737 results about "Citrus volkameriana" patented technology

The invention provides bacillus methylotrophicus with preservation number-CCTCC NO. M2013500. The invention also provides a microorganism bacterium agent. The microorganism bacterium agent contains a culture medium and thallus, wherein the thallus comprise the bacillus methylotrophicus with preservation number-CCTCC NO. M2013500, bacillus subtilis, bacillus lincheniformis and bacillus amyloliquefaciens. The invention also provides an application of the bacillus methylotrophicus and the above microorganism bacterium agents to improvement of saline lands, improvement of plant drought tolerance, prevention of bacterial wilt of pepper and prevention of Citrus anthracnose.

Disclosed is a high-efficiency lycoris and citrus interplanting method. The high-efficiency lycoris and citrus interplanting method includes interplanting lycoris under fruit trees such as citrus, covering the lycoris by rotten animal manure straws, managing by processes of fertilizing, weeding, insecticiding and the like, and harvesting after five-year planting. By the high-efficiency lycoris and citrus interplanting method, two kinds of economic plants can be produced on the same field, multiple cropping index is increased, labor cost is saved, production cost is reduced, yield is increased, and economic benefits are doubly increased.

The invention provides a preparation for orange bud picking and tip control. The preparation consists of the following components in part by weight: 1 to 20 parts of lactofen, 20 to 40 parts of solvent 1 xylene, 10 to 20 parts of solvent 2 propyl alcohol and / or benzene, and 30 to 60 parts of synergist one or any combination of more of triton X-100, lignosulfonate, agricultural organic silicon surfactant, calcium dodecylbenzene sulfonate, alkylphenol ethoxylate and octachlorodipropyl ether. The invention also provides a preparation method and a using method of the preparation. The preparation has good effect of killing orange buds and delicate tips; the death rate of the buds is 100 percent, the death rate of the delicate tips and half-mature new tips is over 95 percent, and the yield of the orange fruits is increased by 18.2 to 52.3 percent; moreover, the preparation does not have any side effect on other tissues or organs such as the orange fruits, mature branches and leaves, and thelike, and is convenient to use, and the work efficiency is improved by more than 15 times.

The invention relates to the technical field of plant separation and extraction, and in particularly discloses a method of combined extraction of essential oil, pectin, hesperidin, synephrine and limonin from citrus. The method comprises the following steps: inactivating the citrus, buffing and performing enzymolysis to obtain essential oil, a water phase and skin residues; separating the water phase through strong acidic cation-exchange resin to collect effusive liquid, and eluting to obtain synephrine; separating the effusive liquid using ultra-filtration method to obtain pectin and liquid passing through the ultra-filtration film; refluxing and extracting the liquid passing through the ultra-filtration film using ethanol to obtain supernatant and filter residue; crystallizing, and freeze-drying the filter residues to obtain aurantiamarin; separating the supernatant using macroporous resin, and eluting using ethanol to obtain limonin. The method is used for processing fresh citrus to co-produce the citrus pericarp essential oil, pectin, hesperidin, synephrine and limonin; the utilization rate of the resource is improved, the environment pollution is reduced, and the economic and social benefits are notable.

The invention belongs to the plant protection subject of the agricultural category, and particularly relates to citrus yellow shoot candidatus liberibacter asiaticus detection test paper. The detection test paper is prepared through the following steps that pathogenic bacteria antigen is extracted and purifed from catharanthus roseus host with citrus yellow shoot candidatus liberibacter asiaticus antigen and used for injecting immunized rabbits to obtain yellow shoot candidatus liberibacter asiaticus specific antiserum, the yellow shoot candidatus liberibacter asiaticus specific antiserum is purified to obtain the citrus yellow shoot candidatus liberibacter asiaticus antigen, immune colloidal gold is prepared, a combined pad which is soaked with the immune colloidal gold and a chromatography membrane which is coated with a detection line T-citrus yellow shoot candidatus liberibacter asiaticus antigen and a quality control line C-goat anti-rabbit antibody are further prepared, and finally a detection test paper product is assembled. Against for the requirement of the detection of a great amount of citrus yellow shoot samples, the citrus yellow shoot candidatus liberibacter asiaticus detection test paper which is convenient, rapid and high in specificity is provided. The test paper has following advantages that 1, convenience in use and simpleness in operation are realized; 2, a detection result can be obtained within a short period of time; 3, the stability is good; and 4, the cost is low, and the test paper is particularly suitable for the discrimination of citrus yellow shoot plants of a fruit garden.

The invention relates to the technical field of agricultural planting, in particular to a composite microbial agent for citrus tristeza virus, a preparation method thereof and a special biological agent for the citrus tristeza virus prepared by using the same. According to the invention, bacillus thuringiensis, beauveria bassiana and trichoderma harzianum are used to control the pathogen of citrustristeza virus pathogens on the ground and kill a spreading source wood louses and eggs thereof so as to effectively control the occurrence of the citrus tristeza virus, and four kinds of microorganism bacteria underground can effectively control the invasion of pathogenic microorganisms, effectively control the spread of pathogenic bacteria by nematodes, and simultaneously regulate the soil structure, decompose organic matters and improve the soil fertility. By promoting the growth of citrus roots with citrus specific AM fungi, citrus can grow new roots in clusters, the number of the new roots is enlarged, the tree vigor is enhanced, the immunity and disease resistance of trees are improved, a nitrogen fixation system is formed, and the pathogen control is combined with the improvement of the ecological environment for the growth of the citrus to achieve the effect of preventing and treating the citrus tristeza virus.

The invention discloses honey osmanthus tea with effects of nourishing lung and lubricating intestines. The honey osmanthus tea is prepared from the following raw materials in parts by weight: 15-20 parts of osmanthus, 13-16 parts of green tea, 100-120 parts of rice wine, 500-530 parts of distilled water, 60-70 parts of loquat, 50-60 parts of citrus, 30-40 parts of snow pear, 200-240 parts of honey, 50-60 parts of almond, 25-30 parts of needle mushroom, 15-20 parts of kelp, 5-6 parts of polygonum multiflorum and the like. The honey osmanthus tea with effects of nourishing lung and lubricating intestines, which is disclosed by the invention, is added with the osmanthus and kelp and has the effects of invigorating stomach and promoting the secretion of saliva or body fluid, expelling toxins and lubricating intestines and the like, and due to addition of extracting solutions of the polygonum multiflorum, cordyceps sinensis, burdock, violet and mint, the honey osmanthus tea is endowed with excellent effects of nourishing lung and lubricating intestines and can be beneficial to body health after being drunk for a long time.

The invention belongs to the field of plant transgenic technique, particularly relates to a cultivating method of a citrus transgenic plant and discloses a method for cultivating the citrus transgenic plant by utilizing agrobacterium mediation and taking a citrus test-tube seedling blade as an explant. The method comprises the following steps of genetic transformation, screening and regeneration,PCR (Polymerase Chain Reaction) detection and GFP (Green Fluorescent Protein) fluorescence observation. The method is characterized in that a GFP reporter gene is guided into the receptor citrus blade through agrobacterium mediation, different factors capable of influencing the blade transformation are screened, kanamycin is utilized as a selector to screen a transformed receptor cell, and the transgenic citrus plant is screened and obtained through the PCR detection, the GFP fluorescence observation and other auxiliary methods. The method provides a new technical platform for innovative germplasm resources of citrus genetic transformation.