31 results about "Silver Staining Method" patented technology

The invention discloses a polyacrylamide gel (PAG) solution for microsatellite marker (also known as simple sequence repeats or SSR) polymorphism detection. The polyacrylamide gel solution includes a polyacrylamide gel mother liquor, 5*TBE, a 10% APS and TEMED, diluting is carried out by adopting distilled water until the volume of the polyacrylamide gel mother liquor accounts for 21-22% of the total volume, and the amount of the 5*TBE is the same as that of the polyacrylamide gel mother liquor. When the polyacrylamide gel solution is used, PAG has less damage during an oscillation developing process, PAG splicing is avoided, the experimental error is reduced, and the accuracy of results is guaranteed.

The invention relates to a method for dyeing DNA (Deoxyribose Nucleic Acid) on polyacrylamide gel. The method is a safe and environment-friendly method for dyeing acidic silver ion solution. The method sequentially comprises the steps of fixing, primary washing, impregnating, secondary washing, developing, and developing reaction ending. In addition, the invention further relates to a method for detecting the DNA on the polyacrylamide gel, and a kit for the methods.

The invention discloses a method for screening the allelotype of an immune related gene DQA1 Exon 2-(A-C) of goat. A primer is designed according to the sequence of exon-2 of a DQA1 gene, the genome DNA of white goat is used as a template to carry out PCR (Polymerase Chain Reaction) amplification, purification sequencing is carried out on the PCR product, and the polymorphic site of the exon-2 of DQA1 gene is screened preliminarily; a target fragment is subjected to PCR amplification by using DNA of the white goat genome as a template, and the PCR product is treated by a denaturing agent, and the denatured amplification fragment is subjected to polyacrylamidedel gel electrophoresis detection; rubber is dyed by applying a silver staining method, and partial allelotype of the exon-2 of the DQA1 gene of white goat from Guizhou is determined according to the result of polyacrylamidedel gel electrophoresis. A convenient, quick and accurate method is provided to PCR-SSCP screening and type determining of exon-2 polymorphism of DQA1 gene of different varieties of goat, and can be used for detecting partial allele of exon2 of goat immune related DQA1 gene, so that the problem of difficult type determination on highly polymorphic genes in the existing PCR-SSCP technology can be solved.