52 results about "Arma chinensis" patented technology

The invention discloses a large-scale arma chinensis fallou artificial breeding method. The method comprises the following steps that A, adults are mated and lay eggs; B, an egg mass is hatched; C, 2-5-age nymphs are fed; D, the adults are fed and released. The inventor dedicates to research the biological characteristics, the predatory function and artificial feeding of arma chinensis fallou fora long time, and large-scale feeding of the arma chinensis fallou is achieved through small tests and pilot tests; by the end of 2017, more than one million pieces of arma chinensis fallou are obtained; the large-scale breeding method is efficient, the land is saved, the cost is low, and the method is convenient to popularize.

The invention discloses an intelligent arma chinensis fallou artificial breeding method. The breeding method is characterized by including the step A of adult mating and spawning: placing a female adult and a male adult in an anti-escape mating spawning container in an intelligent climate chamber and putting food and water in the mating spawning container; cleaning up food residue and feces insidethe mating spawning container once a day in the intelligent climate chamber and supplementing the food and the water and collecting egg mass after spawning for artificial hatching. In the environmentof other females and males, mating is only conducted with the paired partners. After the nymph stage and 3-4 days after last ecdysis for becoming adults, the arma chinensis fallou starts pairing andmating in the case of specific ambient temperature and humidity, and the food and the water are supplemented during the pairing.

The invention relates to the technical field of biological control of agricultural pests, in particular to a breeding method of arma chinensis fallou with yellow mealworm pupae as a host, and providesa prey more suitable for artificial large-scale breeding of the arma chinensis fallou and a feeding method to reduce the mortality of the arma chinensis fallou and improve the acquisition rate of adult arma chinensis fallou.The method comprises the following steps: (1) artificial breeding of yellow mealworms; (2) selection of the yellow mealworm pupae; (3) refrigerated preservation; (4) breedingof one-year-old arma chinensis fallou: an egg-collecting cup containing eggs of the arma chinensis fallou is placed in a breeding device of the arma chinensis fallou for incubation; when the eggs of the arma chinensis fallou begin to hatch, absorbent cotton is soaked with honey water with the concentration of 5%, the gauze of the egg-collecting cup is covered with the absorbent cotton for the armachinensis fallou to eat; (5) breeding of 2-5-year-old arma chinensis fallou: the yellow mealworm pupae are fed into the breeding device of the arma chinensis fallou for feeding; (6) pairing of the adult arma chinensis fallou and egg laying : one male and one female of the adult arma chinensis fallou are selected and put into a plastic cup, and two yellow mealworm pupae are put into the cup; (7) hatching of the eggs of the arma chinensis fallou.

The present invention provides a predatory nature enemy arma chinensis artificial feed, and further discloses a preparation method for the predatory nature enemy arma chinensis artificial feed, wherein vitamins, amino acids, fats, carbohydrates, proteins and other raw materials are mixed to prepare the predatory nature enemy arma chinensis artificial feed, the predatory nature enemy arma chinensis artificial feed is provided for large-scale nature enemy production, and production cost is reduced while the number and continuity of insect source supply are ensured. The breeding results show that a breeding survival rate is high, a cost is low, application market prospects are broad, and economic benefits, ecological benefits and social benefits are significant.

The invention relates to a method for prolonging life of adult arma chinensis. The method includes following steps: 1), putting newly-generated arma chinensis eggs into a centrifugal tube; 2), addingsterilized deionized water into the centrifugal tube in the step 1), and putting the centrifugal tube into an oscillator for at least three times of oscillation treatment; 3), adding sterilized deionized water into the centrifugal tube in the step 2), and putting the centrifugal tube into the oscillator for oscillation treatment; 4), adding sterilized deionized water into the centrifugal tube in the step 4), and putting the centrifugal tube into the oscillator for oscillation treatment; 5), using sterilized deionized water to flush and clean the arma chinensis eggs obtained in the step 4), andusing sterilized filter paper to blot up water on the surface. After arma chinensis eggs treated through the above experiment steps are hatched to form adults, life of the adults is obviously prolonged in lab conventional feeding conditions, so that improving of propagation of arma chinensis population is facilitated, and economic cost, time cost and labor cost of arma chinensis feeding are saved.

The invention discloses an arma chinensis breeding method. According to the method, silkworm chrysalis is used as a food source for arma chinensis breeding. The arma chinensis breeding method providedby the invention is more suitable for artificially breeding arma chinensis on a large scale, mortality rate of the arma chinensis can be reduced, egg laying amount and the breeding additional value of the arma chinensis can be increased, the survival rate of arma chinensis nymphs and imagoes in the long-term breeding process is 95% or above, and the single-female egg laying amount is 300 or above.

The invention discloses a method for detecting the egg laying amount of insects by a vitellogenin gene, which provides application of a substance which is used for detecting the expression quantity of vitellogenin encoding genes of insect populations eating different substances to detection of the egg laying amount of insect populations eating different substances, wherein the amino acid sequence of vitellogenin is a sequence 2 in the sequence list. Experiments prove that the detection method provided by the invention can be used for detecting the egg laying amount of the insects and particularly the egg laying amount of arma chinensis with different nutrition sources, and the egg laying amount of the insects can be detected within only 2 to 3 days by the detection method while the detection requires 30 to 60 days by a traditional biological method. The detection method provided by the invention has the advantages that materials have wide sources and are easily available, and the operation is simple as well as time-saving and labor-saving, thus being suitable for popularization and application in insect commodity inspection and detection.

The invention relates to a method for prolonging the shelf life of arma chinensis eggs. The method comprises the step (1) and / or the step (2), wherein (1) parent arma chinensis eggs are treated by using a solution with a disinfecting effect and water, hatching, feeding, mating and oviposition are conducted, and breeding is performed under a shading condition; (2) the arma chinensis eggs are subjected to gradient cooling and storage under the shading condition. The method has the advantage that the storage life of the arma chinensis eggs is long, and the method has important significance on production of arma chinensis and preservation of germplasm resources.

The invention discloses a method for detecting the self-mutilation rate of insects by using an odorant binding protein 15 gene. The invention provides an application of a substance for detecting the expression level of an odorant binding protein 15 coding gene in insect populations fed with different substances in detection of the self-mutilation rate of the insect populations fed with different substances; and the amino acid sequence of an odorant binding protein 15 is sequence 2 in a sequence table. According to the method disclosed by the invention, experiments prove that the detection method provided by the invention can be used for detecting the self-mutilation rate of the insects, in particular to the self-mutilation rate of arma chinensis with different nutrient sources; and the detection of the self-mutilation rate of the insects by the detection method disclosed by the invention only requires 2-3 days. However, the use of a traditional biological method requires about 30-60 days. The detection method disclosed by the invention has the advantages of wide range of involved material sources, easiness in purchase, simplicity in operation, and time-saving and labor-saving properties, and is suitable for popularization and application in commodity inspection and detection of insects.

The invention relates to a method of storing arma chinensis. The method primarily comprises the following steps: cleaning and disinfecting a storage place, preparing a storage place environment, carrying out gradient cooling, storing arma chinensis at a low temperature, providing food to arma chinensis, cleaning the storage place and the like. The method of storing arma chinensis is simple to operate, provides a good path for feeding and storing arma chinensis in a mass propagation period, shortens the arma chinensis feeding time effectively and lowers the labor cost effectively, and has important meaning in storing and feeding the arma chinensis.

The invention discloses a matching method of Arma chinensis by artificial rearing. After Arma chinensis peel and become adult for 3 to 4 days, Arma chinensis in a breeding utensil is observed. 2 female and male Arma chinensis satisfying one of the following conditions are taken out and separably placed into the breeding utensil for matching and breeding such that natural mating and spawning are achieved. The method comprises following steps: (1) ones which are mating; (2) ones overlapped with each other; (3) ones which are chasing and determine they are close. The method can be used for scale breeding of Arma chinensis production so that pair mating rate can be up to more than 90% and defects in the prior art are overcome.

The invention discloses a method for cold storage of Arma chinensis adults, which comprises the following steps: considering the low-temperature resistance of Arma chinensis adults, carrying out short-term low-temperature adaptation on Arma chinensis adults with a certain eclosion time in a refrigeration house, and then putting Arma chinensis adults into the refrigeration house with lower temperature for medium-term and long-term storage; when Arma chinensis adults need to be used, moving the Arma chinensis adults out of the refrigeration house and gradually heating to the normal temperature.Therefore, Arma chinensis adults can be stored for a medium and long term and can be used for preventing and controlling pests at any time.

The invention relates to a method for improving the raising effect of arma chinensis by adding substitute host plants. According to the method, pea seedlings or broad bean seedlings are adopted as thehost plants for raising arma chinensis. The method comprises the following steps that the host plants are added into a raising space, and the arma chinensis is inoculated. In the method, host plants(such as poplar or elm branches and leaves) used in a traditional arma chinensis raising process are replaced with the broad bean seedlings or the pea seedlings which are easier to obtain, a host plant cultivation method in the raising process is improved, and the service life of the host plants is effectively prolonged. The method is easy to operate, the difficulty of obtaining the host plants for predatory natural enemy insect arma chinensis adults is greatly reduced, the arma chinensis raising effect is improved, and the method has an important significance in large-scale propagation and field release of the natural enemy insect, namely arma chinensis.

The invention discloses a simulated phytotron for Arma chinensis breeding, comprising a house body which is closed inside. A Mythimna separata larva raising device, an Arma chinensis larva breeding device, an Arma chinensis adult feeding device, a temperature control device and a humidity control device are arranged in the house body. The simulated phytotorn aims to solve the problem of low survival rate of Arma chinensis in artificial raising in the prior art.

The present invention relates to an artificial feed for arma chinensis. The artificial feed for arma chinensis comprises, in parts by weight, 45-60 parts by weight of egg liquid, 4-6 parts by weight of armyworms or oak silkworms, 4-6 parts by weight of vegetable oil, 4-5 parts by weight of cane sugar, 0.020-0.030 parts by weight of sitosterol and 0.004-0.045 parts by weight of a preservative; andcomprises, in parts by volume, 80-120 parts by volume of milk, wherein the ratio of 1 part by weight of the milk to 1 part by volume of the milk is 1.02-1.04 g / ml. The ingredients of the invention areeasier to obtain, and the feed has more stable quality, lower chemical additive content, and lower price. A small amount of components including the armyworms or the oak silkworms are added to the feed so as to better improve the trace nutrient components in the feed required by arma chinensis. The chemical additive type is reduced so as to reduce the toxicological effect of the chemical additiveon arma chinensis. The present invention also relates to a preparation method of the artificial feed for arma chinensis.

The invention belongs to the technical field of insect control, and particularly relates to a natural enemy insect storage and release device. The natural enemy insect storage and release device comprises a natural enemy insect box, an isolation gauze and an oriental armyworm box, wherein the bottom of the natural enemy insect box and the top of the oriental armyworm box are each provided with four connecting holes in an annular array, four connecting columns are fixedly connected to the upper surface and lower surface of a fixing ring in an annular array, and the natural enemy insect box is connected with the oriental armyworm box through the isolation gauze. According to the natural enemy insect storage and release device, the oriental armyworm box and the isolation gauze are arranged, so that the natural enemy insect box is connected, arma chinensis in the natural enemy insect box has sufficient food sources in the transportation and storage process, and the survival rate of the arma chinensis is improved; and the design of an easy-to-tear strip not only facilitates release of the arma chinensis, but also can connect a pull ring with the natural enemy insect box, so that the device can be conveniently hung on crops, the food sources in the oriental armyworm box have certain attraction to the arma chinensis, the arma chinensis can be effectively attracted and left nearby andis prevented from flying away, and insect pest control is guaranteed.

The invention discloses an arma chinensis larva breeding device. The device comprises a fixed base, a box body is fixedly connected to the upper end of the fixed base, a groove is formed at the innerbottom of the box body, a breeding box is slidably connected into the groove, and two symmetrically-arranged box covers are slidably connected to the upper end of the box body; and sliding grooves areformed in the two inner side walls of the box body. By arranging a lifting mechanism, lifting of a feeding box is achieved, when food in the feeding box needs to be replaced, only the box covers needto be opened, the feeding box can be automatically lifted, feeding and cleaning by workers are facilitated, by arranging an opening and closing mechanism, a rotating plate can automatically close vent holes in rainy days, rainwater is prevented from entering the breeding box, death of arma chinensis larvae and economic losses are avoided, a collection mechanism is arranged, rainwater can be effectively collected on rainy days, water resources are reasonably utilized, and waste of the water resources is avoided.

The invention relates to a breeding method of arma chinensis, and belongs to the technical field of insect breeding and biological pest control. According to the method, a time relation of growth anddevelopment of food-borne plants, prey insects and the arma chinensis is combined, the modern agricultural science and technology are adopted according to biology and ecology habits of the food-borneplants, the prey insects and the arma chinensis, coordinated development of the food-borne plants, the prey insects and the arma chinensis is achieved, manual operation is reduced as much as possible,and rolling feeding of the arma chinensis is achieved. The method has the advantages of being easy to operate, low in cost, high in survival rate, high in intensification degree, capable of facilitating large-scale breeding and easy to popularize.

The invention discloses an arma chinensis cold storage diapause method. The method comprises the following steps that (1) arma chinensis armyworm eggs are selected as cold storage diapause objects; and (2) the arma chinensis armyworm eggs selected in the step (1) are placed in a cold storage diapause environment, wherein the cold storage diapause temperature is 2-6 DEG C, and the cold storage diapause time is 8-12 days. Aiming at arma chinensis which is an excellent predatory natural enemy insect, the control research of regulating and controlling the arma chinensis armyworm egg incubation time by utilizing factors such as cold storage temperature and time is carried out, the research finds that the incubation rate of the arma chinensis armyworm eggs still reaches 50% or above after the arma chinensis armyworm eggs stand for 6-14 days in the cold storage diapause environment with the temperature of 0-8 DEG C, and large-scale cultivation requirements can be basically met.

The invention discloses a method for detecting the egg laying amount of insects by a heat shock protein 90 gene, which provides application of a substance which is used for detecting the expression quantity of heat shock protein 90 encoding genes of insect populations eating different substances to detection of the egg laying amount of insect populations eating different substances, wherein the amino acid sequence of heat shock protein 90 is a sequence 2 in the sequence table. Experiments prove that the detection method provided by the invention can be used for detecting the egg laying amount of the insects and particularly the egg laying amount of arma chinensis with different nutrition sources, and the egg laying amount of the insects can be detected within only 2 to 3 days by the detection method while the detection requires 30 to 60 days by a traditional biological method. The detection method provided by the invention has the advantages that materials have wide sources and are easily available, and the operation is simple as well as time-saving and labor-saving, thus being suitable for popularization and application in insect commodity inspection and detection.

The invention relates to the technical field of insect breeding devices, in particular to an ecological insect breeding box which comprises box bodies, insect put-in openings, humidity keeping devicesand host plant devices. A first clamping part is arranged at the upper end of each box body, and a second clamping part is arranged at the lower end of each box body. A food put-in opening is formedin each box body and provided with a food adding device. The insect put-in openings are formed in the box bodies. The humidity keeping devices are arranged at the insect put-in openings and connectedwith the box bodies. The host plant devices are arranged in the box bodies at intervals. In the scheme, the vertically adjacent box bodies can be overlapped together through the clamping parts, the breeding space can be used to the maximum extent when the box bodies are overlapped in the breeding process, cost input is reduced, the host plant devices can provide host plants for insects, reduce thechange frequency of the host plants, increase the activity area of Arma chinensis Fallou and reduce the autotomy conditions of the Arma chinensis Fallou, and the yield of advanced-age nymph and adultinsects in the large-scale breeding process of the Arma chinensis Fallou is increased.

The invention discloses a large-scale arma chinensis fallou artificial breeding method. The method comprises the following steps that A, adults are mated and lay eggs; B, an egg mass is hatched; C, 2-5-age nymphs are fed; D, the adults are fed and released. The inventor dedicates to research the biological characteristics, the predatory function and artificial feeding of arma chinensis fallou fora long time, and large-scale feeding of the arma chinensis fallou is achieved through small tests and pilot tests; by the end of 2017, more than one million pieces of arma chinensis fallou are obtained; the large-scale breeding method is efficient, the land is saved, the cost is low, and the method is convenient to popularize.

The invention discloses a multi-layer feeding method and a device for feeding arma chinensis by prey insects. The device comprises an arma chinensis feeding net-shaped container (1) for living of the arma chinensis, wherein an armyworm living net-shaped container (2) is sheathed at the exterior of the arma chinensis feeding net-shaped container (1), or more than one armyworm living net-shaped container is adhered to the exterior of the arma chinensis feeding net-shaped container (1). The feeding device has the advantages that the prey insects and the arma chinensis can be separated, the cleaning of prey insect corpses is convenient, the time is saved, and the labor intensity is decreased; the space is fully utilized, and the feeding efficiency is improved; the whole device is made of a transparent yarn net, the air ventilating and transmittance effects are better, and the scaled feeding of the arma chinensis is realized.

The invention discloses an automatic collection device for arma chinensis eggs. The device comprises a frame body, a guide rail with left and right sides facing is distributed evenly at the bottom ofthe frame body, a drawer used for breeding arma chinensis is installed on the guide rail, a gauze winding wheel is arranged on one side of the frame body, rollers are arranged at both ends of the upper side of the frame body, the gauze of the gauze winding wheel is connected with a gauze collecting wheel at the other end of the frame body through the lower tangent line of the roller, the rotatingshaft of the gauze collecting wheel is connected with a motor, the frame body at the motor end is provided with a device for scraping and collecting arma chinensis eggs, a movable cover plate is arranged on the frame body, the movable cover is close to the upper surface of the gauze, and the gap between the lower surface of the gauze and the upper end surface of the drawer is larger than the diameter of arma chinensis eggs, and is smaller than the thickness of the adult arma chinensis. According to the present invention, the device can facilitate uniform collection of arma chinensis eggs, andthe working efficiency can be improved.

The invention discloses a method for increasing egg laying quantity of arma chinensis and collecting arma chinensis eggs, and belongs to the technical field of biological control. According to the method for increasing egg laying quantity of arma chinensis and collecting arma chinensis eggs disclosed by the invention, through regulating a photoperiod, the egg laying quantity of the arma chinensiscan be notably increased, and the egg laying period can be shortened; and through homeopathic egg picking action, the difficulty of collecting egg granules can be greatly reduced, massive egg granulescan be quickly, efficiently and conveniently collected, egg block scattering cannot be caused, damage is also not easy to cause, and when raising condition is appropriate, the hatching rate of the egg granules can be guaranteed to be 95% or above.

The invention provides a field colonization method for searching a natural enemy arma chinensis. The field colonization method for searching the natural enemy arma chinensis comprises the following steps of S1, uniformly coating fluorescent paint on a pronotary and tegmina of the natural enemy arma chinensis by using a banister brush, putting the arma chinensis into an insect breeding cage, and taking the natural enemy arma chinensis to the field for releasing after the fluorescent paint is dried; and S2, irradiating from the releasing position by using a fluorescent flashlight at night after releasing, searching the arma chinensis, and investigating the diffusion amount of the arma chinensis and the field distribution condition of the arma chinensis. The fluorescent paint is evenly coated on the pronotary and the tegmina of the natural enemy arma chinensis, the arma chinensis is released in the daytime, then the fluorescent flashlight is used for irradiating and searching the arma chinensis from the releasing position at night, and the released arma chinensis can be found conveniently; after the fluorescent paint is used for marking, the diffusion condition of 60% or above of target insects can be investigated, the tracking effect is improved by four times or above, the tracking effect is improved, the effect is good, operation is easy, and efficiency is high.

The invention relates to a method for increasing the egg laying amount of arma chinensis. The method comprises the following steps that 1) newly laid arma chinensis eggs are placed in a centrifuge tube; 2) after an ethanol solution is added into the centrifuge tube in step 1), and the centrifuge tube is placed in an oscillator for three-time oscillation treatment; 3) after a formaldehyde solutionis added into the centrifuge tube in step 2), the centrifuge tube is placed in the oscillator for oscillation treatment; 4) after the ethanol solution is added into the centrifuge tube in step 3), thecentrifuge tube is placed in the oscillator for oscillation treatment; 5) after the obtained arma chinensis eggs in step 4) are rinsed thoroughly with a sterilized deionized water, the surface moisture is sucked dry with sterilization filter paper. The method for increasing the egg laying amount of arma chinensis has the advantages that after the arma chinensis eggs treated through the experimental steps are hatched into adult arma chinensis, under the conventional breeding conditions of a laboratory, the egg laying amount is effectively increased, thereby being conductive to the expanding propagation of arma chinensis population, and saving the economics, time and labor cost of arma chinensis raising.

Arma chinensis, as an important predatory natural enemy, has a good control effect on many lepidopteran and coleopteran pests. The invention provides a method for biologically preventing and treatinggrassland spodoptera frugiperda by the arma chinensis. The method includes the following steps: (1) monitoring a free-range breeding area, (2) performing general survey on the free-range breeding area, (3) breeding the arma chinensis in a free range, (4) tracking pest conditions, and (5) providing supporting plans. The method can control the grassland spodoptera frugiperda through biological prevention and treatment means of 'treating pests by insects', the control effect is more than 80%, the pesticide consumption is reduced, and social and ecological benefits are good.

The invention discloses a method for detecting the self-mutilation rate of insects by using an odorant binding protein 15 gene. The invention provides an application of a substance for detecting the expression level of an odorant binding protein 15 coding gene in insect populations fed with different substances in detection of the self-mutilation rate of the insect populations fed with different substances; and the amino acid sequence of an odorant binding protein 15 is sequence 2 in a sequence table. According to the method disclosed by the invention, experiments prove that the detection method provided by the invention can be used for detecting the self-mutilation rate of the insects, in particular to the self-mutilation rate of arma chinensis with different nutrient sources; and the detection of the self-mutilation rate of the insects by the detection method disclosed by the invention only requires 2-3 days. However, the use of a traditional biological method requires about 30-60 days. The detection method disclosed by the invention has the advantages of wide range of involved material sources, easiness in purchase, simplicity in operation, and time-saving and labor-saving properties, and is suitable for popularization and application in commodity inspection and detection of insects.

The invention discloses a method for detecting the egg laying amount of insects by a heat shock protein 83-1 gene, which provides application of a substance which is used for detecting the expression quantity of heat shock protein 83-1 encoding genes of insect populations eating different substances to detection of the egg laying amount of insect populations eating different substances, wherein the amino acid sequence of heat shock protein 83-1 is a sequence 2 in the sequence table. Experiments prove that the detection method provided by the invention can be used for detecting the egg laying amount of the insects and particularly the egg laying amount of arma chinensis with different nutrition sources, and the egg laying amount of the insects can be detected within only 2 to 3 days by the detection method while the detection requires 30 to 60 days by a traditional biological method. The detection method provided by the invention has the advantages that materials have wide sources and are easily available, and the operation is simple as well as time-saving and labor-saving, thus being suitable for popularization and application in insect commodity inspection and detection.