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161 results about "H&E Staining Method" patented technology

Automatic sputum smear staining instrument for medical use and staining method thereof

The invention discloses a medical self-sputum smear dyeing device and method instead of artificial operation in the tuberculosis detection technique domain, which comprises the following parts: three-dimensional right-angle coordinate mechanical console, cam, smear operation head, dyeing liquor sprayer, sampling tag box component, sample box location board, slide location rack, pusher box, creeping pump group, and display and operation button. The method comprises the following steps: finishing automatic box opening, shooting and image display; selecting the suspected part of sputum sample artificially; gathering the sample to coat on the slide; dying and drying fractionally; preparing the smear sputum sample to satisfy the microscopic requirement. The invention prevents the detecting personnel from infection and medical pollution, which improves the quality of sputum smear and shortens the sample disposal time.
Owner:王海波

A method for immunofluorescence staining of suspension cells

The invention relates to an immunofluorescence staining method for suspension cells. The immunofluorescence staining method for the suspension cells is characterized by comprising the following steps of: collecting cells into a centrifuge tube; performing 800-gram centrifugation to collect the cells; respectively adding paraformaldehyde to fix, Triton-X100 to pass through and 1 percent bovine serum albumin (BSA) to close; adding a primary antibody and a secondary antibody sequentially to incubate; washing by an 800-gram centrifugation method after the operation of each step; performing diamino phenyl indole (DAPI) staining; and dripping on a glass slide and closing the glass slide to observe. The immunofluorescence staining method for the suspension cells has the advantages that: the immunofluorescence staining process is performed in the centrifuge tube, so the problems that the suspension cells grow on the glass slide difficultly and drop off easily in the test process are solved; the staining result is good; and the method is suitable for the suspension cells and cells which are adhered to the wall difficultly.
Owner:RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Hematoxylin eosin staining solution and preparation method thereof

The invention provides a hematoxylin eosin staining solution, comprising a hematoxylin staining solution and an eosin staining solution, wherein the hematoxylin staining solution comprises hematoxylin, an alumium salt, an oxidizing agent, benzalkonium chloride, an alcohol, a weak acid and water; the eosin staining solution comprises eosin, Biebrich scarlet, flame red B, water and ethanol. The invention also provides a preparation method of the hematoxylin eosin staining solution, and a dyeing method thereof. When the hematoxylin eosin staining solution provided by the invention is used for dyeing slices, the effects of clear background, distinct gradation, good dyeing effect, easy slice reading, not easy fading and convenience for long-term storage can be achieved.
Owner:无锡市江原实业技贸有限公司

SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) coomassie brilliant blue R250 fast staining solution, staining method and application

The invention discloses SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) coomassie brilliant blue R250 fast staining solution, which includes from 20 to 40% of ethanol by volume percentage, from 5 to 10% of carbinol by volume percentage, from 0.5 to 1.25g / L of CBB (coomassie brilliant blue) R250 by mass concentration, from 2.5 to 5% of phosphoric acid by volume percentage and from 100 to 200g / L of ammonium sulfate by mass concentration. A fixing step and a sensitization step before staining are omitted, protein staining time can be shortened, and the possibility that protein samples are polluted is greatly reduced. The invention further discloses a method for staining SDS-PAGE gel by the aid of the staining solution and application of the staining solution in a protein two-dimensional gel electrophoresis and protein western blot qualitative diagnosis and / or quantification process.
Owner:INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI

Method for staining medical tissue slice

The invention provides a medical tissue section staining method which comprises the following steps that: olefin is sliced into sheets, dewaxed and hydrated; sliced sheets are put into solution of picric acid, formaldehyde and glacial acetic acid after being heated, taken out and washed by running water; the sliced sheets are put into a 5 percent sodium thiosulfate solution to soak and washed by distilled water; the sliced sheets are put into an alcian blue solution to soak and washed by the running water; the sliced sheets are put into a preheated alkaline ethanol solution to soak and washed by the running water; under the lighttight condition, the sliced sheets are put into a haematoxylin working solution and washed by the running water and the distilled water; the sliced sheets are put into a saffron / acid fuchsine working solution and washed by the distilled water; the sliced sheets are put into a phosphotungstic acid solution to soaked, then transferred to the glacial acetic acid to soak and washed by the distilled water; the sliced sheets are dehydrated by ethanol; the sliced sheets are put into an ethanol saffron solution to soak, then dehydrated by the ethanol and sealed. The medical tissue section staining method has low cost and vivid staining, shortens the time for a staining flow and also increases the application range.
Owner:SHANDONG UNIV

Staining method for aramid fiber

The invention provides a staining method for aramid fiber. The staining method comprises the steps of preprocessing, preshaping, staining, reduction clearing, color fixing, soft processing, drying, shaping, calendaring and rolling. According to the staining method, DEET is added in to serve as a dye carrier, and the staining effect and efficiency are improved; meanwhile, substitute alkali is adopted to replace a conventional soda substance, and not only is the usage amount decreased, but also the color fixing effect is enhanced to enable the washability of the aramid fiber is further enhanced; the reduction clearing and color fixing processes are added, and therefore the staining fastness is good; green and environmental-friendly non-formaldehyde TCD-R is adopted in the color fixing process, the fiber is dried step by step after color fixing, and therefore the firmness of the fiber is greatly improved; the soft processing process is added, the color fastness and softness of fabric are improved, the roughness of the fabric is reduced, and therefore the fabric is fluffy and rich in elasticity.
Owner:SUZHOU ZHILVE INTELLECTUAL PROPERTY OPERATION CO LTD

Construction method and application of osteoporosis zebra fish model

The invention belongs to the technical field of medicines and particularly relates to a construction method and an application of an osteoporosis zebra fish model. The construction method comprises the following steps: constructing transgenetic zebra fish with green fluorescence of a skeleton report after obtaining a promoter of a specific transcription factor of osteoblast by virtue of applying a tol2-gateway technique; constructing the glucocorticoid induced osteoporosis zebra fish model by using the transgenetic zebra fish; and meanwhile, estimating the model by applying a bone-strengthening and kidney-tonifying traditional Chinese medicine herba epimedii general flavone. The osteoporosis model constructed by the method provided by the invention is convenient, feasible and effective, and is suitable for high throughput screening of anti-osteoporosis drugs. Compared with the conventional chemical staining methods, the construction method is more stable, time-saving and efficient.
Owner:HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE

Staining kit and preparation method, staining method, and use thereof

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need oftwo main, and its detection accuracy rate is high, can be up to 98%.
Owner:裴芳君

Method for dyeing pearl

The invention relates to a dyeing method of a peal, which includes the preparation of a staining agent, the pretreatment of a peal and the dyeing the peal. Firstly, pretreatment is carried out to the staining agent and the peal to lead the bonding force between the staining agent and the peal to be greatly improved and simultaneously the peal is not damaged, thus ensuring that the peal is not easy to fade in a short time and improving the luster of the peal thereby promoting the level of the peal. Furthermore, the dyeing fluid of the peal can be repeatedly used for once or twice, thus decreasing cost and reducing pollution.
Owner:浙江千足珠宝有限公司

Floating sphere immunofluorescent staining method and staining device

The invention discloses a floating sphere immunofluorescent staining method and a staining device, belonging to methods for coloring a sample for test. The staining method comprises the following steps: separation of spheres from a culture medium, fixing by stationary liquid, adding of Triton X-100 transparent cells, closing by confining liquid, adding of an interest protein-resisting primary antibody working solution, adding of a primary antibody-resisting fluorescent secondary antibody working solution, and cell nucleus staining by DAPI dye liquor. The staining device comprises a cell chamber and a staining sleeve part arranged at the outer part of the lower end of the cell chamber in a sleeving way. The floating sphere immunofluorescent staining method has the advantages of high efficiency, simpleness, convenience and the like, an antibody usage amount is reduced, experiment cost is reduced, manpower and time are saved, an accurate and reliable experiment result is obtained on the basis that a fundamental principle that immunofluorescent staining is not changed and specially training experimenters is not needed, sphere loss in an experiment operation process is avoided, an experiment success rate is increased, and the efficiency is improved.
Owner:GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV

Coomassie brilliant blue staining solution and staining method

The invention discloses a coomassie brilliant blue staining solution and a staining method. The coomassie brilliant blue staining solution comprises 0.1-10 percent by volume of acid, 1-15 percent by volume of ethyl alcohol, 10-50g / L of soluble starch and 20-1000mg / L of coomassie brilliant blue aqueous solution. The staining solution does not contain reagents harmful to human bodies and is environmental friendly. The staining method using the staining solution has the beneficial effects that due to addition of the soluble starch, the staining background of gel can be reduced, a protein band can be specifically stained, the sensitivity of staining is effectively improved, and therefore, the whole staining process of the protein band can be completed within 20 minutes by using the staining method, the steps such as gel fixation, sensitization and destaining in a conventional dyeing process are omitted, operating steps are greatly simplified, and the dyeing time is saved; the staining method has the advantages of short cycle, high sensitivity and small background interference.
Owner:BEIJING VICNOVO SCI TECH

Staining solution of transparent dying of timbers or bamboos and dying method thereof

The invention relates to a dying solution of transparent dying of timbers or bamboos and a dying method thereof, and the dying solution comprises traditional timber or bamboo special dye and accessoryingredient consisting of methanol and water, wherein each liter of the dying solution has 0.1-10g of the special dye; the water in the accessory ingredient is less than or equal to 90% in volume percent, the water in less than or equal to 70% is preferable, and the water in less than or equal to 35% is more preferable. The dying method in the invention has simple operation and needs no pretreatment on timbers or bamboos such as bleaching and the like.
Owner:赵斌

Preparation method of mangosteen shell natural dye, application and staining method thereof

The invention discloses a preparation method of a mangosteen shell natural dye, application and a staining method thereof. The preparation method of the mangosteen shell dye includes: subjecting a mangosteen shell to washing, air drying and mechanical crushing, then adding the crushed mangosteen shell into an ethanol water solution containing sodium hydroxide, performing reflux extraction at 60-90DEG C for 0.5-2h to obtain an extracted solution, then carrying out filtration and reduced pressure distillation on the extracted solution so as to obtain the concentrated mangosteen shell natural dye. For the ethanol water solution, the mass content of ethanol is 20-40%, per kilogram of the ethanol water solution contains 0.5-1.5g of sodium hydroxide, the crushed mangosteen shell and the ethanol water solution are in a mass ratio of 1:10-30, and the mass of the concentrated mangosteen shell natural dye accounts for 1 / 6 to 1 / 2 of the mass of the ethanol water solution. The extraction process of the mangosteen shell natural dye involved in the invention causes no environmental pollution. A dyed fabric has soft color and good color fastness, is safe to wear, does not have carcinogenic and teratogenic effects or cause allergic reaction. With good ecological environmental compatibility, the mangosteen shell natural dye is biodegradable.
Owner:ZHEJIANG SCI-TECH UNIV

Eosin staining solution and HE staining solution containing same

The invention provides an eosin staining solution comprising the components of eosin, Biebrich scarlet, flame red, ethanol and water. The invention also provides a preparation method of the eosin staining solution, a HE staining solution containing the eosin staining solution and a staining method of the eosin staining solution. When the eosin staining solution provided by the invention is used for staining slices, the background is clear, the gradation is distinct, the staining effect is good, and the slices can be read easily, cannot fade easily and are convenient to store for a long time.
Owner:无锡市江原实业技贸有限公司

Rapid and sensitive method for detection of biological targets

The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.
Owner:AGILENT TECH INC

Protein and gamma-polyglutamic acid simultaneous coloring counterstaining method

The present invention provides a protein and gamma-polyglutamic acid simultaneous coloring counterstaining method, which is an electrophoresis staining method belonging to the field of biotechnology, and can allow the protein on gel and gamma-polyglutamic acid to be chromogenic simultaneously. The method is characterized by adopting the following steps: (1) preparing protein and gamma-polyglutamic acid electrophoresis samples and performing polyacrylamide gel electrophoresis, (2) fixing the gel after electrophoresis, firstly staining with Coomassie brilliant blue R250 and bleaching, (3) placing the gel after the first staining in a methylene blue dye liquor for re-staining and bleaching until the protein and gamma-polyglutamic acid strips are clear. Compared with the prior art, the present invention solves the problem of simultaneous staining of protein and gamma-polyglutamic acid, can determine molecular weight and purity thereof according to the strip band position and the strip band number of the stained gamma-polyglutamic acid, and is convenient and fast to operate.
Owner:SHANDONG UNIV OF TECH

Imaging system and image processing apparatus

An imaging system that acquires image data through imaging of an object, includes: an imaging unit having an imaging optical system that forms an image of an object, and an imaging device that captures the image of the object formed by the imaging optical system; a stain method estimation unit that estimates a stain method of the object by analyzing image data of the object as obtained from the imaging unit; and a data reduction processing unit that reduces data volume of image data of the object if the stain method estimated by the stain method estimation unit is a predetermined stain method.
Owner:CANON KK

Method and composition for staining and processing a urine sample

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.
Owner:IRIS INT

Immunohistochemical double-molecular-target single staining kit as well as using method and application thereof

InactiveCN110850094ASignificant technical advantagesSignificant clinical valueMaterial analysisChromogenic SubstratesAssay
The invention discloses an immunohistochemical double-molecular-target single staining kit as well as a using method and an application thereof. Based on an expression pattern of a molecular target ina sample to be detected, a cell core type molecular target and a cytoplasm or cell membrane or plasma membrane type molecular target are selected as the to-be-detected double-molecular targets; then,specific to the specific antibody of the double-molecular targets, a kit comprising a mixing primary antibody is formed by mixing, wherein the kit can be used in an immunohistochemical staining method such as an immuno-enzyme linked immunosorbent assay or an immune colloidal gold method; the kit and an enzyme labeled secondary antibody and a chromogenic substrate matched with the kit are adopted,so that synchronous detection of two molecular targets can be completed on one slice through one immunohistochemical staining process. The method is obviously superior to the traditional immunohistochemical and immunohistochemical double staining method, and has the characteristics of low cost and high efficiency, and does not need to increase the additional operation steps.
Owner:XI AN JIAOTONG UNIV

Method and system for digital staining of label-free fluorescence images using deep learning

A deep learning-based digital staining method and system are disclosed that enables the creation of digitally / virtually-stained microscopic images from label or stain-free samples based on autofluorescence images acquired using a fluorescent microscope. The system and method have particular applicability for the creation of digitally / virtually-stained whole slide images (WSIs) of unlabeled / unstained tissue samples that are analyzes by a histopathologist. The methods bypass the standard histochemical staining process, saving time and cost. This method is based on deep learning, and uses, in oneembodiment, a convolutional neural network trained using a generative adversarial network model to transform fluorescence images of an unlabeled sample into an image that is equivalent to the brightfield image of the chemically stained-version of the same sample. This label-free digital staining method eliminates cumbersome and costly histochemical staining procedures and significantly simplifiestissue preparation in pathology and histology fields.
Owner:RGT UNIV OF CALIFORNIA

Gallinaceous visfatin gene 9bp indel polymorphism detection method and application thereof

InactiveCN101805787AOvercoming the limitations of difference detectionOvercome limitationsMicrobiological testing/measurementEnzyme digestionSmall fragment
The invention relates to a gallinaceous visfatin gene 9bp indel polymorphism detection method and application thereof. The detection method comprises the following steps of: designing a primer in the position of a gallinaceous visfatin gene 9bp indel locus; then carrying out PCR amplification; and detecting the polymorphism of the gene 9bp indel by the polyacrylamide gel electrophoresis, wherein the gene is of a DD genotype when 9bp is deleted, the gene is of an II genotype when 9bp is inserted, and the heterozygous individual at the locus is of an ID genotype. The correlation analysis combining the detection results and economic characters of chickens is used for the assistant selection and the molecular breeding of chickens. The detection method does not need be subject to the enzyme digestion, thereby saving the cost and the time; the detection is carried out by adopting the polyacrylamide gel electrophoresis, thereby overcoming the limitation of the agarose electrophoresis to the small-fragment nucleotide difference detection. The method has the advantages of high resolution, high detection sensitivity and accurate genotype judgment; and the gel has on requirements for the staining method, and the ethidium bromide (EB) staining and the silver staining are both applicable.
Owner:HENAN AGRICULTURAL UNIVERSITY +1

Staining method

The invention provides a staining method used for dripping reagents onto tissue slices and performing immunohistochemical staining treatment on tissue slice samples. The staining method is characterized by comprising the following steps of: providing equipment, wherein a machine head is arranged on the equipment, a suction nozzle is sucked onto the machine head, the interior of the suction nozzle is filled with reagents, the machine head drives the suction nozzle to move, and the suction nozzle drips the reagents onto the tissue slices in a manner of describing circle and / or dotting and / or scribing. According to the staining method, the reagent can be added on the tissue slice in a positioning manner, the coverage is wide, and the reagents are saved.
Owner:王素蓉

Staining method of ciliates

The invention discloses a staining method of ciliates, belongs to the technical field of biology, and provides a staining method of ciliates to solve the problems that a traditional ciliate staining method is long in staining time and fussy in staining step, and cytostome and cilium cannot be clearly displayed. A saturated ethyl alcohol mercuric chloride solution is adopted as a fixing agent to carry out pre-fixation on ciliate bodies. In addition, before the pre-fixation operation, to-be-produced ciliate bodies are attached to a to-be-dyed glass slide; a plurality of steps such as dropwise adding protein glycerinum water and collodion are omitted. The staining method disclosed by the invention is convenient to operate; the sensitivity and the staining effect of silver staining of the ciliate protein are greatly improved; and rapid, efficient and accurate display of infraciliature in morphological classification and cytological studies of the ciliates is achieved.
Owner:CHENGDU ACAD OF AGRI & FORESTRY SCI

Staining method for cashmere fibre or cashmere fabric

The invention discloses a staining process for cashmere fiber or cashmere fabric which comprises the steps of, placing the cashmere fiber or cashmere fabric into treatment chamber, lowering the pressure in the treatment chamber to be 10Pa or below, adjusting the pressure in the chamber to be 10-200Pa through non-polymer gas, exerting voltage to the electrode arranged in the processing chamber for low-temperature plasma treatment, and proceeding coloration.
Owner:INST OF CHEM CHINESE ACAD OF SCI

Method for staining superfine polyester fabric by use of FORON CYANIE S-WF as main dye

The invention relates to a method for staining a superfine polyester fabric by use of FORON CYANIE S-WF as a main dye. The FORON CYANIE S-WF dye is also called as FORON BLUE S-RWF and is collectively called as FORON CYANIE S-WF as below. The staining method comprises the procedures of dissolving, dyeing, and post treatment. The method can well solve the problems that quality abnormalities of color spots on the surface of the fabric, surface dyeing and the like can be caused in an existing method for staining the superfine polyester fabric by use of the FORON CYANIE S-WF as the main dye.
Owner:上海嘉麟杰纺织科技有限公司

DNA silver staining method in polyacrylamide gel electrophoresis

The invention discloses a DNA silver staining method in polyacrylamide gel (PAGE) electrophoresis, which comprises the steps of soaking polyacrylamide gel with 0.1% AgNO3 for 10-15min, washing with distilled water twice, developing with 0.04% Na2CO3, 2mL of 37% HCOH / L and 2% mixed solution for 5-6min, and flushing twice with distilled water to complete the whole dyeing process. The conventional DNA silver staining method needs 5-6 chemical reagents, prepares 4 solutions and spends 40-60min to complete the dyeing step, but the method only needs 4 chemical reagents, prepares 2 solutions and spends 20min to reach the result the same as that of the conventional dyeing method, and in denaturing polyacrylamide electrophoresis (PAGE), the dyeing method can reduce the amount of DNA to 0.44ng; and in non-denaturing polyacrylamide electrophoresis (PAGE), the amount of DNA can be reduced to 3.5ng. Compared with the conventional dyeing method, the method of the invention saves the time, reduces the cost, and increases the detection sensitivity.
Owner:NORTHWEST A & F UNIV

Novel method for in vitro culturing and staining Neospora caninum tachyzoites

The invention relates to a novel method for in vitro culturing and staining Neospora caninum tachyzoites, which is characterized in that the concrete culturing steps are as follows: culturing MCF-7 breast cancer cells using a conventional method, inoculating in 1ml Neospora caninum at an inoculum size of 10<4> / ml after the cells grow to form a monolayer of cells, culturing with RPIM-1640 medium containing 2% fetal bovine serum, and observing the number of extracellular Neospora caninum tachyzoites with an inverted microscope everyday; and the concrete staining steps are as follows: fixing Neospora caninum tachyzoites with paraformaldehyde, staining with acridine orange, and observing and taking photos with a common fluorescence microscopy. The culturing method of the invention is simple and practical, and Neospora caninum tachyzoites can be rapidly cultured. In addition, after acridine orange staining, intracellular parasites can be rapidly, visually and clearly observed with the common fluorescence microscopy.
Owner:JILIN UNIV

Staining method for glass fiber cloth and multiple-shaft transmission device thereof

The invention discloses a staining method for glass fiber cloth and a multiple-shaft transmission device thereof. The staining method includes the following steps: the glass fiber cloth is tensioned through the multiple-shaft transmission device, and can move in a certain speed; when the glass fiber cloth moves evenly, the glass fiber cloth is painted in a reciprocating mode by using the method of paint spraying at the same time so that the glass fiber cloth can have needed colors; and after being painted, the glass fiber cloth is moved as well as dried, and is packed into a roll through the multiple-shaft transmission device. By means of the staining method for the glass fiber cloth, continuous production of glass fiber cloth staining is achieved, meanwhile, process is simplified, and the production and packing can be carried out smoothly; and the stained glass fiber cloth can be widely used in composite materials and provide people with good visual feelings and appearance performance.
Owner:苏州福克施乐塑料制品有限公司

Tumor cell detecting method

The invention discloses a tumor cell detecting method, and belongs to the technical field of tumor cytology. The detecting method comprises the following steps of applying alpha fetoprotein with enzyme labeling or alpha fetoprotein heteroplasmon to combine with alpha fetoprotein acceptor heteroplasmon expressed by tumor cell; through the chromogenic reaction between substrate and enzyme, dyeing the tumor cell, and thereby distinguishing the tumor cell and the normal cell. The tumor cell detecting method can specially identify the tumor cell; the method overcomes the shortcomings of the traditional dyeing method, and is applicable to various tissue specimens or cell specimens; therefore, the method has very abroad application prospect.
Owner:HUNAN NEWLAND BIOTECH
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