The invention discloses a method for efficiently regenerating
plant through Hedychium coccineum Buch.-Ham
somatic embryogenesis. The method comprises the following steps of: inducing calluses by using immature filaments and anthers of Hedychium coccineum Buch.-Ham as explants, conducting optimization, screening and subculture to obtain high-quality embryonic calluses, conducting
suspension culture to the embryonic calluses and establishing an embryonic
cell suspension culture system of Hedychium coccineum Buch.-Ham. A great number of somatic embryos can be obtained by using embryonic
cell suspension cultures for inducing somatic embryos, an optimum effect can be obtained by using SH inorganic salt and 150mug.L<-1> TDZ, and the somatic
embryo generation rate can reach 4429 / ml SCV (settled
cell volume); the obtained somatic embryos can germinate seedlings with buds and roots on somatic
embryo germination culture medium; and when SH culture medium is used as basic culture medium and the concentration of 6-BA is 0.5-1.0mg.L<-1>, the
germination rate of the somatic embryos can reach 100 percent. Due to the method, a foundation is laid for the in-vitro rapid propagation and the biotechnological breeding of Hedychium coccineum Buch.-Ham, the large-scale popularization and application are facilitated and the application prospect is better.