391 results about "Binding ability" patented technology

An oligo- or polynucleotide analogue having one or more structures of the general formulawhere B is a pyrimidine or purine nucleic acid base, or an analogue thereof,is disclosed. The use of this analogue provides an oligonucleotide analogue antisense molecule, which is minimally hydrolyzable with an enzyme in vivo, has a high sense strand binding ability, and is easily synthesized.

This invention relates to a method for producing a zinc finger nucleic acid binding protein comprising preparing a zinc finger protein according design rules, varying the protein at one or more positions, and selecting variants which bind to a target nucleic acid sequence by polysome display.

The present invention is directed to methods for purifying a non-human antibody, or antigen binding portion thereof, exhibiting weak binding strength and low binding capacity for Protein A chromatography media. In one aspect, a kosmotropic salt solution is employed to promote the hydrophobic interaction between the non-human antibody, or antigen binding portion thereof, and the Protein A ligand, thereby enhancing the binding of the non-human antibody, or antigen binding portion thereof, to the Protein A chromatography media. In another aspect, the concentration of the non-human antibody, or antigen binding portion thereof, in a sample comprising the antibody, or antigen binding portion thereof, exposed to a Protein A chromatography media is increased to enhance the binding of the non-human antibody, or antigen binding portion thereof, on the Protein A chromatography media.

The invention relates to the immune analysis field, in particular relating to a method for performing an immunological test on biomolecules by avidin / streptavidin magnetic composite particles, which takes avidin / streptavidin magnetic composite particles as carriers, and comprises the following steps:, coating biotinylated antibody or antigen, adding a specimen to be tested and enzyme labeled antibody / enzyme labeled antigen / enzyme labeled anti-antibody, adding chromogenic substrate and testing.Biotin and avidin / streptavidin has strong binding capacity, can fix the antigen / antibody labeled by the biotin through the avidin / streptavidin labeled on the surface of the magnetic composite particles so as to use the system to test specific antibody / antigen. The test system has the advantages of high sensitivity, high specificity, high stability, strong adaptability and the like.

The present invention provides efficient methods based on alteration of the protein A-binding ability, for producing or purifying multispecific antibodies having the activity of binding to two or more types of antigens to high purity through a protein A-based purification step alone. The methods of the present invention for producing or purifying multispecific antibodies which feature altering amino acid residues of antibody heavy chain constant region and / or variable region. Multispecific antibodies with an altered protein A-binding ability, which exhibit plasma retention comparable or longer than that of human IgG1, can be efficiently prepared in high purity by introducing amino acid alterations of the present invention into antibodies.

Methods for improving the specific binding ability of bispecific binding compositions are described. The bispecific binding compositions are able to target cells by a high affinity targeting domain to a target cell surface marker and a low affinity binding domain that binds specifically to a second cell surface marker, wherein the binding of each domain to its respective cell surface marker increases or decreases, as desired, the biological activity of the respective cell surface markers. The invention further provides bispecific binding agents for use in the methods, as well as uses for the agents.

The present invention relates to the identification and cloning of a novel neutralizing human monoclonal antibody to the Respiratory Syncytial Virus. The invention provides such antibodies, fragments of such antibodies retaining RSV-binding ability, chimeric antibodies retaining RSV-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Finally, the invention provides for diagnostic and therapeutic methods employing the antibodies and nucleic acids of the invention.

The invention relates to the field of medicaments, in particular to an integrin blocking agent HM-3 which has the function of inhibiting tumor angiogenesis, integrin affinity and a bonding capacity and application thereof. The blocking agent is a polypeptide modified with polyethylene glycol, and the modified integrin blocking agent polypeptide can be applied to treatment of solid tumors. During application of the integrin blocking agent to preparation of a tumor treatment medicament, the sequence and structure of the integrin blocking agent is mPEG-SC20k-Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro-Gly-Gly-Gly-Gly-Arg-Gly-Asp. The integrin blocking agent polypeptide designed in the invention is scientific, reasonable, practical and effective, can be used for preparing a treatment medicament for treating human solid tumors, and has remarkable social value and market value; and the treatment spectrum of the integrin blocking agent is expanded greatly, and novel thought and prospect are provided for future development of medicaments.

The present invention describes devices and methods for performing protein analysis on laser capture microdissected cells, which permits proteomic analysis on cells of different populations. Particular disclosed examples are analysis of normal versus malignant cells, or a comparison of differential protein expression in cells that are progressing from normal to malignant. The protein content of the microdissected cells may be analyzed using techniques such as immunoassays, 1D and 2D gel electrophoresis characterization, Western blotting, liquid chromatography quadrapole ion trap electrospray (LCQ-MS), Matrix Assisted Laser Desorption Ionization / Time of Flight (MALDI / TOF), and Surface Enhanced Laser Desorption Ionization Spectroscopy (SELDI). In addition to permitting direct comparison of qualitative and quantitative protein content of tumor cells and normal cells from the same tissue sample, the methods also allow for investigation of protein characteristics of tumor cells, such as binding ability and amino acid sequence, and differential expression of proteins in particular cell populations in response to drug treatment. The present methods also provide, through the use of protein fingerprinting, a rapid and reliable way to identify the source tissue of a tumor metastasis.

The present invention discloses a high-performance flame-proof abrasion-resisting composite material which is prepared mainly by the components of particle material alumina or / and corundum or / and mullite 50-70 parts; hard particle 5-25 parts; binding agent 8-25 parts; aluminum oxide micro powder or / and silicon micronized quartz powder 8-15 parts and surfactant 0.1 parts. The composite material is developed according to the scientific knowledge obtained in the long-term researching and producing for the flame-proof abrasion-resisting composite material in our company and absorbing the latest accomplishment of the present material science, the material has the advantages of increasing the high-temperature bonding strength and toughness of the composite material, increasing the binding ability of the interface between the composite material and the equipment, facilitating the two sides forming a compact and high-strength whole body, optimizing the capability of the composite material in the high-temperature state, prolonging the service lifetime of the equipment, reducing the number of the breakdown maintenance, reducing the material consumption and the labor intensity of the worker thereby obtaining the purpose of increasing the economic benefit of the enterprise.

The present disclosure relates generally to multi-specific Fab fusion proteins (MSFP) which comprise an antibody Fab fragment with both N-termini fused to a fusion moiety (fusion moiety A or B). MSFP containing the Fab fragment exhibit significantly reduced binding ability of the Fab fragment to the Fab target. Binding of the Fab to its target is restored when the MSFP is clustered on a cell surface by binding of the fusion moieties to their target. The reduced binding of the Fab to its target, especially when presented on a cell surface in its native state, absent fusion moiety binding provides advantages such as: reduced side effects and allows desirable pharmacological effects of selectivity and specificity in a controlled manner.

The invention relates to an ADH (ethanol dehydrogenase) protein family mutant and an application thereof, and particularly provides a mutant of an ADH protein. Compared with wild type ADH proteins, the ADH protein mutant is capable of remarkably increasing expression purity, efficiency and yield of foreign proteins in a cell-free synthesis system in vitro, and / or reducing binding capacity of the mutant protein and the Ni medium.

The embodiment of the invention discloses an infrared high-emissivity coating material which is prepared by mixing an infrared high-emissivity powder material and a colloid material, wherein the infrared high-emissivity powder material is prepared from iron-ore slag, iron oxide, manganese oxide, zirconium oxide, chromic oxide and silicon carbide through a treatment process of ball milling, mixing, high-temperature roasting and superfining; the colloid material is prepared by mixing water, sodium silicate, carboxymethyl cellulose and superfine silicon dioxide according to a certain weight ratio. The coating material has the advantages of high infrared emitting ability, stability, difficulty in aging, high binding ability of the coating with a substrate, long service life, low price and the like.

The invention discloses a triiodothyronine quantitative detection kit which comprises magnetic particle suspension coated with diiodothyronine-gelatin, a triiodothyronine series calibrator, a triiodothyronine antibody labelled by horseradish peroxidase, a dissociation agent, a chemiluminescent substrate A, a chemiluminescent substrate B and wash concentrate. The invention further discloses a preparation method of the kit. The invention has the advantage that a chemical structural analogue of hormone is coated by a method for coating an antigen analogue with a labelled antibody, and the analogue and the detected hormone have identical immune correlation, thus realizing specific binding of the analogue and the antibody of the hormone; but the binding capability of the analogue with thyroid-binding protein (THBP) is significantly lowered, thus reducing influence of the binding protein on a measuring system to a large extent. The kit has greatly improved sensitivity and precision, greatlyenlarged detection range, greatly shortened reaction time, enhanced product properties and lowered product cost.

Provided are an electrode for solid-state batteries, a method of preparing the electrode, a solid-state battery including the electrode, and a bonding film used for the method of preparing the electrode. The electrode for solid-state batteries include a bonding layer interposed between an electrode layer and a current collecting member and bound to the electrode layer, where the bonding layer includes a first binder which is inactive to the solid electrolyte, a second binder which has a stronger binding ability to the current collecting member than a bonding strength of the first binder to the current collecting member; and a bonding layer conductive material.

The invention provides a bispecific antibody. The bispecific antibody is composed of a single brand unit and a univalent unit, wherein the single brand unit has specific binding ability on surface antigen CD3 of immune cells, and the univalent unit has specific binding ability on surface antigen EGFR of tumor cells; and the single brand unit includes a single brand variable fragment (ScFv) fused with an Fc fragment, and the univalent unit includes a light strand and heavy strand pair. The invention also provides a preparation method and a pharmaceutical use of the bispecific antibody.

The invention relates to an antibody or an antigen-binding fragment thereof that binds to a CD137 protein with a KD value of 1*10<-9> M or less. The antibody or the antigen-binding fragment thereof has strong specific recognition and binding ability to the CD137 protein, and can activate a NF-[kappa]B signaling pathway, promote the proliferation of PBMC cells and / or induce immune cells to secretecytokines. The invention also provides an application of the antibody or the antigen-binding fragment thereof in preparation of drugs for treatment or prevention of cancer or autoimmune diseases.

Aspects of the invention relate to methods for measuring the binding constant of a lipophilic or amphiphilic molecule acceptor for a lipophilic or amphiphilic molecule. Methods involve rapid, cell-free competition assays including a labeled lipophilic or amphiphilic molecule and nanoparticle.

The present invention relates to the identification and cloning of a novel neutralizing human monoclonal antibody to the Respiratory Syncytial Virus. The invention provides such antibodies, fragments of such antibodies retaining RSV-binding ability, chimeric antibodies retaining RSV-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Finally, the invention provides for diagnostic and therapeutic methods employing the antibodies and nucleic acids of the invention.

The invention discloses a silane-nanoceramic composite surface conditioning agent. The silane-nanoceramic composite surface conditioning agent is used for surface treatment before metal electrophoresis coating, and 1L of the composite surface conditioning agent comprises 100-100000mg of amino silane or epoxy silane, 1-10000mg of dispersible nanoparticles, 10-50000mg of a zirconium compound or a titanium compound, and the balance of water. The silane-nanoceramic composite surface conditioning agent does not contain phosphorus or other harmful elements, and has pollution to environment in the use process; and compared with present phosphorus-free surface conditioning agents allowing a treated metal to have a thin film on the surface and have poor physical bonding ability with an electrophoresis coating, the silane-nanoceramic composite surface conditioning agent allows a treated metal to have a thick film on the surface in order to enhance the physical binding ability with the electrophoresis coating, so the corrosion resistance and the throwing power of the electrophoresis coated metal are greatly improved.

A method for removing a serum albumin from a mixture of other compounds by contacting said mixture with a ligand a) having affinity for and enabling selective binding of the serum albumin and b) being attached to a base matrix insoluble in the aqueous media used or being possible to attach to such a matrix after having become bound to the serum albumin, characterized in that said ligand is derived from an albumin binding bacterial cell surface receptor and that the ligand lacks the IgG-binding and / or alpha2-macroglobulin-binding ability found in native forms of these type of bacterial receptors. An albumin-binding ligand derived from a cell surface bacterial receptor and attached covalently to a carrier matrix, characterized in that the ligand is monovalent with respect to ability to bind a serum albumin. A method for removal of serum albumin from a sample that is to be assayed for non-serum albumin components. The characteristic feature is to subject the sample to affinity adsorption by an albumin-binding ligand derived from an albumin-binding receptor.

The present invention relates to monoclonal antibodies that bind or neutralize dengue type 1, 2, 3, and / or 4 virus. The invention provides such antibodies, fragments of such antibodies retaining dengue virus-binding ability, fully human or humanized antibodies retaining dengue virus-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Additionally, the invention provides for prophylactic, therapeutic, and diagnostic methods employing the antibodies and nucleic acids of the invention.

Titanium oxide-antibody conjugated particles are disclosed, which are provided with selective binding ability without loss of dispersibility and catalytic activity by modifying titanium oxide conjugated particles, dispersed in a water-based solvent by a water-soluble polymer, with an antibody via a linker molecule bound without changing the nature of the water-soluble polymer. The present invention is an antitumor agent, comprising titanium oxide-antibody conjugated particles, wherein a linker molecule is bound to the titanium oxide surface of the titanium oxide conjugated particles, dispersed in a water-based solvent by a water-soluble polymer, via at least one functional group selected from a group consisting of a carboxyl group, an amino group, a diol group, a salicylic acid group, and a phosphoric acid group, and wherein the titanium oxide conjugated particles are further modified with an antibody via the linker molecule. This antitumor agent is concentrated in the affected area and can be utilized as an agent for diagnosis or for treatment in combination with ultrasonic irradiation.

The present invention relates to a anti-ultraviolet superfine polyester fibre and its preparation process characterized by that the weight composition is 98-70 parts of polyester and 2-30 parts of surface coupled anti-ultraviolet additive, the titer of the fibre is 0.3-0.7dtex, the grain size of the anti-ultraviolet additive is 20-200nm. In this invention, because the anti-ultraviolet additive is treated with coupling agent to increase the additive amount, the anti-ultraviolet superfine polyester has not only remarkable ultraviolet resistance but also better binding ability with parental material and excellent mechanical property, providing large application field and significant social and economical benefit.

The invention discloses a monolithic integrated surface emitting semiconductor laser based on a super surface micro-nano structure. A super-surface micro-nano structure is combined with the active medium of semiconductor laser, the cluster coherent oscillation of the resonant unit inside the super-surface can be excited when the active material emits light in the active region. This local oscillation has high optical field binding ability and can provide positive feedback for the active layer to realize lasing. At the same time, by optimizing the structure of the super-surface micro-nano, we can control the far-field distribution characteristics of the beam, such as directional angle, phase, polarization, mode and so on. The laser structure comprises a substrate, a low refractive index layer, an active layer and a super-surface micro-nano structure. The preparation method comprises design and preparation of a light field confinement layer, a gain medium and the super-surface structure.Through the invention, the light field can be strongly localized in the near field, the far field distribution can be regulated by the arrangement mode of the structural unit, and the light field canbe directly regulated.

The invention which belongs to the technical field of structured catalysts and application technologies thereof concretely relates to a high intercrystal poriness zeolite coating material on the surface of a porous silicon carbide carrier and a preparation method thereof. The porous silicon carbide carrier involved by the material has a macroscopic porous structure, for example a foamy structure,and a honeycomb structure. The zeolite coating has a high intercrystal poriness. According to the preparation method, the surface of the porous silicon carbide carrier is modified by a colloidal zeolite guiding agent to realize the preferred growth of zeolite crystal on the surface of the porous silicon carbide carrier; and the alkalinity of a secondary growth solution, the concentration of nutrients, and the addition of alkali metal ions are controlled to control the intercrystal poriness of zeolite. The coating material has the advantages of high intercrystal poriness, small zeolite crystaldimension, good molecular diffusion ability, large zeolite load capacity, adjustable silica-alumina ratio of the zeolite crystal and zeolite coating thickness, and good binding ability of the zeolitecrystal with the surface of the porous silicon carbide carrier. The structured catalyst which is in favor of mass and heat transfer reinforcement has wide application prospects in fields of catalysis, adsorption, separation and the like.

Disclosed is a crystalline human CR2 protein in complex with C3d, and the three dimensional structure of the crystalline complex. Also disclosed are methods of use of the structure, particularly for structure-based identification of compounds that bind to CR2 and inhibit or enhance the binding of CR2 to a natural ligand, that bind to CR2 and agonize or antagonize the receptor, that bind to CR2 and inhibit or enhance CR2 dimerization, or that use the C3-binding ability of CR2 as a drug delivery vehicle. Also disclosed are therapeutic compounds obtained by such methods and uses for such compounds.