354results about How to "Genetically stable" patented technology

The invention discloses a docosahexaenoic acid-producing bacterium and a mutation and screening method and application thereof. The strain is classified and named as Crypthecodinium cohnii HX-308, is preserved in China Center for Type Culture Collection, and has the preservation number M208246. The strain is a DHA-producing bacterium with high yield, has the DHA content 1.53 times of a starting strain, stable physiological-biochemical characteristic and stable heritability, and simultaneously can produce polyunsaturated fatty acid through liquid culture and fermentation; the content of unsaturated fatty acid reaches 20.4g / L, wherein the DHA content reaches 8.16g / L; and the strain is a good strain for producing natural DHA, and has excellent industrial production prospect.

The invention belongs to the field of pharmaceutic preparation, and relates to a targeted drug-carrying liposome of a tumor associated fibroblast. The surface of the targeted drug-carrying liposome is modified with a high-affinity ligand of a specific expression protein of the tumor associated fibroblast. The targeted drug-carrying liposome is capable of realizing targeted delivering of drugs into the tumor associated fibroblast, realizing intervention on tumor microenvironment via action on the tumor associated fibroblast, and realizing tumor treatment. And in addition, the tumor associated fibroblast targeted preparation can be combined with routine tumor cell targeted nano preparations so as to improve antitumous effects of the routine tumor cell targeted nano preparations.

First generation adenoviral vectors and associated recombinant adenovirus-based HIV vaccines which show enhanced stability and growth properties and greater cellular-mediated immunity are described within this specification. These adenoviral vectors are utilized to generate and produce through cell culture various adenoviral-based HIV-1 vaccines which contain HIV-1 gag, HIV-1 pol and / or HIV-1 nef polynucleotide pharmaceutical products, and biologically relevant modifications thereof. These adenovirus vaccines, when directly introduced into living vertebrate tissue, preferably a mammalian host such as a human or a non-human mammal of commercial or domestic veterinary importance, express the HIV1-Gag, Pol and / or Nef protein or biologically modification thereof, inducing a cellular immune response which specifically recognizes HIV-1. The exemplified polynucleotides of the present invention are synthetic DNA molecules encoding HIV-1 Gag, encoding codon optimized HIV-1 Pol, derivatives of optimized HIV-1 Pol (including constructs wherein protease, reverse transcriptase, RNAse H and integrase activity of HIV-1 Pol is inactivated), HIV-1 Nef and derivatives of optimized HIV-1 Nef, including nef mutants which effect wild type characteristics of Nef, such as myristylation and down regulation of host CD4. The adenoviral vaccines of the present invention, when administered alone or in a combined modality regime, will offer a prophylactic advantage to previously uninfected individuals and / or provide a therapeutic effect by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of HIV-1 infection.

The invention provides a trichoderma reesei strain capable of producing cellulase with high yield through space mutation, belonging to the technical field of microorganism mutation. According to the invention, trichoderma reesei Rw-X1 strain is preferably selected to be carried by Shenzhou IX to be subjected to space mutation and trichoderma reesei TG-C521 strain capable of producing cellulase with high yield can be carefully screened and cultured; the strain is collected in China General Microbiological Culture Collection Center on August 25, 2014 and has a collection Number of CGMCCNo.9537. According to the invention, the genetic character of the strain is changed by virtue of a special environment so that a strain with normal distributed mutation and stable genetic transformation can be screened out preferably; and the strain is used for producing cellulose and the filter paper activity of the strain is 25.83-34.49% higher than that of the trichoderma reesei Rw-X1 strain; the strain is friendly to the environment and is free of pollution, thus having a wide industrial application prospect.

The invention relates to an attenuated vaccine strain of a VII type new castle disease virus with a mutated L gene which is produced by reverse genetic operation technology and a preparation method thereof, and the invention belongs to the fields of biological products and biological technology. The virulence of the attenuated vaccine strain of the VII type new castle disease virus with the mutated L gene is obviously decreased, simultaneously the attenuated vaccine strain has the characteristics of high titer, stable heritability and high matching with the antigen of the epidemic virus according to the chicken embryo experiment; the preparation method is suitable for large scale production of vaccines, and is used for producing vaccines; compared with the conventional vaccines (gene I and gene II types), the attenuated vaccine strain of the VII type new castle disease virus with the mutated L gene has wide application prospects in the aspects of morbidity and epidemic control of new castle disease.

The invention provides newly separated enterococcus faecium (enterococcus faecium) HEW-A588. The enterococcus faecium HEW-A588 has obvious probiotic and stress resistance. When the micro-ecologic preparation prepared from the enterococcus faecium enters intestinal tracts of animals, the micro-ecologic preparation is rapidly activated and proliferated, the micro-ecologic preparation can have the efficacies of forming beneficial advantage microbial populations, maintaining the balance of the intestinal tracts, improving the food utilization efficiency of the animals, reducing the cost, improving the steady state of the internal environment of the digestive tracts in the animal bodies, promoting the absorption and the utilization of nutrition, promoting the growth of the animals, improving economic benefits and the like, and the micro-ecologic preparation has favorable application prospects.

The invention discloses a desert oligotrophic bacterium DOB150 and application thereof to sand stabilization. Through isolation, screening, selective breeding and acclimatization in Gurbantunggut Desert of Junggar Basin of Xinjiang, the oligotrophic bacterium Bacillus sp. DOB150 CGMCC NO.11925 is obtained as a novel bacterium. The desert oligotrophic bacterium DOB150 and application thereof to sand stabilization have the advantages that after being sprayed into the desert, a bacterium agent prepared from the isolated oligotrophic bacterium DOB150 can play a role in sand stabilization, sand grain adhesion and quicksand activation control and provides good moisture conditions for germination of some plant seeds slow in germination to enable plants incapable of germinating due to insufficient moisture of soil to grow and develop, the number of major microorganisms in the soil can be increased effectively, major nutrient content of the soil can be increased, partial soil enzyme activity can be enhanced, algal crust can be recovered obviously, and accordingly the desert oligotrophic bacterium DOB150 is widely applicable.

The invention discloses a method for improving the lateral branch rooting survival rate and the seed reproduction quality of radish. The method comprises the following steps: a second-stage lateral branch is taken as a cutting branch, after the cut-off lateral branch heals naturally, the cut-off lateral branch is soaked in an indolebutyric acid solution for 10 seconds and then inserted in a seedbed substrate composed of coco coir, vermiculite and mushroom dreg; a mixed solution containing Huabao No.1, Huabao No.2 and naphthylacetic acid is sprayed on the branch so that the branch can be acclimated and then survives; when new leaves come out, transplanting is performed by use of an underground simple culture tank filled with planting soil which is composed of coco coir, vermiculite, mushroom dreg and decomposed cow dung; after the cutting lateral branch survives, ventilation and light illumination are realized, Huabao No.1 and Huabao No.2 are sprayed, and after seeds are mature, the seeds are harvested. The method for improving the lateral branch rooting survival rate and the seed reproduction quality of the radish has the advantages that the method of rooting by use of the second-stage cutting lateral branch of the radish is adopted, a new method is provided for solving the problem of seed reproduction material loss of the radish due to the infringement of soft rot, the plants cultured by use of the method are well developed in root system, high in transplanting survival rate, and stable in heritability, and furthermore, the seed reproduction can be saved.

The invention relates to a method for producing a gene defect pseudorabies virus by culturing a passage cell in a tidal biological reactor. The method comprises the following steps of: (1) subcultring a recovery cell; (2) performing high-density cell culturing by using a specific biological reactor and a carrier system, attaching the cell on a vector, circulating a culture medium, filling oxygen and adjusting pH value and glucose; (3) performing virus infection by using the biological reactor, replacing the culture medium by using a virus culture medium, and inoculating the virus into the virus culture medium so as to ensure that the virus is fully adsorbed on the cell; (4) culturing and amplifying the virus under a specific condition; and (5) harvesting viruses so as to realize mass production.

The invention relates to a breeding method for improving the lean meat percentage of Meishan pigs and belongs to the field of animal genetic breeding. A Danish landraces as a male parent and a Meishan pigs as a female parent are hybridized in a three-way mode. Offspring breeding standards comprise the following standards: clothing hair is white, the total number born is larger than 14, the lean meat percentage is larger than 55%, and the daily gain is more than 630g. Suzhong pig generation II bred by the breeding method retains the characteristics of high total number born, good meat quality (intramuscular fat is larger than 3%), coarse feed resistance and easy feed of the Meishan pigs, the daily gain is increased to 634.74 grams from 439 grams and is increased by 44.59%, and the lean meat percentage is increased to 54.49% from 42.8% and is increased by 27.31%. The characteristics of fast growth, high lean meat percentage, and the like of the landraces are also retained, the total number born is increased to 14.24 from 11 and is improved by 29.5%, and the meat quality is also improved obviously.