The invention discloses a beta-human
chorionic gonadotrophin test kit (time-resolved fluoroimmunoassay) applicable for early-
pregnancy and mid-
pregnancy prenatal screening and a preparation method thereof. The kit comprises the following main components of an experimental
buffer solution, a concentrated washing solution, an enhancement solution, a reaction plate, a
standard product and a
quality control product of
filter paper dried blood spots and a
europium marker. The method for preparing the kit according to the invention comprises the following steps of: 1. preparing the experimental
buffer solution, the concentrated washing solution and the enhancement solution; 2.
coating the reaction plate; 3. preparing the
standard product and the
quality control product; 4. preparing the
europium marker; 5. separately loading; 6. sticking labels; and 7. assembling into a finished product. The invention has the characteristics that the accuracy and the sensitivity of a detected result are high, the stability is good, and a detecting method is economical, convenient and safe and has no traumatic property and the like. A
filter paper dried blood spot technology is applied to
prenatal screening work, which is beneficial to
blood specimen collection, storage and transportation and ensures the experimental reliability.