Cell mass digestion and dissociation promoting liquid and cell counting method

Abstract

The present application provides a cell mass digestion and dissociation promoting liquid and a cell counting method. The cell mass digestion and dissociation promoting liquid comprises the following raw materials in percentage by mass: 1%-5% of polyethylene glycol octylphenol ether; 0.1%-4% of alkyl glycoside; 0.1%-1% of non-surface active agent type sulphobetaine; 0.1%-1% of zwitterionic detergent; 1%-10% of pH regulator; and the balance water. The cell digestion and dissociation promoting liquid can realize digestion and dissociation of cell masses by destroying lipid bilayers of cells, dissolving cytoplasm and cell membranes and destroying weak intermolecular binding bonds, and has high dissociation efficiency on the cell masses, so that the cell counting method provided by the present application can realize rapid and low-cost determination of the number of cells cultured on a support material in a bioreactor.

Description

technical field [0001] The present application relates to the technical field of cell culture, in particular to a dissociation solution for promoting cell mass digestion and a cell counting method. Background technique [0002] The bioreactor is the key equipment for cell culture, which can realize the large-scale culture of cells and their subsidiary biological products. The large-scale culture of adherent cells needs to rely on cell culture scaffold materials to realize the colonization of cells in the reactor. With the increase of the culture days, the cells can achieve large-scale high-density culture in the reactor, and form a compact cell cluster-like three-dimensional tissue-like structure on the scaffold. [0003] At present, general-purpose digestive solutions such as trypsin and collagenase on the market are not only expensive, but also need to rely on cell culture incubators or other constant temperature equipment to achieve the optimal temperature of 37°C for enz...

AI Technical Summary

Problems solved by technology

[0003] At present, general-purpose digestive solutions such as trypsin and collagenase on the market are not only expensive, but also need to rely on cell culture incubators or other constant temperature equipment to achieve the optimal temperature of 37°C for enzyme activity, and it usually takes 20-40 minutes to achieve the optimal temperature. Complete digestion and dissociation of cell pellets

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