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Staining kit and preparation method, staining method, and use thereof

A technology of staining reagents and staining solutions, which is applied in biochemical equipment and methods, and microbial measurement/inspection, etc. It can solve the problems of expensive reagents, non-standardized trace immunofluorescence methods, and long time-consuming separation and culture methods, and achieve inspection items. many effects

Inactive Publication Date: 2003-08-06
裴芳君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For non-gonococcal urinary tract infection, the traditional isolation and culture method takes too long, the micro-immunofluorescence method is not very standardized, and the internationally approved SyV2 reagent is expensive, and it is not easy to popularize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Get 50% dehydrated methanol and 50% dehydrated ethanol (volume percentage) after adding in equal amounts as the fixative;

[0035] Put 1.5g of Wright's powder and 1.5g of Giemsa's powder together in a clean mortar, add a small amount of methanol to fully grind, absorb the upper layer, then add a small amount of methanol, continue grinding, and then absorb the upper layer, and so on. Several times, until the internal dye in the mortar is fully dissolved and washed, put it into a brown glass bottle, add 0.6g of Azure II, 100ml of glycerin, and make up methanol to 900ml as a staining solution. Shake well once a day for a total of one week, store for another week, filter, and store in a brown ground glass bottle for later use.

[0036] Take 7 g of anhydrous potassium dihydrogen phosphate, 3 g of anhydrous disodium hydrogen phosphate, add 1000 ml of distilled water as a differentiation solution, and adjust the pH value to pH 6.8 with phosphate.

[0037] The kit consists of ...

Embodiment 2

[0039] Put 6 drops of fixative solution on the cervical smear, fix it for 20 seconds, and pour off the fixative solution by moving the slide sideways. Add 2 drops of staining solution, spread the staining solution over the specimen, and dye for 5 seconds. Immediately add 8 drops of differentiation solution, gently rotate the slide to mix it with the staining solution, and differentiate for 38 seconds. Use small running water to slowly buffer off the residual liquid and its sediment, wipe off the moisture on the back of the slide, and examine the wet slide.

[0040] Note: 1. The mouth of the bottle must be pierced with a needle before use, so that the dye solution can drip out smoothly. 2. When scraping (smearing) slices, the position should be appropriate, the sample should be sufficient, the technique should be light, and the smear should be thin. Too few specimens are often the main reason for missed diagnosis. 3. The dyeing time can be appropriately extended according to...

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PUM

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Abstract

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need oftwo main, and its detection accuracy rate is high, can be up to 98%.

Description

(1) Technical field: [0001] The invention relates to a staining reagent kit, a preparation method thereof, a staining method and the application in rapid multi-pathogen and cytological examination of reproductive tract infections / sexually transmitted diseases. (two) background technology: [0002] The traditional staining methods are Papanicolaou and HE, which are world-renowned staining methods for cytopathology. The fixation time is long, the staining time is long, the staining steps are many, the reporting speed is slow, the staff is large, the number of daily readings is small, and the cost is high. The operation is complicated, labor-intensive, time-consuming and expensive. [0003] Giemsa rapid staining method. Bryon-PA (Patnol Biol 9aris 1996; 24(1): 75-9) once reported that Giemsa rapid staining method was used for bone marrow smear examination. In 1977, Thatcher-RW and others were used for conjunctival cytological examination, and in 1978, Howell-WM (Hum Genet. 19...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 裴芳君
Owner 裴芳君
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