2455 results about "Glass slide" patented technology

A method and apparatus for an automated biological reaction system is provided. In the processing of a biological reaction system, there is a need for consistently placing an amount of liquid on a slide. In order to accomplish this, several methods are used including a consistency pulse and a volume adjust means. Moreover, in order to reliably operate an automated biological reaction system, the dispenser must be reliable, easy to assemble and accurate. Among other things, in order to accomplish this, the dispense chamber is substantially in line with the reservoir chamber, the reservoir chamber piston is removed, and the flow of liquid through the dispenser is simplified. Further, in order to operate the automated biological reaction system more reliably, the system is designed in modular pieces with higher functions performed by a host device and the execution of the staining operations performed by remote devices. Also, to reliably catalog data which is used by the automated biological reaction system, data is loaded to a memory device, which in turn is used by the operator to update the operator's databases. The generation of the sequence of steps for the automated biological reaction device based on data loaded by the operator, including checks to determine the ability to complete the run, is provided.

The present invention is a handle for controlling the deflection of a distal end of a catheter body. The catheter body includes first and second deflection wires that extend through the catheter body from the distal end of the catheter body. The handle comprises a slide base, an adjustment knob, a first slide and a second slide. The slide base includes a first end, a second end, and a slide compartment longitudinally extending through at least a portion of the slide base. The adjustment knob is rotateably connected to the first end of the slide base and includes a hole extending through the knob, wherein at least a portion of an inner diameter of the hole includes an internal right thread and an internal left thread. The first slide is located in the slide compartment, is adapted to be coupled to the first deflection wire, and includes an external right thread. The second slide is located in the slide compartment, is adapted to be coupled to the second deflection wire, and includes an external left thread. The internal threads of the knob engage the threads of the slides. Consequently, in operation, rotation of the adjustment knob causes the slides to displace in opposite directions within the slide compartment and the distal end of the catheter body to deflect accordingly.

The slide handler is an instrument that automatically transfers glass microscope slides from a cassette or magazine to a motorized microscope stage and then returns the slide back into the second cassette. The use of this instrument permits the unattended computer control, measurement and inspection of specimens mounted to the slides. Full modular integration of the system components allows for the slide handler instrument to be utilized with any microscope. The instrument system has a minimum of three components; namely a slide cassette indexer, an XY-stage, and a slide exchange arm. The indexer, the arm and the XY-stage are connected together and integrated into one unitary modular instrument that can be moved from one microscope to another.

A method and system for providing a fluidic interface to slides bearing microarrays of biomolecules or other samples immobilized thereon to perform a variety of chemical reactions or processing steps on the slide. An interface device seals against the slide to form a chamber or chambers containing all or a portion of the microarray, providing selective access to portions of the slide. The interface device includes inlet and outlet ports permitting liquid sample and reagents to be introduced to and removed from the chamber accessing the slide surface. Pre- and post-array microfluidic circuitry may be included in the interface device or in attachable modules. The system may include one or more compartments for collecting and storing waste fluids.

A compact device for operatively coupling a solid planar substrate, for example a glass slide, to a microfluidic circuit and performing a reaction or reactions on organic matter bound to the face of the planar substrate. Typical reactions include binding, staining and / or labeling reactions. In use, a sealed reaction chamber is formed, the chamber enclosing the organic matter and at least a part of the solid substrate. Headspace in the sealed chamber between the solid substrate is generally of microfluidic dimensions, and diaphragm pump members are used to inject, exchange and / or mix the fluids in the chamber.

A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.

The invention provides a full-automatic medical examination blood piece pushing and dyeing machine to solve the problems of a medical examination blood piece pushing and dyeing technology in the prior art that the manual operation ratio is high, the labor intensity is great, the confusion is easy to cause, the examination quality is difficult to completely guarantee and the like. The full-automatic medical examination blood piece pushing and dyeing machine comprises a rack, a base plate, a test tube conveying device, a barcode reading device, a glass slide conveying device, a sample coating device, a piece pushing device, a printing device, a glass slide transferring device, a dyeing device, a glass slide collecting device, a control system, a dyeing solution container, a washing solution container and a waste solution container. The full-automatic medical examination blood piece pushing and dyeing machine disclosed by the invention has the beneficial technical effects that a flow line of test tube conveying, barcode reading, glass slide conveying, blood sample coating, barcode information printing, sample dyeing and glass slide collection is formed so that the operations of uniform mixing, coating, piece pushing, dyeing and collection of a blood sample can be automatically finished, samples can be prevented from being confused and the detection reliability is guaranteed.

This invention relates to substrates for use in immobilizing biomolecules. More particularly, the invention relates to substrates (e.g. glass slides) having a coating of polylysine covalently attached to a silane layer coating the slide, wherein the polylysine compound has a functional NH2 group which can be coupled directly, indirectly, covalently, or non-covalently to a biomolecule (e.g., a DNA or RNA molecule). Even more particularly, the invention relates to specific prescribed addition of ethanalomine to the polylysine thereby forming a mixture which dramatically enhances the effectiveness of the polylysine for immobilizing DNA. Among other applications, the polylysine coated substrates can be used in the preparation of high density arrays for performing hybridization assays

A method of capturing a focused image of a continuously moving slide / objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.

A hand-operated device for measuring, dispensing and storing of powder, granular and grain materials, having filling and discharging / storing positions, comprising: a container wherein the material is stored and a measuring and dispensing unit attached to the container. In the preferred embodiment the unit includes: a housing having interconnected material receiving and material discharging openings; a slide moveable back and forth inside the passageway, delivers the material from the receiving opening to the discharging opening, accommodating a predetermined volume of the material dispensed by the device in one stroke; extensions prevent bridging of the dispensed material, a spring, being extended when the slide moves inside the housing due to an outside force applied to the slide and returning the slide into its original position after the outside force is released; a retaining apparatus holding the slide inside the housing in its discharging position when the device is not in use, a stopping apparatus fixing filling position of the slide; and apparatus providing airtight closing of the measuring and dispensing unit of possible penetration of air from the container to outside atmosphere or back. In the first embodiment the device includes extensions on the slide directed towards the container to break-up clogged material within container during movement of the slide. In the second embodiment the transporting mechanism includes also a screening mechanism capable to close or open the compartment in the slide wherein the dispensed material is received.

The invention discloses a preparation method and application of a polyaniline / titanium dioxide / graphene conductive composite membrane. The preparation method comprises the steps of: adding 3-60wt% of titanium dioxide, 0.05-5wt% of graphene and 0.6-10wt% of polyaniline into protonic acid solution, and adopting an in-situ polymerization method to obtain a polyaniline / titanium dioxide / graphene conductive composite; sequentially and evenly coating conductive resin and polyaniline / titanium dioxide / graphene coatings to matrixes such as polypropylene, glass slides, metal plates and the like, wherein each coating is 30-500mu m thick; and drying at 60-80 DEG C to obtain the needed product. According to the preparation method, the titanium dioxide is embedded in a conductive material to prepare the membrane, the defects that the nano TiO2 is not easy to recover and the quantum yield is low can be overcome, the separation efficiency of photoproduction electrons and cavities can be improved, the photoresponse range can be extended, and the photocatalytic efficiency can be improved.

Systems and methods for creating and viewing three dimensional virtual slides are provided. One or more microscope slides are positioned in an image acquisition device that scans the specimens on the slides and makes two dimensional images at a medium or high resolution. This two dimensional images are provided to an image viewing workstation where they are viewed by an operator who pans and zooms the two dimensional image and selects an area of interest for scanning at multiple depth levels (Z-planes). The image acquisition device receives a set of parameters for the multiple depth level scan, including a location and a depth. The image acquisition device then scans the specimen at the location in a series of Z-plane images, where each Z-plane image corresponds to a depth level portion of the specimen within the depth parameter.

A slide holder (6) for use with a microscope to accurately locate a slide (19) in position under the microscope has a pivotal lever (13) having a clamping and locating portion (14) with abutments (15) and (16) which bear on respective edges of the slide when it is in the clamped position. Further abutments (10) on a fixed angular plate (9) locate the other edges of the slide. The slide holder is adapted for mounting on a slide translation stage of a slide loader (1) which includes a stationary sensor block (20) positioned to engage the lever (13) when the slide holder moves from its inspection or scanning position under the microscope to a loading / unloading position. The slide translation stage includes a robotic head for lifting and depositing slides and the translation stage includes a bar code reader to read a bar code on the slide.

The invention particularly relates to a circulating tumor cell detection kit, a preparing method thereof and an application thereof. The circulating tumor cell detection kit comprises a kit body, detection reagents and a micro-fluidic chip, wherein the detection reagents and the micro-fluidic chip are arranged in the kit body. The micro-fluidic chip is formed by a PDMS substrate and a glass slide in an attached mode. A micro-channel is distributed on the surface of the PDMS substrate, and a fishbone-shaped alternate type mixing structure is arranged on the bottom face of the micro-channel. After the PDMS substrate and the glass slide are bonded, a microfluid channel is formed on the surface of the micro-channel and the surface of the glass slide, and capturing probes are loaded on the surface of the micro-channel. The invention further provides the preparing method of the micro-fluidic chip and a method for detecting circulating tumor cells through the kit. Compared with the prior art, the circulating tumor cell detection kit and the methods have the advantages that the capture probes are loaded on all the surfaces of the micro-channel, and therefore the capturing efficiency is improved; as the micro-fluidic chip is modified through single strand nucleotide, the storage time of the chip is prolonged; in addition, a cell-fixing-free straining method is provided, and therefore convenience is provided for further cell gene sequencing.

The invention discloses a device and a method for classifying microalgae in ship ballast water. The device comprises a micro-fluidic chip carrying platform, a micro-fluidic chip, direct current driving assemblies, an excitation light source, light filtering assemblies, light detection assemblies and data processing assemblies, wherein the micro-fluidic chip comprises a glass slide, a liquid storage hole A, a liquid storage hole B, a liquid storage hole C, a liquid storage hole D, a liquid storage hole E, a main channel, a first focusing channel, a second focusing channel, a detection channel, an impedance pulse sensing upstream detection channel and an impedance pulse sensing downstream detection channel. By using the micro-fluidic chip as a ship ballast water microalgae classification micro-platform, the device is convenient to carry and high in detection speed. Relevant photoelectric detection equipment can be in a small-size structural form and can be conveniently transported to the field. Therefore, compared with existing large classification equipment, the device has the advantages of small size, light weight, convenience in carrying and the like, and can be handheld for quick field classification.

The invention discloses a micro-fluidic chip for generating micro-droplets the sizes of which are uniform, and belongs to the technical field of experimental devices and methods. The elastic bottom surface T-shaped micro-fluidic chip mainly comprises a dispersed phase inlet, a side channel, a continuous phase inlet, a main channel, an outlet, a main solid structure, a film bottom surface structure, a base and a glass slide. Two incompatible liquids for generating the micro-droplets can flow in from the dispersed phase inlet and the continuous phase inlet and are joined on a joint of the side channel and the main channel, a dispersed phase breaks to form the droplets which can flow to the downstream along with a continuous phase and finally flow out of the chip through the outlet; the film bottom surface structure deforms and vibrates under the liquid action, a droplet generation process is influenced, and the micro-droplets with good uniformity are obtained. The micro-fluidic chip provided by the invention can utilize a simple T-shaped micro-channel structure to generate micro-scale droplets with good uniformity, which cannot be generated by a general T-shaped micro-fluidic chip, without an extra driving or control device, the micro-fluidic chip is intuitive and distinct, and the operation is simple.

Systems and methods for improving quality assurance in pathology using automated quality assessment and digital image enhancements on digital slides prior to analysis by the pathologist are provided. A digital pathology system (slide scanning instrument and software) creates, assesses and improves the quality of a digital slide. The improved digital slide image has a higher image quality that results in increased efficiency and accuracy in the analysis and diagnosis of such digital slides when they are reviewed on a monitor by a pathologist. These improved digital slides yield a more objective diagnosis than reading the corresponding glass slide under a microscope.

A composite modular system for handling biological sample vials and slides comprises a vial scanner configured to read indicia on the sample vials, a slide labeler configured to mark the slides, a cap manipulator configured to uncap and cap the vials, and a controller, where all other elements are in communication with the controller and the system outputs the uncapped vial and the marked slide.

The invention relates to a preparation method and application of zwitterion water gel dressing. According to the preparation method, zwitterion monomers, cross linking agents, initiators, sodium chloride and distilled water are uniformly mixed according to the ingredient content; a glass slide is used for preparing templates; a polyfluortetraethylene pad is clamped between two glass slides, and a gap is then formed there; gauze is cut into the proper size to be clamped between the glass slides; then, a uniformly mixed reaction solution is dripped into the template gap; through crosslinking reaction, the zwitterion water gel modified gauze dressing can be obtained. Identically, during the preparation of a zwitterion water gel woundplast, the zwitterion water gel modified woundplast dressing can be obtained through the crosslinking reaction. The water gel dressing provided by the invention has the advantages that the water content is high, so that the wound is in a moist environment; meanwhile, the water absorption capability is high, and the secreted body fluid can be absorbed at any time, so that no excessive pyema exists in the wound position; high biocompatibility is realized; the problems of skin allergy inflammation and the like cannot be caused; the fast heeling of the wound can be promoted.

The present invention relates to a slide glass and others for providing color information as comparative references that can be used in color evaluation and color correction of an image of a measured sample taken with a microscope. The slide glass is used in the microscope and used for acquiring an image of a measured sample, and has a glass plate on which a sample is to be mounted, and one or more micro color filters placed on one surface of the glass plate. Each of these micro color filter groups has two or more color-reference micro color filters for acquisition of colors as comparative references to be used in the color evaluation and color correction of the image. The color-reference micro color filters belonging to an identical micro color filter group are placed so as not to overlap with each other on one surface of the glass plate, and have at least three reference colors different from each other among all hues. The size of each of these color-reference micro color filters belonging to an identical micro color filter group is adjusted in a range where it can be captured within a field of view acquired in use of an objective lens of the microscope.

Improved composite microarray slides for use in micro-analytical diagnostic applications are disclosed. Specifically, composite microarray slides useful for carrying a microarray of biological polymers on the surface thereof including composite microarray slides having a porous membrane formed by a phase inversion process effectively attached by covalent bonding through chemical agents that comprise anchor / linker moieties to a substrate that prepares the substrate to sufficiently bond to the porous membrane formed by a phase inversion process such that the combination produced thereby is useful in microarray applications and wherein the composite microarray slides are covalently bonded to a solid base member, such as, for example, a glass or Mylar microscope slide, such that the combination produced thereby is useful in microarray applications. Apparatus and methods for fabricating the composite microarray slides are also disclosed.

The invention relates to testing field of cell pathology. It aims at providing a flaking dyer and a method to flak and dye by this dyer. The dyer contains dye platform (on which set dye chamber with slide in it, pipette and test-tube rack containing test-tubes), loading rack connects with dye platform vertically, mobile device connects with loading rack which has transfer head and a set of conduct pipes. The device integrates dying and flaking to realize automatization of the whole process. It operates conveniently and can flak many pieces at one time, which is suitable for large hospitals and testing centers specially. Pipette retreat device and reclaiming device of the dyer have retinal institution, simple structure and convenient operation.

Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

A method for preparing oligonucleotide chip for transgene product detection and use are disclosed. It includes: designing specific oligonucleotide probe and related primer by heterogeneric inserting gene, species internal standard gene, specific boundary sequence in transgene product gene set, fixing probe on glass slide to form transgene product detecting chip, amplifying DNA of plant to be tested by related primmer, marking by fluorescence, and hybridizing with chip. It can be use to acquire variable information.

The invention discloses a preparation method of a super hydrophobic coating based on super hydrophobic silica and resin. The resin as a substrate is coated on a glass slide; the super hydrophobic silica is bonded to the resin; and the resin is cured to prepare the super hydrophobic coating. The super hydrophobic coating prepared by the invention is composed of a base resin and a super hydrophobic silica surface layer. The body of the super hydrophobic silica layer is a super hydrophobic material, and if the surface is worn, the coating still has the same super hydrophobic property. When the super hydrophobic coating is polluted, the super hydrophobic function can be recovered by scraping off the surface layer. The super hydrophobic coating provided by the invention also can achieve waterproof effect due to the base resin.

The invention relates to an immunofluorescence staining method for suspension cells. The immunofluorescence staining method for the suspension cells is characterized by comprising the following steps of: collecting cells into a centrifuge tube; performing 800-gram centrifugation to collect the cells; respectively adding paraformaldehyde to fix, Triton-X100 to pass through and 1 percent bovine serum albumin (BSA) to close; adding a primary antibody and a secondary antibody sequentially to incubate; washing by an 800-gram centrifugation method after the operation of each step; performing diamino phenyl indole (DAPI) staining; and dripping on a glass slide and closing the glass slide to observe. The immunofluorescence staining method for the suspension cells has the advantages that: the immunofluorescence staining process is performed in the centrifuge tube, so the problems that the suspension cells grow on the glass slide difficultly and drop off easily in the test process are solved; the staining result is good; and the method is suitable for the suspension cells and cells which are adhered to the wall difficultly.