46 results about "Epicotyl" patented technology

The invention discloses a method for promoting efficient germination of seeds of a valuable and rare endangered plant yunnanopilia longistaminea, wherein the method comprises the steps: collecting ripe yunnanopilia longistaminea seeds; removing seed coats, and breaking inhibition of the seed coats; carrying out cleaning and disinfection treatment on the seeds; carrying out root promoting culture, and soaking the seeds with a 50 mg / L GA3 solution for 24 h; soaking the seeds with a 100 mg / L GA3 solution for 24 h, placing the seeds at the temperature of 5 DEG C, and refrigerating for 7-14 days to change endogenous hormone change of the yunnanopilia longistaminea in seed germination and seedling building processes, so as to break epicotyl dormancy; placing the seeds in the environment of the temperature of 28 DEG C, and culturing; culturing for 3-4 weeks to obtain seedlings having euphylla grown; and providing the seedlings for wild transplanting after growing for 8-10 weeks. The system method directly adopting the yunnanopilia longistaminea seeds for fast and efficient cultivation of the seedlings effectively relieves the epicotyl dormancy of the yunnanopilia longistaminea, ensures that the germination rate of the yunnanopilia longistaminea seeds is more than or equal to 70%, effectively shortens the seedling culture time by 60 days, and is simple and convenient to operate, high in survival rate, and strong in practicability. An effective technical means is provided for species protection biology, restoration ecology, resource exploitation and utilization and the like.

The invention discloses a direct polygerm-generation regeneration plant culture method by the isolated culture of Xinjiang snow locus epicotyl. The invention also discloses a decapitated epicotyl exopThe invention discloses a direct polygerm-generation regeneration plant culture method by the isolated culture of Xinjiang snow locus epicotyl. The invention also discloses a decapitated epicotyl exophyte. The invention utilizes an epicotyl exophyte and the decapitated epicotyl exophyte to establish a gene-independent, simple and rapid Xinjiang snow locus isolated culture regeneration plant culturhyte. The invention utilizes an epicotyl exophyte and the decapitated epicotyl exophyte to establish a gene-independent, simple and rapid Xinjiang snow locus isolated culture regeneration plant culture technology which directly induces polygerm generation, elongation and rootage; by the formulation of a culture medium and the optimizing screen of carbon sources, the browning degree of the exophytee technology which directly induces polygerm generation, elongation and rootage; by the formulation of a culture medium and the optimizing screen of carbon sources, the browning degree of the exophyteis lowered to the lightest degree, an exophyte can have the maximum generation bud number more than or equal to 3 and the regeneration bud rootage rate higher than 90 percent, and a regeneration plan is lowered to the lightest degree, an exophyte can have the maximum generation bud number more than or equal to 3 and the regeneration bud rootage rate higher than 90 percent, and a regeneration plant with a full root, a full stalk and full leaves can be obtained by only 3 to 4 months. The invention has low malformed seedling rate and lays a foundation for the hereditary improvement of Xinjiang st with a full root, a full stalk and full leaves can be obtained by only 3 to 4 months. The invention has low malformed seedling rate and lays a foundation for the hereditary improvement of Xinjiang snow locus breeds applied in production during gene engineering.now locus breeds applied in production during gene engineering.

The invention discloses a regeneration and cultivation method of high frequency somatic embryos for overcoming shamrock variety genotypic disorder, which comprises the following steps: 1. material selection: selecting three explants, i.e. an epicotyl, a hypocotyls and a regeneration plant leaf of an aseptic seedling after a mature seed of the shamrock is flushed, sterilized, disinfected and dried; 2. inoculating the selected explants onto an embryogenic callus inducing culture medium for cultivation; 3. inoculating the explants cultivated through the embryogenic callus inducement onto an embryo successive formation culture medium for cultivation; 4. inoculating the explants cultivated through the embryo successive formation cultivation onto an embryo maturation germination seedling culture medium for cultivation to form seedlings; 5. carrying out rooting cultivation on strong seedlings; and 6. transplanting a regeneration plant for survival. The invention can effectively overcome the problems of different varieties and genotypic disorder in the regeneration and cultivation of the high frequency somatic embryos of the shamrock, greatly improve the cell embryogenesis frequency and the regeneration plant frequency, and improve the breeding efficiency.

The invention discloses an epicotyl explant (epicotyl explant) of a cotton in vitro culture and direct multi-bud generation regenerated plant and a cultivation method for obtaining a complete regenerated plant by using the explant. The invention also discloses a decapitated epicotyl explant. The present invention utilizes epicotyl explants and fresh epicotyl explants to establish a technique for directly inducing multi-bud generation, elongation and rooting that does not depend on genotype, simple and fast cotton in vitro culture regeneration plant technology, Through the optimal screening of medium formula and carbon source, the degree of browning of explants is reduced to the lightest, the maximum number of buds in one explant is ≥ 3, and the rooting rate of regenerated shoots is above 90%, obtaining a plant with complete roots, stems and leaves. It only takes 3 to 4 months to regenerate plants, and the rate of deformed seedlings is low, which lays the foundation for the genetic improvement of cotton varieties used in production.

The present invention provides a method for constructing a highland barley-endophytic fungi symbiosis, comprising the following steps: (1) disinfection of highland barley seeds; (2) cultivation of sterile highland barley seedlings; (4) inoculation: on the sterile highland barley seedlings Artificial inoculation of the hypocotyl: vertically cut a small opening at the epicotyl of the sterile highland barley seedling, stuff the cultured mycelium into the small opening, and place it at a constant temperature (25±2)°C for dark cultivation for 7-10 days. Then at a constant temperature (25±2) °C, the light intensity is 2000~3000lx, and the light time is 16h·d ‑1 Cultivated under the conditions for 7-10 days to obtain highland barley seedlings; (5) transplantation: after the highland barley seedlings were washed root medium, they were transferred into the substrate and cultivated until the seeds were harvested; (6) endophytic fungi were detected: transplanted for a period of time The inoculation of endophytic fungi was then detected, the seedlings that were not successfully inoculated were eliminated, and the seeds were collected after the remaining seedlings were mature. The present invention constructs a highland barley-endophytic fungus symbiosis by inoculating grass endophytic fungi on the epicotyl of highland barley, and creates a new highland barley germplasm resource.

The invention discloses a method for cultivating a grafted tomato seedling with two sprouts. The method mainly includes the steps that a stock species which is resistant to stress and high in water absorption capacity and fertilizer absorption capacity and a scion species which is high in yield and good in quality are selected, the stock and the scion are cultivated to have 2-4 true leaves separately, tube grafting is conducted, after the grafted seedling completely survives, a cutter is used for making a horizontal slash on the embryonal axis portion of the scion of the grafted seedling, the true leaves and an apical growing point are removed, one two cotyledons are maintained, the grafted seedling continues to be cultivated to sprout in the axil, and two side branches are generated. Compared with a traditional grafted tomato seedling, the two side branches which are uniform in growth vigor can be generated, after planting, the two side branches become two fruit bearing main vines, and the good-quality and high-yield cultivation of tomatoes is easily achieved.