818 results about "Enzyme system" patented technology

The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide-capped RNA also provides methods and kits for obtaining only type-specific or condition-specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

The invention relates to modification of a cell penetrating peptide for realizing a low-toxicity administration system with a positive targeting selecting function. A shielding peptide, an enzymolysis substrate peptide and a cell-penetrating peptide are connected in sequence, so that an activatable cell penetrating peptide is formed; and a medicament and/or a tracer and/or a medicament carrier is connected or embedded or adsorbed to the cell penetrating peptide, so that an administration system is constructed. According to a shielding peptide sequence, positive charges carried on the surface of the administration system can be reduced or completely neutralized, the cell penetrating capability of the cell penetrating peptide is shielded, and the toxicity of the administration system on normal cells of an organism is lowered; and an enzymolysis substrate peptide sequence can be identified by enzyme systems secreted specifically by different pathological change tissue cells and fractured by enzyme hydrolysis, so that a cell penetrating peptide is released and is used for carrying a medicament and/or a medicament carrier through a cell membrane, and the medicament enters cells and is brought into play. The invention aims to actively convey an antitumor medicament to tumor tissues in a targeted way and make the antitumor medicament enter tumor cells to a larger extent by using the administration system which can be used for activating a cell penetrating function, so that the toxicity at a non-tumor position is lowered while the antitumor effect of the medicament is enhanced.

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

The invention provides a real-time fluorescence quantitative PCR (Polymerase Chain Reaction) kit for detecting the expression level of HER2 genes and relates to a PCR kit. The real-time fluorescence quantitative PCR kit comprises PCR reaction liquid of the HER2 genes, PCR reaction liquid of ACTB genes, a Taq enzyme system, a control product and a packaging object; and the adopted primer is an annular primer, 3-8 self-designed basic groups are at the 5' end, and can be combined with the sequence at the 3' end under proper conditions so as to form double chains. The real-time fluorescence quantitative PCR kit has the advantages that the non-specific amplification products especially primer dimer generated by nonspecific amplification products especially the primer can be effectively prevented from being formed, the specificity is increased; and a relative-quantitative RT-PCR method is utilized for detecting the expression level of the HER2 genes of a patient with the breast cancer, adopts a 2-delta deltaCt value method for quantifying the detected result, and can be used for diagnosis of early stage and transferring of the breast cancer and assisting multiple fields such as clinicalmedicine selection and prognosis.

The invention relates to a method for preparing a silver-loaded modified bacterial cellulose-based composite functional wet dressing, which comprises the following steps: (1) placing the freeze-dried bacterial cellulose in a nitroxyl/enzyme system solution, adding an oxidizing agent reaction; (2) adding diamine substances to the solution, heating and reacting; (3) then soaking in silver nitrate solution to obtain bacterial cellulose containing selectively loaded silver ions; Obtain bacterial cellulose selectively loaded with nano-silver particles; (4) finally add the compound solution, take it out after standing at room temperature, and after partial drying, packaging, and sterilization, the silver-loaded modified bacterial cellulose-based compound type can be obtained Functional wet dressing. In the method of the invention, the antibacterial nano-silver can be loaded on the C6 hydroxyl group of the bacterial cellulose, and the functional medicine can be compounded. The prepared dressing can treat various skin injuries, wounds, burns and scalds, etc., and has good antibacterial and repairing effects.

The invention relates to a compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate and a preparation method thereof. The use amount of calcium nitrate is reduced, and the time for treating the black and odorous rivers is shortened. The compound microbial preparation is prepared from 40-80 parts of thiobacillus denitrificans and thiocapsa roseoppersicina mixed powder, 10-60 parts of composite bacillus subtilis powder and 10-60 parts of yeast and lactic acid bacterium mixed powder. By means of the preparation, sulfide in black and odorous bottom sludge, organic matter and strains suitable for growing in the black and odorous bottom sludge are screened and degraded in a targeted mode; by means of thiobacillus denitrificans with efficient hydrogen sulfide degrading and denitrification capacity, sulfur-oxidizing bacteria of thiocapsa roseoppersicina with efficient hydrogen sulfide and ammoniacal nitrogen degrading capacity, bacilli rich in protease, amylase, cellulose and other enzyme systems, yeast and lactic acid bacteria, a small amount of calcium nitrate and the compound microbial preparation are thrown into the black and odorous rivers so that water quality of the black and odorous rivers can be quickly improved, the black and odorous bottom sludge can be removed quickly, and the thickness and the organic matter content of the bottom sludge can be quickly reduced.

The invention relates to a feed additive, in particular to a prepration method of a compound microbial feed additive, which is used for solving the problems that a variety of feed additives have respective different shortcomings in the actual applications. The preparation method comprises the following steps: preparing selenium yeast, iron yeast and zinc yeast which are rich in trace elements, taking vinegar residue, wheat bran, corn flour, compound Daqu, ammonium sulfate and other auxiliary materials as culture media, adding aspergillus niger, aspergillus oryzae, bacillus subtilis, lactobacillus and yeast, producing a multi-bacterial compound enzyme by the solid-state method, and mixing the functional yeast, phytase and the multi-bacterial compound enzyme for producing a biological feed micro-multi-enzyme which has a large number of live bacteria in beneficial bacterial groups, a complete enzyme system of digestive enzymes and non-digestive enzymes needed by animals and high enzyme activity, and is rich in organic trace elements, vitamins, amino acids and unknown growth-promoting factors with functional nutrition for the animals and people; furthermore, the feed additive has the advantages of advanced process, abundant nutrition, abundant bacterial groups, full enzyme spectrum, high activity, greenness, no pollution and the like.

The invention discloses a gracilaria lemaneiformis agar oligosaccharide and the preparation method and application thereof and relates to an oligosaccharide. The invention provides a gracilaria lemaneiformis agar oligosaccharide, the preparation method of gracilaria lemaneiformis agar oligosaccharide and the application of the gracilaria lemaneiformis agar oligosaccharide in preparation of antioxidant and uvioresistant health care products and cosmetics. Pacific heating color bacillus H2 and gracilaria lemaneiformis are co-cultivated and the gracilaria lemaneiformis is directly degraded by the enzyme system which is generated by bacterial strain to obtain the gracilaria lemaneiformis agar oligosaccharide of which the degree of polymerization is 4-8. The gracilaria lemaneiformis agar oligosaccharide has the physiological activities of antioxidation, absorbing ultraviolet rays and the like. In preparation, the bacterial strain is activated by an activation medium plate and inoculated in a seed medium; fermentation broth is obtained by shake cultivation and inoculated in a saccharide generating medium; after shaking fermental cultivation and centrifugalization, supernate is collected; the supernate is successively filtered by a membrane filtration devices with a 0.45mu m filter membrane, a 0.22mu m filter membrane, a 10000-50000D ultrafiltration membrane and a 300-600D ultrafiltration membrane, and the percolate is the gracilaria lemaneiformis agar oligosaccharide.

The invention belongs to the field of food processing and particularly relates to a method for adding medical stone for fermentation in production of white liquor, which comprises the steps of preparing raw materials, steaming grains, fermenting and discharging from kiln and is characterized in that the medical stone is added in the fermentation process of white liquor. The method has the advantages that: by adding the medical stone in the fermentation process of the white liquor, the growth of a zymophyte strain is promoted, the combination structure of an enzyme system in a fermentation process is optimized, fermentation substrate is well transformed, the materials can be well combined, the quantity and types of the products are increased, the complex property of the quality of the liquor is increased, and the quality and yield of the liquor are increased. According to sensory evaluation, the produced raw wine is clear and transparent, has strong grain fragrance, and offers a mellow, soft, sweet and smooth mouthfeel.

The invention provides a preparation method for a degummed kenaf fiber. According to the method, vacuum technology, an enzyme system and ultrasonic technology are combined together for degumming of kenaf bast fibers. The method comprises the following steps: carrying out degumming on a kenaf fiber by using a mixed enzyme system, allowing enzyme liquid to fully penetrate into the fiber by using vacuum negative pressure technology and allowing gum macro-molecule insoluble in water to fracture and a part of lignin to be decomposed at the same time; and removing gum strongly bonding with the fiber by using low-frequency ultrasonic waves and finally, removing residual gum in tiny parts like micropores and slits by using high-frequency ultrasonic waves. The method overcomes the problems of a great amount of residual gum, rough fiber surface and the like in conventional degumming with a single biological enzyme, improves the quality of the degummed kenaf fiber and reduces the usage amount of the enzyme and chemicals.

The invention relates to a method for preparing a germinated brown rice ferment drink. Germinated brown rice is processed in an Aspergillus oryzae and Rhizopus oryzae mixed culture manner, is decomposed by using the enzyme system complementarity, and is inoculated with lactic acid bacteria to carry out fermentation. The prepared germinated brown rice ferment drink is rich in organic acids, variousfree amino acids, soluble dietary fibers, and active components which are gamma-aminobutyric acid and SOD, and has the advantages of sour-sweet mouthfeel, convenience in drinking, no additives, excellent nutrition and healthcare effects, great improvement of the raw material utilization rate in a two-stage fermentation process, and great solving of resource wastes of existing brown rice having bad mouthfeel.