108 results about "DAPI" patented technology

The invention relates to an immunofluorescence staining method for suspension cells. The immunofluorescence staining method for the suspension cells is characterized by comprising the following steps of: collecting cells into a centrifuge tube; performing 800-gram centrifugation to collect the cells; respectively adding paraformaldehyde to fix, Triton-X100 to pass through and 1 percent bovine serum albumin (BSA) to close; adding a primary antibody and a secondary antibody sequentially to incubate; washing by an 800-gram centrifugation method after the operation of each step; performing diamino phenyl indole (DAPI) staining; and dripping on a glass slide and closing the glass slide to observe. The immunofluorescence staining method for the suspension cells has the advantages that: the immunofluorescence staining process is performed in the centrifuge tube, so the problems that the suspension cells grow on the glass slide difficultly and drop off easily in the test process are solved; the staining result is good; and the method is suitable for the suspension cells and cells which are adhered to the wall difficultly.

A biopsy-sized tissue sample is stained for quick imaging. A significant amount of permeation enhancer is included in a mixed solution of permeant enhancer, fixative or dehydrant, and one or two fluorescent dyes to simultaneously dehydrate and dye the tissue sample. The permeation enhancer, e.g., 10% to 50% in the mixed solution, achieves an image of dyed tissue in the contacted tissue sample at a depth of at least 200 um within no more than 1.5 hours. One of the fluorescent dyes is a fluorescent nuclear dye such as DAPI, SYTOX green, acridine orange, propidium iodide, or a Hoechst dye. The other fluorescent dye is a fluorescent protein dye such as eosin or rhodamine B. The tissue sample is cleared with a clearing agent having a refractive index of at least 1.4[R2], e.g., using BABB. The mixed solution may further include Chloroform or other morphology preservative.

The invention discloses a method for detecting reactive oxygen content in living cells through DAPI fluorescent dye. According to the method, the DCF fluorescence signal values of the single living cells corresponding to exposed contaminants at different concentrations can be obtained. According to the method for detecting the reactive oxygen content in the living cells, the detecting result is precise, the problem that due to the fact that the contaminants are exposed, the number of the cells changes, and final fluorescence intensity measurement is inaccurate is solved, the fluorescence signal values of the single living cells corresponding to a certain concentration can be truly reflected through the method for detecting the reactive oxygen content, therefore, the damage of contaminant exposure to the cells can be truly and accurately reflected, and a more solid foundation is laid for application of an in-vitro cell model in contaminant toxicity assessment.