338 results about "Cell mass" patented technology

A wastewater treatment system is provided including an aerobic membrane bioreactor and an anaerobic digester system connected to receive wasted solids continuously from the aerobic membrane bioreactor and also connected to return effluent from the anaerobic digester system continuously to the aerobic membrane bioreactor. Further, a process is provided for treating wastewater including the step of wasting a volume fraction of organic cell mass from an aerobic membrane bioreactor to an anaerobic digester system and maintaining a solids retention time (SRT) in the bioreactor that is (1) greater than a time needed to achieve growth of organisms suitable for converting carbonaceous biochemical oxygen demand (CBOD) into cell mass and (2) less than a time at which substantial decay of the organisms occurs. The system and process may further include optional pretreatment and / or phosphorus and / or nitrogen removal downstream of the membrane bioreactor system.

A method for treating subjects with abnormal cell proliferation is provided. The method involves administering to subjects in need of such treatment an effective amount of an agent of Formula I, to inhibit cell proliferation such as that associated with tumor growth and metastasis. A method for inhibiting angiogenesis in an abnormal proliferative cell mass by the administration of an agent of Formula I is also provided.

The invention discloses a preservation solution for mesenchymal stem cells and application thereof. The cell preservation solution contains human albumin and heparin as the main components, and other auxiliary reagents such as human cytokine, phosphate ion, metal ions or monosaccharide are contained in a buffer solution for preserving human mesenchymal stem cells. The preservation solution can keep high survival rate of human mesenchymal stem cells in transportation process, reduce adhesion between cells and between the cell and the inner wall of a container, and reduce the possible occurrence of cell mass embolism in blood vessel while clinically infusing human mesenchymal stem cells. The mesenchymal stem cells can be maintained in a state of single-cell suspension at an environment temperature of 4 to 15 DEG C for 24 h, thus greatly enlarging the clinic application range of the human mesenchymal stem cells. The components used in the solution accord with the clinic application, and can meet the requirement for clinic use of the human mesenchymal stem cells.

Pharmaceutical compositions and methods for using are provided for restoring β-cell mass and function in a mammal in need thereof. The pharmaceutical compositions have a biological response modifier and a β-cell growth factor in admixture with a pharmaceutically acceptable carrier, adjuvant or vehicle.

Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.

This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest. The rationally designed media may contain a baseline-adjusted concentration, A, of at least one amino acid that is calculated according to the formula A=[(M*X)+(N*P)+(Y*M*X)]*F, wherein X is the concentration of the amino acid that is used per unit of cell mass, P is the concentration of the amino acid that is used for incorporation into the polypeptide of interest per unit of polypeptide titer, M is the multiplier for the desired peak cell density of the cell culture, N is the multiplier for the desired concentration of the polypeptide of interest, Y is the cell maintenance factor; and F is the baseline factor. This rationally designed media may also be used to produce a starting cell culture medium comprising a concentration, B, of at least one amino acid according to the formula B=[A−(Z*V)] / (1−V), wherein Z is a concentration of the amino acid in the feeding cell culture medium, and V is a volume of the feeding culture medium as a proportion of the desired cell culture medium volume.

The invention discloses a cervical cell pathological slice automatic judging method with high and low resolution combination, which is characterized in that the method comprises the following steps: extracting a single cell mass region on a low-resolution cervical pathological slice image; extracting a single cell mass region from the low-resolution cervical pathological slice image; identifying suspicious abnormal cell clusters from low-resolution cell clusters; mapping suspected abnormal cell mass regions into high resolution slice images; semantically segmenting pathological cells from thehigh-resolution region of suspicious cell clusters and interpreting the type; according to the type, quantity and confidence of the segmented lesion cells, establishing the feature set of the slice, and then classifying the lesion type of the whole slice. The invention takes cell cluster as processing and recognition unit, utilizes classification neural network model to quickly identify suspiciousabnormal cell cluster on low-resolution digital slice, then divides out pathological cell on high-resolution suspicious area and judges its type, at the same time, improves recognition accuracy and recognition efficiency.

The invention discloses a preparation method of a dual mode (two mode signals-electrophysiological signal and electrochemical signal) ultramicro planar array sensor for quantitative assay of nerve cell quantum release. An ultramicro planar electrode (0.5-5 mum) is prepared through combination of a double layer wiring design, a high precision stepping photolithography technique and a partition isolation design. According to the method, orientated nanometer modification, biological compatibility modification and specific recognition enzyme modification are combined and performed on the surface of an electrode array so as to prepare the dual mode planar ultramicro electrode array sensor. The dual mode planar ultramicro electrode array is prepared by the method combining a micro electromechanical system technique, a nanometer modification technique and a biological modification technique. Through adoption of the method, the limitations of large electrode site size (10-50 mum) of a conventional planar microelectrode and single site detection of a rodlike carbon fiber electrode are broken. The ultramicro planar electrode array prepared by the method has small electrode site and multiple recording points, does not damage nerve cells, and can simultaneously detect neurotransmitter quantum release of a plurality of nerve cells and dual mode information of an electrophysiological action potential signal in real time in situ.

The present invention provides a culture vessel for forming an aggregated cell mass which has at least two wells, wherein the wells are made from a light-shielding member and an inner surface of the wells is subjected to treatment to impart cellular non-adhesiveness.

The invention discloses an adipose-derived stem cell separation culture method comprising the following steps of: separating and acquiring from adipose tissues; and culturing SVF (Stromal-vascular fraction) cells. The separation culture method is characterized by also comprising the following steps of: removing Lin+cells included in the SVF cells to obtain an Lin-cell mass by using a magnetic-activated cell sorting method; gathering CD271+Sca-1+cells from the obtained Lin-cell mass to obtain the adipose-derived stem cells by using a fluorescent-activated cell sorting method; and culturing theobtained adipose-derived stem cells by using a culture medium containing LIF (Leukemia Inhibitory Factor) and FGF2 (Fibroblast Growth Factor). The invention can efficiently and fast obtain the high-purity adipose-derived stem cells by combining FACS (Fluorescent-Activated Cell Sorting) with MACS (Magnetic-Activated Cell Sorting), wherein the high-purity adipose-derived stem cells have higher clone forming ability, self-renewal ability and multi-directional differentiation potentiality; P16 generation adipose-derived stem cells obtained by the separation culture method still have higher adipose forming ability and bone forming ability; in addition, the invention provides a new method for acquiring a large quantity of seed cells with dryness in vitro to apply to tissue regeneration.

The invention discloses a cell image processing method. The method comprises the steps of preprocessing an image, and separating a cell mass from a background by using an Ostu threshold method and a morphological operation; detecting out candidate cell nucleus centers by using a multi-scale LoG operator, extracting patches around the nucleus centers, screening out true positive cell nucleuses through a convolutional neural network, and segmenting true positive cells by using a level set to obtain boundaries of the cell nucleuses; for segmentation of overlapped cytoplasms, separating all cellsbased on a superpixel multi-cell tag segmentation method, allocating cell tags to all superpixel regions after superpixel image over-segmentation, taking the regions of the same tags as initial contours of the cells, and obtaining more accurate cell boundaries by using improved level set evolution. According to the method provided by the invention, the segmentation problem is converted into the classification problem; the problem due to weak cytoplasm edges is effectively solved; and the segmentation accuracy is improved. The method is high in running speed and has relatively good robustness.The invention furthermore discloses a cell image processing apparatus.

The embodiment of the invention provides a method and a device for updating a CoMP (Coordinated Multi-point Transmission) sending set. The method for updating the CoMP sending set comprises the following steps: sending a 1k rule to UE (User Equipment) by an RNC (Radio Network Controller) and receiving a measurement report sent by the UE when the 1k rule is met, and updating the CoMP sending set of the UE by the RNC according to the measurement report, wherein the 1k rule contains any following condition for the UE to send the measurement report: cell mass of a cell which belongs to an activating set and does not belong to the CoMP sending set is more than the cell mass of at least one cell in the CoMP sending set, or cell mass of a cell which belongs to an activating set and does not belong to the CoMP sending set is more than an average value or weighted average value of the cell mass of at least two cells in the CoMP sending set. The method provided by the embodiment of the invention can be used for updating the CoMP sending set so as to flexibly adjust CoMP cooperated cells, thereby being beneficial to further improvements of the whole property of cells and the performance of cell edge users.

The invention belongs to the biotechnological field and relates to a closed photo bioreactor for enhancing the light energy utilization rate of microalgae mass culture. At least two baffles vertical to the flowing direction of a culture solution are arranged on the inner wall surface of an illumination surface of the closed photo bioreactor, and a region between the baffles also covers a part of or all the region of an effective illumination surface between a culture solution inlet and a culture solution outlet of the photo bioreactor; the length of each baffle in a platy photo bioreactor is 30-100 percent of the width of a culture solution flow passage in the platy photo bioreactor; the length of each baffle in a round tube type photo bioreactor or an elliptical tube type photo bioreactor is 30-100 percent of the cross section arc length of an illumination surface of the culture solution flow passage. The closed photo bioreactor can efficiently enhance the light energy utilization rate of microalgae cell mass culture and improve the yield of microalgae cells, is suitable for culturing various microalgae in a large scale and produces relative products.

The invention discloses a culture method, application and a combined preparation of a hypoxia mesenchymal stem cell. By adopting an umbilical or placenta mesenchymal stem cell and combining the means of transfusing compound amino acid injection and the like, the vitality, the implantation survival rate and the treating effect of the mesenchymal stem cell in vivo can be improved; and in order to avoid the possibility of conglutination, conglomerating and cell mass embolism of the mesenchymal stem cell in blood vessel, the matching of saline containing heparin during treatment can be carried out by a mode of intravenous injection or intravenous drip. With more than three times of treatment, physical conditions of curee can be remarkably improved and objective indicators such as immunity, blood fat, blood sugar and the like can be remarkably improved.

The invention discloses a hybrid liriodendron somatic embryogenesis synchronization control method. In the method, 2,4-D concentration is reduced gradually by utilizing a growth hormone regulating mechanism; kinetin (KT) with the dose of 0.5 mg.L<-1> is added to induce uniform embryonic cell masses; the embryonic cell masses are subjected to fractional culture by a mechanically sieving method; and different subsequent synchronization control methods are adopted according to different cell mass sizes and somatic embryogenesis modes. After the synchronization control, the somatic growth synchronization ratio of hybrid liriodendron subjected to classified screening at the stage of globular embryo reaches about 90 percent; the synchronization ratio of fractional treatment at the stages of heart-shaped embryo and torpedo-shaped embryo is about 40 percent; after a proper period of refrigeration, the synchronization ratio is increased to some extent; and the conversion rate of a mature embryo, which develops from a material at the stage of globular embryo, to a regeneration plant can reach 70 to 80 percent.

The invention discloses aroma-enhancing puffed tobacco stem particles and a preparation method and application thereof. The preparation method of the aroma-enhancing puffed tobacco stem particles comprises the steps that a micropore structure is formed in each tobacco stem, and the tobacco stems are dried and smashed after being fixed and formed to form particles; the tobacco stem particles are inoculated with a compound aroma-generating microorganism, fermental cultivation and drying are carried out on the tobacco stem particles and the compound aroma-generating microorganism, and then the aroma-enhancing puffed tobacco stem particles can be obtained. The compound aroma-generating microorganism is formed by compounding bacillus subtilis, Trichoderma reesei, Aspergillus niger and Hansenula polymorpha according to the wet-cell mass ratio of 5:2:1:1. According to the aroma-enhancing puffed tobacco stem particles and the preparation method and application thereof, the tobacco stems serve as raw materials to be processed to obtain the puffed tobacco stem particles, then the compound aroma-generating microorganism is added, and serving as a filter material of a filter tip, the finally-obtained aroma-enhancing puffed tobacco stem particles have the advantages of being high in filter efficiency, strong in adsorption capacity, free of offensive odors and consistent with tobacco in aroma and have good application prospect.

A molecular marker for detecting a cancer stem cell in a cell mass which is a subject of interest, wherein the molecular marker can be detected in a cancer stem cell contained in the subject of interest but cannot be detected in a normal cell and a cancer cell that is different from a cancer stem cell; a method for determining the presence or absence of a cancer stem cell in a subject of interest by using the molecular marker as an measure; a kit for determining the presence or absence of a cancer stem cell, which comprises at least a reagent for detecting the molecular marker; a polypeptide encoded by the molecular marker; an antibody capable of recognizing an epitope of an expression product of a gene derived from the molecular marker; a nucleic acid capable of inhibiting the expression of the molecular marker; and a nucleic acid vaccine comprising a gene derived from the molecular marker.

The invention provides a capillary tube sampling system and method and a single-cell electrical characteristic detection system. The capillary tube sampling system comprises an injection pump, a capillary tube, a micro-fluidic chip and a negative-pressure module, wherein a compression channel, a cell recovery channel and a negative-pressure channel are sequentially formed in the micro-fluidic chip and constitute a micro-channel of the micro-fluidic chip; the front end of the compression channel is taken as a liquid inlet of the micro-channel, the rear end of the compression channel is connected to a liquid outlet of the micro-channel through the cell recovery channel and the negative-pressure channel, and the negative-pressure module is connected to the liquid outlet of the micro-channel; the rear end of the capillary tube is connected to the injection pump, inhalation and discharge of a cell suspension are controlled by the injection pump, and the cell suspension dropped in from one side of the liquid inlet of the micro-channel by the capillary tube sequentially enters the compression channel and the cell recovery channel under the action of the negative pressure provided by the negative-pressure module. According to the capillary tube sampling system and method and the single-cell electrical characteristic detection system, the loss rate of samples can be effectively reduced, the high recovery rate can be realized, and the single-cell electrical characteristic detection system can be used for electrical characteristic detection of cell samples with extremely small quantity of cells.

A method for inducing the differentiation of stem cells into tissue cells; a bioartificial organ, which contains the tissue cells; and a material for medical purposes, which contains the bioartificial organ are disclosed. Stem cells capable of proliferation, self-replication and differentiation are used and cultured by a hanging-drop method to three-dimensionally construct a cell mass of embryoid bodies, and the cell mass is cultured in the presence of HGF, GDNF, b-FGF, BMP7 and EGF. In this manner, the stem cells can be differentiated into tissue cells. A bioartificial organ can be produced using cells that have been differentiated into the tissue cells. Further, a material for medical purposes can be provided.

The invention discloses a preparation method of silver / silver alloy microwire. The preparation method comprises the following steps: adding metallic elements with the concentration of 0-4wt% such as metal indium (In), copper (Cu), stannum (Sn) and zinc (Zn) to metal Ag with the high purity of 99.99% so as to form alloy, carrying out continuous casting, coarse drawing, intermediate drawing, mini-fine drawing and micro-drawing on a sliver / sliver alloy material and annealing, thus obtaining the microwire with the minimum diameter of 0.010-0.020mm. The silver / silver alloy microwire is superfine in wire diameter, has good mechanical property and electrical property and meets the requirement on the tensile strength of the microwire in a layout process and the requirement on the processing property of a material required in photovoltaic cell mass production. The silver / silver alloy microwire does not warp and fall off in sintering and annealing processes, has good interfacial adhesion with silver paste after being sintered, and can be applied to front electrode materials of Si solar photovoltaic cells.

The invention discloses a somatic embryogenesis method for cunninghamia lanceolata, which comprises the steps of initial induction of an embryogenic suspensor cell mass, maintenance and multiplication of an ESM (embryogenic suspensor mass), and development and maturity of a somatic embryo, wherein the development and maturity of the somatic embryo can adopt an indirect somatic embryogenesis way or a direct somatic embryogenesis way. With the adoption of the somatic embryogenesis method for the cunninghamia lanceolata, browning in an establishment process of the conventional liquid suspension system can be effectively improved, cell division under a low density condition is started, and the stability of suspensor cells is maintained, so that a stable multiplication liquid suspension cell line is established. The somatic embryogenesis frequency of the cunninghamia lanceolata can be increased by about 20 times at most through the direct and indirect somatic embryogenesis ways, the problem of low somatic embryogenesis frequency caused by an incomplete somatic embryogenesis system of the cunninghamia lanceolata can be well solved, the cost is saved, conditions are prepared for factory production of the cunninghamia lanceolata, and a pace of industrial production of the cunninghamia lanceolata is accelerated.

Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.

A culture system has a culture bag for suspending and culturing pluripotent stem cells in a culture medium; a waste liquid container for storing used culture medium that is connected to the culture bag; a fresh medium container for storing a fresh culture medium that is connected to the culture bag; three-way stopcocks for switching flow channels from the culture bag to the waste liquid container or the fresh culture medium container, etc.; a trap portion for trapping the pluripotent stem cells in the culture medium in the flow toward the waste liquid container side; and a filter portion for subculturing that is formed in fourth and fifth flow channels in parallel to a first flow channel. The first flow channel connects the trap part to the culture bag, and has a mesh by which a cell mass of the pluripotent stem cells can be divided. A pump is used to pump the liquid in the individual flow channels.

The invention discloses an ultralow temperature preservation method and an activity identification method for a cardiocrinum giganteum var. yunnanense protoplast. The ultralow temperature preservation method comprises the steps of (1) inducing a callus, (2) separating the cardiocrinum giganteum var. yunnanense protoplast, (3) purifying the cardiocrinum giganteum var. yunnanense protoplast, and (4) freezing, unfreezing, and obtaining the preserved protoplast. The activity identification method comprises the steps of identifying activity, nursing and culturing a protoplast solid, observing division of the protoplast under a microscope, culturing for 2-3 days to see a regenerated cell wall of the protoplast, culturing for 4-6 days to see a firstly-divided cell, and culturing for 30-45 days to see a regenerated cell mass of the protoplast. With the adoption of the ultralow temperature preservation method and the activity identification method, a novel scientific approach is provided for the long-term preservation of cardiocrinum giganteum var. yunnanense which is valuable, rare and endangered and integrates viewing, edible and medicinal values.

The invention belongs to the technical field of cell immunity, and particularly relates to a preparation method of a human D-CIK (dendritic cell activated and cytokine induced killer) cell with high toxicity and high value-adding capacity. The preparation method comprises the following steps of 1) collecting peripheral venous blood so as to obtain a single karyocyte; 2) further separating the single karyocyte so as to obtain a mononuclear cell and a suspension cell; 3) induced differentiating the mononuclear cell to a mature DC (dendritic cell); 4) induced culturing CD3+CD8+CIK cells; 5) induced culturing the D-CIK cells so as to obtain a novel heterogeneity cell mass, i.e. D-CIK cells. Compared with the CIK cell, the value-adding activity and the cell toxic activity of the prepared D-CIK cell are stronger, and the prepared D-CIK cell also has the advantages of simple preparation process, low cost, and the like, and mass production is easy to realize. The prepared D-CIK cell is mainly used for treating cancer patients or preventing the cancer high-risk group.