Vaccine of encephalomyelitis of poultry
A technology for avian encephalomyelitis and avian brain, applied in the direction of antibody medical ingredients, medical preparations containing active ingredients, antiviral agents, etc., to achieve good commercial development prospects, effective immune protection, and genetically stable effects
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Embodiment 1
[0015] Embodiment 1: Breeding of avian encephalomyelitis virus strain
[0016] Take the brain, pancreas and duodenum of chicks with avian encephalomyelitis, grind them under sterile conditions, dilute them with sterilized normal saline at a ratio of 1:5, repeat freezing and thawing three times, centrifuge at 4000rpm for 30 minutes, and take the supernatant After filtering through the liquid filter, 10 1-day-old SPF chicks were inoculated in the brain, 0.1ml per chick. After symptoms such as ataxia and head and neck tremors appeared, blood was collected to kill them. Grind under sterile conditions, dilute 1:5 with sterilized saline, freeze-thaw 3 times, centrifuge at 4000rpm for 30 minutes, take the supernatant as the first-generation isolated virus (F1) and save it for later use. The F1 generation was inoculated into the brain of 1-day-old SPF chicks by the same method as above, and the second generation of isolated virus (F2) was prepared. This has been passed on for 4 gener...
Embodiment 2
[0027] Preparation and application of embodiment 2 poultry cerebrospinal live vaccine
[0028] 1 material
[0029] 1.1 Virus Seed The attenuated YBF02 strain isolated from CGMCC No. 8505 was selected as the avian encephalomyelitis virus used for vaccine production.
[0030] 1.2 Virus for inspection The virulent VR strain of avian encephalomyelitis virus for inspection is identified, preserved and supplied by the China Veterinary Drug Control Institute.
[0031] 1.3 Breeding eggs and test chickens
[0032] 1.3.1 SPF eggs were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.
[0033] 1.3.2 7-14-day-old SPF chickens SPF eggs were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd., hatched by themselves after purchase, and reared in a negative pressure isolator after hatching.
[0034] 1.4 Other instruments and reagents for seedling production were provided by Qingdao Yibang Bioengineering Co., Ltd.
[0035] 2 methods
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