355results about How to "Genetically stable" patented technology

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g / l.

The invention relates to a method for breeding a novel bee species by breeding a hybrid of east bees and west bees, comprising introducing a west bee brood comb going to come out cells into a swarm of east bees preparing for laying eggs, to make the number of east worker bees equal to that of west worker bees, and obtaning a mixed swarm; the east queen bee laying eggs on empty combs, moving out the east queen bee to make the swarm without a queen, after the first generation east worker bees have got out cells; putting in a queen cell frame 20-22 hours later, breeding a queen with four-day old east bee grubs of same swarm to get a novel east queen bee; and hybridizing said queen as mother with west male bee as father by artificial insemination or natural copulation to obtain a hybrid thereof. The method breaks the reproductive isolation between two different species, and the hybrid obtained thereby has a remarkble heterosis.

The invention relates to a preparation method of Muscovy duck parvo novel vaccines, which comprises the following steps of: using Muscovy duck parvovirus attenuated virus P1 strain (MPV-P1 strain with a preservation number of CCTCC NO:V201013) and Muscovy duck origin goose parvovirus attenuated virus D strain ( GPV-D strain with a preservation number of CCTCC NO:V201014) as seed viruses; proliferating viruses by applying the muscovy duck embryone fibroblast spinner culture technology to obtain cell toxic liquids; mixing the cell toxic liquids according to the proper proportion and freezing a dry protective agent to freeze and dry to research safe and effective Muscovy duck parvo novel vaccines. The young Muscovy ducks are inoculated once so as to prevent and control the Muscovy duck parvovirus infection and the Muscovy duck gosling blast dieases at the same time, thereby the stress reaction of the Muscovy ducks caused by immunization for many times is solved. The invention is suitable for scale production under the GMP (Good Manufacturing Practices) condition, and saves the production cost of the vaccines.

The invention discloses debaryomyces hansenii as well as a fermentation fluid, a mixed fermentation fluid and application thereof to secondary fermentation of vegetables, wherein the debaryomyces hansenii is (Debaryomyces hansenii) W08 with preservation number of CGMCC No.5770. The invention also discloses a debaryomyces hansenii fermentation fluid which is obtained by inoculating the debaryomyces hansenii in a PDA fluid medium to culture. The invention also discloses a mixed fluid which comprises the debaryomyces hansenii fermentation fluid, a lactobacillus plantarum fermentation fluid and a leuconostoc mesenteroides fermentation fluid; the mixed fermentation fluid is applied to the secondary fermentation of vegetables, and has the effects of forming special micro-ecological environment and accelerating the forming of more substances with favorable flavor of vegetables to improve the edibility.

The invention relates to a slaughter type high-quality chilling fresh chicken breeding method. The breeding method includes: selecting recessive white-feather chickens (B line) having green shins and yellow skins as first male parents, and selecting large cockscomb high yield chickens (C line) having yellow shins and yellow skins as first female parents; hybridizing the B line and the C line to acquire parental generations (BC system) to be as final female parents according to the heredity principle that a green shin gene is sex-linked and an autosome controls a skin color gene, wherein parental chickens can be distinguished through the shin color, the shins of the female chickens are green, and the shins of the male chickens are yellow. using yellow-feather fast-growing chickens (A line) having green shins, yellow skins, and large cockscombs as final male parents, producing commodity chickens (ABC) by A line male X (B line male X C line female) female mating hybrid, wherein commodity male and female chickens have green shins, yellow skins, large cockscombs, and wide chests. The commodity chickens can be sold at the age of 63-65 days, the average weight of the female chickens is 1.6-1.65 Kg, the average weight of the male chickens is 1.8-1.85 Kg, the average uniformity can reach more than 96%; the dressing percentage, the whole net carcass rate, and the chest muscle rate can exceed 85%, 65%, and 18%, and the carcass character has significant advantages.

The invention relates to a porcine reproductive and respiratory syndrome virus chimeric recombinant vaccine strain and a method for producing living vaccine with same; latter half part of GP2 gene and GP3-GP5 gene of the porcine reproductive and respiratory syndrome virus (PRRSV) classic virus PTK strain, M ferritin, N ferritin and spacer sequences thereof and all nucleotide sequences of 3' non-coding region are all deleted by using reverse genetic manipulation and gene combination technology, then replaced with corresponding gene of Chinese highly pathogenic porcine reproductive and respiratory syndrome variant strain and are recombined to be the chimeric recombinant vaccine strain (PC strain) with the PRRSV classic strain and variant strain genes simultaneously, the chimeric recombinant vaccine PC strain is used to produce the living vaccine which has low toxicity, ensures no returning of strong toxicity within 5 generations of returning swine and the heritability is stable; the invention has good immunogenicity, and can prevent the porcine reproductive and respiratory syndrome virus classic strain and highly pathogenic variant strain from being infected simultaneously.

The invention discloses a docosahexaenoic acid-producing bacterium and a mutation and screening method and application thereof. The strain is classified and named as Crypthecodinium cohnii HX-308, is preserved in China Center for Type Culture Collection, and has the preservation number M208246. The strain is a DHA-producing bacterium with high yield, has the DHA content 1.53 times of a starting strain, stable physiological-biochemical characteristic and stable heritability, and simultaneously can produce polyunsaturated fatty acid through liquid culture and fermentation; the content of unsaturated fatty acid reaches 20.4g / L, wherein the DHA content reaches 8.16g / L; and the strain is a good strain for producing natural DHA, and has excellent industrial production prospect.

The invention belongs to the field of pharmaceutic preparation, and relates to a targeted drug-carrying liposome of a tumor associated fibroblast. The surface of the targeted drug-carrying liposome is modified with a high-affinity ligand of a specific expression protein of the tumor associated fibroblast. The targeted drug-carrying liposome is capable of realizing targeted delivering of drugs into the tumor associated fibroblast, realizing intervention on tumor microenvironment via action on the tumor associated fibroblast, and realizing tumor treatment. And in addition, the tumor associated fibroblast targeted preparation can be combined with routine tumor cell targeted nano preparations so as to improve antitumous effects of the routine tumor cell targeted nano preparations.

The invention discloses a method for molecular inspection of cowpea variety based on analysis of genome RAPD. Extracting DNA of the available genome cowpea varieties, and generating separately polyase chain reaction to prepare the random augmentative polymorphic DNA product, then processing electrophoretic separation to obtain random augmentative polymorphic DNA standard atlas; analyzing the inspection-required cowpea variety following said steps to get the random augmentative polymorphic DNA atlas, comparing and deciding it with standard atlas, the inspection result is obtained. With the invention, it can definitely decide the different varieties of cowpea, and realize precise and rapid inspection of variety truth and variety purity, providing reliable technique of variety inspection, market control of seed, variety protection, quality control of pre-sowing seed, stock breeding and so on.

First generation adenoviral vectors and associated recombinant adenovirus-based HIV vaccines which show enhanced stability and growth properties and greater cellular-mediated immunity are described within this specification. These adenoviral vectors are utilized to generate and produce through cell culture various adenoviral-based HIV-1 vaccines which contain HIV-1 gag, HIV-1 pol and / or HIV-1 nef polynucleotide pharmaceutical products, and biologically relevant modifications thereof. These adenovirus vaccines, when directly introduced into living vertebrate tissue, preferably a mammalian host such as a human or a non-human mammal of commercial or domestic veterinary importance, express the HIV1-Gag, Pol and / or Nef protein or biologically modification thereof, inducing a cellular immune response which specifically recognizes HIV-1. The exemplified polynucleotides of the present invention are synthetic DNA molecules encoding HIV-1 Gag, encoding codon optimized HIV-1 Pol, derivatives of optimized HIV-1 Pol (including constructs wherein protease, reverse transcriptase, RNAse H and integrase activity of HIV-1 Pol is inactivated), HIV-1 Nef and derivatives of optimized HIV-1 Nef, including nef mutants which effect wild type characteristics of Nef, such as myristylation and down regulation of host CD4. The adenoviral vaccines of the present invention, when administered alone or in a combined modality regime, will offer a prophylactic advantage to previously uninfected individuals and / or provide a therapeutic effect by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of HIV-1 infection.

The invention provides a trichoderma reesei strain capable of producing cellulase with high yield through space mutation, belonging to the technical field of microorganism mutation. According to the invention, trichoderma reesei Rw-X1 strain is preferably selected to be carried by Shenzhou IX to be subjected to space mutation and trichoderma reesei TG-C521 strain capable of producing cellulase with high yield can be carefully screened and cultured; the strain is collected in China General Microbiological Culture Collection Center on August 25, 2014 and has a collection Number of CGMCCNo.9537. According to the invention, the genetic character of the strain is changed by virtue of a special environment so that a strain with normal distributed mutation and stable genetic transformation can be screened out preferably; and the strain is used for producing cellulose and the filter paper activity of the strain is 25.83-34.49% higher than that of the trichoderma reesei Rw-X1 strain; the strain is friendly to the environment and is free of pollution, thus having a wide industrial application prospect.

The invention relates to an attenuated vaccine strain of a VII type new castle disease virus with a mutated L gene which is produced by reverse genetic operation technology and a preparation method thereof, and the invention belongs to the fields of biological products and biological technology. The virulence of the attenuated vaccine strain of the VII type new castle disease virus with the mutated L gene is obviously decreased, simultaneously the attenuated vaccine strain has the characteristics of high titer, stable heritability and high matching with the antigen of the epidemic virus according to the chicken embryo experiment; the preparation method is suitable for large scale production of vaccines, and is used for producing vaccines; compared with the conventional vaccines (gene I and gene II types), the attenuated vaccine strain of the VII type new castle disease virus with the mutated L gene has wide application prospects in the aspects of morbidity and epidemic control of new castle disease.

The invention provides newly separated enterococcus faecium (enterococcus faecium) HEW-A588. The enterococcus faecium HEW-A588 has obvious probiotic and stress resistance. When the micro-ecologic preparation prepared from the enterococcus faecium enters intestinal tracts of animals, the micro-ecologic preparation is rapidly activated and proliferated, the micro-ecologic preparation can have the efficacies of forming beneficial advantage microbial populations, maintaining the balance of the intestinal tracts, improving the food utilization efficiency of the animals, reducing the cost, improving the steady state of the internal environment of the digestive tracts in the animal bodies, promoting the absorption and the utilization of nutrition, promoting the growth of the animals, improving economic benefits and the like, and the micro-ecologic preparation has favorable application prospects.

First generation adenoviral vectors and-recombinant adenovirus-based HIV vaccines which contain HIV-1 gag, HIV-1 pol and / or HIV-1 nef polynucleotide pharmaceutical products, and biologically relevant modifications thereof are described. The adenovirus vaccines, when directly introduced into living vertebrate tissue, express the relevant proteins, inducing a cellular immune response which specifically recognizes HIV-1. The exemplified polynucleotides of the present invention are synthetic DNA molecules encoding HIV-1 Gag, HIV-1 Pol, HIV-1 Nef, and derivatives thereof. The adenoviral vaccines of the present invention, alone or in combination, will offer a prophylactic advantage to previously uninfected individuals and / or provide a therapeutic effect by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of HIV-1 infection.

The invention belongs to the field of molecular genetics, and relates to molecular marker of heat stress resistant breeding of a Hostein cattle and application thereof. The molecular marker is SEQ ID NO.3. The molecular marker can be used to assistantly breed the Hostein cattle with the characteristic of heat stress resistance and higher milk yield.

The invention discloses a caproic acid fermented strain and a caproic acid extracting method. The caproic acid fermented strain is js-22-03, js-22-07 and js-22-12. Bred bacterial strains are good in passage stability, and the yield of the bred bacterial strains is increased to 1.90 g / 100 mL from 1.20 g / 100 mL compared with an original bacterial strain. The high-yield bacterial strains js-22-03, js-22-07 and js-22-12 are obtained through culture and screening, and the advantages of being short in fermentation time, stable in genetic property, high in yield and the like are achieved. The fermentation time is shortened to be within 10 days, and the caproic acid yield is increased to 1.90 g / 100 mL from original 1.20 g / 100 mL.

The invention relates to construction of saccharomyces cerevisiae engineering bacteria generating beta-phenethyl alcohol, a strain and application thereof, and belongs to the technical field of bioengineering and production of food spices. The prepared beta-phenethyl alcohol spice is a natural spice and can be used for preparing a plurality of food essences, and therefore, the beta-phenethyl alcohol can be widely applied to industries such as food, medicine and daily use chemicals. Engineering bacterial for coupling overexpression of an aminotransferase gene (ARO8) and decarboxylase gene (ARO10) in the anabolic way of beta-phenethyl are designed and constructed with saccharomycescerevisiae S288 as an original strain; gene copy and expression of the two key enzymes in the main synthetic way are enhanced; the bred engineering bacteria (transformants) are passaged a plurality of times and stable in inheritance; the metabolic flux and the yield of beta-phenethyl are obviously improved. With L-phenylalanine as a major raw material, the engineering bacteria are used for fermenting and converting the raw material into the beta-phenethyl alcohol spice which is single in configuration and chirality and high in fragrance quality, and therefore, a new idea and technical support are provided for preparation of the natural beta-phenethyl alcohol spice.

The invention discloses a desert oligotrophic bacterium DOB150 and application thereof to sand stabilization. Through isolation, screening, selective breeding and acclimatization in Gurbantunggut Desert of Junggar Basin of Xinjiang, the oligotrophic bacterium Bacillus sp. DOB150 CGMCC NO.11925 is obtained as a novel bacterium. The desert oligotrophic bacterium DOB150 and application thereof to sand stabilization have the advantages that after being sprayed into the desert, a bacterium agent prepared from the isolated oligotrophic bacterium DOB150 can play a role in sand stabilization, sand grain adhesion and quicksand activation control and provides good moisture conditions for germination of some plant seeds slow in germination to enable plants incapable of germinating due to insufficient moisture of soil to grow and develop, the number of major microorganisms in the soil can be increased effectively, major nutrient content of the soil can be increased, partial soil enzyme activity can be enhanced, algal crust can be recovered obviously, and accordingly the desert oligotrophic bacterium DOB150 is widely applicable.

The invention relates to a VII type Newcastle disease virus L-gene mutation attenuated vaccine strain produced by using a reverse genetic manipulation technology and a preparation method of the VII type Newcastle disease virus L-gene mutation attenuated vaccine strain, and belongs to the field of biological products and biological technologies. Toxicity of the VII type Newcastle disease virus L-gene mutation attenuated vaccine strain is weakened obviously, and at the same time, the vaccine strain on a chick embryo has high titer, stable heritability and high compatibility to an antigen of a prevalent virus, is suitable for large-scale production of vaccines, and can be used for producing the vaccines. Compared with conventional vaccines (gene I and gene II types), the VII type Newcastle disease virus L-gene mutation attenuated vaccine strain has a wide application prospect in control of incidence and prevalence of the Newcastle diseases.

The invention discloses a breeding method for local chicken variety resource protection and utilization, and belongs to the technical field of poultry breeding. According to the method, the local chicken variety resources are not manually selected, and the inherent characteristics of the original variety keep unchanged. During the utilization in each year, 12-week old cocks with the average body weight being 95 percent to 107 percent are selected and remained in a seed reserving group, and are subjected to hybrizing matching with cultured high-breeding-property or fast-growth new strains (matched strains), the appearance characteristics of the original local chickens are reserved, the egg laying performance / growth speed of the local variety is improved / accelerated by 15 to 20 percent. The method provided by the invention has the advantages that the original local variety is used as the strain for participating in the matching, the damage of current variety breeding or pure hybridization to resources in the past local chicken resource development and utilization is avoided, and the duplex effects of protection and utilization are achieved.

The invention discloses a method for improving the lateral branch rooting survival rate and the seed reproduction quality of radish. The method comprises the following steps: a second-stage lateral branch is taken as a cutting branch, after the cut-off lateral branch heals naturally, the cut-off lateral branch is soaked in an indolebutyric acid solution for 10 seconds and then inserted in a seedbed substrate composed of coco coir, vermiculite and mushroom dreg; a mixed solution containing Huabao No.1, Huabao No.2 and naphthylacetic acid is sprayed on the branch so that the branch can be acclimated and then survives; when new leaves come out, transplanting is performed by use of an underground simple culture tank filled with planting soil which is composed of coco coir, vermiculite, mushroom dreg and decomposed cow dung; after the cutting lateral branch survives, ventilation and light illumination are realized, Huabao No.1 and Huabao No.2 are sprayed, and after seeds are mature, the seeds are harvested. The method for improving the lateral branch rooting survival rate and the seed reproduction quality of the radish has the advantages that the method of rooting by use of the second-stage cutting lateral branch of the radish is adopted, a new method is provided for solving the problem of seed reproduction material loss of the radish due to the infringement of soft rot, the plants cultured by use of the method are well developed in root system, high in transplanting survival rate, and stable in heritability, and furthermore, the seed reproduction can be saved.

The invention aims at providing a porcine pseudorabies virus vaccine. The porcine pseudorabies virus vaccine comprises an antigen and a protective agent, wherein the antigen contains an attenuated virus strain which is prepared after deleting virulence genes by a porcine pseudorabies virus strain with the collection number of CGMCC No. 10266. The prepared vaccine can effectively prevent porcine pseudorabies; furthermore, because the porcine pseudorabies virus as the antigen is a gene-deleted strain, by continuous passage of horizontally transmitted infections in mouse bodies, no virulence reversion occurs, and the genetic stability is realized, thereby being in line with the standard of having no virulence reversion in the porcine pseudorabies virus deleted vaccine strain; and the prepared vaccine can provide effective immune protection, and has great commercialization development prospects.

A fast reproduction method of Anzu flower by inducing somatic cells includes such steps as picking up the young stem of Anzu flower, cutting by 3-7 mm in length, inoculating in the inducing culture meidum under aseptic condition, culturing at 25-27 deg.C in dark condition for 40-50 days to induce embryo of somatic cell, and culturing at 25-27 deg.C under 2000 LX (12-16 hr per day) for 50-60 days. Its advantage is fast speed.

The present invention discloses a breeding method for a white-feather intergenerically hybridized duck female parent. As an intergenerically hybridized feather color genetic mechanism is relatively complex, and selective advance by using a simple offspring phenotype is extremely slow, the invention creates a selecting method. By adopting a female parent offspring measuring method, by virtue of an artificial insemination technology and integrating an individual selection method and a family selection method, enclosed breeding and offspring production are performed for seed selection of about 5 generations, so that the white feather rate (the white feather area reaches over 95%) of intergenerically hybridized offsprings is significantly improved, and the genetic stability is good.

The invention relates to a method for producing a gene defect pseudorabies virus by culturing a passage cell in a tidal biological reactor. The method comprises the following steps of: (1) subcultring a recovery cell; (2) performing high-density cell culturing by using a specific biological reactor and a carrier system, attaching the cell on a vector, circulating a culture medium, filling oxygen and adjusting pH value and glucose; (3) performing virus infection by using the biological reactor, replacing the culture medium by using a virus culture medium, and inoculating the virus into the virus culture medium so as to ensure that the virus is fully adsorbed on the cell; (4) culturing and amplifying the virus under a specific condition; and (5) harvesting viruses so as to realize mass production.

The invention discloses a schizochytrium limacinum strain as well as a building method and application thereof. The strain disclosed by the invention is classified and named as schizochytrium sp. HX-RS, and the preservation number of the schizochytrium sp. HX-RS is CCTCC NO: M2017046. According to the strain, an acyltransferase functional domain originating from shewanella oneidensis PKS enzyme, instead of an acyltransferase functional domain originating from schizochytrium sp. PKS enzyme, is adopted; the strain is obtained through flat panel screening and acclimation screening with high rotation speed and low temperature. The strain can be used for co-producing DHA and EPA; the contents of the EPA and the DHA obtained by carrying out culture fermentation of the strain respectively reach 4.68% and 51.3%, and compared with an original strain, the contents of the EPA and the DHA are respectively increased by 69.9% and 14.3%; moreover, the fermentation production efficiency is high and the cost is relatively low.

The invention provides an escherichia coli recombination strain T002 for producing levodopa as well as a construction method and application thereof. According to the escherichia coli recombination strain, expression of 3-dehydrogenase shikimate dehydrogenase (aroE) is up regulated to enhance enzyme activity of 3-dehydrogenation shikimate dehydrogenase, and the levodopa can be further produced. The strain can produce the levodopa by utilizing glucose inorganic salt culture medium production, culture medium cost is reduced, and production cost of the levodopa is reduced. Furthermore, the recombination strain has the advantages of no plasmid and stable hereditary.

The present invention discloses a Tricholoma matsutake sing mutagenesis strain and a breeding method thereof. According to the present invention, a new high-yield Tricholoma matsutake sing strain is obtained through chemical mutagenesis screening separation, wherein the preservation number is CCTCC N0:2014671, the mycelium dry weight is increased by 69.68% compared with the original strain, the adenosine content is increased by 33.96%, the polysaccharide content is increased by 75.72%, the protein content is increased by 35.17%, and the mannitol content is increased by 52.8%; and after subculturing is performed on the new Tricholoma matsutake sing strain more than or equal to 10 times, no degeneration is generated, and the genetic stability is provided. The present invention further discloses an optimal fermentation culture medium of the Tricholoma matsutake sing mutagenesis strain.

The invention relates to a breeding method for improving the lean meat percentage of Meishan pigs and belongs to the field of animal genetic breeding. A Danish landraces as a male parent and a Meishan pigs as a female parent are hybridized in a three-way mode. Offspring breeding standards comprise the following standards: clothing hair is white, the total number born is larger than 14, the lean meat percentage is larger than 55%, and the daily gain is more than 630g. Suzhong pig generation II bred by the breeding method retains the characteristics of high total number born, good meat quality (intramuscular fat is larger than 3%), coarse feed resistance and easy feed of the Meishan pigs, the daily gain is increased to 634.74 grams from 439 grams and is increased by 44.59%, and the lean meat percentage is increased to 54.49% from 42.8% and is increased by 27.31%. The characteristics of fast growth, high lean meat percentage, and the like of the landraces are also retained, the total number born is increased to 14.24 from 11 and is improved by 29.5%, and the meat quality is also improved obviously.