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1700results about "Monoazo dyes" patented technology

Non-fluorescent quencher compounds and biomolecular assays

Bis-diazo,triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl / diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides, and polypeptides. The quencher moieties are non-fluorescent and accept energy from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labelled biomolecules for various molecular biology assays based on fluorescence detection.
Owner:APPL BIOSYSTEMS INC

Process for synthesizing azo dispersion dyes monomer compound

The invention provides the synthesis process of an azo-disperse dyes monomeric compound, comprising the following steps: (1) diazotization of compound (II) and nitrosyl sulfuric acid is completed with the temperature being controlled between 1 DEG C and 25 DEG C; (2) water, emulsifying agent and compound (III) are added into a coupling reactor and stirring and pulping are completed at the temperature ranging from 10 DEG C to 25 DEG C; (3) diazonium salt is evenly added into the coupling reactor from below the slurry and is stirred till the end of the reaction, and then extraction filtration and washing are adopted till the slurry shows neutrality; finally, the azo-disperse dyes monomeric compound can be obtained after drying. Through improving preparation process, a great amount of ice or frozen salt water no longer needs to be added for cooling down during coupling reaction to meet the requirements; the temperature of coupling reaction is gradually raised to 20 DEG C to 45 DEG C by use of heat liberation of coupling reaction and diazonium salt dilution instead; thereby, under the condition of identical quality effect, cost is greatly reduced and at the same time, energy consumption during preparing cooling agent such as ice and frozen salt water is also substantially reduced.
Owner:SHANGYU JINGUAN CHEM IND +2

Compounds and methods for labeling oligonucleotides

The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below.The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.
Owner:INTEGRATED DNA TECHNOLOGIES
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