80results about How to "Effective detection means" patented technology

The invention relates to a micro-torque mechanical testing machine and method. The testing machine comprises a control device and a testing machine host, wherein the control device is used for setting a testing method, sending a command to the testing machine host and analyzing and processing test data obtained from the testing machine host; and the testing machine host is used for loading a test sample. The testing method comprises the following steps of: controlling a signal generating module to generate a voltage signal through customized testing software, and driving the testing machine host to work after conditioning and amplifying; acquiring an angle signal and a coil driving signal by a signal acquisition module; converting the acquired angle signal into a rotation angle, and converting the acquired coil driving signal into a torque; and carrying out data processing according to a mechanical model to obtain a shear stress-shear strain curve of the test sample, thereby further calculating shear modulus and shear strength. By adopting electromagnetic driving and torque measuring ways, the torque resolution of the torque testing machine is greatly improved.

The invention discloses a human body composition analysis method based on a genetic algorithm. The human body composition analysis method based on the genetic algorithm comprises the following steps: eight sections of human body impedance models are selectively used and an expression of each section of human body impedance is analyzed and calculated; groups of different voltage and current are set so that groups of human body impedance data models are obtained through calculation; an AIC value of each group of human body impedance data models is calculated through an akaike information criterion and combination with human body physiological parameters; a fitting model is selected; and genetic evolution is conducted on a position coefficient of the fitting model, an unknown parameter of the fitting model is determined through copy, intersection and mutation operation and a human body composition predicting formula is obtained. According to a calculation method of the eight sections of human body impedance models, theoretical reference can be provided to eight section impedance measurement technology. According to the human body composition predicting method based on the genetic algorithm, human body composition predicting accuracy can be improved and an effective detection measure is offered for human body composition research and clinical application.

The invention discloses a method for detecting the use performance of a refractory material, and the method can predict the use effect of the refractory material. The method comprises the steps of: detecting the ability of the refractory material resisting thermal stress, employing a temperature controllable electric resistance furnace, and reserving grooves for placing samples on the furnace doors; making the to-be-detected refractory material into samples and inlaying the samples into the grooves on the two metal furnace doors; raising two plugboards through an electric lifting device outside the furnace, and closing the front and back metal furnace doors; observing the sample surfaces by alternate cooling and heating, and performing detailed recording; detecting the heat conduction performance and sintering gradient of the refractory material, and inlaying the to-be-detected refractory material into the two metal furnace door grooves; starting an automatic temperature controller, and beginning heating, constant temperature and cooling; and cutting the samples open longitudinally, and utilizing an X-ray diffraction instrument and an electron microscope to determine the mineral composition and microstructure at different distances from a hot surface. The method provided by the invention has the advantages of simple operation and convenient maintenance, and is in favor of large scale popularization and application.

The invention discloses a multiplex fluorescence quantitative PCR detection primer and probe combination and detection method for simultaneously detecting four duck-susceptibility viruses. Sequences of primers include SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQID NO:7, SEQ ID NO:8, SEQ ID NO:10 and SEQ ID NO:11 respectively; sequences of probes include SEQ IDNO:3, SEQ ID NO:6, SEQ ID NO:9 and SEQ ID NO:12 respectively. The four duck-susceptibility viruses include the duck-derived avian influenza virus, the duck-derived newcastle disease virus, the duck hepatitis a virus and the duck tembusu virus. By means of the multiplex fluorescence quantitative PCR detection primer and probe combination and detection method, the four viruses can be detected simultaneously, pathogens can bequantitatively detected, the detection is quick and convenient, and the time and labor are saved.

The invention discloses a protein chip antibody detection kit for an avian infectious bronchitis virus and application thereof. The kit comprises (1) an IBV nsp5 protein chip prepared from a recombinantly expressed IBV non-structural protein 5; (2) an IgY enzyme-labeled antibody solution diluted by using an antibody diluent; (3) a 20*TBST concentrated washing solution; (4) diluent serum; (5) a positive control sample, i.e., anti-IBV nsp5 chicken serum; (6) a negative control sample, i.e., negative SPF chicken serum; (7) chemiluminescent substrate liquid including chemiluminescent liquid A andluminescent substrate liquid B; and (8) a TMB color-developing solution. The IBV protein chip antibody detection kit provided by the invention uses the non-structural protein nsp5 as an antigen, has the characteristic of high coincidence rate with imported commercial kits and is substantially reduced in cost, faster in detection, higher in sensitivity and simple to operate.

The invention discloses a PCV3 epitope polypeptide sequence screening method. The amino acid sequences of comprised five polypeptides are respectively PCV3-1: GTPQNNKPWH; PCV3-2: SPAQQTKTMF; PCV3-3: WLQDDPYAESSTRKV; PCV3-4: MTSKKKHSRY; PCV3-5: VPEKTGMTDFYGTKE. The method uses an ELISA (Enzyme-Linked Immuno Sorbent Assay) method to detect antiserums IgG of PCV2 and PCV3, and an oligopeptide specifically combined with a PCV3 antibody is screened through the ELISA method. The screened oligopeptide provides a reliable evidence for a PCV3 subunit vaccine, a related agent used for diagnosing the infection of a circovirus 3, and an antibody for treating the infection of the circovirus 3, etc. The PCV3 epitope polypeptide sequence screening method has the advantages that the antigenicity of the screened five polypeptides are good, the epitope is identified to be held by the PCV3, the sequence is conservative, whether an animal is infected with the PCV3 is identified effectively by applying theepitope sequence, and an effective detection means is provided for the prevention of the PCV3 in future.

The present invention discloses a Vibrio shilonii multiple virulence factor GeXP rapid detection kit and a detection method thereof. According to the present invention, genome DNA of Vibrio shilonii is extracted and is adopted as a template, 13 pairs of multi-gene PCR primers and a pair of universal primers are adopted to carry out multiplex PCR amplification to obtain multiplex PCR reaction products, and a GenomeLab GeXP genetic analysis system is adopted to carry out detection analysis. With the present invention, the special primers are designed based on the 13 Vibrio shilonii virulence genes, a single-tube reaction is performed to rapidly and efficiently detect the 13 genes in one time so as to obtain the following results, the detection coverage is wide, and the Vibrio shilonii detection accuracy can be substantially improved, wherein the results comprise whether the detected sample contains the virulence carrying gene and the Vibrio shilonii having the potentially pathogenic ability. In addition, a certain theory basis is provided for coral bleaching phenomenon prevention and control, and important practical significances are provided for further coral island protection in our country and further degradation prevention.

The invention relates to a test bench for the runout detection of an electronic water pump. The test bench comprises a rack and a conveyor belt, wherein a tray is arranged on the conveyor belt; a profiling base is fixed on the upper end surface of the tray; a wide-type air claw and a lifting cylinder, the output end of which is connected with the wide-type air claw, are arranged at the left side of the profiling base; arm plates are connected on the wide-type air claw; semi-circular clamping boards are connected at one sides, which are close to the profiling base, of the arm plates; a rubber circular block is arranged above the profiling base and connected with a motor; the motor is connected with a sliding seat vertically sliding on the rack; a displacement sensor is arranged at the lowerside of the sliding seat; a downward pushing cylinder is connected at the upper side of the sliding seat; a distance sensor and a leftward pushing cylinder are arranged at the right front side of thetray; the leftward pushing cylinder is connected with a limit block; and a PLC (Programmable Logic Controller) controller for controlling the operation of the device is arranged on the rack. The testbench disclosed by the invention has the beneficial effects that the online runout detection of an impeller rotor on the electronic water pump is realized through the displacement sensor, so that thelabor intensity is greatly reduced and the work efficiency is improved; and the test bench has the advantages of high detection precision and accuracy.

The invention discloses a rapid detection kit and a detection method for multiple virulence factor GeXP of vibrio coralliilyticus. Directed at 20 known vibrio coralliilyticus virulence genes, specific primers are designed. 20 genes can be detected for one time rapidly, efficiently and simultaneously by using the detection kit, according to the detection method and through the single-tube reaction, and accordingly, whether samples or environments contain the vibrio coralliilyticus carrying virulence relevant genes or having potential pathogenic capacities is detected. According to the rapid detection kit and the detection method for multiple virulence factor GeXP of vibrio coralliilyticus, the detection coverage is wide, the accuracy of vibrio coralliilyticus pathogenicity evaluation can be improved greatly, an effective detecting method is provided for vibrio coralliilyticus pathogenicity evaluation, and a large significance is provided for monitoring and preventing and treating coral bleaching diseases.

The present invention discloses a Vibrio shilonii multiple virulence factor GeXP rapid detection kit and a detection method thereof. According to the present invention, genome DNA of Vibrio shilonii is extracted and is adopted as a template, 13 pairs of multi-gene PCR primers and a pair of universal primers are adopted to carry out multiplex PCR amplification to obtain multiplex PCR reaction products, and a GenomeLab GeXP genetic analysis system is adopted to carry out detection analysis. With the present invention, the special primers are designed based on the 13 Vibrio shilonii virulence genes, a single-tube reaction is performed to rapidly and efficiently detect the 13 genes in one time so as to obtain the following results, the detection coverage is wide, and the Vibrio shilonii detection accuracy can be substantially improved, wherein the results comprise whether the detected sample contains the virulence carrying gene and the Vibrio shilonii having the potentially pathogenic ability. In addition, a certain theory basis is provided for coral bleaching phenomenon prevention and control, and important practical significances are provided for further coral island protection in our country and further degradation prevention.