cord is disclosed. The therapeutic agent is a polyethylene glycol linked protein.

Methods of evaluating the composition of a cell culture (e.g., to distinguish chondrocytes from fibroblasts) and methods for evaluating the phenotype of an individual cell (e.g., as a chondrocyte) are disclosed. The methods may be used, for example, for assessing chondrocyte cultures used for treatment of cartilage defects. In some embodiments, the invention involves identifying cell culture composition or the identity of a cell based on expression level of a fibroblast marker. In other embodiments, the invention involves comparing expression levels of at least one chondrocyte marker and at least one fibroblast marker in a cell culture sample or in an individual cell. In illustrative embodiments, the chondrocyte marker is hyaluronan and proteoglycan link protein 1 (HAPLN1), and the fibroblast marker is microfibrillar associated protein 5 (MFAP5).

The invention provides a seed coating agent capable of efficiently preventing the rape clubroot disease. The seed coating agent comprises, by weight, 1-5 parts of active components, 1-10 parts of film forming agents, 1-10 parts of dispersing agents and 90-100 parts of auxiliaries. The active components include at least one of 6% oligosaccharide link protein, Pulvtong, 3% plant activator protein. After being mixed, the active components, the film forming agents, the dispersing agents and the auxiliaries are smashed till the granularity is not larger than 5 microns, and the seed coating agent is obtained. The seed coating agent has no toxin or harm to soil, people and livestock and has an excellent prevention effect on the rape clubroot disease, the prevention effect can be over 90%, implementation is convenient, and the seed coating agent has good market application prospects.

The present invention provides a method of producing a fusion protein, said method comprising: a) contacting a first protein with a second protein under conditions that enable the formation of an isopeptide bond between said proteins, wherein said first protein and said second protein each comprise a peptide linker, wherein said peptide linkers are a pair of peptide linkers which react to form an isopeptide bond that links said first protein to said second protein to form a linked protein; and b) contacting the linked protein from (a) with a third protein under conditions that enable the formation of an isopeptide bond between said third protein and said linked protein, wherein said third protein comprises a peptide linker which reacts with a further peptide linker in the linked protein from (a), and wherein said peptide linkers are a pair of peptide linkers that react to form an isopeptide bond that links said third protein to said linked protein to form a fusion protein, wherein said pair of peptide linkers used in (a) are orthogonal to the pair of peptide linkers used in (b). Peptide linkers and the use of orthogonal pairs of said linkers in the synthesis of fusion proteins are also provided. Recombinant proteins comprising said linkers, nucleic acid molecules encoding said proteins and linkers, vectors comprising said nucleic acid molecules and host cells comprising said vectors and nucleic acid molecules are also contemplated.

The invention discloses a method for extracting DNA of a single chironomidae pupal stage ecdysis, and belongs to the technical field of animal molecular biology. The method comprises the following steps of pretreatment, pyrolysis centrifugation, elution, enrichment, enzymolysis, extraction, centrifugation, precipitation, dissolution, amplification and electrophoresis detection. Compared with the prior art, the method has the beneficial effects that distilled water is used for treatment to remove impurities and prevent interference extraction; under the influence of slow permeation of a 0.9% NaCl solution, the cells remaining on the chironomidae pupal stage ecdysis can gradually recover physiological activity in a mild state, and gradual dissociation of cross-linked protein and DNA in the cells is promoted; and the concentration of the chironomidae pupal stage ecdysis DNA extracted by the method can reach 0.908 ng / [mu]l, and the purity can reach 1.89.

The invention discloses a cis-platinum cross-linked protein hydrogel and a preparation method thereof. The cisplatin cross-linked protein hydrogel is prepared from the following main components in percentage by mass: 0.5 to 5.0 percent of drug, 6.0 to 50.0 percent of serum albumin and 47.0 to 93.0 percent of solvent medium, wherein carboxyl on the surface of the serum albumin and a drug form a coordinate bond; and the drug is cis-platinum. The hydrogel preparation provided by the invention is simple in structure and easy to prepare, and the used cis-platinum has dual effects: the used cis-platinum is a cross-linking agent for promoting the formation of protein hydrogel and is also an anti-tumor drug for playing a tumor inhibition curative effect. Introduction of other components is effectively reduced, and the potential risk of body side effects is reduced; and on the other hand, the protein carboxyl group limits the release of the cis-platinum through the coordination effect, so that the burst release behavior of the medicine can be reduced to a great extent, and the reduction of the toxicity of the cis-platinum in the body tissue is facilitated.

A polypeptide-human protein-9.68 containing vinculin family characteristic sequence fragment, the polynucleotide for coding it, the process for preparing said polypeptide by DNA recombination, the application of said polypeptide in treating diseases such as digestive ulcer, diabetes, etc, the antagonist of said polypeptide and its medical action, and the application of said polynucleotide are disclosed.

Provided is a method of regenerating cartilage tissues by treatment with hyaluronan and proteoglycan link protein 1 (HAPLN1), and a composition for regenerating cartilage, the composition including HAPLN1 as an active ingredient. According to the present disclosure, the HAPLN1 protein may have cartilage formation-stimulating ability and articular cartilage regeneration ability, may increase an expression level of TGF-β receptor I of chondrocytes to increase a component ratio of cells having cartilage formation ability, and to induce regeneration of cartilage tissues. Accordingly, the HAPLN1 protein of the present disclosure, which is a novel composition regulating TGF-β signaling, may be usefully applied as a pharmaceutical composition for regenerating cartilage, a health food composition for regenerating cartilage, or a reagent composition for regenerating cartilage.

The invention discloses a microcapsule slow-releasing seed coating agent for efficiently preventing and treating clubroots of oilseed rapes. The microcapsule slow-releasing seed coating agent is characterized by being prepared from, by weight, 10 parts to 15 parts of medicine-containing microcapsules, 1 part to 10 parts of film forming agents, 1 part to 10 parts of dispersing agents and 100 parts to 110 parts of assistants; in the medicine-containing microcapsules, the weight ratio of medicine to microcapsule materials is 1:5 to 10; the medicine comprises at least one kind of 6% oligosaccharide.link proteins, Pulvtong and 3% extremely-thin alternaria alternate activator proteins; the microcapsule materials comprise one kind of polylactic acid, polypropylene carbonate and chitosan-lactide copolymers; the medicine-containing microcapsules are prepared with an emulsified solvent evaporation method; the medicine-containing microcapsules, the film forming agents, the dispersing agents and the assistants are mixed to be grinded into particles with the size smaller than or equal to 5 micrometers, and the microcapsule slow-releasing seed coating agent is obtained. The preventing and treating effect of the microcapsule slow-releasing seed coating agent on the clubroots of the oilseed rapes is higher than 90%, and preventing and treating time is longer than eight weeks; the microcapsule slow-releasing seed coating agent is free of damage to the environment and injuries to health of people and livestock and easy to apply, and has good market application prospects.

The invention relates to a regulatory sequence which mediates expression of an operably-linked protein encoding a polynucleotide of interest, wherein the protein encoding polynucleotide is transcribed in leaf tissue and not in pollen. The invention also relates to an expression cassette, vector, and transgenic plant comprising the regulatory sequence.

Provided is a method of regenerating cartilage tissues by treatment with hyaluronan and proteoglycan link protein 1 (HAPLN1), and a composition for regenerating cartilage, the composition including HAPLN1 as an active ingredient. According to the present disclosure, the HAPLN1 protein may have cartilage formation-stimulating ability and articular cartilage regeneration ability, may increase an expression level of TGF-β receptor I of chondrocytes to increase a component ratio of cells having cartilage formation ability, and to induce regeneration of cartilage tissues. Accordingly, the HAPLN1 protein of the present disclosure, which is a novel composition regulating TGF-β signaling, may be usefully applied as a pharmaceutical composition for regenerating cartilage, a health food composition for regenerating cartilage, or a reagent composition for regenerating cartilage.

The present invention relates to a composition, for preventing, relieving or treating cartilage-related diseases or symptoms, comprising hyaluronan and proteoglycan link protein 1 (HAPLN1) as an active ingredient. Specifically, the present invention provides a composition for regenerating cartilage, a composition for preventing, relieving or treating osteoarthritis or a composition for regenerating growth plate cartilage, which comprise HAPLN1 as an active ingredient.

The present invention relates to a pharmaceutical composition for preventing or treating hair loss, comprising hyaluronan and proteoglycan link protein 1 (HAPLN1) as an active ingredient. When administered, the HAPLN1 protein of the present invention grows hair by promoting the proliferation of hair germinal matrix cells through a Ras-ERK1 / 2 signaling pathway activated by a TGF-β protein.

The present invention provides a ProA / G-dRep fusion protein as a universal carrier of nucleic acid-antibody conjugates and its application, and particularly relates to the field of genetic engineering. The ProA / G-dRep fusion protein of the present invention comprises protein A / G fusion protein, flexible peptide and duck circovirus replicase sequentially connected from N-terminus to C-terminus. The ProA / G-dRep fusion protein of the present invention can accurately determine the number of connections between antibodies and single-stranded DNA, has a simple synthesis method, and can be widely used in the research of nucleic acid-protein conjugates.