89results about How to "The separation and purification method is simple" patented technology

The invention relates to a magnetic composite nanoparticle with selective adsorption to antibodies. The structural formula of the magnetic composite nanoparticle is shown as the specification. The method includes: taking an Fe3O4 nanoparticle with superparamagnetism as the core, using tetraethyl orthosilicate as the silicon source, and employing SiO2 or a polymer to coat the Fe3O4 magnetic nanoparticle, thus obtaining a core-shell structure Fe3O4SiO2 or Fe3O4 polymer magnetic composite particle; and bonding a silane coupling agent mercaptoethyl trimethoxy silane to the silica gel surface of the magnetic composite particle, and then carrying out mercapto-ene click reaction on product and double bond-containing vinylpyridine (MEP), thus obtaining the Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle adsorbent. The Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle is a novel bio-functional magnetic composite nano-material with selective adsorption to antibodies. The material has very good superparamagnetism, and can rapidly realize solid-liquid separation under the effect of an external magnetic field, also shows specificity to antibody adsorption, and can be used for rapid separation and preparation of antibodies.

The invention belongs to the field of the extraction of natural medicines and particularly relates to a limonin analogue and a separation and purification method thereof. The invention aims to solve the technical problem of providing a fire-new separation and purification method of a limonin analogue. According to the separation and purification method disclosed by the invention, the medicinal materials of rue family citrus plants, such as tangerine seeds, pummelo seeds, immature bitter oranges, tangerine peels and the like, are used as raw materials, 40 to 50 percent of ethanol is used as a solvent for extraction, an extract is separated and purified by adopting HPD-722 type macroporous resin, the content of the limonin analogue in obtained solid matters is greater than 80 percent, the content of limonin is greater than 20 percent, and the content of nomilin is greater than 40 percent. The method has high extraction efficiency, little reagent consumption and little pollution and is simple to operate.

A method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc. The invention relates to a method for separating purified seabuckthorn flvonoid from large berry seabuckthorn marc. The invention solves the problems in the existing separation and purification method of seabuckthorn flavonoid, such as tedious method, complicated techniques, high cost of separation and purification, easy damage to the activity of flavonoid and low purity of flavonoid. The method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc disclosed by the invention comprises the following steps: first, degreasing the raw materials; second, ultrasonic wave extracting and centrifugating; third, adding metal salt to extracting solution to lead complex reaction between flavonoid and metal, agitating, tempering the pH of solution and collecting sediment; and fourth, adding decomplexing additive to resolve sediment, concentrating in a decompressing way to dryness, using ethanol to dissolve and filter, concentrating filtrate, and obtaining the separated and purified seabuckthorn flavonoid powder after drying. The method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc disclosed by the invention can ensure the full improvement of the purity of the purified seabuckthorn flavonoid, and maintains the original biological activity of the natural flavonoid, the seabuckthorn flavonoi is safe and nontoxic, and the method disclosed by the invention has the advantages of low production cost and simple process, and is suitable for the large-scale production of the seabuckthorn flavonoid.

The invention relates to the technical field of detection materials, in particular to a near-infrared bio-thiol fluorescent probe as well as a preparation method and an application thereof. The structural formula of the near-infrared bio-thiol fluorescent probe is shown in the description. The preparation method of the near-infrared bio-thiol fluorescent probe comprises the following step: the target compound is prepared from a compound A and 2,4-dinitrobenzenesulfonyl chloride by a reaction, wherein the structural formula of the compound A is shown in the description. The compound almost hasno fluorescence but emits intense fluorescence after a reaction with bio-thiol, the fluorescence emission wavelength is larger than 650 nm and is in the near-infrared region, and the fluorescence canbe distinguished from autofluorescence of body tissue and can effectively penetrate the tissue to eliminate biological background interference. The compound can be used for detecting bio-thiol, is simple to operate, has fast and sensitive double-response and has broad application prospects in the fields of biomolecular detection and environment.

The invention relates to a preparation method of esamin phenol. Sesamolin reacts in a benzene series solvent under the action of a catalyzer at the temperature of 70-110 DEG C for 1-6h, and sesamin phenol is obtained after a reaction solution is treated, wherein the catalyzer is an H-type strongly acidic styrene which is cation exchange resin. Compared with the traditional methods, the preparationmethod has the advantages of available raw material, stable conversion process, high sesamin phenol yield, simple separation and purification, green process and the like.

The invention discloses a method for improving foaming property and foam stability of protein. According to the method, high-temperature heating is performed on whey protein concentrate to obtain a nano-fiber polymer, wherein the foaming property and the foam stability are obviously improved. The prepared nano-fiber polymer as an inducer and the whey protein concentrate are mixed and then subjected to heat treatment, so that the foaming property and the foam stability of the whey protein concentrate can be further improved. The whey protein concentrate fiber polymer is hydrolyzed with enzymes,the obtained hydrolysate as the inducer and the whey protein concentrate are mixed and then subjected to high-temperature heating treatment, and results show that the foaming capacity of the treatedwhey protein is improved by 47.73-59.09%, and the foam stability is improved by 84.98-101.1%. The method is low in production cost, the raw materials are broad in source, complex equipment is not needed, the technology is simple, and the method is easy to popularize and apply.

The invention relates to a separation and refining method of arsenic trioxide. According to the method, a arsenic trioxide crude product is used as raw material and undergoes the following steps: (1) esterification reaction; (2) solid-liquid separation; (3) adsorption and impurity removal; (4) solid-liquid separation; (5) rectification and impurity removal; (6) hydrolysis-crystallization; (7) solid-liquid separation; (8) washing; (9) dehydration; and (10) drying. Thus, high-purity arsenic trioxide is obtained. The separation and refining method has the following advantages: process flow is short; the separation and refining method is simple; product purity is high; quality is stable; the process is safe and reliable; energy consumption is low; the amount of ''three wastes (waste gas, waste water and industrial residue)'' is small; and the separation and refining method is a clean production method which is adopted for the preparation of high-purity arsenic trioxide and accords with development requirements of the green chemical industry.

The invention provides a preparation method for a polyclonal antibody against chicken caspase-1. According to the preparation method, caspase-1 recombinant protein is used as an antigen for immunization of an animal so as to obtain the polyclonal antibody against chicken caspase-1. According to the invention, chicken caspase-1 is used as a research object; the polyclonal antibody against chicken caspase-1 prepared through gene amplification and construction, expression, purification and identification of a recombinant vector can specifically recognize target protein, so a foundation is laid for deep research on the toxicological significance of the family of chicken caspase-1 genes and the role played by the caspase-1 genes in virus-mediated innate immune response, and a novel target is provided for research on novel vaccines and development of medicines. Meanwhile, the prokaryotic expression vector constructed in the invention has high efficiency in expression of recombinant protein, and a separating and purifying method thereof is simple, low in cost and short in period.

The invention discloses a sulfadimidine affinity membrane; a preparation method of the sulfadimidine affinity membrane comprises the following steps: (1) taking a cellulose acetate membrane, completely hydrolyzing under an alkaling condition, then adding epoxy chloropropane to perform epoxy activation, and then using deionized water to wash to be neutral after reaction under a suction filter condition, so as to obtain the epoxy activated membrane; (2) dissolving the sulfadimidine into an alkaline buffer solution with pH of 10-12, then dispersing the epoxy activated membrane obtained in the step (1) in the above-mentioned solution, reacting at the temperature of 50-65 DEG C for 24h-36h, then using deionized water to wash under the suction filter condition to make UV absorption of a percolate at 280nm close to zero, and then drying the solution to get the sulfadimidine affinity membrane. The sulfadimidine affinity membrane has good adsorption performance for an antibody, and is particularly suitable for separating and purifying immune globulin IgG.

A preparation method for N-methylisatoic anhydride rapidly-labeled polysaccharide belongs to the technical field of fluorescent labeling. The preparation method employs a fluorescence tracer N-methylisatoic anhydride for labeling polysaccharide, and the method comprises: performing incubating on a N-methylisatoic anhydride stock solution and a polysaccharide solution under the condition of 35 DEG C for 15-20 min, and performing separation purification on the fluorescence-labeled polysaccharide through an organic solvent precipitation process. The reaction is mild in reaction conditions and short in reaction time, and the fluorescence-labeled polysaccharide separation purification process is simple. Polysaccharide labeled by fluorescence can be detected by using a routine fluorescence detector, and research on polysaccharide functions and mechanism is facilitated.

The invention provides a separation and purification method for a pertussis antigen. The separation and purification method comprises the steps of with diatomite and hydroxylapatite as column chromatography materials, after concentrating, regulating and conducting a bordetella pertussis fermentation solution, enriching the pertussis antigen by using the diatomite, and then, purifying by using the hydroxylapatite to obtain pertussis toxin (PT) and filamentous hemagglutinin (FHA). Proven through SDS-PAGE electrophoresis detection, the purities of both a PT protein and an FHA protein are higher than 95%. The method provided by the invention is simple in operation and capable of obtaining a high-purity antigen, not only greatly reducing the production cost, but also remarkably improving the quality of a product and increasing the yield of the product so as to be the simple and rapid separation and purification method for the pertussis antigen; in addition, the economic benefit is high, and the market application prospect is wide.