52 results about "Thyroglobulin" patented technology

Disclosed herein are novel approaches to thyroid cancer therapy. These approaches include methods to enhance thyroid specific gene expression, for example methods to enhance expression of thyroglobulin and / or the Na<+> / I<-> symporter in thyroid cancer cells. Enhanced expression of thyroid-specific genes promotes cellular differentiation and reduces biologically aggressive behavior such as invasion and metastasis. In addition, enhanced expression of thyroglobulin and / or the Na<+> / I<31 > symporter increases the ability of thyroid cancer cells to concentrate iodine or iodide, thereby making the cells more susceptible to radioactive iodine therapy. Also disclosed herein are methods for detecting thyroid neoplasms in a subject, by administering a therapeutically effective amount of a histone deacetylase inhibitor, administering a detectable agent whose uptake or concentration in thyroid cells is increased by administration of the histone deacetylase inhibitor, and detecting the detectable agent.

The present invention provides a human thyroglobulin antibody magnetic separation enzymatic chemiluminescent immunoassay method, belonging to the field of immunodetection and analysis technology. Said method includes the following steps: making antigen human thyroglobulin be connected with Fe3O4 mincrosphere surface as solid phase reagent, the diameter of Fe3O4 microsphere is 0.1-5.0 microns, after the self-body antibody human thyroglobulin antibody being in the sample is trapped and two antibodies are labeled by enzyme reagent, the solid phase-antigen-antibody-enzyme-labeled two-antibody sandwiched immune complex can be formed.

Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.

The invention relates to a magnetic particle chemiluminescence immunoassay method for human thyroglobulin antibodies (TGAb) and belongs to the technical field of immunoassay. TG antigens marked by fluorescein isothiocyanate (FITC) and human IgG antibodies marked by alkaline phosphatase are combined to form an antigen-antibody-second enzyme-labeled antibody sandwich immune complex with a sandwich-like structure. Then, magnetic particles connected with FITC antibodies are added, the antigen-antibody complex is connected to the magnetic particles through specific combination of the FITC antibodies and the FITC and directly deposited in an external magnetic field, and the complex formed by immune reaction can be separated from the other non-combined substances without centrifuging. A kit combines chemiluminescence with the magnetic particles to provide a reaction system close to a homogeneous phase. Compared with the prior art, the kit has the advantages of higher sensitivity, wide linear range, quickness and the like; the cost of a product is greatly reduced; and the kit has a broad application prospect on the aspects of clinical examination and the like.

The present invention is directed towards the synthesis and / or use of a mixture of analyte capture reagents comprising antibodies and a non-immunological reagent provided on a solid phase assay, selected to screen for very high and low levels of an analyte. The non-immunological reagent comprising a PC-conjugate that contains multiple copies of covalently coupled phosphorylcholine (PC) moieties, particularly towards a phosphorylcholine-thyroglobulin conjugate assaying for C-reactive protein (CRP), a known inflammatory marker. The mixture of capture reagents function to eliminate hook effect (i.e. false negatives) when CRP is present in sample at high concentrations.

Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.

The invention discloses a reagent kit and method for detecting a thyroglobulin antibody. The reagent kit contains a first reagent, a second reagent, a magnetic separation reagent, a chemical light-emitting substrate, a calibration article, a quality control article and cleaning liquid. The first reagent is a biotin reagent marked by thyroglobulin. The second reagent is a thyroglobulin enzyme combination reagent. The magnetic separation reagent is a superparamagnetic nanometer magnetic particle reagent marked by streptavidine, and the reagent kit is adopted for detecting the thyroglobulin antibody. Due to the mode, the defects that radioimmunoassay is high in pollution, the period of validity is short, common microwell plate enzyme immunoassay precision is poor, and sensitivity is poor are overcome, an alkaline phosphatase enzyme catalysis chemiluminescence system, a magnetic particle separation system and an unique antigen and antibody coupling technology are used, and the sensitivity, the precision, the stability, the validity period, the safety and the environment friendliness of the method are greatly improved.

The invention discloses a photo-thermal release signal enhanced type thyroglobulin electrochemiluminescence immunosensor. According to a construction method of the sensor, a silicon-based titanium dioxide magnetic bead is used as a substrate to fix a thyroglobulin antibody, mesoporous silica marked thyroglobulin adsorbing bipyridyl ruthenium is used as a signal probe, and a competitive immunosensor is constructed through specific recognition between an antigen and the antibody. An electrode for modifying the antibody is placed into a mixed solution containing different concentrations of free antigens and a certain amount of signal probes, so that the free target antigen and the signal probe compete to bind the antibody, and a certain luminous intensity can be generated by combining the electrode of the signal probe. Under the irradiation of an 808 nm infrared laser, a sensing interface is rapidly heated, the dipyridyl ruthenium adsorbed by the mesoporous silica is released, and a luminous signal is remarkably enhanced along with the temperature rise, so that signal amplification is realized. As the concentration of the free target is increased, the number of the signal probes combined with the antibody is reduced, the luminous intensity is also reduced, and an electrochemiluminescence analysis method for the thyroglobulin can be established on the basis of the phenomenon.

The invention discloses an immune colloidal gold test strip for detecting thyroglobulin and a preparation method thereof. The immune colloidal gold test strip includes a thyroglobulin detection gold test strip and preparation method thereof, wherein the immune colloidal gold strip, a gold labeled pad, a nitrocellulose membrane and a water absorption pad that are partly overlapped on a PVC base plate in order. The immune colloidal gold test strip is characterized in that: the gold labeled pad is coated with a colloidal gold labeled antithyroglobulin antibody, the nitrocellulose membrane is equipped with a separate detection line and quality control line, the detection line is coated with another antithyroglobulin antibody, the quality control line is coated with goat-anti-mouse IgG. The test strip provided by the invention has the advantages of simple preparation method, simple and convenient use, fast detection speed, high specificity, and qualitative and semi-quantitative detection of thyroglobulin, fully meets clinical needs, and has broad application prospects.

The invention discloses a thyroglobulin (TG) test kit, which is composed of a magnetic separation reagent, a reagent R1, a reagent R2, a standard substance, a quality control substance, a calibration substance, a cleaning concentrated solution, a dilution solution and a luminous substrate. The invention also discloses a preparation method of the kit. The kit provided by the invention combines the chemiluminescence technique and immune magnetic particles, and provides a reaction system close to a homogeneous phase. Compared with existing ELISA (enzyme-linked immuno sorbent assay) techniques, the kit and the method provided by the invention have the advantages of higher specificity and sensitivity, short detection time, simple operation mode, and accurate and reliable detection result, and greatly lower the product cost.

The invention discloses a thyroglobulin chemiluminescence detection kit and a preparation method thereof. The kit includes: magnetic particles coupled with thyroglobulin capture antibody, acridine ester-labeled thyroglobulin detection antibody, thyroglobulin calibrator solution, cleaning solution, chemiluminescence pre-excitation solution A, and chemiluminescence excitation solution B . The kit of the invention uses the magnetic separation chemiluminescence technology as the detection method, and combines the acridinium ester labeling technology at the same time. Compared with the prior art, the kit has the advantages of high sensitivity, fast and convenient detection, high accuracy, good repeatability, strong specificity and the like. .

The invention relates to a kit which uses enzyme to catalyze a chemiluminescent substrate, the absorbance of a chromogenic substrate in enzyme immunoassay is replaced by a light signal which is generated through detecting the chemiluminescent substrate and has the same absorbance as the chromogenic substrate, the sensitivity of the detection light signal is higher than the absorbance of the detection chromogenic substrate and the detection light signal provides specific, rapid and reliable basis for the diagnosis of thyroglobulin. The invention provides a kit for conveniently, rapidly, sensitively and stably detecting thyroglobulin and a preparation method thereof.

The invention provides an anti-human thyroglobulin monoclonal antibody. The monoclonal antibody has the characteristics of high specificity and high humanization degree after being combined with the human thyrogliobulin antigen. The invention also provides an anti-human thyroglobulin monoclonal antibody immunoglobulin, a fragment and an immunoconjugate thereof as well as a pharmaceutical composition including the immunoglobulin, the fragment or the immunoconjugate. The invention also provides a detection kit for detecting the human thyrogliobulin.

The invention belongs to the technical field of medical biology, and particularly relates to a thyroglobulin enzyme linked immunosorbent diagnostic kit, which is formed by a thyroglobulin polyclonal antibody-coated plate, a contrast buffer solution, an enzyme labeling solution, washing liquid, a developing solution, a substrate solution, a thyroglobulin standard substance and a stop solution. The thyroglobulin enzyme linked immunosorbent diagnostic kit has high sensitivity, high specificity, high accuracy, and favorable stability, the expiry date of the kit at the room temperature is 1 year, the expiry date of the kit at the temperature of 4 DEG C is 3 years, and the storage life of the kit is remarkably prolonged.

Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.

The invention relates to a test strip for detecting human thyroglobulin, which comprises a bottom plate, and a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and a water absorption pad which are arranged on the bottom plate and connected in sequence; a detection line is arranged on the nitrocellulose membrane; the colloidal gold combination pad contains colloidal gold particles labeled by a combined antibody; a capture antibody is anchored in the detection line, and the binding antibody and the capture antibody can specifically recognize and bind thyroglobulin and do not interfere with each other. The colloidal gold test strip provided by the invention is convenient and rapid to use, can complete a chromatographic reaction within 10 minutes, is used for quantitative detection, and helps a doctor to judge whether the Tg content in peripheral blood exceeds a normal value range or not within a short time. The semi-quantitative detection test strip can be used by a patient, and daily self-monitoring of the patient is facilitated. The bifunctional test strip can be used for removing test samples with Tg content in a normal range, so that the workload of clinical detection is greatly reduced.

The invention discloses novel application of a rapid immunodetection system to an operation. According to the novel application of the rapid immunodetection system to the operation, whether cervical lymph node metastasis of thyroid cancer exists or not is identified by detecting the content of thyroglobulin. The novel application of the rapid immunodetection system in the operation comprises the following specific operation steps that a, tissues of regional lymph nodes of a thyroid are punctured; b, an eluent for puncturing the tissues is prepared; c, quantitative detection treatment of the thyroglobulin (TG) is directly carried out on the prepared eluent through the rapid immunodetection system; and d, whether thyroid cancer metastasis of the regional lymph nodes exists or not is identified according to the TG detection result obtained by the rapid immunodetection system, wherein in the step c, the eluent is dripped to TG test paper, then the detection line and the quality control line on the TG test paper are scanned through a detector of the immunodetection system, and parameter calculation treatment is carried out through a processing unit arranged inside the detector so as toobtain the content of the thyroglobulin (TG) in the tissues. The novel application of the rapid immunodetection system to the operation has the characteristics of being convenient and rapid to operate, and operation can be directly performed beside an operating table during operation.

The invention discloses a kit for measuring the content of thyroglobulin (Tg) in human serum by a magnetism particulate chemiluminescence method. The kit comprises a calibration product, a quality control product, an anti-reagent, a magnetic particulate reagent and a luminescent substrate, wherein anti-reagent fluorescein isothiocyanate (FITC) labeled thyroglobulin is coated with antibody and alkaline phosphatase-labeled thyroglobulin labelled antibody; the magnetic particulate reagent is a magnetic particulate and sheep anti-FITC connection. A chemiluminescence technology is combined with immune magnetic particulates, so that a reaction system close to a homogeneous phase is provided; moreover, a one-step reaction mode is adopted, so that the detection sensitivity and precision are greatly enhanced, the detection range is enlarged, the reaction time is greatly shortened, and the time from the start of sample adding to a detection result is less than 35min, being remarkably faster thanthe same kind of kit; moreover, a plurality of samples can be measured at the same time on a fully-automatic chemiluminescence instrument, high-throughput and rapid determination of the thyroglobulinis realized, high accuracy and high specificity are achieved, and the precision and detection efficiency are greatly improved.

The invention discloses a kit for detecting a thyroglobulin antibody and a subtype thereof, and belongs to the technical field of biological medicines. The kit comprises a < 125 > I labeled human thyroglobulin tracer agent, a streptavidin agarose gel reagent, a biotinylated anti-human IgG subtype monoclonal antibody, protein A agarose and protein G agarose. On the basis of an existing radioactive ligand detection method, core steps are replaced by combining upstream and downstream principles, the streptavidin agarose gel reagent and the biotinylated anti-human IgG subtype monoclonal antibody are introduced for the first time, a core technical platform is created again for subtype typing of the antibody, and the method has the advantages of high specificity, high sensitivity and high sensitivity. And then the biotinylated anti-human IgG subtype monoclonal antibody is combined with the TGAb IgG subtype, so that the detection of the TGAb IgG subtype is realized, and the upgrading of a technical platform and the breakthrough of a TGAb IgG subtype detection technology are successfully realized.

The invention discloses a kit for determining the content of thyroglobulin antibodies (TGAb) in human serum by virtue of a magnetic particle chemiluminiscence method. The kit comprises a calibration product, a quality control product, a resistance reagent, a magnetic particle reagent and a luminescent substrate, wherein the resistance reagent contains a fluorescein isothiocyanate labeled thyroglobulin coating antigen and an alkaline phosphatase labeled thyroglobulin coating antigen; and the magnetic particle reagent is a conjugate of a magnetic particle and a goat-anti FITC. According to the kit, a chemiluminiscence technique is combined with immune magnetic particles, an approximately homogeneous reaction system is provided, and a one-step method reaction mode is adopted, so that the detection sensitivity and the precision are greatly improved, the detection range is expanded, the reaction time is greatly shortened, the time period from the starting of sample feeding to the acquiringof a detection result is less than 35 minutes and is obviously shorter than similar kits; and multiple samples can be simultaneously determinate on a full-automatic chemiluminescence device, so that the high-flux rapid determination of the thyroglobulin antibodies is realized, the accuracy is high, the specificity is strong, and the precision and the detection efficiency are greatly improved.

The invention relates to a kit for detecting a natural bispecific antibody resisting thyroid peroxidase (TPO) and thyroglobulin (Tg), and belongs to the field of clinical laboratory science. Through detection on a patient serum, a disease control serum and a healthy control serum, the invention finds that detection rate of the anti-TPO and anti-Tg bispecific antibody in the patients is significantly higher than the control group, thereby showing statistical significance. The invention has the advantages that the detection kit described in the invention provides a simple and practicable detection mode for bispecific antibody, and the kit can be applied to detection of all kinds of bispecific antibodies.

The invention relates to a distant metastasis analysis method for differentiated thyroid carcinoma (DTC), and relates to a distant metastasis analysis system for the DTC. The distant metastasis analysis system comprises a data receiving module for receiving measurement data, a request calculation module for judging whether or not the data is accordant with calculation processing according to a set measurement threshold value, a data calculation module for calculating data, a processing module for performing processing according to a calculation result, and a display module for displaying analysis information. A group of sTg (stimulated thyroglobulin) dynamic change relevant parameters before 131I treatment is calculated in order to eliminate the influences of residual thyroid tissues before successful thyroidectomy through 131I after surgery on the sTg level, and good reference information is provided for the increase of the early diagnosis accuracy of a DTC distant metastasis patient before 131I treatment. Compared with an existing clinically-applied method for analyzing the distant metastasis state of the patient with a single sTg level analysis, the method disclosed by the invention has the advantage that the analysis accuracy and specificity are improved.

The invention discloses the application of ursodesoxycholic acid or isomers, derivatives, medicinal salts or prodrug molecules thereof to preparation of medicines for treating and / or preventing thyroid diseases. The invention further discloses a pharmaceutical composition comprising the ursodesoxycholic acid. The ursodesoxycholic acid disclosed by the invention can achieve clinical effects of effectively lowering levels of thyroid peroxidase antibodies and thyroglobulin antibodies of patients suffering from hashimoto thyroiditis, obviously reducing thyroid nodules, improving diffuse swelling of the thyroid gland, avoiding abnormal thyroid functions and preventing thyroid cancer, is expected to become a drug for treating clinical thyroid diseases and has a very wide application prospect.

The invention discloses fluorescence immunochromatographic test paper for rapidly detecting human thyroglobulin (Tg) so as to rapidly identify thyroid papillary carcinoma lymphonodi cervicales metastasis. According to the test paper, a double-antibody sandwich method and a fluorescence immunochromatography technology are adopted to detect human Tg; and antibodies are prepared through specific antigen epitope peptides. With the method adopted, human Tg in an object to be detected can be rapidly and accurately detected. The test paper has the advantages of simple, convenient and rapid operation, wide detection range, high specificity and high sensitivity.

The invention relates to a compound with a therapeutic effect for thyroiditis, a stereoisomer, prodrug or pharmaceutically acceptable salt, pharmaceutical composition, preparation method and application of the compound. Experimental results show that the compound provided by the invention can improve the situation that the weight of mice with hashimoto thyroiditis induced by the high-iodine waterdescends obviously, and thyroid peroxidase antibodies (TPOAb) and thyroglobulin antibodies (TGAb) increase greatly; and the experimental results show that the compound provided by the invention has good therapeutic effect on thyroiditis, particularly hashimoto thyroiditis. The therapeutic effect of the compound provided with the invention is even more remarkable compared with the therapeutic effect of a positive control dexamethasone. Therefore, the compound provided by the invention is suitable for being used as a medicine for treating thyroiditis, particularly hashimoto thyroiditis.

The invention relates to a fluorescent test strip for quantitatively detecting thyroglobulin and a preparation method of the fluorescent test strip. The test strip sequentially comprises a PVC plate,a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad from bottom to top, wherein the combination pad is coated with an anti-thyroglobulin antibody labeled by nano quantum dot fluorescent microspheres, the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with another anti-thyroglobulin antibody, and the quality control line is coated with goat anti-mouse IgG. The preparation method is simple, steps easy to operate, the prepared fluorescent test strip can be used for rapidly detecting the Tg valueof thyroglobulin of suspicious lymph nodes on site; the test strip can also be used for lymph node fine needle puncture, elution and Tg value measurement of preoperative, intraoperative and postoperative follow-up visits, and assists in clear diagnosis of suspicious neck lymph node thyroid papillary cancer metastasis. The test strip provided by the invention is simple and convenient in use method,high in detection speed and strong in specificity.

The invention discloses a sargassum thunbergii lectin and a preparation method thereof, wherein the preparation method of the sargassum thunbergii lectin is characterized by selecting sargassum thunbergii as a raw material, carrying out lyophilization, pulverization, immersion, centrifugation, ammonium sulfate fractionation, DEAE-52 ion-exchange chromatography and SephadexG-200 gel filtration chromatography, thus producing the sargassum thunbergii lectin with a molecular weight of 17 kD. The sargassum thunbergii lectin of the invention can agglutinate a wide range of cells, has agglutinative functions for erythrocytes of rabbits, dogs, sheep, crucians, chickens and humans (A, B, AB), and the like, wherein minimum concentrations of agglutination reactions with rabbit and chicken erythrocytes are 4.4mg / ml and 0.55 mg / ml respectively, and shows hemagglutinin activity for rabbit erythrocytes even after heating treatment at 100 DEG C for 30 min (the activity decreases by 75%). A sugar inhibition experiment shows that the sargassum thunbergii lectin is only inhibited by glycoproteins as bovine thyroglobulin and gamma-globulin, and the minimum inhibition concentrations are 1.25 mg.mL<-1> and 2.5 mg.mL<-1> respectively.

Disclosed are a method and a reagent for measuring thyroglobulin, whereby it becomes possible to measure the amount of thyroglobulin more accurately by a single test without being affected by the interference with an anti-thyroglobulin antibody, and it also becomes possible to use an antibody having low resistance to acidifying agents and surfactants in immunoassays. A method for measuring thyroglobulin in a sample isolated from a living body by an immunoassay comprises a pretreatment step of mixing the sample isolated from the living body with a pretreatment solution containing an alkaline substance.