148 results about "Cellulose binding" patented technology

This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and / or depolymerize cellulose. These systems have a number of applications. Some embodiments relate to a method of producing ethanol using the cell wall degradative systems of the present invention.

The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and / or depolymerize cellulose. These systems have a number of applications.

Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.cip1 cDNA sequence (SEQ ID NO: 1)GACTAGTTCA TAATACAGTA GTTGAGTTCA TAGCAACTTC50TGAACAAATT ATCTGCGCAA ACATGGTTCG CCGGACTGCT100CTGCTGGCCCTTGGGGCTCT CTCAACGCTC TCTATGGCCC AAATCTCAGA150CGACTTCGAGTCGGGCTGGG ATCAGACTAA ATGGCCCATT TCGGCACCAG200ACTGTAACCAGGGCGGCACC GTCAGCCTCG ACACCACAGT AGCCCACAGC250GGCAGCAACTCCATGAAGGT CGTTGGTGGC CCCAATGGCT ACTGTGGACA300CATCTTCTTCGGCACTACCC AGGTGCCAAC TGGGGATGTA TATGTCAGAG350CTTGGATTCGGCTTCAGACT GCTCTCGGCA GCAACCACGT CACATTCATC400ATCATGCCAGACACCGCTCA GGGAGGGAAG CACCTCCGAA TTGGTGGCCA450AAGCCAAGTTCTCGACTACA ACCGCGAGTC CGACGATGCC ACTCTTCCGG500ACCTGTCTCCCAACGGCATT GCCTCCACCG TCACTCTGCC TACCGGCGCG550TTCCAGTGCTTCGAGTACCA CCTGGGCACT GACGGAACCA TCGAGACGTG600GCTCAACGGCAGCCTCATCC CGGGCATGAC CGTGGGCCCT GGCGTCGACA650ATCCAAACGACGCTGGCTGG ACGAGGGCCA GCTATATTCC GGAGATCACC700GGTGTCAACTTTGGCTGGGA GGCCTACAGC GGAGACGTCA ACACCGTCTG750GTTCGACGACATCTCGATTG CGTCGACCCG CGTGGGATGC GGCCCCGGCA800GCCCCGGCGGTCCTGGAAGC TCGACGACTG GGCGTAGCAG CACCTCGGGC850CCGACGAGCACTTCGAGGCC AAGCACCACC ATTCCGCCAC CGACTTCCAG900GACAACGACCGCCACGGGTC CGACTCAGAC ACACTATGGC CAGTGCGGAG1000GGATTGGTTACAGCGGGCCT ACGGTCTGCG CGAGCGGCAC GACCTGCCAG1050GTCCTGAACCCATACTACTC CCAGTGCTTA TAAGGGGATG AGCATGGAGT1100GAAGTGGAGA GAGTTGAAGT GGCATTGCGC TCGGCTGGGT1150CAGCAGCTAT GAATACTCTA TGTGATGCTC ATTGGCGTGT1200AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA1250AAAAAAAAAG GGGGCGGCCG C1271

A method of delivering a benefit agent to a selected area of a fabric for exerting a predetermined activity, wherein the area is pre-treated with a multi-specific binding molecule which has a high binding affinity to said area through one specificity and is capable of binding to said benefit agent through another specificity, followed by contacting said pre-treated area with said benefit agent, to enhance said pre-determined activity to said area. Preferably, the binding molecule is an antibody or fragment thereof, or a fusion protein comprising a cellulose binding domain and a domain having a high binding affinity to another ligand which is directed to said benefit agent. The method is useful for stain removal, perfume delivery, and treating collars and cuffs for wear.

Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.

A method of delivering a benefit agent to fabric for exerting a pre-determined activity, wherein the fabric is pre-treated with a multi-specific binding molecule which has a high binding affinity to the fabric through one specificity and is capable of binding to the benefit agent through another specificity, followed by contacting the pre-treated fabric with the benefit agent, to enhance the pre-determined activity to the fabric. Preferably, the binding molecule is an antibody or fragment thereof, or a fusion protein comprising a cellulose binding domain and a domain having a high binding affinity to another ligand which is directed to said benefit agent. The method is useful for example for stain removal, perfume delivery, and treating collars and cuffs for wear.

The present invention relates to a polynucleotide encoding a recombinant scaffolding polypeptide comprising at least a signal peptide, a Cellulose Binding Domain, two cohesin domains and an S-layer Homology domain, wherein said isolated polynucleotide preferably comprises all or an active part of the nucleotide sequence as set forth in SEQ ID NO:2. The present invention further relates to vectors comprising such polynucleotides, recombinant lactic acid bacteria, and method for degrading a cellulosic biomass using such recombinant lactic acid bacteria.

The invention belongs to the field of recombinant protein expression and purification. The invention provides a method for expressing and purifying recombinant protein in a eukaryote by using a family 3 cellulose binding domain CBM3 serving as an affinity tag for recombinant protein expression. The method comprises the following steps of: (1) constructing a nucleotide sequence of coded CBM3 optimized by a eukaryote codon; (2) constructing a recombinant construct of the nucleotide sequence of the coded CBM3 optimized by the eukaryote codon in the step (1) and a nucleotide sequence of coded target protein; (3) cloning the recombinant construct constructed in the step (2) to an expression vector suitable to be expressed in the eukaryote; (4) expressing the recombinant expression vector obtained in the step (3) in the eukaryote; and (5) purifying the expressed recombinant protein by using the CBM3 as the affinity tag and using cellulose. The invention also provides a method for expressingand purifying the recombinant protein in the eukaryote by using the CBM3 serving as the affinity tag for the recombinant protein expression and internal protein, wherein the method comprises a step of constructing a target protein-internal protein-CBM3 recombinant construct so as to cut out and purify the recombinant protein of fusion expression without additional amino acid.

Cellulase fusion proteins comprising an endoglucanase core region and a heterologous cellulose binding domain are described. The fusion proteins may be produced by recombinant techniques using appropriate polynucleotides, expressing vectors and host cells. The fusion proteins and enzyme preparations thereof are useful in treating cellulosic material, such as textile material, and they are particularly useful in biostoning denim or in biofinishing fabrics and garments. In addition the fusion proteins may be used in pulp and paper industry, oil extraction from plants, detergent compositions, or for improving the quality of animal feed.

The invention relates to drylaid nonwoven materials comprising bicomponent fibres comprising a low melting polyolefin component and a high melting polyolefin component, the low melting polyolefin component constituting at least a part of the surface of the fibre and comprising a non-grafted polyolefin component and a grafted polyolefin component, wherein the grafted polyolefin component has been grafted with an unsaturated dicarboxylic acid or an anhydride thereof, e.g. with maleic acid or maleic anhydride. The bicomponent fibres fibres have an excellent bonding affinity for natural fibres such as cellulose pulp fibres and allow the production of airlaid nonwovens with reduced generation of dust during the production process and with improved nonwoven strength properties.

Use of a nicotine-cellulose combination and a gum base for the preparation of a chewing gum composition for achieving a fast onset of nicotine effect after initiation of chewing the chewing gum composition by a subject. The chewing gum composition is preferably prepared by direct compression and it does not disintegrate during chewing. The invention also relates to chewing gum compositions comprising nicotine, which compositions provide a rapid release of nicotine.

The present invention relates to medicine biological engineering technology, and is cDNA of small peptide encoding human parathyroid hormone and the method of preparing human parathyroid hormone with its expression vector. Through PCR amplification of chemically synthesized recombinant human human parathyroid hormone (1-34) gene and cloning to expression vector pET-35b(+), recombinant human human parathyroid hormone (1-34) is fused to the carboxyl end of cellulose combining structure domain (CBDclose) and efficient expressed. The fusion protein is treated through cellulose resin affinity chromatographic purification, Factor Xa schizolysis to release rhPTH(1-34), the second cellulose resin affinity chromatography, and C4 reverse efficient liquid chromatographic purification to obtain pure rhPTH(1-34). The sample is tested to have molecular weight of 4117.0 Da.

The invention discloses titanium dioxide for decorative paper and a preparation method. The titanium dioxide comprises a titanium dioxide base material and a coating layer located on the surface of the titanium dioxide base material, and the coating layer sequentially comprises a titanium phosphate and aluminum phosphate mixed coating layer and a magnesium oxide and aluminum oxide mixed coating layer from the inside out. According to the titanium dioxide for decorative paper provided by the invention, a titanium phosphate and aluminum phosphate mixed film layer is coated on the surface of titanium dioxide, the hue of the titanium dioxide can be improved, the coating process of the titanium dioxide can be reduced, and the cost is reduced, and then an aluminum oxide and magnesium oxide mixedfilm layer is coated to improve the isoelectric point of the titanium dioxide, so that the titanium dioxide is positively charged in a papermaking system and can be better combined with negatively charged cellulose, the retention rate of the titanium dioxide in paper is improved, and then the covering power of the titanium dioxide is further improved. The invention further provides a preparationmethod of the titanium dioxide by taking the technical scheme as a starting point.