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Biological electrochemical detection method of fumaric acid

A bioelectrochemical and detection method technology, applied in the field of bioelectrochemical detection of fumaric acid, can solve the problems of high detection cost, long response time, and complicated operation process, and achieve high consistency, specificity, and high The effect of sensitivity

Active Publication Date: 2014-11-26
JIANGSU UNIV
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Problems solved by technology

[0010] The purpose of the present invention is to provide a new method for specific determination of fumaric acid, to solve the problems of high detection cost, expensive detection equipment, long response time, complicated operation process and poor repeatability in the prior art

Method used

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  • Biological electrochemical detection method of fumaric acid
  • Biological electrochemical detection method of fumaric acid
  • Biological electrochemical detection method of fumaric acid

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Bacteria used: Shewanella ( Shewanella oneidensis ), purchased from ATCC American Type Culture Collection, strain number ATCC700550.

[0038] (2) Obtaining the working bacteria solution: Inject 200 μL of glycerol bacteria into 45 mL of LB medium (containing tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 5 g / L, pH=7.0) (Shewanella was mixed with 30% glycerol aqueous solution in equal proportions and stored in a -70°C refrigerator), and cultured at a temperature of 30°C and a shaking speed of 200 rpm for 16 h to obtain a bacterial liquid.

[0039] (3) Preparation of reaction solution for bioelectrochemical sensors: 95 mL of M9 medium (containing Na 2 HPO 4 .12H 2 O 17.8 g / L, KH 2 PO 4 3g / L, NaCl 0.5g / L, NH 4 Cl 1 g / L) and 5 mL LB liquid medium (containing tryptone 10 g / L, yeast extract 5 g / L and sodium chloride 5 g / L, pH=7.0) were mixed thoroughly, and sodium lactate, chloride Calcium, magnesium sulfate to the final concentration of 18 mM, 0.1 mM and...

Embodiment 2

[0049] It is basically the same as Example 1, but with the following changes: the working electrode is carbon felt. The test chart obtained is attached Figure 6 shown.

Embodiment 3

[0051] It is basically the same as Example 1, but with the following changes: the applied voltage of the working electrode is -0.4 volts. Current signal such as Figure 7 shown.

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Abstract

The present invention relates to a biological electrochemical detection method of fumaric acid, and belongs to the technical field of biochemical detection. The method is used for specific detection of the fumaric acid, a bacteria liquid of activate cultured shewanella spp. and a buffer are mixed to use as a reaction liquid of a biochemical electrochemical sensor, a fumaric acid-containing sample is added into the reaction liquid of the biochemical electrochemical sensor, a certain electric potential is controlled, change of output current signals is detected, and the fumaric acid concentration in the sample can be calculated according to the relationship between current response and the fumaric acid concentration change. The detection method does not rely on the large-scale instrument and equipment, reduces the cost of determination and operation difficulty, is free of interference of fumaric acid structural analogues, is high in specificity, high in accuracy, fast and efficient, accurate and reliable in test results, and can be applied to biological sample detection, food and drug detection, and cancer screening and diagnosis.

Description

technical field [0001] The invention relates to a bioelectrochemical detection method for fumaric acid, belonging to the technical field of biochemical detection. Background technique [0002] Fumaric acid, also known as fumaric acid, or fumaric acid (IUPAC name (E)-butenedioic acid), is a colorless, flammable crystal, a carboxylic acid derived from butene. Its chemical formula is C 4 h 4 o 4 . Fumaric acid is an important four-carbon platform organic chemical raw material and fine chemical product. Fumaric acid and its derivatives are widely used in food, medicine, feed, chemical industry, paint, resin due to their non-toxic and low corrosion characteristics. , plasticizers and other fields. The rapid and accurate determination of its content is of great significance in the synthesis of fumaric acid and the production of its derivatives. According to reports (W. Marston Linehan & Tracey A. Rouault. Molecular Pathways: Fumarate Hydratase-Deficient Kidney Cancer: Target...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 雍阳春高璐司荣炜翟丹丹孙建中
Owner JIANGSU UNIV
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