The invention discloses a mobilis bacillus and application thereof. The Bacillus mobilis is named Bacillus mobilis GZU‑Bac01 (Bacillus mobilis GZU‑Bac01), and was deposited in the China Center for Type Culture Collection on December 7, 2020, with the preservation number CCTCC NO: M 2020863. Bacillus mobilis GZU-Bac01 of the present invention has the nutrient element that can dissociate and release solidification, as: nitrogen, phosphorus, potassium, calcium etc., and dissociation effect is good, and cost is low, and environmental protection, adopts microbial bacterium (Bacillus mobilis GZU‑Bac01) method for dissociating coal gangue to prepare microbial fertilizers, which not only converts insoluble and / or insoluble nutrients in coal gangue into soluble components, but also releases the soil after it is applied to the soil The solidified nitrogen, phosphorus, potassium, calcium, etc. in the plant can be transformed into nutrients directly absorbed and utilized by plants. At the same time, GZU‑Bac01 can also effectively release insoluble phosphorus and / or insoluble phosphorus in other phosphorus-containing minerals. phosphorus.