162 results about "Ecdysis" patented technology

The invention provides a medicine preparation for clearing away heat and toxic material, which is composed of following Chinese traditional medicine materials: radix isatidis, chrysanthemum, mulberry leaf, mint, moutan bark, Chinese thorowax root, reed rhizome, cicada ecdysis, taraxacum, gypsum, dried orange peel, leaf of common perilla, cassia seed, rice, magnolia officinalis, figwort, Chinese wolfberry, rhizoma anemarrhenae, forsythia, liquorice, selfheal, gardenia and radix sileris; according to Chinese broad and wide traditional medicine theory and finely selected more than 20 Chinese traditional medicines and made through repeated clinical experiments, the medicine has the curative effects of heat-clearing and fire-purging and removing toxin for detumescence; and the medicine is used for swelling and pain in throat, swelling and pain in gum, constipation, fever heat, thirst, abdominal distention and red tongue caused by inner heat; thus having fine social effects and economical benefits through popularizing widely.

The invention relates to a Chinese medicine preparation for curing tetanus. The formula includes earthworm, cicada ecdysis, rhizoma gastrodiae, notopterygium root, parsnip, herba schizonepetae, rhizoma arisaematis, uncaria, red peony, alum, ophiopogon japonicus, cortex moutan, Indian bread with hostwood, root poria, centipede, whole worm, atrina glass, honeysuckle, hemlock parsley, forsythia, safflower, peach seed and angelica. In the formula, the raw materials are pulverized, filtered and then made into pulvis. During the medicine taking, the medicine is mixed with cold boiled water, with two or three times per day and three days is one treatment period. The Chinese medicine preparation, compared with the prior art, is scientific and reasonable in the formula, simple in the regrouping ofthe raw materials and preparation process, effective in reducing the treatment cost and remarkable in treating tetanus. Having no toxic side effects to the human body, the invention is the medicine prepared commonly in homes.

The invention relates to a traditional Chinese medicine composition for treating allergic rhinitis, which is prepared from the following traditional Chinese medicine raw materials in parts by weight: 10-40 parts of radix astragali, 10-30 parts of angelica, 10-40 parts of dangshen, 10-40 parts of rhizoma atractylodis macrocephalae, 5-20 parts of herba schizonepetae, 5-20 parts of radix sileris, 5-20 parts of cicada ecdysis, 5-20 parts of muscardine silkworm, 10-40 parts of radix paeoniae alba, 5-25 parts of cassia twig, 5-20 parts of magnolia flower, 5-15 parts of cocklebur fruit, 10-30 parts of radix angelicae, 5-25 parts of Baikal skullcap root, 5-25 parts of radix bupleuri, 5-20 parts of balloonflower, 5-20 parts of hemlock parsley, 5-20 parts of notopterygium root, 5-20 parts of mint and 5-15 parts of licorice. The traditional Chinese medicine composition provided by the invention has the effects of clearing away heat and toxic material, promoting the dispersing function of the lung, dredging acupoints, relieving exterior syndrome, dispelling cold, dispersing pathogenic wind and removing dampness, and has obvious curative effect and reliable action on treating allergic rhinitis. The traditional Chinese medicine composition provided by the invention has the advantages of wide medicine sources, simple preparation process, mild drug properties and no toxic or side effect.

The invention belongs to the field of traditional Chinese medicine preparation and relates to traditional Chinese medicine oral liquid for treating dyspepsia of children and a preparation method thereof. The oral liquid of the invention is prepared by processing coix seed, rice sprouts, hawthorn, lophatherum gracile, uncaria, cicada ecdysis, liquorice, flavoring agents and preservatives.

The invention provides an insect cytochrome P450 gene and the application thereof, and particularly relates to the insect cytochrome P450 gene, gene fragments, dsRNA and the application of the dsRNA to killing pests. Firstly, the cytochrome P450 gene of a migratory locust is cloned and sequenced to obtain a CYP4G102 gene, an overall nucleotide sequence of the CYP4G102 gene is SEQ ID NO:1, and an amino acid sequence of the CYP4G102 gene is SEQ ID NO:2; secondly, the gene fragments of which the sequence is SEQ ID NO:3 are obtained and used for synthesizing the dsRNA. After the synthesized dsRNA is injected into the body cavity of the migratory locust, the insect dies due to excessive in-vivo water loss caused by the fact that the water retention capacity of the body wall is reduced after ecdysis, and the death rate reaches 100 percent. The insect cytochrome P450 gene provides a molecular target or pest control based on RNA interference and can be used for the pest control.

The invention provides a Chinese medicine composition preventing and curing varicella, which comprises the following components by weight parts: 4-9 parts of honeysuckle, 4-9 parts of forsythia, 4-9 parts of radix paeoniae alba, 4-9 parts of radix scutellariae, 4-9 parts of gentiana scabra bge, 4-9 parts of radix puerariae, 4-9 parts of platycodon root, 4-9 parts of herba schizonepetae, 4-9 partsof radix angelicae, 6-10 parts of radix isatidis, 6-10 parts of dandelion, 3-7 parts of cicada ecdysis, 3-7 parts of bamboo leaves, 3-7 parts of lithospermum and 3-7 parts of liquorice; and the preparation method is characterized in that all the Chinese medicine components are weighted accurately according to the propotions, crushed, and evenly mixed. The Chinese medicine composition preventing and curing varicella not only can effectively restrain the replication of varicella virus, but also can clear heat, promote eruption, prevent variola from generating in hairless part of the skin and promote the variola from dropping; and the preparation method is simple and is applicable for the requirements of scale industrial production.

The invention discloses a plant extract liquid mixture with antiphlogosis and antianaphylaxis effects. The mixture comprises the following components in percentage by weight: 60-70% of pomegranate extract, 15-20% of witch hazel extract, 5-10% of radix sophorae flavescentis root extract, 5-10% of glycyrrhiza inflata root extract and 5-10% of scutellaria baicalensis root extract. The plant extract liquid mixture disclosed by the invention is safe, gentle and effective in component and has an antiphlogosis and regulation effect on phenomena such as ecdysis, itching, eczema, whelk and pustule which happen easily on anaphylactic skin, and through compounding use, the functions of antiphlogosis, moisture prevention and trophism can be comprehensively brought into play, and not only is a function of relieving the existing symptoms achieved, but also a function of preventing or delaying the anaphylactic skin to be in defective situation is achieved.

The invention discloses a method for extracting collagen by using ecdysis of giant salamander. The method aims at solving the problem that the ecdysis of the giant salamander is wasted so that the resource is seriously wasted in the parturition process of the giant salamander. The method comprises the following steps: pre-treating, coarsely extracting, deodorizing, extracting and purifying collagen. The collagen is finally obtained by treating, performing enzymolysis, filtering, concentrating and spray-drying the ecdysis of the giant salamander in artificial breeding, and the molecular weight is less than 3000 dalton. The ecdysis of the giant salamander is effectively utilized, and the collagen is successfully prepared. The method has a wide market application prospect and good social and economic value.

The invention relates to a traditional Chinese medicine composition for treating wind-heat affecting lung, which comprises the following raw materials in parts by weight: 10-40 parts of honeysuckle, 5-25 parts of fructus forsythiae, 5-20 parts of almond, 5-20 parts of platycodon root, 10-40 parts of ordate houttuynia, 5-25 parts of baical skullcap root, 5-20 parts of herba schizonepetae, 5-20 parts of divaricate saposhnikovia root, 5-20 parts of cicada ecdysis, 5-20 parts of stiff silkworm, 5-20 parts of semen oroxyli, 5-20 parts of fructus chebulae, 5-25 parts of adenophora stricta, 10-40 parts of rhizoma phragmitis, 2-10 parts of tendril-leaved fritillary bulb, 5-20 parts of radix stemonae, 5-20 parts of common coltsfoot flower, 5-20 parts of aster, 10-40 parts of radix peucedani and 5-15 parts of liquorice. The traditional Chinese medicine composition provided by the invention achieves the efficacies of ventilating the lung, suppressing cough, clearing away the lung-heat, reducing phlegm, dispelling wind and heat and clearing away heat and toxic materials, has a remarkable curative effect and a reliable action on treatment of wind-heat affecting lung, is prepared simply and conveniently, has abundant medicinal herbs resources and is low in cost.

The invention belongs to a Chinese medicinal preparation for treating trigeminal neuralgia, which is prepared by raw materials such as rhizoma ligustici wallichii, felwort, dried rhizome of rehmannia, angelica, white paeony root, salvia miltiorrhiza, radix scutellariae, angelica dahurica, scorpion, cicada ecdysis and earthworm. The Chinese medicinal preparation has the advantages of scientific compatibility, low cost, short treatment course, quick response, high cure rate and no side effect on human bodies.

The invention provides a beauty skin care product containing artificially-cultured giant salamander tissue substance or extract, which comprises at least one of giant salamander oil, giant salamander skin secretions, giant salamander ecdysis extract and giant salamander skin tissue extract as additive. The skin care product in the invention has the effects of promoting blood circulation, enhancing skin physiological functions, delaying skin aging, sun block, crack control, whitening and removing maculas, has good effect on treating chilblain and rhagadia, and is an ideal beauty skin care product.

The invention relates to an internal medicine for treating psoriasis, which is characterized in that the formulation thereof comprises angelica, radix polygoni multiflori preparata, red sage root, spatholobus stem, zaocys dhumnade, scorpion, centipede, radix rehmanniae recen, gromwell, radix paeoniae rubra, rhizoma imperatae, pagodatree flower, cicada ecdysis, white tribulus terrestris, radix sophorae flavescentis, radix sileris, night shade, and moutan bark. The internal medicine has the following advantages: 1. the internal medicine is a pure traditional Chinese medicine composite preparation and is reasonably prepared; and 2. all the Chinese herbal medicines used by the preparation have the efficacies of clearing away heat and toxic material, promoting blood circulation and cooling blood, and preserving health and dispersing pathogenic wind, and the effective rate reaches 100 percent.

The invention provides a Chinese traditional medicine produced granule which cures extremity ringworm. The prescription consists of orally taken drug and externally used drug; wherein, the orally taken drug consists of ing-chieh, ledeboruiella root, honeysuckle, burdock, moutan bark, duckweed, root of rehmannia, radix scutellariae, cicada ecdysis, raw liquorice, angelica, primary fissure, hemlock parsley, unpeeled root of herbaceous peony and white peony root; the invention respectively crushes each drug, passing 300 mesh siege and being weighed to be mixed with each drug according to ratio to produce troche, grain or capsule through traditional medicine production craftwork; the externally used drug consists of ageratum, raw rhubarb, polygonatum canaliculatum, alum, pepper powder, stenona root, black-striped snake and balm; the production steps of the externally used drug are that: the ageratum, the raw rhubarb, the polygonatum canaliculatum, the pepper powder and the stenona root are put in sesame oil dipping for 48 hours, then the mixture is heated with gentle fire to 150 to 180 DEG C so as to decoct the drugs to be a little yellowish, filtered while heated, the filtered dregs are ground by adding with the alum to paste with stone roll, evenly the paste is stirred by mixing with oil liquid to paste state and the finished product is got when put into a jar.

The invention discloses a method for fast cultivation of centipedes through a greenhouse, and the greenhouse is established for performing warming-up cultivation in winter so as to break the winter hibernation feature of the centipedes and enable the centipedes to be always in fast growth state. A plastic film is stuck on the wall for effectively preventing escape, flour weevils are fed, and meat, vegetables and the like are used for assistance, thereby providing foods with comprehensive nutrition and sufficient water, and preventing cannibalism. Warming up is performed in the winter, cooling is performed in summer, the temperature is the house is kept at 20-35 DEG C, the water content in culture soil is 15-24%, the atmospheric moisture is 55-75%, and ventilation and air exchange are regularly performed for eliminating diseases and pests. During the whole growth stage of the centipedes, antibiotics, pepsine and ecdysone are often fed, thereby reducing the incidence rate, enabling the ecdysis of the centipedes to be smooth, improving the survival rate and shortening the growth cycle. The centipedes cultivated by adopting the method have the characteristics of high survival rate, short growth cycle, high yield, low cost, high economic benefits and the like.

The invention discloses an intelligent arma chinensis fallou artificial breeding method. The breeding method is characterized by including the step A of adult mating and spawning: placing a female adult and a male adult in an anti-escape mating spawning container in an intelligent climate chamber and putting food and water in the mating spawning container; cleaning up food residue and feces insidethe mating spawning container once a day in the intelligent climate chamber and supplementing the food and the water and collecting egg mass after spawning for artificial hatching. In the environmentof other females and males, mating is only conducted with the paired partners. After the nymph stage and 3-4 days after last ecdysis for becoming adults, the arma chinensis fallou starts pairing andmating in the case of specific ambient temperature and humidity, and the food and the water are supplemented during the pairing.

The neem oil preparation consists of neem oil, water, Tween-60 or Tween-80, Span-60 or Span-80, citric acid, potassium sorbate, EDTA, and nimbin or natural avermectin. The neem oil preparation has high effect of preventing and treating parasitic disease of aquatic animals and no resistance, owing to the functional mechanism including lowering intestinal tract activity, inhibiting ecdysis, inhibiting mating and oviposition, refusing feed, inhibiting egg development, etc. In addition, the present invention has low cost.

The invention provides a migratory locust mesenchymal epidermal protein gene 6 and application thereof in locust control. The method specifically comprises the following steps: acquiring a migratory locust mesenchymal epidermal protein gene from a migratory locust transcriptome by virtue of a bioinformatics method, and further obtaining full-length genes having a sequence of SEQ ID NO:1 by virtueof a migratory locust genomic sequence. dsRNA of the gene is designed and synthesized according to the SEQ ID NO:1, the dsRNA is injected into a body cavity of the migratory locust, so that target genes can be specifically silenced, ecdysis of the body is difficult, the intersegmental membrane cannot be closed, and finally abdomens of injurious insects cannot deep into soil to lay eggs so as to greatly decrease the oocyst number and ovum number. Multiple experiments show that injection of the dsRNA causes reduction of the ovum number of offspring of the migratory locust by 70% or higher. Due to specificity and high efficiency of the gene disclosed by the invention, the gene has an obvious effect of reducing the number of laid eggs of the injurious insects and the offspring population quantity, and a novel pathway can be provided for pest control.

The invention provides a method for collecting selenium enriched toad skins. A toad culture stage comprises the following steps: adding the fodder with the high selenium content 5 to 7 days after the tadpole period, and culturing phycophyta with the high selenium content in the tadpole pond; feeding the bait with the high selenium content and matched fodder in the child toad period; and applying selenium enriched fertilizer to the terrestrial soil of the adult toad pond according to the dosage of 0.4 to 0.6 kg / hm, planting farm crops or vegetables, and feeding artificially cultured bait. A toad skin collecting stage comprises the following steps: adding selenium enriched yeast into the water of an ecdysis pond so as to prevent the selenium contained by the toad from losing; drying the toad skin to the water content less than or equal to 5 percent; and packaging the toad skin into bags after the disinfection through ultraviolet rays so as to obtain the selenium enriched toad skin. The method adds the selenium enriched bait and provides a selenium enriched growing environment in the toad culture process from the child toad to the adult toad, thereby making the toad enriching selenium, and ensuring no loss of selenium in the ecdysis process. Therefore, the toad skin has the high selenium content. The method remarkably improves the medical and functional value of the toad skin.

The invention discloses an Eriocheir sinensis sexual precocity property related SNP marker and its application. The SNP is positioned in a 3'-UTR region of ecdysis inhibition hormone gene MIH(AY310313.1) and is concretely positioned in 3088bp, and base variation information is SNPA3088T T>G. Property-genotype relevance analysis results show that a significant correlation exists between SNP mutation and river crab sexual precocity properties, wherein the sexual precocity property risk of the GG genotype river crab individuals is 2.175 times higher that of TT genotype individuals. The polymorphism SNP marker of the river crab sexual precocity related gene, and can be used for the subsequent and river crab sexual precocity property related genetic and breeding researches.

The invention discloses a traditional Chinese medicine composition for treating allergic rhinitis, which is prepared from the following active pharmaceutical ingredients: radix astragali, rhizoma atractylodis macrocephalae, radix sileris, cocklebur fruit, magnolia flower, herba siegesbeckiae, cicada ecdysis, cornel, fructus psoraleae, lily root, dark plum, radix angelicae, notopterygium root, radix polygonati officinalis, roasted licorice, coix seed, chastetree fruit, Spica Schizonepetae, balloonflower, fructus aurantii, nidus vespae, Tribulus terrestris, Hyssopus officinalis, Ziziphora clinopodioides Lam, wild poppy, Manchurian Lilac, tuber pinellia, groundsel and herba selaginellae. The traditional Chinese medicine composition for treating allergic rhinitis has the effects of invigorating the spleen to benefit the lung, regulating ying and wei, invigorating qi and strengthening the body surface resistance, and is mainly used for treating allergic rhinitis caused by insufficiency of the lung-qi, spleen-qi and kidney-qi; the preparation technique is simple and convenient; and the traditional Chinese medicine composition for treating allergic rhinitis has the advantages of small toxic and side effects and low cost, and is convenient to take and easy to prepare. The traditional Chinese medicine composition can directly get to the focus, and has the advantages of short curing time and low recurrence tendency after curing.

The invention discloses an application of traditional Chinese medicine composition in preparing antihypoxic. The medicament mainly contains ginseng, leech, scorpion, woodlouse, chilopod, cicada ecdysis, red paeonia, sandalwood, dalbergia wood, frankincense, wild jujube and borneol and is prepared perfectly by modern pharmaceutical technique. Tests prove that the traditional Chinese medicament composition can remarkably prolong the survival time of a mouse absence of oxygen and can improve the hypoxia-resistant ability of organism.

The invention discloses a coccinella septempunctata feed as well as a preparation method and application thereof. 10 to 12 parts of sweet water, 8 to 10 parts of fish eggs, 6 to 8 parts of pork liver, 5 to 8 parts of yolk powder, 5 to 6 parts of bamboo flavone, 3 to 4 parts of teasaponin, 4 to 5 parts of vitamin complex, and 4 to 5 parts of olive oil are used as formula raw materials, and the formula raw materials are subjected to homogenate, dipping and mixing, so as to obtain the coccinella septempunctata feed. The coccinella septempunctata feed can replace aphid to be used as feed for coccinella septempunctata artificial feeding, fills the gap of the feed for coccinella septempunctata artificial feeding, can also improve the adaptability and the disease resistance of coccinella septempunctata and improve the biological control capacity, and can also be used as feed additive during the ecdysis of the coccinella septempunctata larva, and is mixed with aphid larva to feed coccinella septempunctata, so as to replenish the nutrition consumed during the ecdysis and promote the larva to grow fast.

The invention belongs to the field of economical animal cultivation, in particular to a method of improving the scorpion ecdysis speed and the ecdysis scorpion survival rate. The method comprises the following steps of according to the following mass proportions, taking 15-35% of plant straw particles, 5-15% of soil particles, 15-25% of coal ash particles, and the balance of saw dust particles; uniformly mixing the raw materials, and placing the mixed raw materials into a cave opening of a scorpion nest. The friction between oil skin and the outside is increased by the uniformly mixed particle raw materials when the scorpion is subjected to ecdysis, the separation speed of the body and the old skin is accelerated, the particle raw materials are accumulated in the cave opening of the nest under the instinct effect of the scorpion, the trace of the scorpion which is subjected to the ecdysis can be covered, and the probability that the scorpion which is subjected to the ecdysis is killed by other scorpions is reduced.

The invention belongs to the technical field of medicine healthcare, and particularly relates to a sachet for removing moisture and relieving exterior syndromes and a preparing method thereof. The sachet comprises, by weight, 20-30 parts of eupatorium fortunei turcz, 20-40 parts of wrinkled gianthyssop herb, 20-30 parts of rhizoma atractylodis, 10-20 parts of folium artemisiae argyi, 20-40 parts of mint, 10-15 parts of borneolum syntheticum, 10-20 parts of mulberry leaves, 15-45 parts of chrysanthemum flowers, 5-10 parts of bombyx batryticatus, 10-20 parts of herba plantaginis, 15-35 parts of agalmatolite, 5-10 parts of cicada ecdysis, 10-30 parts of fructus aurantii, 10-20 parts of rhizoma pinelliae, and 5-10 parts of radix scrophulariae. The preparing method comprises the steps of smashing the chrysanthemum flowers and folium artemisiae argyi to prepare chrysanthemum-folium artemisiae argyi velvet, preparing agalmatolite, bombyx batryticatus and borneolum syntheticum into internal pellets and afterwards preparing pills through the internal pellets and other herbs, using the chrysanthemum-folium artemisiae argyi velvet to wrap the pills, and then loading the wrapped pills into non-woven bags to prepare medicine bags. Through the formula of herbs in the medicine bags, the fragrance of the medicine bags lasts longer; meanwhile, the sachet has the effects of relieving exterior syndromes, dispelling cold, and removing moisture, and meanwhile the sachet is cool and refresh, and can prevent summer heat-dampness cold.

The invention discloses a traditional Chinese medicine for treating eczema, which is characterized by comprising the following Chinese herbs as main components: 15g of forsythia, 15g of lopseed, 10g of phellodendron, 6g of rhizoma coptidis, 10g of root-bark of tree peony, 5g of ophiopogon roots, 20g of oriental bittersweets, 6g of batryticated silkworms, 10g of cicada ecdysis, 15g of frutus cnidii, 10g of fructus gardeniae, 10g of rhubarb, 6g of liquorice, and the like. A preparation method of the traditional Chinese medicine for treating eczema comprises the following steps: putting the Chinese herbs into a container, dipping the Chinese herbs into water for 30min, frying the Chinese herbs by slow fire for 30min, filtering residues, and extracting 50ml of decoction. One dose of the traditional Chinese medicine is taken after the dinner every day, and three doses are used as one course of treatment. The prescription is prepared from pure Chinese herbs by a traditional preparation method, remains the property of the Chinese herbs, has the efficacy of clearing away heat and toxic material, removing dampness and destroying parasites and dispelling wind and arresting itching, achieves the aim of treating and has no side effect.

The invention relates to a traditional Chinese medicine composition for treating measles and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 13-17 parts of carrots, 9-11 parts of coriander, 9-11 parts of rhizoma phragmitis, 6-8 parts of platycodon root, 6-8 parts of almonds, 6-8 parts of honeysuckle, 6-8 parts of folium mori, 6-8 parts of fructus forsythiae, 6-8 parts of folium isatidis, 2-4 parts of radix bupleuri, 2-4 parts of fructus aurantii, 2-4 parts of divaricate saposhnikovia root, 2-4 parts of fructus arctii, 2-4 parts of cicada ecdysis, 2-4 parts of mentha haplocalyx, 2-4 parts of dried orange peel, and 2-4 parts of liquorice. The traditional Chinese medicine composition has the advantages of remarkable curative effect, small side effect and simplicity in preparation method.

The invention discloses a traditional Chinese medicine preparation for treating infantile eczema. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: 2-8 parts of burdock, 5-15 parts of radix rehmanniae recen, 10-20 parts of densefruit pittany root-bark, 8-18 parts of honeysuckle, 2-10 parts of mint, 2-8 parts of akebiaquinata, 1-5 parts of liquorice, 1-5 parts of coptis chinensis, 2-8 parts of rush, 2-10 parts of cicada ecdysis, 2-10 parts of chrysanthemum, 5-12 parts of fructus xanthil, 8-15 parts of fructus cnidii, 8-18 parts of broom cypress fruit, 8-20 parts of heartleaf houttuynia herb and 5-15 parts of radices lithospermi. According to the invention, the raw materials are wide in sources, and low in price; the traditional Chinese medicine preparation provided by the invention has clinically assured efficacy on treatment of the infantile eczema, and effectively reduces severity of skin damage (ADS) in children patients, and both the short-term treatment effect and the long-term treatment effect are obviously better than those in a control group; the preparation method provided by the invention is simple and feasible, and the prepared preparation is convenient to take and use, and is easy to accept by family members of the children patients.

The scorpion rapid growth and feeding technology described in the present invention belongs to the field of economic animal breeding, including stocking density, food feeding, water supply, feeding management of pregnant scorpions, feeding management of young scorpions, molting of scorpions, mating of scorpions and production of scorpions. young. The invention has the beneficial effects of realizing large-scale breeding of scorpions and increasing the survival rate and output ratio of scorpion breeding.

The invention discloses a kit and for detecting virus infecting situation of Chinese giant salamander colony iridovirus by use of ecdysis. The kit comprises 1 tube of solution A, namely ecdysis sample lysis solution containing PBS (phosphate buffer saline) and 1% triton-100, 1 tube of solution B containing 200mug / ml of protease K, 1 tube of solution C, namely template extraction solution containing phenol, chloroform and isoamyl alcohol based on the proportion of 25:24:1, 1 tube of solution D containing 3mol / l sodium acetate and the like. In the invention, the fresh Chinese giant salamander ecdysis is taken as a pathological material detection sample, thus solving the defect that the detection sample is obtained by killing Chinese giant salamander individuals; according to the characteristics of the Chinese giant salamander ecdysis, the method for extracting virus genome DNA from the ecdysis is optimized, and the kit for simply and effectively extracting an iridovirus gene from the Chinese giant salamander ecdysis sample as well as 2 pairs of PCR (polymerase chain reaction) primers for 2 conserved genes of the iridovirus and PCR conditions are designed; and positive and negative reference substances are established. By utilizing the kit for detection in standard steps, the virus infecting situation of the Chinese giant salamander colony iridovirus can be more reliably mastered, thus being favorable for application to actual production.

The invention discloses a traditional Chinese medical composition for treating allergic conjunctivitis, and belongs to the field of traditional Chinese medicine. The traditional Chinese medical composition is prepared from the following drug substances in parts by weight: 8-12 parts of ash bark, 8-12 parts of concha haliotidis, 8-10 parts of natural indigo, 10-15 parts of wild chrysanthemum flowers, 8-10 parts of plantain seeds, 8-12 parts of dandelion, 6-10 parts of rhizoma anemarrhenae, 6-10 parts of broom cypress fruits, 5-8 parts of honeycombs, 5-8 parts of cicada ecdysis, 5-8 parts of Asiatic moonseed rhizome, 5-8 parts of fragrant solomonseal rhizome, 6-10 parts of selfheal, 5-8 parts of poria cocos and 5-8 parts of bunge corydalis herb. The traditional Chinese medical composition adopts natural traditional Chinese medicine as raw materials, is short in treatment course, definitive in therapeutic effect and free of toxic and side effects, can effectively cure allergic conjunctivitis, and prevents relapse of allergic conjunctivitis, and the total effective rate reaches up to 98%.