Slide-making staining machine and method for slide-making staining method

Abstract

The invention relates to testing field of cell pathology. It aims at providing a flaking dyer and a method to flak and dye by this dyer. The dyer contains dye platform (on which set dye chamber with slide in it, pipette and test-tube rack containing test-tubes), loading rack connects with dye platform vertically, mobile device connects with loading rack which has transfer head and a set of conduct pipes. The device integrates dying and flaking to realize automatization of the whole process. It operates conveniently and can flak many pieces at one time, which is suitable for large hospitals and testing centers specially. Pipette retreat device and reclaiming device of the dyer have retinal institution, simple structure and convenient operation.

Description

technical field [0001] The invention relates to the field of cytopathological detection, in particular to a staining machine for cell slice preparation and staining and a method for staining of cell slices. Background technique [0002] Cells are the basic structural and functional units that make up the human body. By observing the changes and abnormal phenomena of cells in various tissues of the human body, we can know whether there is a disease in the human body. Cell preparation is the key to cytopathological detection. For example, the most common method for confirming the diagnosis of female cervical or vaginal lesions is to take a smear of exfoliated cells, fix them, stain them, and examine them under a microscope. One of the existing cell smears is the traditional Pap smear. Although the Pap smear is widely used, there are 6-55% false negative results. The main reason is that there are certain problems in the preparation of the specimen, such as the diseased cells a...

AI Technical Summary

Problems solved by technology

However, since the diluted sample is directly filtered by the filter membrane, and the sample liquid is rotated and mixed at a high speed during film production, it is drawn with negative pressure and passed through a specific filter membrane, and different densities (resistance) are prepared with a fixed negative pressure. specimens, it is easy to cause the blood mucus in the specimen to block the filter membrane, resulting in insufficient number of cells on the slice, or even white flakes

At the same time, the mucus adheres to the membrane and is transferred to the thin slice during film production. Moreover, when the filter membrane and the glass slide cannot be completely attached, the cells collected on the membrane will be lost, which will affect the accuracy of interpretation of the film.

In addition, the external force during the above-mentioned production process can destroy the cell morphology, resulting in uncontrollable production quality

In addition, this kind of TCT is a simple film production system, which is produced one by one in order. After the film production is completed, manual dyeing is required, which leads to the deterioration of the standardization and uniformity of dyeing, and also affects the film production. efficiency

Moreover, this kind of TCT equipment is expensive, and the cost of experimental consumables is high

[0004] In addition, the recovery device for the pipette used to transfer liquid in the existing film maker is set in the recovery box at the lower left corner of the staining platform, so the moving path of the pipette head used to transfer the pipette is long, resulting in It takes time and affects the work efficiency, and the pipe withdrawing device and the detection device for detecting whether the pipette head has a reliable connection with the pipette are all set at the pipette head, making the entire pipette head bulky and structurally Complicated, inconvenient to move, and poor detection effect

[0005] In addition, the mechanical arm of the existing tablet machine is easy to vibrate, resulting in easy leakage of the pipette and affecting the cleaning of the table

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