Detection primer, detection kit and virus detection method for fowl adenovirus-I, and application of primer
A technology of avian adenovirus and detection primers, which is applied in the field of detection primers for group I avian adenoviruses, and can solve problems such as non-specificity and limitations of detection methods.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0112] Embodiment 1: the sensitivity test of kit of the present invention detects FAdV-Ⅰ
[0113] (1) Sample: FAdV-I non-labeled positive control nucleic acid.
[0114] (2) Sample treatment: Dilute the FAdV-I non-labeled positive control nucleic acid to 1000, 100, 10, 1, 0.1 pg / μl, and negative control nucleic acid on the nylon membrane in sequence, mark and perform dot hybridization .
[0115] (3) Use tweezers to place the nylon membrane (with the sampling side up) on the filter paper saturated with denaturing solution (the filter paper should be placed in a glass plate in advance) for denaturation for 10 minutes, then take it out with tweezers and place it in the filter paper saturated with neutralizing solution. Neutralize for 5 minutes on a filter paper (the filter paper is placed in a glass plate in advance).
[0116] (4) Take out the film with tweezers, put it on a plate (with the sample point facing up) and dry it at room temperature for 10 minutes until there are no ...
Embodiment 2
[0140] Embodiment 2: The kit of the present invention detects the specificity test of FAdV-Ⅰ
[0141] (1) Samples: Avian Reticuloendothelioma Virus (REV) Genomic DNA, Chicken Marek's Disease Virus (MDV) Genomic DNA, Chicken Infectious Anemia Virus (CIAV) Genomic DNA, Chicken Leukemia Virus (ALV) Genomic DNA, Chicken Reovirus (ARV) Genomic DNA, Chicken Infectious Bronchitis Virus (IBV) Genomic DNA, Parrot Beaking Disease Virus (PBFDV) Genomic DNA, Avian Polyoma Virus (APV) Genomic DNA DNA.
[0142] (2) Sample processing: Take 1 μl of the negative control nucleic acid and positive control nucleic acid in the kit and 2 μl of the genomic DNA of REV, MDV, CIAV, ALV, ARV, IBV, PBFDV and APV respectively, and spot them on the nylon membrane respectively. on, mark it.
[0143] (3) Use tweezers to place the nylon membrane (with the sampling side up) on the filter paper saturated with denaturing solution (the filter paper should be placed in a glass plate in advance) for denaturation ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com