118 results about "Shewanella oneidensis" patented technology

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses shewanella and application thereof in a microbiological fuel cell, and relates to the technical field of biology. The shewanella is Shewanella marisflavi EP1 which is preserved in 'China Center for Type Culture Collection' in January 2009 with the preservation number of CCTCC M 209016. The shewanella is characterized in that: the shewanella is gram-negative; the thalli are rod-shaped and straight; two ends are circular; the diameter is 0.3 to 1.0 micro; the length is 2.5 to 6.0 micros; the thalli can singly exist or are arranged in pair or in a short-chain shape; and the thalli have autogenetic flagella and can move. The shewanella is separated from offshore ocean sediments, has wide salt ion concentration tolerance activity, and can generate power in electrode liquid at the concentration of up to 8 percent. Therefore, the internal resistance of the microbiological fuel cell is effectively reduced.

The invention belongs to the field of biological nano-materials, and specifically relates to a preparation method of biological nano-ferrous sulfide. The preparation method comprises the specific steps: firstly, a phosphate-ferric sustained release system is constructed; secondly, shewanella oneidensis MR-1 is inoculated into an LB culture medium, subjected to activation culture, and then inoculated into the phosphate-ferric sustained release system under aseptic and anaerobic conditions, and when the OD600 value of the final concentration of the shewanella oneidensis MR-1 in the system is 0.1-2, inoculation with the shewanella oneidensis MR-1 stops; and then sodium thiosulfate mother liquor is added, and the biological nano ferrous sulfide is obtained by culturing, centrifuging and washing. The novel phosphate-ferric sustained release system is constructed to realize the quantitative control of ferric iron. A series of nano-ferrous sulfide with different catalytic properties can be controllably bio-synthesized by adjusting the adding amount of the sulfur source in synthesis, the catalytic performance is effectively regulated, and a new way is opened up for regulation of the catalytic performance of the bio-synthesized nano-material.

The invention discloses shewanella piezotolerans 34# and an application thereof to algae inhibition. The collection number of the shewanella piezotolerans 34# is CGMCC NO. 6490, and the shewanella piezotolerans 34# has the capacity of producing a long-chain QS (quorum sensing) signal and the function of inhibiting alga. A method provided by the invention has a good effect of inhibiting the red-tide algae, can be used for preventing red-tide organisms effectively, can realize the biological antagonistic prevention and control concept of controlling alga by bacteria and has field popularization value.

The present invention relates to a biological electrochemical detection method of fumaric acid, and belongs to the technical field of biochemical detection. The method is used for specific detection of the fumaric acid, a bacteria liquid of activate cultured shewanella spp. and a buffer are mixed to use as a reaction liquid of a biochemical electrochemical sensor, a fumaric acid-containing sample is added into the reaction liquid of the biochemical electrochemical sensor, a certain electric potential is controlled, change of output current signals is detected, and the fumaric acid concentration in the sample can be calculated according to the relationship between current response and the fumaric acid concentration change. The detection method does not rely on the large-scale instrument and equipment, reduces the cost of determination and operation difficulty, is free of interference of fumaric acid structural analogues, is high in specificity, high in accuracy, fast and efficient, accurate and reliable in test results, and can be applied to biological sample detection, food and drug detection, and cancer screening and diagnosis.

The invention discloses a microbial agent capable of efficiently biologically converting a form of cadmium in polluted soil. The microbial agent can be utilized for efficiently converting cadmium difficult to utilize in the polluted soil into soluble cadmium and mainly contains the following components in percentage by weight: 25%-35% of Candida utilis 15%-20% of Sporotrichum sp., 15%-20% of Acidithiobacillus ferrooxidans, 15%-20% of Sulfobacillus acidophilus and 5%-15% of Shewanella oneidensis. By processing the soil by virtue of the microbial agent, the removal rate of cadmium is 82%-95%. Compared with an existing cadmium polluted soil converting and restoring technique, the microbial agent is capable of efficiently converting the form of cadmium, short in cycle, low in cost and easy to generalize.

The invention provides an automatic biasing microbial coupled photoelectrocatalysis fuel cell pollution control system and an electrode preparation method, and belongs to the technical field of energyand pollution wastewater treatment. A C-ZnO/g-C<3>N<4>/BrOBi quaternary photocatalyst is prepared from low-cost materials through a two-step hydrothermal method. By taking the C-ZnO/g-C<3>N<4>/BrOBiquaternary photocatalyst as a negative electrode and by taking microbial shewanella oneidensis as a positive electrode, the automatic biasing microorganism coupled photoelectrocatalysis fuel cell pollution control system is established for degrading and processing dye and antibiotic wastewater in different light intensities. The invention achieves the beneficial effects that the quaternary negative electrode photocatalyst, by combining wastewater treatment and power generation and power utilization in a coupling system, can realize high-efficiency and low-energy-consumption water pollution control.

The invention belongs to the field of bioengineering, and relates to a shewanella putrefaciens phage isolated strain and application thereof. The shewanella putrefaciens phage isolated strain is shewanella putrefaciens phage SppYZU05 with the preservation number being CCTCC NO:M2016717. The invention also discloses bacteria inhibition application of the shewanella putrefaciens phage SppYZU05 as afood additive composition or a biological fresh-keeping agent in food production and storage, particularly, spoilage of fresh water or sea water products in cold chain circulation caused by shewanellaputrefaciens phage multiplication can be inhibited, and the product shelf life is prolonged.

The invention discloses an efficient electron carrier screening, designing and modifying method, and belongs to the field of bioelectrochemical catalysis. According to the invention, Shewanella oneidensis is used as an experimental object to screen and modify an electron carrier so as to improve the extracellular electron transfer capability of the electron carrier. In order to screen out electroncarriers with excellent performance, a single-chamber bioelectrochemical system and a double-chamber bioelectrochemical system are established, a microbial extracellular electron transfer detection method is established and perfected, and evaluation indexes such as cytotoxicity, current density, reduction equivalent and coulombic efficiency are determined. In order to further improve the performance of the electron carrier, the anthraquinone-2-sodium sulfonate (AQS) electron carrier is reasonably designed and modified based on a density functional theory, and seven substituent group positionsand six side chain groups are selected for theoretical calculation of standard Gibbs free energy change and oxidation-reduction potential. Experimental analysis proves that the method for reducing the redox potential of the electron carrier through design and transformation is an effective method for improving the extracellular electron transfer capacity of electroactive microorganisms.

The invention discloses P. denitrificans/S. oneidensis/graphene/calcium alginate denitrifying gel microsphere as well as preparation method and application thereof, and belongs to the technical fieldof environmental protection. According to the invention, calcium alginate is adopted to embed denitrifying microorganisms P. denitrifians, S. oneidensis MR-1 and graphene to form P. denitrifians/S. oneidensis MR-1/graphene/calcium alginate denitrifying gel microspheres, and the prepared microspheres have strong resistance to toxic and harmful environmental impact, and can realize recycling of strains and materials. When the microsphere is applied to an anaerobic denitrification system polluted by nitrate or co-polluted by nitrate, the time required by reaching 90% of nitrate removal rate can be greatly shortened, and the accumulation of intermediate products nitrite and nitrous oxide is reduced.

The invention discloses a method for rapidly removing hexavalent chromium pollutants by using an electron carrier, and belongs to the technical field of environment. The physiological property that the Shewanella oneidensis MR-1 can be used for removing hexavalent chromium pollutants is used as a basis; a strain is cultured to a stable growth period; thalli are collected and transferred to a reactor to carry out re-suspending; the hexavalent chromium pollutant removal capability of the electron carriers namely riboflavin and anthraquinone-2-sodium sulfonate is measured; under the condition that the concentration of riboflavin is 10-50 [mu]M, the Cr<6+> removal rate of riboflavin is increased to 1.40-2.26 times, the removal rate is increased to 42.0-67.7% from 30.0%, and the Cr<6+> removalrate is higher under the condition that the pH value is equal to 8; anthraquinone-2-sodium sulfonate can also improve the removal speed and removal rate of Cr<6+>, and under a concentration of 100 [mu]M, the maximum removal rate reaches 98.1% and is close to 100%. Hexavalent chromium pollutants can be quickly removed by utilizing the electron carrier, so that the problem of low removal efficiencyof hexavalent chromium pollutants at present is solved, and the method has potential environmental remediation and application values.

The invention discloses a method for repairing chromium-contaminated soil through a biochar-loaded hematite-shewanella loihica complex. Hematite is loaded on biochar by a hydrothermal method, then the hematite and shewanella loihica form a BC@ alpha-Fe2O3 @ loihiica complex, and fulvic acid is added as an electron shuttle, so that the chromium-contaminated soil is repaired. The ratio of the charcoal to the hematite is 10: 1; the proportion of the biochar loaded hematite in the complex is 0.05 g/mL (complex); and the repairing process is carried out under an anaerobic condition, the repairing time is 5 days, and the temperature is 30 +/-2 DEG C. The biochar-loaded hematite-shewanella loihica complex has a good remediation effect on soil polluted by hexavalent chromium with different concentrations, the remediation time is short, the biochar-loaded hematite-shewanella loihica complex can be used for remediation of soil polluted by high-concentration hexavalent chromium, and an efficient and rapid method is provided for clay polluted by high-concentration hexavalent chromium which is difficult to remedy.

The invention discloses a method for degrading sulfamethoxazole. According to the method, sulfamethoxazole is degraded through the synergistic effect of P.denitrificans and shewanella. Accumulation ofintermediate products is reduced under the interaction of microbial flora, and finally anaerobic denitrification is achieved.

The invention relates to the field of pollution management and discloses a method for digesting bottom mud of black and odorous river channel through a microorganism preparation. The microorganism preparation is added into a black and odorous river channel; the microorganism preparation is prepared by mixing CQ1, CQ2 and CQ3 according to the volume ratio of (1 to 3) to (1 to 3) to 1; the CQ1 is amixed bacterium agent of bacillus subtilis, bacillus aureus, acinetobacter, psychrobacter, acinetobacter and pseudomonas strains; the CQ2 is a mixed bacterium agent of arcobacter, acinetobacter and shewanella oneidensis strains; and the CQ3 is a mixed bacterium agent of aeromonas, ralstonia eutropha and ralstonia eutropha strains. By adopting the method disclosed by the invention, the problems that original biological population structures and living conditions of rivers and lakes are damaged in a traditional dredging manner, a self-cleaning function is weakened so that negative influences onecological restoration are caused and secondary pollution is easy to cause, principal and secondary aspects cannot be treated at the same time, people are likely to be disturbed and the management price is high are solved.