37 results about "Link protein" patented technology

The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

The present invention provides fusion proteins comprising a first molecule, and a second molecule which is a monovalent immunoglobulin or a fragment of a monovalent immunoglobulin with a long half-life when administered in vivo, methods of making such fusion proteins, pharmaceutical compositions comprising such fusion proteins, and uses thereof.

The invention discloses a double-functional fusion protein based on an antibacterial peptide, a preparation method and an application thereof. The double-functional fusion protein based on the antibacterial peptide is a fusion protein based on a cecropins B protein sequence and a human epidermal growth factor; the linking protein is especially designed to be a locus which can be identified by thrombin; and a purified His tag is constructed on the fusion protein. In the invention, the biological preparation for the fusion protein is realized by using a prokaryotic expression system and the purified fusion protein is obtained. A bacteriostasis test and a verification for realizing live animals are performed by the purified fusion protein to indicate that the fusion protein can be applied to preparing a medicine for treating epidermal wound, can be applied to diseases such as epidermal wound, abrade, burn and scald, etc., and can prevent wound from being infected.

Methods and reagents for site-selective functionalization of peptides and proteins. The methods most generally involve the reaction of a thioester with hydrazine. Reagents include bifunctional reagents of formula:H2N—NH—CH2-M-L-FGand salts thereof where M is a single bond or a chemical group carrying a non-bonding electron pair, such as —C(O)NR′—, where R′ is H, or an alkyl or aryl group; L is an optional linker group as described above; and FG is a functional group having reactivity that is orthongonal to that of the hydrazine group. FG can, among others, be an azide, alkenyl, alkynyl, nitrile (—CN) or triazole group and is preferably an azide group (—N3). Methods and reagents can, for example, be combined with intein-mediated protein splicing to link proteins or fragments thereof to various chemical species or to a surface. Surface immobilization of proteins via the methods herein results in immobilized proteins which substantially retain biological activity and is thus useful for the generation of peptide or protein microarrays. Kits for functionalization and / or immobilization of peptides and proteins are provided as well as microarrays of peptides, proteins or both.

A method of producing a fusion protein is provided. The method includes: a) contacting a first protein with a second protein under conditions that enable the formation of an isopeptide bond between said proteins to form a linked protein; and b) contacting the linked protein from (a) with a third protein under conditions that enable the formation of an isopeptide bond between said third protein and said linked protein to form a fusion protein. Peptide linkers and the use of orthogonal pairs of said linkers in the synthesis of fusion proteins are also provided. Recombinant proteins comprising said linkers, nucleic acid molecules encoding said proteins and linkers, vectors comprising said nucleic acid molecules and host cells comprising said vectors and nucleic acid molecules are also contemplated.

A method of treating neurodegenerative disorders of the brain and spinal cord is disclosed. The therapeutic agent is a polyethylene glycol linked protein.

The present invention provides a method of producing a fusion protein, said method comprising: a) contacting a first protein with a second protein under conditions that enable the formation of an isopeptide bond between said proteins, wherein said first protein and said second protein each comprise a peptide linker, wherein said peptide linkers are a pair of peptide linkers which react to form an isopeptide bond that links said first protein to said second protein to form a linked protein; and b) contacting the linked protein from (a) with a third protein under conditions that enable the formation of an isopeptide bond between said third protein and said linked protein, wherein said third protein comprises a peptide linker which reacts with a further peptide linker in the linked protein from (a), and wherein said peptide linkers are a pair of peptide linkers that react to form an isopeptide bond that links said third protein to said linked protein to form a fusion protein, wherein said pair of peptide linkers used in (a) are orthogonal to the pair of peptide linkers used in (b). Peptide linkers and the use of orthogonal pairs of said linkers in the synthesis of fusion proteins are also provided. Recombinant proteins comprising said linkers, nucleic acid molecules encoding said proteins and linkers, vectors comprising said nucleic acid molecules and host cells comprising said vectors and nucleic acid molecules are also contemplated.

Provided is a method of regenerating cartilage tissues by treatment with hyaluronan and proteoglycan link protein 1 (HAPLN1), and a composition for regenerating cartilage, the composition including HAPLN1 as an active ingredient. According to the present disclosure, the HAPLN1 protein may have cartilage formation-stimulating ability and articular cartilage regeneration ability, may increase an expression level of TGF-β receptor I of chondrocytes to increase a component ratio of cells having cartilage formation ability, and to induce regeneration of cartilage tissues. Accordingly, the HAPLN1 protein of the present disclosure, which is a novel composition regulating TGF-β signaling, may be usefully applied as a pharmaceutical composition for regenerating cartilage, a health food composition for regenerating cartilage, or a reagent composition for regenerating cartilage.

The invention discloses a microcapsule slow-releasing seed coating agent for efficiently preventing and treating clubroots of oilseed rapes. The microcapsule slow-releasing seed coating agent is characterized by being prepared from, by weight, 10 parts to 15 parts of medicine-containing microcapsules, 1 part to 10 parts of film forming agents, 1 part to 10 parts of dispersing agents and 100 parts to 110 parts of assistants; in the medicine-containing microcapsules, the weight ratio of medicine to microcapsule materials is 1:5 to 10; the medicine comprises at least one kind of 6% oligosaccharide.link proteins, Pulvtong and 3% extremely-thin alternaria alternate activator proteins; the microcapsule materials comprise one kind of polylactic acid, polypropylene carbonate and chitosan-lactide copolymers; the medicine-containing microcapsules are prepared with an emulsified solvent evaporation method; the medicine-containing microcapsules, the film forming agents, the dispersing agents and the assistants are mixed to be grinded into particles with the size smaller than or equal to 5 micrometers, and the microcapsule slow-releasing seed coating agent is obtained. The preventing and treating effect of the microcapsule slow-releasing seed coating agent on the clubroots of the oilseed rapes is higher than 90%, and preventing and treating time is longer than eight weeks; the microcapsule slow-releasing seed coating agent is free of damage to the environment and injuries to health of people and livestock and easy to apply, and has good market application prospects.

Provided is a method of regenerating cartilage tissues by treatment with hyaluronan and proteoglycan link protein 1 (HAPLN1), and a composition for regenerating cartilage, the composition including HAPLN1 as an active ingredient. According to the present disclosure, the HAPLN1 protein may have cartilage formation-stimulating ability and articular cartilage regeneration ability, may increase an expression level of TGF-β receptor I of chondrocytes to increase a component ratio of cells having cartilage formation ability, and to induce regeneration of cartilage tissues. Accordingly, the HAPLN1 protein of the present disclosure, which is a novel composition regulating TGF-β signaling, may be usefully applied as a pharmaceutical composition for regenerating cartilage, a health food composition for regenerating cartilage, or a reagent composition for regenerating cartilage.

The invention discloses a double-functional fusion protein based on an antibacterial peptide, a preparation method and an application thereof. The double-functional fusion protein based on the antibacterial peptide is a fusion protein based on a cecropins B protein sequence and a human epidermal growth factor; the linking protein is especially designed to be a locus which can be identified by thrombin; and a purified His tag is constructed on the fusion protein. In the invention, the biological preparation for the fusion protein is realized by using a prokaryotic expression system and the purified fusion protein is obtained. A bacteriostasis test and a verification for realizing live animals are performed by the purified fusion protein to indicate that the fusion protein can be applied topreparing a medicine for treating epidermal wound, can be applied to diseases such as epidermal wound, abrade, burn and scald, etc., and can prevent wound from being infected.

The present invention provides a ProA / G-dRep fusion protein as a universal carrier of nucleic acid-antibody conjugates and its application, and particularly relates to the field of genetic engineering. The ProA / G-dRep fusion protein of the present invention comprises protein A / G fusion protein, flexible peptide and duck circovirus replicase sequentially connected from N-terminus to C-terminus. The ProA / G-dRep fusion protein of the present invention can accurately determine the number of connections between antibodies and single-stranded DNA, has a simple synthesis method, and can be widely used in the research of nucleic acid-protein conjugates.

The present invention relates to a pharmaceutical composition for the prevention or treatment of hair loss, the composition comprising hyaluronan and proteoglycan link protein 1 (HAPLN1) as an active ingredient. When administered, the HAPLN1 protein of the present invention promotes proliferation of hair germinal matrix cells through the Ras-ERK1 / 2 signaling pathway activated by TGF-beta protein, thereby growing hair.