The invention discloses an
autoimmunity detection kit with
enzyme-linked immuno spot blotting and an operation process thereof. The
autoimmunity detection kit with the
enzyme-linked immuno spot blotting comprises a kit body, target molecules and a
reagent. The kit is a technology based on combination of
enzyme-linked immuno spot blotting and enrichment and is used for detecting pathoglycemia caused by deficiency of the
autoimmunity function. The operation process of the autoimmunity detection kit with the enzyme-linked immuno spot blotting comprises the following steps of: preparing the targetmolecules, preparing a covering device of the target molecules, preparing
cell lysis solution, preparing anti-human immune
cell antibodies, enriching the immune cells, preparing amplification culturemedium, detecting the target molecules and analyzing clinical-sample detection data. The autoimmunity detection kit and the operation process disclosed by the invention have the beneficial effects that by combination of the immune
cell enrichment technology and the enzyme-linked immuno spot blotting technology with high sensitivity and high specificity and accurate analysis for the correspondingtarget molecules, the detection specificity is improved and the false positive probability is reduced.