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Triiodothyronine quantitative detection kit and preparation method thereof

A technology of thyronine and triiodothyroid, which is applied in the field of in vitro immunoassay, can solve the problems of no biological activity, etc., and achieve the effects of improved detection performance, stable process, and high coupling efficiency

Active Publication Date: 2011-01-19
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

20% of T3 in the blood is directly synthesized by the thyroid gland, and the other 80% of T3 is produced by removing one molecule of iodine from T4. Anti-T3 can also be formed at different positions of deiodination, but anti-T3 has no biological activity

Method used

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  • Triiodothyronine quantitative detection kit and preparation method thereof
  • Triiodothyronine quantitative detection kit and preparation method thereof
  • Triiodothyronine quantitative detection kit and preparation method thereof

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Embodiment Construction

[0037]The triiodothyronine quantitative detection kit of the present invention comprises a magnetic particle suspension coated with a triiodothyronine structural analog (diiodothyronine, T2)-gelatin, the suspension The particle size of the magnetic particles used in the method is about 0.8-1.5um, and the surface contains carboxyl active groups with a concentration of 20-30ueq / g. The carboxyl groups are activated by an activator, and then combined with the amino groups of diiodothyronine-gelatin Connect to form a stable peptide bond; use hormone-free serum as the matrix, add triiodothyronine pure product to prepare triiodothyronine series calibrator; combine horseradish peroxidase with mouse anti-tri-iodothyronine Horseradish peroxidase-labeled triiodothyronine antibody linked to iodothyronine monoclonal antibody; dissociation agent for Tris-NaCl buffer containing ANS; enzymatic chemiluminescent substrate, enhancer Luminescent substrate A solution composed of amino acid and ami...

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Abstract

The invention discloses a triiodothyronine quantitative detection kit which comprises magnetic particle suspension coated with diiodothyronine-gelatin, a triiodothyronine series calibrator, a triiodothyronine antibody labelled by horseradish peroxidase, a dissociation agent, a chemiluminescent substrate A, a chemiluminescent substrate B and wash concentrate. The invention further discloses a preparation method of the kit. The invention has the advantage that a chemical structural analogue of hormone is coated by a method for coating an antigen analogue with a labelled antibody, and the analogue and the detected hormone have identical immune correlation, thus realizing specific binding of the analogue and the antibody of the hormone; but the binding capability of the analogue with thyroid-binding protein (THBP) is significantly lowered, thus reducing influence of the binding protein on a measuring system to a large extent. The kit has greatly improved sensitivity and precision, greatlyenlarged detection range, greatly shortened reaction time, enhanced product properties and lowered product cost.

Description

technical field [0001] The invention relates to in vitro immune detection, in particular to a triiodothyronine quantitative detection kit, and also relates to a preparation method of the kit. Background technique [0002] Triiodothyronine (3,5,3'-triiodothyronine, T3) is an iodotyrosine with a molecular weight of 651, which is an important thyroid hormone like thyroxine (T4) . Iodine and tyrosine are the main raw materials for the synthesis of thyroid hormones. Thyroglobulin is the place and raw material for iodination. The iodination of tyrosine and the coupling of iodotyrosine are carried out here. When T3 is hydrolyzed , T4 is secreted into the blood, 90% of the secretion is T4, and 10% is T3. 20% of T3 in the blood is directly synthesized by the thyroid gland, and the other 80% of T3 is produced by removing one molecule of iodine from T4. Anti-T3 can also be formed at different positions of deiodination, but anti-T3 has no biological activity. The half-life of T3 is 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/577G01N33/535
Inventor 高晓丹陈晓玲刘保鑫付光宇渠海马建军项立红吴学炜苗拥军
Owner AUTOBIO DIAGNOSTICS CO LTD
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