252 results about "Spermine" patented technology

The invention provides a liposome-modified spermine derivative and a liposome prepared by the derivative. The spermine derivative is directly applied or is mixed with one or more selected from cholesterol, neutral lipids and polyethylene glycol (PEG)-modified lipids, which can be adopted as a carrier for entrapping or absorbing bioactive molecular drugs to be loaded into cells. In this way, the effect of regulation, intervention or treatment is realized. In a general formula (1), X1 represents -(CH2)- or carbonyl group, wherein n represents 1, 2 or 3; X2 represents -(CH2)-, ester group, amide group, oxygen, or sulfur; R1 and R2 independently represent C6-C18 alkyl group or lipophilic cholesterol molecules, respectively.

The invention discloses a human mesenchymal stem cell adipogenesis inducing and differentiating culture medium and a preparation method thereof. The human mesenchymal stem cell adipogenesis inducing and differentiating culture medium is produced by the following components of an alpha-MEM / HG-DMEM culture medium, 5-50% of percent by volume of fetal calf serum, 0.5-10% of percent by volume of glutamine, 100-400 [mu]M volume of indomethacin, insulin with the concentration of 0.1-20 [mu]g / ml, 10-200 [mu]M volume of 1-methyl-3-isobutyl xanthine, 10-200 nM volume of dexamethasone and 0.1-20 [mu]M volume of spermine. The preparation method includes the following steps that a culture dish is cleaned; the culture medium is provided, and the alpha-MEM / HG-DMEM culture medium is prepared in the culture dish according to a formula; materials are mixed; and mixed liquor is filtered. Multiple histologic origin human mesenchymal stem cells including human mesenchymal stem cells, umbilical cord mesenchymal stem cells and adipose tissue-derived stromal cells are induced to the directional differentiation of adipogenesis cells; differentiating and inducing time of the human mesenchymal stem cells isshortened, the preparation method is convenient, and the differentiating and the inducing of human mesenchymal stem cell adipogenesis can be achieved stably and efficiently.

Use of certain castanospermine esters in the treatment of diseases caused by flaviviruses, in particular in the treatment of disease caused by the hepatitis C virus (HCV) and compositions containing said esters in combination with adjunctive therapeutic agents.

The invention discloses a method for improving the mango fruit setting rate, which comprises: preparing a fruit setting solution; and spraying the mango flower with the fruit setting solution during the flowering period and the fruit setting period. The fruit-setting solution is selected from spermine aqueous solution or sodium nitroprusside aqueous solution, or from urea, boric acid, sodium molybdate and magnesium sulfate aqueous solutions. The invention can improve the mango fruit setting rate and the proportion of large fruits, and reduce seedless fruits.

The invention relates to a detection kit for ureaplasma urealyticum (UU) nucleic acid by utilizing RNA constant-temperature amplification. The detection kit comprises a urine sample preserving solution, a nucleic acid extraction solution, a UU reaction solution, a UU detection solution, an SAT (Spermidine / Spermine N1-Acetyltransferase) enzyme solution, a UU positive control and a UU negative control. The detection kit provided by the invention has the analytical sensitivity of 50 CFU / reaction for detecting UU, and has the characteristics of high specificity and high sensitivity; and an amplification product RNA is easy to degrade in a natural environment and causes low pollution.

The invention belongs to the technical field of plant growth conditioning agents, and relates to a plant salt resisting conditioning agent. The plant salt resisting conditioning agent is prepared from porphyra polysaccharides with the concentration of 100-1,000 mg / L, salicylic acid with the concentration of 0 mg / L or 100-200 mg / L, brassinolide with the concentration of 0 mg / L or 0.01-0.1 mg / L, glycine betaine with the concentration of 0 mg / L or 100-1,000 mg / L, spermidine with the concentration of 0 mg / L or 10-100 mg / L, a surfactant with the concentration of 0 mg / L or 1,000-20,000 mg / L and water, wherein the water serves as a solvent, and low-molecular-weight porphyra polysaccharides with the molecular weight of 1 k-50 k Da are adopted as the porphyra polysaccharides. Accordingly, novel application of the porphyra polysaccharides is supplied, and the porphyra polysaccharides can be used for preparing the plant salt resisting conditioning agent; meanwhile, by compounding the porphyra polysaccharides with the biological source induced resisting substances such as salicylic acid, brassinolide, glycine betaine and spermidine to prepare the salt resisting conditioning agent, the comprehensive resistance of plants on salt stress can be improved, and normal growth and development of the plants are guaranteed; according to the salt resisting conditioning agent, the components are simple, the raw materials are easy to obtain, the cost is low, the effect is significant, and environmental friendliness is achieved.

The invention provides a rubber tree embryoid induction culture medium, an induction method for an embryonic callus and a culture method for regenerating resistant callus plant, and belongs to the technical field of plant tissue culture. The rubber tree embryoid induction culture medium takes an improved MS culture medium as a basic culture medium, and is prepared from Kt, NAA, 6-BA, salicylic acid, spermidine, acid hydrolyzed casein, coconut liquid endosperm coconut water, active carbon, saccharose and phytagel. The somatic embryo induction rate of the embryoid induction culture medium provided by the invention for inducing the embryonic callus of a rubber tree anther somatic embryo is increased by 2.04 to 2.16 times in comparison with that of an anther dedifferentiation callus.

Water-based drilling mud lubricants using a blend of fatty acid polyamine salts and fatty acid esters give synergistically better lubricity results than either component used separately. For example, the blends with different ratios of fatty acid diethylenetriamine salt and fatty acid methyl ester demonstrate much better lubricity in water-based drilling fluids than those where only fatty acid diethylene-triamine salt or fatty acid methyl ester are separately used. The amines in fatty acid amine salt might also include other polyamines, such as butanediamine pentamethylenediamine, spermidine, spermine, propylene diamine and propylene polyamines. The fatty acid esters might also include fatty acid ethyl ester, fatty acid glycerol ester and fatty acid trimethylolpropane ester. The carbon numbers of the fatty acids used to make the components in the lubricant blend may range from C4 to C28.

The present invention is directed to a design of and a method to synthesize polycations for gene (DNA and RNA) delivery. According to this design, the polycations (also said cationic polymers) are formed by polymerization of endogenous monomers bearing sufficient amino groups through degradable bonds with linker molecules or with themselves. The amino group-bearing monomers are those naturally existing in or nontoxic to human body. The linker molecules are those which are not only degradable to nontoxic fragments but also able to release the amino group-bearing monomers in their native state upon degradation. Some examples for the endogenous amino group-bearing monomers are spermine, spermidine, serine or N,N-dimethyl serine, and histidine. Examples for the degradable chemical bonds formed between the amino group-bearing monomers are carbamate, imine, amide, carbonate, and ester. In order to improve degradability or proton sponging effect, low pKa (<8) amino group(s) or other electrondonating group(s) is incorporated in the linker between the two (or three) reactive groups for linking the amino group-bearing monomers. These polycationic carrier systems can be used for nano-encapsulation and transfection of gene materials.

The invention relates to a biological detection method, and particularly relates to a diacetyl spermine detection kit and a preparation method thereof. The diacetyl spermine detection kit comprises a test strip, wherein the test strip sequentially comprises a glass cellulose film, a gold pad, an NC film and absorbent paper from a sample charging end. The diacetyl spermine detection kit is characterized in that monoclonal antibodies of DiAcSpm and CRE labeled by colloidal gold and a rabbit antibody are coated on the gold pad; a detection region (T) on the NC film has antigens of the DiAcSpm and the CRE, and a standard region (C) has a goat anti rabbit antibody. According to the diacetyl spermine detection kit, by applying principles of cytobiology, molecular biology, biochemistry, immunology and the like, using a colloidal gold immunochromatography technology, and adopting single methods such as urinalysis and the like which are easy to operate and noninvasive, a diacetyl spermine in vitro diagnosis kit for early discovery and early diagnosis of diacetyl spermine in urine of patients suffering from multiple cancers can be obtained. The detection is performed by using a colloidal gold immunochromatography analyzer, and the purposes of rapidness, accuracy and convenience in operation are achieved.

The invention provides a method for enhancing the root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet. In the production process, MS solid medium is utilized in the production process as the basic medium, and the Grosvener Siraitia single link stems with buds are taken as explants. The method is characterized in that Angustmycin and spermine are added into the MS solid medium, and regenerated plants with complete roots and seedlings, few callus and developed and robust root system can be obtained in the two production processes of starting cultivating of explants and subculture enrichment culture, which can be transplanted out of bottles and can also be propagated and enlarged continuously. Conventional cultivation needs 25 days, the number of roots of each plant is great than or equal to 8, and the process of generating roots in the traditional method can be completely omitted.

The invention relates to a culture solution capable of improving the content of resveratrol in polygonum cuspidatum callus and a culture method. The culture solution is composed of an MS basic culture medium, 6-benaylaminopurine, 6-glycosylaminopurine, naphthylacetic acid, spermine, spermidine and sucrose, wherein the concentration of the 6-benaylaminopurine is 0.5mg / L-1.8mg / L, the concentration of the 6-glycosylaminopurine is 0.3mg / L-1.0mg / L, the concentration of the naphthylacetic acid is 0.15mg / L-0.45mg / L, the concentration of the spermine is 1.0mg / L-4.5mg / L, the concentration of the spermidine is 0.5mg / L-1.25mg / L and the concentration of the sucrose is 20g / L-50g / L; and the pH value of the culture solution is 5-6. When the polygonum cuspidatum callus is cultured by using the culture solution provided by the invention, the content of the resveratrol can reach 0.12%-0.18% of the dry cell weight; and the culture solution has the beneficial effect of improving the content of the resveratrol in the polygonum cuspidatum callus.

The invention discloses cationized mulberry polysaccharide nanoparticle gene vectors, which are gene vectors combining mulberry polysaccharides modified by an amine compound and DNA plasmids, wherein the molecular weight distribution of the mulberry polysaccharides is 3 to 3.5*10<4>Da; and the mass ratio of the cationized mulberry polysaccharidesto the DNA plasmids is (0.5-100):1; the particle size of the plasmids is 50 to 150 nanometers; and the amine compounds is spermine, ethylenediamine or polyethyleneimine with a number-average molecular weight of 600Da to 2,000Da. The three kinds of cationized mulberry polysaccharides used in the invention all have good DNA plasmid bonding actions and can form biodegradable, high-efficiency and low-toxicity novel non-viral gene vector materials. And the preparation processes of the cationized mulberry polysaccharides are simple and the prices of the cationized mulberry polysaccharides are low. The invention discloses a manufacturing method of the cationized mulberry polysaccharide nanoparticle gene vectors.

The invention discloses a bio-polyamines-modified graphene adsorption material for selectively adsorbing phenol in mainstream smoke of cigarettes as well as a preparation method and application of the bio-polyamines-modified graphene adsorption material. The bio-polyamines-modified graphene adsorption material is prepared from the following raw materials in parts by weight: 1 part of graphite powder, 5-50 parts of inorganic acid, 30-100 parts of strong oxidative substance, 5-20 parts of bio-polyamines and 0.3-1 part of 1-ethyl-(3-dimethylaminoprpyl) carbodiimide. The preparation method comprises the following steps: firstly preparing the graphite powder into a crude carboxylated graphite product, then adding polyamines and EDC, and purifying to obtain a spermine modified graphene adsorption material. The spermine modified graphene adsorption material is added into a cigarette with a cellulose acetate filter tip when used. According to the bio-polyamines-modified graphene adsorption material as well as the preparation method and the application thereof, modified graphene modified with a functional polar group is firstly used in the field of reduction of tar and harm of tobaccos, has the adsorption efficiency far higher than the traditional bulk-phase adsorption material and a three-dimensional nanometer adsorption material and is capable of greatly improving the clearing efficiency for harmful substances in the mainstream smoke of the cigarette.

The invention relates to a fat nutrient composition comprising the following raw materials, by weight: 15 to 35 parts of medium-short chain mono-diglycerides, 10 to 15 parts of medium-chain triglycerides, 10 to 20 parts of coconut oil or palm kernel oil, 3 to 10 parts of triglyceride-type fish oil, 10 to 30 parts of OPO structure lipids, 3 to 8 parts of carnitine, 1 to 5 parts of spermine and 40 to 90 parts of encrusting substance. A short-medium-chain mono-diglycerides used in the fat nutritive compositions is characterized by short carbon chains, small molecules and strong polarities, and absorbed by the body rapidly. As a plurality of medium-short chain mono-diglycerides are compounded in a certain proportion, the fat nutrient composition has the advantages that an emulsifying property is good, an antibiotic spectrum is wide, an antibacterial effect is good, an intestinal development is promoted, the intestinal health is maintained, the immunity is improved, and the composition has no side effect. The composition also has a good emulsifying performance so that other fat components can be fully emulsified without adding additional emulsifiers.

The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.

The invention discloses an efficient liquid-phase chromatographic detection method for content of polyamines (putrescine, spermidine and spermine) in a goose tissue. Chromatographic conditions of the method are as follows: a C18 chromatographic column, 5 mu m, 4.6*250mm; a volume ratio of mobile phase methanol: water of 62:38; flow velocity of 1.0 mL / minute; ultraviolet detection wavelength of 229 nm; and a column temperature of 25 DEG C. A tissue solid-phase extracting method comprises the following steps: adding 0.1g of tissue into 1 mL of 5% HClO4 and 10 mu L of an internal standard substance for homogenizing and extracting supernatant; adding 2 mL of 2.5 mol / mL NaOH and 1.4 mu L of benzoyl chloride, carrying out water-bath for 30 minutes at a temperature of 40 DEG C; adjusting pH value to 7.0, then, adding into the C18 column, and flushing by 15 mL of water and 15 mL of 15% methanol; and eluting by 0.5 mu L of methanol. The scheme can effectively separate putrescine, spermidine and spermine in the tissue, and can quickly and accurately detect content of the polyamines in the goose tissue, is simple and convenient to operate, high in accuracy and good in repeatability.

The invention discloses a nanometer carrier for simultaneous delivery of medicines and genes and a preparation method of the nanometer carrier, and particularly relates to a simultaneous-delivery nanometer carrier PLGA-pSPE (polyspermine)-PEG (polyethylene glycol)-Ligand capable of simultaneously loading medicines and genes. The medicine delivery system is realized by the following steps: synthesizing pSPE with spermine at two ends by using spermine and polyethylene glycol acrylate (PEGDA) through Michael addition reaction; generating amphipathic PLGA-pSPE from a pSPE and poly(lactide-co-glycolide) copolymer PLGA through condensation reaction of amino and carboxyl; and generating PLGA-pSPE-PEG-Ligand by using PLGA-pSPE and PEG (HOOC-PEG-Ligand) of which one end is provided with carboxyl and the other end is connected with a target head through condensation reaction of amino and carboxyl, and self-assembling to form nano particles, wherein kernel PLGA is capable of well loading insoluble medicines as a hydrophobic material; the pSPE contains a lot of secondary amino groups, is positively charged, is capable of well loading negatively charged genes and grafting an invisible material PEG with the target head; relatively good target medicine delivery can be realized under the combined action of the PEG and the target head; and the medicines and the genes are delivered to the same target cell.

Disclosed are methods and probiotic compositions for the treatment of a metabolic disease or disorder such as, e.g., obesity, type 2 diabetes, or fatty liver. In some embodiments, heat-inactivated Parabacteroides goldsteinii is enterically administered to a subject, such as a human patient, to treat the metabolic disease or disorder or to promote the development of warm microbiota to treat the metabolic disease or disorder. In some aspects, spermine or spermidine may be administered to a subject or used in vitro to promote the growth of microbiota that can be used for the treatment of a metabolic disease or disorder.

The invention provides a polyamine composite agent enhancing wheat and corn drought resistance. The composite agent is composed of the following components, by weight: 7-280 parts of putrescine, 0.4-10 parts of spermidine, 0.8-16 parts of spermine, 1-15 parts of succinic acid, and 6-150 parts of chitin. According to the invention, the weighed components are dissolved into 50000-150000 parts of water, such that the polyamine composite agent is obtained. Compared with prior arts, formerly commonly used spraying agent components are broken through. While the drought resistance of wheat and corn is improved, polyamine can be absorbed by plants according to agriculturally applied concentrations, such that no residue is left in soil. Even the polyamine absorbed by plants enters human and livestock bodies, the concentrations are greatly lower than those synthesized by human and livestock. The composite agent is harmless to human and livestock, and has a relatively low cost.

The invention relates to biphenyl aromatic hydrocarbon, water-soluble biphenyl aromatic hydrocarbon and a preparation method thereof. The biphenyl aromatic hydrocarbon adopts the structural formula as follows: FORMULA, wherein n is 4-13. According to invention, a thermostatic calorimetric titration experiment (ITC) measures that the bonding constant of a macrocyclic host CBP5 and a spermidine molecule is (1.5 plus or minus 0.2)*10<6>M<-1>. The preparation method is easy to operate and mild in reaction condition, and is suitable for industrial production; the stability of a medicine is improved, so that the medicine is higher in medicinal value.

The invention relates to the technical field of T cell tumor treatment and in particular relates to a compound containing an anti-PD (Program Death)-1 gene and poly-spermidine and application of the compound to treatment of tumors. The compound contains a polyspermidine polymer and the anti-PD-1 gene at the mass ratio of (1 to 100) : 1. The compound is transfected to a T cell in vitro to prepare a self-secretion anti-PD-1 antibody-T cell and can generate a PD-1 antibody, so as to help to block a PD-1 signal and effectively help a depleted T cell, and promote the T cell capable of resisting tumor immune response to treat the tumors. Compared with the prior art, the secretion anti-PD-1 antibody-T cell provided by the invention can be used for effectively treating the tumors, and immune treatment of the tumors can be realized; an in-vivo toxicity problem does not exist.

The invention provides an edible fungus culture substrate, which consists of the following components in parts by weight: 40-50 parts of Chinese medicinal dregs, 20-30 parts of corncob, 20-30 parts of wood chips, 2-4 parts of pulverized lime, 2-3 parts of gypsum powder, 1-2 parts of urea, 1-2 parts of calcium superphosphate, 2-4 parts of plant ash and 0.04-0.1 part of edible fungus three-dimensional nutrient spermine. The edible fungus culture substrate has the beneficial effects: the yield of edible fungi cultured with the culture substrate is equivalent to that of edible fungi cultured with a substrate in which pure cotton shell is taken as a major material, the cost is reduced, and a good economic benefit is achieved.

The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.

The invention, relating to the technical field of medicine, relates to a method for detecting the activity of spermidine / spermine N1-acetyltransferase, that is, a quantitative technique of analyzing SSAT activity through detecting acetylated metabolites of the SSAT substrate. According to the invention, the N-acetylation is regulated mainly through or only through SSAT in SSAT pathway and the specific substrate in the N-acetylation regulation is L-dopa, partial metabolism of the substrate can generate an acetylated metabolite N-acetyl-L-dopa thought the introduction of SSAT, the SSAT upregulation is positively correlated with cancer, accordingly, L-dopa can be used as a cancer mark regulated by the SSAT upregulation, and a novel detection method is provided for early detection, early diagnosis, early treatment and the like of tumors. The method has the advantages of safely, efficiency, strong practicality, convenient and fast operating method, simple usage, high positive incidence, and remarkable effect, and can be used as an auxiliary means for preventing, diagnosing, detecting, protecting, treating and studying various tumors.

The invention provides new lipopolyamines of spermine type of the general formula I, wherein X is C—N bond or aminopolyethyleneglycolcarboxamide linker or o-hydroxy-alkylcarboxamide linker or ω-hydroxyalkylcarboxamidopolyethyleneglycol-carboxamide linker, and wherein a hydrophobic domain Y is an acyl symmetrically branched in the position C(2) or cholesteryl. The invention further provides a method of preparation of said lipopolyamines and their use for construction of polycationic liposomal drug carriers.

The embodiment of the invention discloses sanbao sea cucumber wine and a preparation method thereof, which belong to the technical field of health care food. The sanbao sea cucumber wine comprises the following materials in percentage by mass: 10 to 20 percent of sea cucumber nutrient fluid, 2 to 10 percent of seal penis nutrient fluid, 1 to 5 percent of cobia leaching liquor, and the balance of wine; and the sea cucumber nutrient fluid, the seal penis nutrient fluid and the cobia leaching liquor are mixed evenly and then are added into the wine to obtain the sanbao sea cucumber wine. Sea cucumber, seal penis, cobia are matched with medicinal and edible Chinese medicinal herbs, and the mixture is added into the wine to ensure that the wine contains a plurality of nourishing substances such as mucopolysaeccharide from the sea cucumber, saponin, protein, amino acid, trace elements and the like; and nutrient components such as yang-strengthening factor components in the cobia, spermine in the seal penis, unsaturated fatty acid (OMEGA-3), collagen, hemoglobin, renal active factors and the like ensure that prepared health care wine has the functions of nutrition, nourishment and healthadjustment, and has good nutrient value and health care function.

The invention relates to an SBS (styrene butadiene styrene) composite material, in particular to an anti-aging sisal fiber modified SBS composite material, which is characterized by being prepared from the following ingredients in parts by weight: 50 to 70 parts of SBS, 6 to 13 parts of polystyrene, 5 to 10 parts of modified sisal fiber, 2 to 5 parts of ceramic fiber, 0.01 to 0.05 part of spermine-NO, 0.01 to 0.03 part of citric acid, 0.1 to 0.3 part of dioxane, 1 to 3 parts of antioxidants and 0.5 to 1 part of lubricating agents. The invention also provides a preparation method of the SBS composite material. The SBS composite material prepared by the method has the characteristics of excellent anti-aging performance, excellent anti-impact performance and the like.

The invention relates to poly spermine cations, a construction method thereof, and a preparation method of nano-grade particles containing the poly cations. The invention specifically relates to a type of degradable amide poly spermine cations used for conveying nucleic acid medicaments (DNA and RNA), a construction method thereof, and a preparation method of nano-grade particles. The poly spermine cations provided by the invention have a structural formula represented below, wherein n is 10 to 1000. Compared with prior arts, the poly spermine cations provided by the invention can be used for agglomerating and conveying nucleic acid medicaments. During a process of internal circulation, both the requirements on the stability before the cations reach the target cells and the biological response after the cations enter the target cells are satisfied. Further, the poly spermine cations can independently accomplish non-toxic metabolism in vivo.