The invention discloses a
fluorescence labeling method for detecting
DNA matters of a cotton
pollen mother
cell, and belongs to the biological technical field. The method comprises the following steps: on a sunny
morning, buds are picked up, and a sample passes through
ethanol with reducing concentrations to enter
distilled water;
pollen mother cells with a
diameter of less than 7mm of the buds are placed in glycerin for infiltration for 2 to 3 hours, and, after washing, are dyed by
DAPI in the dark for 1 to 2 hours to
label DNA matters in the cells; mature
pollen grains with the
diameter of more than 7mm of the buds are added with a 10 percent
sodium hypochlorite aqueous solution, and subjected to water bath treatment at a temperature of 60 DEG C for 10 minutes, washed, placed in 0.1 to 0.2 percent glycerin for infiltration for 2 to 3 hours, washed, added with
DAPI solution, stood for 10 minutes and subjected to microscopic examination to
label DNA matters in the cells. The method has the advantages of simple and quick operation, less damages to cells, good labeling effect and easy observation, and can play an important role in research on the development process of cotton male
gametophyte, and has high application value.