34 results about "Oncidium" patented technology

The invention discloses a culture medium for tissue culture of pedicel buds of oncidium hybridum and a tissue cultured seedling propagating method. The culture medium is mainly characterized in that: the combination of concentrations of macroelement components in the culture medium does not exist in the conventional culture medium for the tissue culture of the oncidium hybridum. The method for propagating tissue cultured seedlings of the oncidium hybridum comprises the following steps: utilizing the culture medium to induce the pedicel buds to generate callus and differentiation of protocorm; after the protocorm sprouts and tissue cultured seedlings provided with fake corms are formed, transferring to a rooting medium, and inducing to root; transplanting the rooted tissue cultured seedlings into the medium by a conventional tissue cultured seedling transplanting method. By adopting the technical scheme provided by the invention, the pedicel buds of the hybrid variety of the oncidium hybridum are taken as explants, young plants of the oncidium hybridum are quickly propagated in a way of differentiating the protocorm, and the speed of propagating the young plants of the oncidium hybridum is improved.

Disclosed is the high quality germchit rapid breeding method of Oncidium Luridum and culture medium used thereby, wherein a plurality of Oncidium Luridum original breed and hybrid tissue culture breeding methods and unique culture medium employed are provided. The produced Oncidium Luridum germchit has the advantages of high genetic stability, quick emergence of seedlings, strong seedlings, good seedling quality, strong resistance, and good accretion status.

The invention provides an anther-specific expression promoter in plant, wherein said promoter is a promoter of Oncidium aureusidin synthase gene OgAS1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that comprised a promoter having a DNA sequence as SEQ ID No: 3, and a polynucleotide that encode an open reading frame and is linked to the 3′ end of said promoter, wherein said promoter can activate the transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides also a gene expression vector that contains a promoter having DNA sequence as SEQ ID No: 3. The invention provides further a process for producing a transgenic plant or part of organ, tissue or cell of said transgenic plant containing the above-described gene expression cassette.

The invention provides an Oncidium EST-SSR labeling primer and application thereof. The primer comprises a primer pair as follows: 5'-ATCGTAATCCTGAAGCGTATC-3' and 5'-AAGCCCAAACTATTCCATT-3'. The application of the Oncidium EST-SSR labeling primer comprises the steps of carrying out PCR amplification by taking a DNA sample extracted from Oncidium plants as a template, and labeling Oncidium molecules, wherein a reaction system includes the following components by volume (20mu L in all): 11.3mu L of ddH2O, 2mu L of 10*Buffer, 2mu L of 2.5mmol.L<-1> dNTPs, 1 microliter of each of 10mu mol.L<-1> primers in the primer pair, 0.2mu L of 5U.mu L<-1> Taq polymerase and 2.5mu L of 20ng / mu L template DNA.

The invention discloses a method for improving proliferation and differentiation of protocorm of Oncidium by utilizing concentrated coconut juice, which comprises the following steps: concentrating coconut juice on a rotary evaporator to obtain the concentrated coconut juice for later use; sterilizing shoot tip of an Oncidium plant, and inoculating the sterilized shoot tip to an induction medium added with the concentrated coconut juice for induction culture to generate the protocorm; transferring the protocorm to an enrichment medium for enrichment culture, and then transferring to a differential medium for differential culture to generate cluster buds; and inoculating the cluster buds to a rooting and sprouting medium and culturing the cluster buds into seedlings. The concentrated coconut juice serving as an organic additive can obviously improve the proliferation rate of the protocorm tissue of the Oncidium; and on the basis of improving the proliferation rate of the protocorm tissue, the proliferation rate of tissue culture plantlets is effectively improved; therefore, the invention provides an effective path for industrial production of the Oncidium, and has the advantages of strong practicability, simple operation and good popularization.

The invention discloses a tissue culture propagation method for butterfly oncidium (Pschopsis) seedlings, aiming to provide a method for fast propagation, which can obtain a large number of test-tube seedlings in a short period of time, does not cause damage to the mother plant, and can maintain the excellent quality of the mother plant. Tissue culture propagation methods for traits of Phalaenopsis oncidium. The method comprises the steps of selecting explants with sectioned flower stems of excellent strains of Oncidium phalaenopsis with vigorous growth, sterilizing the explants, inducing adventitious buds in the pedicel seedling induction medium, and then removing the pedicel seedling stem tip Insert the protocorm induction and proliferation medium to carry out the induction and subculture of the protocorm, then carry out the differentiation of the protocorm on the protocorm differentiation medium, and then inoculate the seedlings that can be differentiated from the protocorm on the strong seedling medium for cultivation, When the test-tube seedlings grow to 3-4 cm high, harden the seedlings under natural light in the greenhouse for 7-14 days, take out the test-tube seedlings, wash off the root medium, and plant them in the mixed substrate of bark, blue stone and peat, and further Cultivated into seedlings, the survival rate of transplanting is 90-98%. The invention opens up a new way for the breeding of oncidium phalaenopsis seedlings, and can provide a large amount of oncidium phalaenopsis seedlings for the market.

The invention discloses a method for quickly detecting Impatiens necrotic spot virus on oncidiums. The method uses Impatiens necrotic spot virus antiserum to detect the Impatiens necrotic spot virus on oncidium leaves which represent a concentric circle chlorosis spot symptom; a special primer ZI2R of the Impatiens necrotic spot virus is utilized to synthesize cDNA; the synthesized cDNA is applied, and a special primer ZI2F / ZI2R and a special primer ZI1F / ZI1R are respectively used to amplify to establish a RT-PCR assay. The method can also perform secondary amplification to a PCR product of ZI1F / ZI1R amplification respectively with ZI1F / ZI3R, ZI3F / ZI1R and ZI3F / ZI3R as inner primers to establish a nested PCR assay. The method has high sensitivity and good specificity, and provides an effective detection means for timely preventing and curing the attacking of the Impatiens necrotic spot virus to strains.

The invention relates to a method for rapid propagation of high-quality seedlings of oncidium and the used culture medium. Oncidium, also known as Dancing Girl Orchid, King Phalaenopsis, Tubal Orchid, etc., is one of the largest genus in the orchidaceae. Oncidium has bright colors, large numbers of flowers, and large changes in flower shapes. Some varieties also have a delicate fragrance. It is one of the most widely cultivated and popular orchid species in the world. Many oncidium varieties have been introduced in mainland China in recent years, but the production of seedlings mainly depends on ramets, the propagation speed is slow, and the diseases of the female parent are also easy to infect the seedlings. The invention provides a variety of oncidium original species and hybrid tissue culture propagation methods and the used unique culture medium. The produced oncidium seedlings have the advantages of high genetic stability, fast emergence, strong seedlings, good seedling quality, strong resistance, and good growth. High-quality oncidium seedlings have been produced, and the market is very promising.

The invention relates to a method for improving tissue culture seedling transplanting survival rate of oncidium, and belongs to the technical field of agricultural biology. Through selection of a planting matrix, hardening-seedling, seedling washing, fixed planting and other ways, the problems of difficulty in root growing, heavy disease in hardening-seedling and transplanting stages, low survivalrate after taking out of a bottle, weak stress resistance and others of the tissue culture seedling of oncidium can be solved; the method for improving tissue culture seedling transplanting survivalrate of oncidium can improve the transplanting survival rate; the growth recovery of the tissue culture seedling is quick; the disinfectant applied to the method is reasonable in formula, the air-drying time is suitable; the epidemic disease and soft rot in seedling stage of the oncidium can be reduced; moreover, the method is also good for root growth.

The invention provides a method of improving disease resistance by transforming arethusa with longan ferredoxin gene and belongs to the technical field of genetic engineering. The longan ferredoxin gene (Dlfd3) (Register number: JF733784) is guided to arethusa oncidium by means of a transgenic technology, and the obtained transformed plant represents higher transplanting survival rate and disease resisting capability. Compared with wild plants, the soft rot resistant capability of the transgenic plant is improved from high touch soft rot (Di=100) to intermediate soft rot resistance (Di=27.5). The transplanting survival rate is improved from 79.3% to 95.7%, thereby realizing extremely significant difference.

The invention provides an oncidium protoplast dissociation and culture method which comprises the following steps: taking oncidium protocorm-like bodies as starting materials, carrying out callus induction culture and 4-5 times of subculture to obtain vigorously growing calluses with loose structures for dissociation of protoplasts; carrying out cold treatment on calluses at 4 DEG C for 1 d, putting the calluses into a mixed enzyme solution, carrying out shaking table oscillation enzymolysis for 8-10 h, and purifying protoplasts by using an interface method to obtain a large amount of oncidiumprotoplasts with high activity; continuously using the culture medium for solid-liquid double-layer culture, so that the obtained oncidium protoplast cells can normally grow and continuously split to form small cell clusters, the maximum splitting frequency reaches 10.16%, and the maximum plate planting rate reaches 4.03%. The invention provides a scientific basis for cell fusion, somatic cell hybridization, genetic transformation and germplasm innovation by utilizing oncidium protoplast.

The invention provides a matrix for increasing the survival rate of hardened oncidium tissue culture seedlings. The matrix comprises peat soil, fine bark, vermiculite, carbendazim, rooting and seedling promoter and organic nutrients, the peat soil, bark, vermiculite, carbendazim, rooting and seedling promoter and organic nutrients are mixed into a mixture, the size ratio of the peat soil to the fine bark to carbendazim is 5:1:1, the weight of carbendazim accounts for 0.1%-0.25% of that of the mixture, the weight of the rooting and seedling promoter accounts for 0.1% of that of the mixture, theweight of the organic nutrients accounts for 0.02% of that of the mixture, and the organic nutrients comprise 500-1,000 mg / L of inositol, 0.5-1 mg / L of vitamin B1, 0.1-1 mg / L of vitamin B6, 2 mg / L ofglucine and 0.5-5 mg / L of niacin.

The invention discloses a seedling culture method of oncidium, comprising the following steps: selecting a seedling: selecting an oncidium corm which is health in growth, has been normal flowering and in an ageing state; cutting stems: disinfecting a cutting tool, cutting bud tissue of the oncidium corm; soaking: putting the cut bud tissue of the oncidium corm into a bactericide for soaking; dewatering: fishing out the soaked bud tissue of the oncidium corm in the bactericide, and air drying and dewatering in natural conditions; planting: planting the dewatered bud tissue of the oncidium corm. The seedling culture method has the advantages of high reproduction speed, low aberration rate, short culture cycle, high survival rate and the like, the operation method is simple, the investment cost is low, and market promotion is facilitated.

The invention provides an anther-specific expression promoter in plant, wherein said promoter is a promoter of Oncidium aureusidin synthase gene OgAS1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that comprised a promoter having a DNA sequence as SEQ ID No: 3, and a polynucleotide that encode an open reading frame and is linked to the 3′ end of said promoter, wherein said promoter can activate the transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides also a gene expression vector that contains a promoter having DNA sequence as SEQ ID No: 3. The invention provides further a process for producing a transgenic plant or part of organ, tissue or cell of said transgenic plant containing the above-described gene expression cassette.

The invention relates to a cut flower grading operation method and apparatus thereof for oncidium. The grading operation method comprises putting a cut flower on an operation platform, base part of aflower stalk being perpendicular to a grading, determining which grading reference line the top of the cut flower reaches, and observing other grading indexes, wherein the grading standard line indicating grade is reached if the other grading indexes are achieved, or else, grading is performed according to the other grading indexes; binging each ten cut flowers of the same grade, bundling with color tapes identical with the grade mark color, and packing the bundled cut flowers for postprocess. The cut flower grading operation apparatus thereof for oncidium comprises an operation table, a storing drawer, a table-top grading reference line and four grading standard lines. The invention aims to provide an excellent operation method and an operation apparatus for the cut flower grading work ofoncidium, which enables the cut flower grading work simple, efficient and strong operability.

The invention discloses a novel cultivation method for oncidium hybridum, and relates to the technical field of plant cultivation methods. The method comprises two steps including preparing a cultivation substrate and using the cultivation substrate, wherein the preparation of the cultivation substrate comprises the following steps: preparing ceramsite, treating pine barks, pretreating sand graveland proportioning the substrate components; the ceramsite is prepared according to the following steps: pretreating a ceramsite raw material, treating humus soil, producing semi-finished ceramsite, performing graded drying, performing sintering and performing cooling; and the cultivation substrate is used according to the following steps: putting the prepared cultivation substrate into a flowerpot, wherein the top surface of the flowerpot is provided with a drip irrigation device, and performing drip irrigation by using nitrogen, phosphorus and potassium fertilizer water suitable for the oncidium hybridum, wherein the bottom edge of the flowerpot is provided with a water diversion canal. The method provided by the invention can increase the fertility of the cultivation substrate and prolong the service life, and can also have the effects of saving energy and protecting environments; the use amount of the pine barks are reduced, and the characteristics that the ceramsite has good waterabsorption effects, large spacing and good ventilation effects are utilized, so that the planted oncidium hybridum is more vigorous; and phenomena such as root rotting are reduced.

The invention relates to a postharvest pretreatment method and a postharvest pretreatment device for fresh orchidaceae cutflowers. The method comprises the following steps: binding the collected cutflowers in a orchidaceae culture greenhouse, wrapping the cutflowers with screen cloth; placing the cutflowers on a flower shelf with a pretreative solution; pushing the cutflowers to a disinfection chamber for disinfection; pushing the disinfected flower shelf from the disinfection chamber to a progressive operation chamber for progressive packaging; and opening a drain pipe on the flower shelf to drain the pretreative solution in a sink. The postharvest pretreatment device comprises a main supporting frame, a hinge-folding cutflower layout frame, pretreative solution sinks, drain pipelines, pulleys, and the like; the flower shelf comprises four layers; the folding degree of each layer of flower shelf can be adjusted by hinges according to the length and the amount of the cutflowers; and each layer is provided with the pretreative solution sink and the drain pipeline. Compared with the prior postharvest pretreatment method, the invention is simple and practical, has simple equipment and low manufacture cost and can effectively decrease the production cost; and particularly, the invention can save great space for laying out the cutflowers, greatly saves the consumption of the pretreative solution and effectively improves the preservation effect of the orchidaceae cutflowers for large-scale cutflower production enterprises.

The invention relates to the field of flower cultivation and seedling propagation, and particularly discloses a method for pollination and seed cultivation of fan-shaped oncidium under sterile conditions. The method for pollination and seed cultivation of the fan-shaped oncidium comprises the following steps that after blooming, fan-shaped oncidium flowers are subjected to artificial pollination under sterile conditions; after pollination, the fan-shaped oncidium flowers are transferred into a first culture medium for culture; after ovaries of the fan-shaped oncidium are expanded, the fan-shaped oncidium flowers are transferred to a second culture medium for continuous culture; and a large number of seeds are obtained after fruits are mature. According to the method, mature seeds with high embryo rate and high germination rate are obtained through artificial pollination and fruit development process regulation and control in the culture mediums under sterile conditions, the obtained mature seeds can be used for scientific research and seedling production, and the method has an extremely remarkable promotion effect on fan-shaped oncidium seedling production.

The invention belongs to the field of orchid seedling culture, and particularly relates to a substrate applicable to tissue culture of oncidium. The substrate comprises the following components: bitter almond, flos sophorae, hawthorn, lindernia procumbens, lambert raspberry leaves, lilac daphne flower bud, jasminum giraldii diels, delonix regia, avocado, oat and millet; the substrate added to a culture medium is able to promote the differentiating and the growing of the avocado; the shoot tip starts to germinate after 7 days of grafting during primary culture; clumpy bud points grow from about 50% of explants after 14 days; 3 to 5 strong clumpy buds grow around 86% of explants after 40 days. After the substrate is added to the culture medium, the biochemical components change, and the quality of the cultured seedlings of oncidium is high; the cultured seedlings can grow quickly; the demands of the market on oncidium can be met.

The invention provides a primer combination for detecting 2 soft rot pathogens of orchids, and a detection method. Six primers are designed with a common specific fragment of the two pathogens and canbe used for detection of an Erwinia carotovora subsp.carotovora pathogen and a Dickeyadadantii pathogen of the orchids. Six primers are designed aiming at three common characteristic loci of the twopathogens, so that two positive segments can be generated by a true positive sample and used for elimination of false positivity; and design for eliminating false genitive primers is added, wherein one positive segment can be generated by true negativity through design of loci for two common characteristic loci aiming at oncidium, dendrobium and phalaenopsis. The primer combination and the detection method have low requirements for equipment and a whole course can be completed within only 2 h at the soonest.

The invention provides a plant shape control PP333 preparation for oncidium as well as a preparation method and a control method of the preparation. Every 1,000 ml of the PP333 preparation is preparedfrom raw materials as follows: 1.0-1.5 g of PP333, 0.5-1.5 g of C12H25NaO4S, 6-9 g of polysorbate-80 or polysorbate-40, 60-90 ml of absolute ethyl alcohol and the balance distilled water through heating and dissolution at the temperature of 70-80 DEG C. A plant shape control method comprises steps as follows: one plant with a full pseudobulb and one new bud is selected, and when the new bud growsto unfold a blade to expose the pseudobulb, the PP333 preparation is sprayed for the first time; when a new bud grows to 8-15 cm, the PP333 preparation is sprayed for the second time; when a newly growing bud unfolds blades and expose the pseudobulb, the PP333 preparation is sprayed for the third time, and spraying is performed three times in total. The PP333 preparation has good adhesion, the plant adsorption effect is good, the control and treatment time is easy to master, the spraying method is simple, the treated oncidium has upright and green leaves, full and mellow pseudobulb, upright flowers and branches, plump flowers and compact plant shape, the ornamental value of the potted flowers is greatly increased, and the preparation can be applied to potted flower culture of oncidium ona large scale.

The invention discloses a composition for controlling phytophthora root rot of oncidium. The active pharmaceutical ingredients of the composition consist of mandipropamid, cymoxanil and methyl disulfide according to the weight ratio of 1:(5-10):(0.1-0.5). The composition has a favorable control effect on phytophthora root rot of oncidium.

The invention discloses a method for improving the proliferation and differentiation of oncidium protocorms by using concentrated coconut water. The coconut water is placed on a rotary evaporator for concentration to obtain concentrated coconut water for subsequent use; Inoculate the protocorms produced by inducing culture on the induction medium added with concentrated coconut water after the bacteria, transfer the protocorms to the proliferation medium for proliferation culture, and then transfer to the differentiation medium for differentiation culture to produce clustered buds. The clustered shoots were inoculated on the medium for rooting and strong seedlings and cultivated until they became seedlings. The present invention uses concentrated coconut water as an organic additive to significantly increase the proliferation rate of oncidium protocorm tissue, and on the basis of increasing the proliferation rate of protocorm tissue, effectively improves the reproduction rate of tissue-cultured seedlings, providing a method for industrial production of oncidium Provide an effective way, strong practicability, simple operation, good popularization.

The invention discloses a method for obtaining oncidium and related intergeneric filial generation thereof by using immature embryo in-vitro rescue. Comprising the steps of pollination and embryo rescue. According to the method, an oncidium material combination which cannot obtain a hybrid due to abortion of an immature embryo is used as a parent, after cross pollination, the immature embryo is taken out before abortion for immature embryo rescue culture, so that the immature embryo develops into a seedling, and finally a filial generation of the oncidium and a related genus is obtained. The problem that the hybrid F1 generation cannot be obtained due to immature embryo abortion of oncidium and related intergeneric hybridization thereof is solved, a favorable technical guarantee is provided for development of breeding work of oncidium, and the method has positive significance in germplasm resource innovation of oncidium.

The invention discloses a method for in-vitro preservation and growth recovery after preservation of oncidium germplasm resources. The method comprises the following steps: peeling stem tips from newly grown false bulbs of oncidium, putting the stem tips onto the surface of a protocorm induction medium, culturing so as to obtain protocorm, and performing repeated cutting and subculture on the protocorm so as to breed protocorm of an expected number; culturing by using an in-vitro preservation medium; after the protocorm enters into a development and growth period, performing dark culture at 0-5 DEG C; transferring the dark cultured protocorm onto a differential medium, and performing differentiation on the protocorm, thereby generating seedlings. By adopting an oncidium stem tip culture technique, the protocorm is induced, the protocorm is further differentiated, and thus oncidium seedlings can be generated. The protocorm is preserved at low temperature, and with a proper amount of paclobutrazol, the preservation time can be greatly prolonged, the subculture times can be reduced, and the hereditary characters of the germplasm resources can be effectively maintained. After being preserved for a certain time, the protocorm can be recovered to grow rapidly when being cultured under normal temperature and lighting conditions, and seedlings can be differentiated.

The present invention provides a paclobutrazol preparation for regulating oncidium plant type and its preparation method and regulating method. The paclobutrazol preparation includes the following raw material formula: 1.0-1.5g PP in 1000ml preparation 333 , 0.5~1.5g C 12 h 25 NaO 4 S. 6-9g of polysorbate-80 or polysorbate-40, 60-90ml of absolute ethanol, and the rest in distilled water, prepared by heating and dissolving at 70-80°C. The plant type control method is as follows: select a plant with a full pseudobulb and a new bud, and spray PP when the new bud grows until the leaves unfold to reveal the pseudobulb 333 For the first time of preparation, when new shoots grow to 8-15cm again, spray PP 333 For the second time of the preparation, spray PP when the new sprout leaves grow again to reveal the pseudobulb 333 The preparation is the 3rd time, sprayed 3 times in total. PP of the present invention 333 The preparation has good adhesion, good plant absorption effect, easy control and control timing, and simple spraying method. After treatment, the oncidium leaves are straight and green, the pseudobulbs are full and round, the flower branches are erect, the flowers are plump, the plant type is compact, and the ornamental value of potted flowers is greatly improved. , and can be applied to oncidium potted cultivation on a large scale.

The invention relates to a chemical disinfection tissue culture method of oncidium. The oncidium chemically sterilized tissue culture method includes culture vessel disinfection, medium preparation, induction culture, proliferation culture, rooting culture and bottle seedling transplantation. The oncidium chemical disinfection tissue culture method of the present invention uses chemical reagents to achieve the purpose of sterilization or bacteriostasis. In the process of preparing the culture medium, the culture container and the culture medium do not need to be sterilized by high temperature and high pressure, which reduces the workload and energy. consumption, which simplifies the oncidium tissue culture link; and the operation is simple, as long as it is prepared according to different medium formulas; it is practical and popular, and can effectively reduce the cost of oncidium tissue culture, generally can reduce the cost More than 10%.

The invention discloses a biological purification method for living body oncidium struma. The method comprises the first step of mixing corn, chlorella and vitamin C into mixed fodder according the mass ratio of 8:1-2.5:0.02-0.05, the step of paving the bottom of a temporary rearing box with composite soil mixed with wooden fish stones and injecting an appropriate amount of seawater with the salinity no more than 15%o,placing the collected living body oncidium struma into the temporary rearing box, throwing the fodder accounting for 3-8% of the total weight of the living body oncidium struma every day, temporally rearing the living body oncidium struma for 3-7 days and purifying the living body oncidium struma, and the third step of taking the oncidium struma out after purification and washing the oncidium struma with clear water 3-5 times to obtain the purified living body oncidium struma. The living body oncidium struma is temporally reared through the bottom mud with the wooden fish stones and the fodder formed by mixing the corn, chlorella and vitamin C, harmful heavy metal exceeding the standard can be removed successfully, the nutrition value of the oncidium struma can be improved, and reliable basic materials are provided for finish processing of the oncidium struma.