35 results about "Digoxigenin" patented technology

The preparation of vitamin D compounds of formula (I) with a label attached to a spacer group in the 3 position is disclosed. In the above formula (I), O represents the oxygen atom of an ether group; Y represents hydrogen or hydroxy; A represents a label such as biotin, digoxigenin, or another vitamin D group; R represents a substitured hydrocarbon side-group of vitamin D or a vitamin D metabolite. Also disclosed is a method of measuring 25-hydroxy vitamin D metabolite and a 1α,25-dihydroxy vitamin D metabolite in a sample.

A PCR-ELISA detection method for the vibrio parahaemolyticus in aquatic products is achieved by four steps: the extraction of sample DNA, the PCR reaction, the ELISA reaction and the result detection. By providing two groups of specific digoxigenin-labeled primers of vibrio parahaemolyticus tlh, tdh, a biotin-labeled probe for distinguishing two genes, and plasmid used in a quantitative specific sequence containing the tlh gene, the invention can further detect the vibrio parahaemolyticus in the aquatic products. The invention can be carried out in a 96-hole ELISA plate; and the provided method has low cost, good specificity and high sensitivity, and is suitable for conducting large-scale, high-throughput detection on the vibrio parahaemolyticus.

The invention discloses a compound polymerase chain-ELSA reaction prawn virus detecting method. Its steps: 1. design and synthesis of catch probe and amplifying primer; 2. extraction of tissue total nucleic acid; 3. labeling digoxigenin with compound polymerase chain reaction; 4. solid-phase cross; 5. enzyme linked coloration. It is adapted to diagnosis of white spot syndrome virus, taola syndrome virus and hepatopancreatic minute virus and monitoring of the health state of the prawn.

The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below.The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.

The invention discloses a kit for determining the sex of a sheep early embryo. The kit disclosed by the invention is provided with two pairs of specific primers, two probes and colloidal gold test strips, wherein the two pairs of specific primers are respectively SRY gene primers and interior label Callipyge gene primers, and the two probes are respectively an SRY probe and a Callipyge probe; each colloidal gold test strip is composed of a conjugate pad and a reaction pad; the conjugate pad is formed by coating colloidal gold on a BIOTIN antibody; the reaction pad is connected with the tail end of the colloidal gold conjugate pad, composed of a nitrocellulose membrane and respectively coated with a detection area and a quality control area; and the detection area is coated with an FITC (fluoresceinisothiocyanate) antibody, and the quality control area is positioned downstream from the detection area and coated with a DIG (digoxigenin) antibody.

Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

The invention relates to a specific-nucleic-acid-probe dot-blot-hybridization detection kit for tilapia streptococcus agalactiae and belongs to aquatic product biologicals. According to the kit disclosed by the invention, digoxigenin-labeled streptococcus agalactiae probes are synthesized from specific primers through PCR, and through dot molecular hybridization, the probes can be used for detecting whether streptococcus agalactiae specific nucleic acid exists in a pathological specimen or a supernatant of boiled strains or not and are used for judging whether streptococcus agalactiae infection exists or not. According to the kit disclosed by the invention, a pathological specimen tissue DNA extract or a supernatant of water-boiled strains to be detected is subjected to dot molecular hybridization, and detection and result reporting can be completed within 16-24 hours, so that the specificity is strong, and the accuracy is high.

The invention provides bioconjugates of heterocylic compounds such as S-adenosylmethionine and S-adenosylhomocysteine with biotin or digoxigenin. The bioconjugates also include carbon and nitrogen linker moieties of varying length that are used to attach such compounds to biotin or digoxigenin. The conjugates are useful in immunoassays. The invention provides a method for detecting SAM and SAH, comprising the steps of: (a) preparing the following components: (i) bio-conjugates of SAM, SAM analogs or SAH; (ii) an europium, a terbium cryptate or other fluorophore as a donor that has a specific ligand for the tracer in the bio-conjugates of (i); (iii) an acceptor fluorescent dye that has the excitation spectra overlap those of donor's emissions and has an antibody specific for SAM or SAH labeled; (b) addition of the biological fluid containing said SAM or SAH; and (c) spectroscopic measurement of the fluorescence of the donor and the fluorescence of from the acceptor.

The invention provides application of molecule miR-150 in screening preparation of a colorectal cancer detecting medicament. The molecule miR-150 is screened out by using a muParaflo microfluidic chip by the inventor of the invention; a paraffin section preparation of a clinical colorectal cancer patient is detected by an established digoxigenin labeled probe of miR-150 and an established detection method of qRT-PCR (Quantitative Real-Time-Polymerase Chain Reaction), respectively; results show that miR-150 low expression level in the clinical colorectal cancer patient indicates short lifetime of the patient and poor sensitivity of the patient to postoperative chemotherapy, and miR-150 high expression level indicates long lifetime of the patient and good sensitivity of the patient to postoperative chemotherapy. Compounds having high sensitivity to miR-150 can be screened by the method, and the compounds can be used for developing a kit for detecting the colorectal cancer. The kit is composed of compounds sensitive to the molecule miR-150 as active ingredients and a medicinal carrier. Simple detection of the miR-150 expression level is advantageous for monitoring of the colorectal cancer patient after healing and determination of chemotherapy sensitivity of the patient, and thus provides an effective method for clinical prevention and treatment of the colorectal cancer; high clinical application value and social benefit are created.

The invention relates to an Na+ / K+ ATPase inhibitor for use in the prevention or treatment of metastasis in a cancer patient defined by the presence of CTC clusters in the bloodstream. In certain embodiments the Na+ / K+ ATPase is a cardiac glycoside and is selected from: digitoxin, ouabain, convallatoxin, proscillaridin, lanatoside C, gitoformate, peruvoside, strophanthidin, metildigoxin, deslanoside, bufalin, digoxin and digoxigenin. The invention further relates to the use of nucleic acid agents inhibiting the expression of genes related to CTC cluster formation and maintenance.

Isolated polypeptides with steroid binding activity and methods for their use as therapeutics and detection agents are disclosed herein.

The invention discloses a compound polymerase chain-ELSA reaction prawn virus detecting method. Its steps: 1. design and synthesis of catch probe and amplifying primer; 2. extraction of tissue total nucleic acid; 3. labeling digoxigenin with compound polymerase chain reaction; 4. solid-phase cross; 5. enzyme linked coloration. It is adapted to diagnosis of white spot syndrome virus, taola syndrome virus and hepatopancreatic minute virus and monitoring of the health state of the prawn.

The invention mainly relates to the technical field of planting and discloses a pregermination method for fragrant-flowered garlic seeds. The pregermination method comprises the following steps of soaking, dressing seeds, performing pregermination and sowing. The method is simple and can carry out batch treatment; the pregermination time is shortened by 2 to 3 days; the seeds can germinate to unearth after the seeds are sown for 2 to 3 days, the germination rate of the fragrant-flowered garlic seeds is 97.5 percent, the planting efficiency of fragrant-flowered garlic is remarkably improved, and the planting cost is reduced; the fragrant-flowered garlic seeds are putted into a low-temperature soaking solution for soaking; abortive grains are removed, and seed coats are softened; the soaking solution is rich in multiple components and can provide nutrition; the activity of dormant substances is reduced, seed embryos are promoted to be germinated, the germinating time is shortened, and an application method of digoxigenin is added.

The invention provides bioconjugates of heterocylic compounds such as S-adenosylmethionine and S-adenosylhomocysteine with biotin or digoxigenin. The bioconjugates also include carbon and nitrogen linker moieties of varying length that are used to attach such compounds to biotin or digoxigenin. The conjugates are useful in immunoassays. The invention provides a method for detecting SAM and SAH, comprising the steps of: (a) preparing the following components: (i) bio-conjugates of SAM, SAM analogs or SAH; (ii) an europium, a terbium cryptate or other fluorophore as a donor that has a specific ligand for the tracer in the bio-conjugates of (i); (iii) an acceptor fluorescent dye that has the excitation spectra overlap those of donor's emissions and has an antibody specific for SAM or SAH labeled; (b) addition of the biological fluid containing said SAM or SAH; and (c) spectroscopic measurement of the fluorescence of the donor and the fluorescence of from the acceptor.

Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

The invention belongs to the technical field of animal pathogen molecule detection, and particularly relates to a detection primer, a detection kit and a virus detection method for a fowl adenovirus-I, and application of the primer. A specific primer is used for synthesizing a digoxigenin-labeled specific nucleic acid probe through PCR (Polymerase Chain Reaction). Through dot blot, the specific probe can be used for detecting the existence of 12 types of serotype nucleic acids of the fowl adenovirus-I in a pathological material sample, and is used for judging whether fowl adenovirus-I infection is in the presence or not. By use of the kit to which the invention relates, the DNA (deoxyribonucleic acid) of a pathological material tissue sample is extracted to carry out the dot blot, detection can be finished in 24-36h, a result is reported, so that specificity is high, and accuracy is high.

The invention relates to the field of marine biotechnology, and discloses a rapid screening method for anemone toxin-producing microorganisms in view of the technical gap of a rapid screening method for anemone toxin-producing microorganisms, comprising the following steps: (1) obtaining strain DNA; ( 2) Obtain DNA probe hybridization solution: put the digoxigenin-labeled DNA probe solution in boiling water and then put it in an ice bath, take the DNA probe solution and add it to the hybridization solution; (3) Hybridization: place the DNA membrane Place in DNA probe hybridization solution and incubate for hybridization; place the hybridized DNA membrane in a container containing 2 x SSC solution for washing; (4) develop color; (5) judge the result. The DNA probe-colony in situ hybridization method is used for the rapid screening of sea anemone toxin-producing microbial strains, and the strains that can produce sea anemone toxin can be accurately screened through specific amplified DNA probes and simple and orderly operation steps , this method is simple and efficient, with short screening period and high accuracy.

The invention discloses a kit for determining the sex of a sheep early embryo. The kit disclosed by the invention is provided with two pairs of specific primers, two probes and colloidal gold test strips, wherein the two pairs of specific primers are respectively SRY gene primers and interior label Callipyge gene primers, and the two probes are respectively an SRY probe and a Callipyge probe; each colloidal gold test strip is composed of a conjugate pad and a reaction pad; the conjugate pad is formed by coating colloidal gold on a BIOTIN antibody; the reaction pad is connected with the tail end of the colloidal gold conjugate pad, composed of a nitrocellulose membrane and respectively coated with a detection area and a quality control area; and the detection area is coated with an FITC (fluoresceinisothiocyanate) antibody, and the quality control area is positioned downstream from the detection area and coated with a DIG (digoxigenin) antibody.

The invention belongs to the technical field of biology and particularly relates to a detection technology combining multi-cross displacement amplification with gold nano detection and application ofthe detection technology in gene detection. According to the detection technology combining multi-cross displacement amplification with gold nano detection, by amplifying a label labelled at the 5' end of a primer C1 in multi-cross displacement amplification, the method is directed to the feature that amplification products of a specific gene pgaD (labeling isothiocyanate fluorescein FITC) of acinetobacter baumannii and a carbapenem drug resistance gene blaOXA-23-like (labeling digoxigenin Dig) can be visually detected by a gold nano-biosensor; and the method is convenient, fast, sensitive andspecific and is suitable for being popularized and applied to detection of various specific nucleotide fragments and being applied to detection of pathogenic bacteria corresponding to the specific nucleotide fragments.

The invention belongs to the technical field of detection of animal pathogeny molecules, and particularly relates to a detection primer, a detection reagent kit and a detection method for multiple viruses of parrots, and an application. According to the reagent kit disclosed by the invention, a CP gene for psittacine beak and feather disease viruses, a VP1 gene for budgerigar fledgling disease viruses, and a hexon gene for avian adenovirus are used as formworks, a specific primer is used, through PCR, specific nucleic acid probes are marked through digoxigenin, through dot blot, the probes canbe used for detecting the existence of psittacine beak and feather disease viruses, the budgerigar fledgling disease viruses or avian adenovirus nucleic acid in pathologic material samples. Through the utilization of the reagent kit, DNA in pathologic material tissue samples is extracted to be subjected to dot blot, detection can be completed in 24h-36h, the result is reported, the specificity ishigh, and the accuracy is high. If nucleic acid extracts of the same sample are respectively dropped on three membranes, besides, corresponding nucleic acid probes are added, and three different viruses can be detected at the same time.

A method of utilizing active-targeting to screen anti-AIDS natural product includes doping biotin-dUTP and digoxigenin-dUTP into DNA randowly to form digoxigenin-biotin double labeled NDA, catching DNA by using anti-dogoxigenin antibody specificity, using fluorescent labeled chain avidin and biotin labeled NDA specificity to carry out detection, knowing amount of biotin in synthesized NDA according to intensity of fluorescence then deriving out activity of HIV-I reversed transcriptive enzyme.

The invention relates to the technical field of marine biology, in particular to a method for rapidly screening microbial strains producing tetrodotoxin and a digoxin-labeled DNA probe used therefor, the probe used in the method is 254 complementary to the DNA base to be tested Base-length single-stranded DNA, the 3' end of the probe is labeled with digoxigenin. Through colony in situ dot molecular hybridization, the probe can specifically detect whether there is tetrodotoxin synthesis sxt gene nucleic acid in the DNA sample of the strain to be tested, and screen out toxin-producing positive strains. The method involved in the invention utilizes the in situ extract of bacterial strain DNA to perform colony dot hybridization, and can complete the screening of 96 samples within 8-10 hours. The method of the invention is fast, has strong specificity and high accuracy.

The invention relates to the technical field of marine biology, in particular to a method for rapidly screening microbial strains producing gonitoxins and a digoxin-labeled DNA probe used therein. The probe used in the method is complementary to the DNA base to be tested 248 bases long single-stranded DNA, the 3' end of the probe is labeled with digoxigenin. Through colony in situ dot molecular hybridization, the probe can specifically detect whether there is sxtS gene nucleic acid in the DNA sample of the strain to be tested, and screen out toxin-producing positive strains. The method involved in the invention utilizes the in situ extract of bacterial strain DNA to perform colony dot hybridization, and can complete the screening of 96 samples within 8-10 hours. The method of the invention is fast, has strong specificity and high accuracy.