The invention relates to the field of aquatic feed, in particular to special formula feed for improving tilapia mossambica meat quality and a preparation method thereof. Ingredients in a feed formula comprise, by weight, 40-70 parts of broad bean, 2-5 parts of fish meal, 7-12 parts of bran, 9-19 parts of rapeseed dregs, 8-19 parts of peanut meal, 1-2 parts of fish oil, 0.3-0.5 part of glycine betaine, 0.4-0.6 part of choline chloride, 0.6-0.8 part of monocalcium phosphate, 0.10-0.13 part of methionine, 0.06-0.1 part of enzyme preparation, 0.02-0.03 part of allicin, 0.4-0.6 part of multivitamins and 1.0-1.2 parts of complex mineral. The preparation method of the feed is further provided. The feed can improve the tilapia mossambica meat quality to be firm, hard, crisp and tasty, improves growth performance of tilapia mossambica, reduces pollution on water quality and production cost and remarkably improves economical and social benefit.