35 results about "Digoxigenin" patented technology

The preparation of vitamin D compounds of formula (I) with a label attached to a spacer group in the 3 position is disclosed.In the above formula (I), O represents the oxygen atom of an ether group; Y represents hydrogen or hydroxy; A represents a label such as biotin, digoxigenin, or another vitamin D group; R represents a substituted hydrocarbon side-group of vitamin D or a vitamin D metabolite. Also disclosed is a method of measuring 25-hydroxy vitamin D metabolite and a 1α,25-dihydroxy vitamin D metabolite in a sample.

The present invention relates to complexes of a) bi-specific antibodies and antibody fragments against a target protein and b) a digoxigenin conjugated to a therapeutic or diagnostic agent, methods for their production, their use as a delivery platform for therapeutic or diagnostic agents, pharmaceutical compositions containing said antibodies, and uses thereof.

The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below.The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.

Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

The invention discloses a compound polymerase chain-ELSA reaction prawn virus detecting method. Its steps: 1. design and synthesis of catch probe and amplifying primer; 2. extraction of tissue total nucleic acid; 3. labeling digoxigenin with compound polymerase chain reaction; 4. solid-phase cross; 5. enzyme linked coloration. It is adapted to diagnosis of white spot syndrome virus, taola syndrome virus and hepatopancreatic minute virus and monitoring of the health state of the prawn.

The invention mainly relates to the technical field of planting and discloses a pregermination method for fragrant-flowered garlic seeds. The pregermination method comprises the following steps of soaking, dressing seeds, performing pregermination and sowing. The method is simple and can carry out batch treatment; the pregermination time is shortened by 2 to 3 days; the seeds can germinate to unearth after the seeds are sown for 2 to 3 days, the germination rate of the fragrant-flowered garlic seeds is 97.5 percent, the planting efficiency of fragrant-flowered garlic is remarkably improved, and the planting cost is reduced; the fragrant-flowered garlic seeds are putted into a low-temperature soaking solution for soaking; abortive grains are removed, and seed coats are softened; the soaking solution is rich in multiple components and can provide nutrition; the activity of dormant substances is reduced, seed embryos are promoted to be germinated, the germinating time is shortened, and an application method of digoxigenin is added.

The invention relates to the field of marine biotechnology, and discloses a rapid screening method for anemone toxin-producing microorganisms in view of the technical gap of a rapid screening method for anemone toxin-producing microorganisms, comprising the following steps: (1) obtaining strain DNA; ( 2) Obtain DNA probe hybridization solution: put the digoxigenin-labeled DNA probe solution in boiling water and then put it in an ice bath, take the DNA probe solution and add it to the hybridization solution; (3) Hybridization: place the DNA membrane Place in DNA probe hybridization solution and incubate for hybridization; place the hybridized DNA membrane in a container containing 2 x SSC solution for washing; (4) develop color; (5) judge the result. The DNA probe-colony in situ hybridization method is used for the rapid screening of sea anemone toxin-producing microbial strains, and the strains that can produce sea anemone toxin can be accurately screened through specific amplified DNA probes and simple and orderly operation steps , this method is simple and efficient, with short screening period and high accuracy.

The invention discloses a method for detecting miRNAs by a DIG labeling EDC (carbodiimide) cross-linking bridging method. Once a DIG labeling system is utilized, the sensitivity of the DIG labeling system is improved by at least 50 times in contrast with that of a traditional DIG labeling Northern blots detection system; and the sensitivity of the DIG labeling system is equivalent with that of a radioisotope labeling Northern blots detection system; the method cannot cause any pollution on environment, and is very safe and free of a hybridization step; all operations can be completed in 9-10 hours; and therefore, the method is convenient, quick, and low in detection cost. As a result, the problems of low safety, complex procedure, high price, low sensitivity and the like of a current detection method are solved.

The invention discloses a kit for determining the sex of a sheep early embryo. The kit disclosed by the invention is provided with two pairs of specific primers, two probes and colloidal gold test strips, wherein the two pairs of specific primers are respectively SRY gene primers and interior label Callipyge gene primers, and the two probes are respectively an SRY probe and a Callipyge probe; each colloidal gold test strip is composed of a conjugate pad and a reaction pad; the conjugate pad is formed by coating colloidal gold on a BIOTIN antibody; the reaction pad is connected with the tail end of the colloidal gold conjugate pad, composed of a nitrocellulose membrane and respectively coated with a detection area and a quality control area; and the detection area is coated with an FITC (fluoresceinisothiocyanate) antibody, and the quality control area is positioned downstream from the detection area and coated with a DIG (digoxigenin) antibody.

Isolated polypeptides with steroid binding activity and methods for their use as therapeutics and detection agents are disclosed herein.

The invention belongs to the technical field of detection of animal pathogeny molecules, and particularly relates to a detection primer, a detection reagent kit and a detection method for multiple viruses of parrots, and an application. According to the reagent kit disclosed by the invention, a CP gene for psittacine beak and feather disease viruses, a VP1 gene for budgerigar fledgling disease viruses, and a hexon gene for avian adenovirus are used as formworks, a specific primer is used, through PCR, specific nucleic acid probes are marked through digoxigenin, through dot blot, the probes canbe used for detecting the existence of psittacine beak and feather disease viruses, the budgerigar fledgling disease viruses or avian adenovirus nucleic acid in pathologic material samples. Through the utilization of the reagent kit, DNA in pathologic material tissue samples is extracted to be subjected to dot blot, detection can be completed in 24h-36h, the result is reported, the specificity ishigh, and the accuracy is high. If nucleic acid extracts of the same sample are respectively dropped on three membranes, besides, corresponding nucleic acid probes are added, and three different viruses can be detected at the same time.

A method of utilizing active-targeting to screen anti-AIDS natural product includes doping biotin-dUTP and digoxigenin-dUTP into DNA randowly to form digoxigenin-biotin double labeled NDA, catching DNA by using anti-dogoxigenin antibody specificity, using fluorescent labeled chain avidin and biotin labeled NDA specificity to carry out detection, knowing amount of biotin in synthesized NDA according to intensity of fluorescence then deriving out activity of HIV-I reversed transcriptive enzyme.

The invention relates to a wheat yellow streak virus and a NASH detection method and detection kit thereof. The present invention discovers a new virus, namely wheat yellow streak virus WYSV, the full-length sequence of which is shown in SEQ ID NO. , using digoxigenin as a marker to prepare DNA probes to detect WYSV virus. The invention also provides a WYSV virus kit for NASH detection, the kit includes: a probe PCR reaction solution, a NASH reaction reagent, a nitrocellulose membrane, a hybridization bag, a WYSV positive control sample and a negative control sample. The detection method and detection kit can detect samples in batches at one time, can detect WYSV virus in a short time with low cost and high efficiency, and have reliable detection results, easy operation, strong specificity, high sensitivity, short time consumption, and good application prospect.

The invention relates to the technical field of marine biology, in particular to a method for rapidly screening microbial strains producing tetrodotoxin and a digoxin-labeled DNA probe used therefor, the probe used in the method is 254 complementary to the DNA base to be tested Base-length single-stranded DNA, the 3' end of the probe is labeled with digoxigenin. Through colony in situ dot molecular hybridization, the probe can specifically detect whether there is tetrodotoxin synthesis sxt gene nucleic acid in the DNA sample of the strain to be tested, and screen out toxin-producing positive strains. The method involved in the invention utilizes the in situ extract of bacterial strain DNA to perform colony dot hybridization, and can complete the screening of 96 samples within 8-10 hours. The method of the invention is fast, has strong specificity and high accuracy.

The invention provides a method for labeling in situ hybridization centromere probes with digoxin, and belongs to the technical field of nucleic acid labeling in molecular biology. The method of using digoxigenin to label in situ hybridization centromere probes is mainly used to label digoxigenin on centromere probes, and then the centromere probes can be applied to FISH probes to detect centromere purpose of change. The method of using digoxigenin to label in situ hybridization centromere probes provided by the invention can effectively label digoxigenin molecules on oligonucleotides, and can be applied to the research of molecular markers and clinical molecular diagnosis.

The invention relates to the technical field of marine biology, in particular to a method for rapidly screening microbial strains producing gonitoxins and a digoxin-labeled DNA probe used therein. The probe used in the method is complementary to the DNA base to be tested 248 bases long single-stranded DNA, the 3' end of the probe is labeled with digoxigenin. Through colony in situ dot molecular hybridization, the probe can specifically detect whether there is sxtS gene nucleic acid in the DNA sample of the strain to be tested, and screen out toxin-producing positive strains. The method involved in the invention utilizes the in situ extract of bacterial strain DNA to perform colony dot hybridization, and can complete the screening of 96 samples within 8-10 hours. The method of the invention is fast, has strong specificity and high accuracy.