The invention relates to aquaculture biological fish manure, in particular to novel high-efficiency biological fish manure for various aquacultures. The fish manure is prepared from the following raw materials in parts by weight: 17.5 to 70 parts of ammonium chloride, 10 to 40 parts of monoammonium phosphate, 7.5 to 30 parts of calcium superphosphate, 12 to 48 parts of organic matters, 0.5 to 2 parts of bacillus, and 2.5 to 10 parts of humic acid. The fish manure is prepared by selecting more than twenty types of major and trace element inorganic fertilizers with comprehensive nutrition and organic fertilizers which can be easily absorbed, is rich in more than twenty types of major and trace elements and various amino acids, vitamins, polysaccharide, nucleic acids, humic acid and the like, and is fermented by 81 beneficial microorganisms in five major groups of microorganisms existing in the whole ecosystem. The fermented free-state nutritional substances are advantageous to absorption and utilization, so that the nutrition balance of a water body is ensured. By application, a small ecological environment is formed, so the biological fish manure is particularly suitable for growing and breeding of plankton which facilitates aquaculture.