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Group I FAdV-4 (fowl adenovirus serotype 4) vaccine

A poultry adenovirus and virus technology, applied in antiviral agents, antibody medical ingredients, medical preparations containing active ingredients, etc., can solve the problems of loopholes in the prevention and control of group I poultry adenoviruses, and achieve good commercial development prospects, The effect of effective immune protection

Active Publication Date: 2016-03-23
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the prevention and control of this disease, there are commercial vaccines against group I adenovirus in foreign countries, but there is no vaccine available in China so far, which leads to loopholes in the prevention and control of group I avian adenovirus

Method used

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  • Group I FAdV-4 (fowl adenovirus serotype 4) vaccine
  • Group I FAdV-4 (fowl adenovirus serotype 4) vaccine
  • Group I FAdV-4 (fowl adenovirus serotype 4) vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Isolation and Identification of YBAV-4 Strain

[0013] 1. Epidemiological survey Since 2010, some broiler breeders, laying hens and Ma chickens in Shandong, Jiangsu and other regions have experienced a disease characterized by high mortality and anatomical manifestations of hepatomegaly and hydropericardium. The disease was initially diagnosed as hydropericardium hepatitis syndrome caused by group I group C-4 avian adenovirus through clinical investigation and laboratory testing. In 2010, the inventor successfully isolated a virus strain from the liver of a dead chicken with typical symptoms of inclusion body hepatitis and hydropericardium in a farm in Zibo, Shandong.

[0014] 2. Virus isolation Take the liver of sick and dead chicken and grind it, add sterilized saline at a ratio of 1:5 to make a suspension; after repeated freezing and thawing 3 times, centrifuge at 3000r / min for 30min, take the supernatant; add penicillin and Streptomycin at 10,000 IU / ml each, overni...

Embodiment 2

[0036] Preparation of YBAV-4 Strain Virus Seeds

[0037] Select well-growing chicken hepatocytes, discard the original culture medium, add maintenance solution containing 1% virus seed (the third generation of the original virus seed of YBAV-4 strain), and culture at 37°C for 36-48 hours. % above, harvested, frozen and thawed twice, packed in sterilized containers, and sampled for identification. Indicate the date of harvest, generation of poisonous seeds, etc. According to this method, 15 generations were passed continuously, which were respectively marked as C4-C18 generations. Virus content was determined at each passage. Will test for sterility and virus content ≥10 7.0 TCID 50 The virus liquid is mixed, quantitatively divided, and stored in a freeze-dried manner. Indicate the date of harvest, generation of poisonous seeds, etc.

Embodiment 3

[0039] Preparation of virus solution for seedling production of YBAV-4 strain

[0040] 1 Seedling preparation material selection Group I Group 4 poultry adenovirus liquid preparation, select chicken liver cells.

[0041] 2 Cell preparation Take out the cryopreservation tube from the liquid nitrogen tank and put it in a 37°C water bath to thaw, transfer the cells into a centrifuge tube containing 10ml of culture medium, and centrifuge at 1000r / min for 5 minutes. Suspend cells in culture medium containing 20% ​​newborn bovine serum, place at 37°C, 5% CO 2 Cultivate in an incubator, digest cells with trypsin-EDTA when they grow into a good monolayer, and subculture with cell nutrient solution.

[0042] 3. Cell monolayer culture Inoculate the expanded cultured seed cells into spinner bottles and culture at 37°C.

[0043] 4. Select chicken hepatocytes with good growth for poisoning, discard the original culture medium, add maintenance solution containing 1% virus species, and conti...

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Abstract

The invention provides a group I FAdV-4 (fowl adenovirus serotype 4) vaccine. An antigen of the group I FAdV-4 vaccine is an inactivated YBAV-4 virus. According to the prepared group I FAdV-4 inactivated vaccine, the immune effect of the vaccine is evaluated with a serology method and a vaccinated challenge method, a result shows that the prepared fowl adenovirus inactivated vaccine can provide effective immune protection for fowls, and the group I FAdV-4 inactivated vaccine has a good commercial development prospect.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, in particular to a group I type 4 adenovirus vaccine. technical background [0002] Group I avian adenoviruses make up the genus Avian Adenovirus in the family Adenoviridae. Group II (Turkey Hemorrhagic Enteritis and Related Viruses) and Group III (Egg Drop Syndrome) adenoviruses are clearly associated with disease, in contrast to most Group I avian adenoviruses that have not been shown to be pathogenic in poultry. totally sure. But FAdV-1 and FAdV-4 are obviously exceptions, among which FAdV-1 can cause quail bronchitis, and FAdV-4 is the main cause of hydropericardium hepatitis syndrome. Other adenovirus strains allow rapid infection of opportunistic pathogens when chicken health is compromised, such as co-infection with other pathogens such as Chicken Infectious Anemia Virus (CIAV) and Infectious Bursal Disease Virus (IBDV) . Group I poultry adenoviruses have identified five po...

Claims

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Application Information

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IPC IPC(8): A61K39/235A61P31/14
CPCA61K39/235A61K2039/5252A61K2039/55511
Inventor 王红申茂欣韩建文郑长虹刘蕾窦小龙夏娜赵同渊马爽宋新宇
Owner YEBIO BIOENG OF QINGDAO
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