An enzyme-linked detection kit for detecting VEGF. A kit body contains an enzyme-linked reaction plate coated by a VEGF acceptor and a monoclonal antibody, an enzyme conjugate labelled by HRP and configured by antibody and a protective agent, a freeze-drying powder of the VEGF protein as a positive contrast, a protein freeze-drying powder as a negative contrast, a sample diluent, a PBST concentrated lotion, a chromogenic substrate configured by 3,3',5,5'-tetramethyl benzidine, 2MH2SO4 as a stopping solution and a seal plate gummed paper. The kit can be used in diagnosis and prognosis of cancer, angiopathy, diabetes, retinopathy and rheumatoid arthritis, and has advantages of accuracy, specificity, sensitivity, stability and convenience, etc.