The invention discloses a color-changing indicating error-proofing UV electronic adhesive. The color-changing indicating error-proofing UV electronic adhesive comprises following raw materials, by weight, 20 to 80 parts of a UV resin, 0.5 to 3 parts of an initiator, and 0.5 to 5 parts of a color-changing agent; the UV resin is selected from acrylic acid modified polyurethane, epoxy modified acrylate, UV silicone rubber resin, and epoxy resin of cyclic ester group; the initiator is selected from a free radical photoinitiator and a free radical thermal initiator; the color-changing agent is selected from a photochromic polymer with a thiocarbazone coordination compound structure, a photochromic high-molecular compound with an azobenzene structure, a photochromic high-molecular compound with a spirobenzopyran structure, and the like. The invention also discloses a preparation method of the color-changing indicating error-proofing UV electronic adhesive. Color of the color-changing indicating error-proofing UV electronic adhesive before solidification is stable; and difference of the color of the color-changing indicating error-proofing UV electronic adhesive before and after solidification is obvious, so that in production lines, it is possible to quickly identify that whether solidification of the color-changing indicating error-proofing UV electronic adhesive is realized, whether adhesive dispense positions are appropriate, and whether adhesive thixotropic response is induced. In addition, it is possible for direct determination of adhesive stability by associating color with adhesive solidification degree.