93 results about "Fowl adenovirus" patented technology

The invention provides a group I FAdV-4 (fowl adenovirus serotype 4) vaccine. An antigen of the group I FAdV-4 vaccine is an inactivated YBAV-4 virus. According to the prepared group I FAdV-4 inactivated vaccine, the immune effect of the vaccine is evaluated with a serology method and a vaccinated challenge method, a result shows that the prepared fowl adenovirus inactivated vaccine can provide effective immune protection for fowls, and the group I FAdV-4 inactivated vaccine has a good commercial development prospect.

The invention provides a combined inactivated vaccine for an avian influenza virus and a fowl adenovirus. An H9 subtype avian influenza virus QDY strain and an I-group 4-type aviadenovirus YBAV-4 new strain used by the vaccine are high in TCID50 / EID50 valence and good in immunogenicity and can well withstand attacks of the H9 avian virus and various local separate viruses. The vaccine prepared by the invention is good in safety, and free of local and whole-body adverse effects caused by the vaccine. Through analysis of characters, a safety test and efficacy test data in a storage-life test, the combined vaccine is free of an obvious difference from a single vaccine of a similar product in effect, and is stable and effective; the efficacy test result proves that the antibodies of the combined vaccine and two single vaccines are kept at a high level and are faster in antibody generation of similar products; and a control group antibody is negative.

The invention discloses an F2 protein based indirect ELISA kit for detection of fowl adenovirus serotype 4 (FAdV-4) antibody. The kit includes an ELISA plate coated with a spike protein gene expression product, a positive and negative control, an HRP labeled rabbit anti-chicken secondary antibody, a sample diluent, a color development liquid and a washing liquid. The indirect ELISA kit for FAdV-4antibody established by the invention can specifically detect the FAdV-4 antibody, and does not react with anti-influenza virus (AIV) serum, anti-mardivirus (MDV) serum, anti-Newcastle disease virus (NDV) serum, anti-avian reticuloendotheliosis virus (REV) serum and SPF chicken serum (Figure 6). Therefore, the kit has good FAdV-4 specificity, and can be used for FAdV-4 infection and immune statusepidemiological investigation.

The invention relates to a PCR detection kit for specific detection of fowl adenovirus group I and a detection method thereof. The detection kit comprises a fowl adenovirus group I PCR reaction liquid, 10*PCR buffer for UNG plus, hot start type DNA polymerase, dU plus dNTP mix, uracil DNA glycosylase, positive quality control serum and negative quality control serum, wherein the fowl adenovirus group I PCR reaction liquid comprises an upstream primer with a concentration of 5 micrometer and the sequence is shown as the specifications as well as a downstream primer with a concentration of 5 micrometer and the sequence is shown as the specifications. When detection is conducted through the PCR detection kit, conditions for a PCR amplified reaction are that UNG treatment is conducted at a temperature of 25 DEG C for 10 min, and predegeneration is conducted at a temperature of 95 DEG C for 2 min; degeneration is conducted at a temperature of 98 DEG C for 10 s, annealing is conducted at a temperature of 55 DEG C, extension is conducted at a temperature of 72 DEG C for 1 min, and all that are conducted for 30 work cycling; extension is conducted at a temperature of 72 DEG C for 10 min. The PCR detection kit for the specific detection of the fowl adenovirus group I has the advantages of being high in sensitivity (still positive when the reaction liquid is diluted to 1:105) and good in repeatability. The PCR detection method for the specific detection of the fowl adenovirus group I is easy, convenient and fast to operate, suitable for early diagnosis of fowl adenovirus group I virus, and can meet the demand for disease prevention and control in time.

The invention provides a genetically-engineered vaccine against fowl adenovirus type 4, and a preparation method and application thereof. According to the invention, protective antigens, namely pentonand hexon proteins of fowl adenovirus serotype 4 are connected by a linker, and are expressed by an insect cell-baculovirus expression system to form virus-like particles of fowl adenovirus type 4 onspatial conformation; an adjuvant is added; and emulsification is carried out so as to prepare the genetically-engineered vaccine against fowl adenovirus type 4. The preparation method for the vaccine provided by the invention is simple in process, capable of preparing a large amount of fowl adenovirus type 4 antigen protein, short in time consumption, high in expression quantity and beneficial to large-scale production; and the obtained genetically-engineered vaccine is good in immune effect and capable of effectively preventing infection of the fowl adenovirus type 4.

The invention relates to a heat-resistant serum 4 type fowl adenovirus genetic engineering vaccine candidate strain and a construction method thereof. The candidate strain is a thermal-stability newcastle disease virus (Newcastle Disease Virus) rAHR09-4F2 expressing serum 4 type fowl adenovirus spike protein 2, and the preservation number is CCTCC NO: V201932. The candidate strain is obtained through using a heat-resistant newcastle disease attenuated virus strain rAHR09 as a carrier, inserting a serum 4 type fowl adenovirus (FAdV4) spike protein (fiber2) gene between a P intergenic region andan M intergenic region, and adopting a backward heredity technique. The result of evaluating the biological characteristics and the immunoprotection efficacy of the candidate strain displays that therecombinant virus has favorable heat stability and immunogenicity and provides favorable immunoprotection on FAdV4 infection.

The invention discloses a primer group and a kit for detecting a fowl adenovirus serotype 4 (FAdV-4) as well as a detection method and an application of the primer group. The primer group comprises outer primers and inner primers, wherein the outer primers include F3 and B3, the inner primers include FIP and BIP, the nucleotide sequence of the F3 is shown as SEQ ID NO.1, and the nucleotide sequence of the B3 is shown as SEQ ID NO.2; and the nucleotide sequence of the FIP is shown as SEQ ID NO.3, the nucleotide sequence of the BIP is shown as SEQ ID NO.4, the FIP is a 5-end biotin labeled sequence, and the BIP is a 5-end fluorescein isothiocyanate labeled sequence. By combination of a loop-mediated isothermal amplification (LAMP) technology with a lateral flow dipstick (LFD) technology, a set of primers are designed for an important factor 52 k gene of the FAdV-4, and an LAMP-LFD rapid detection technology of the FAdV-4 is established. The LAMP-LFD kit provided by the invention is convenient to operate, safe, reliable, sensitive, efficient, good in specificity and particularly suitable for field rapid visual detection.