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112 results about "Horse serum" patented technology
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Horse serum, however, is not normally used to prepare toxoids, which are the main part of a vaccine. Horse serum unfortunately contains proteins foreign to the human body, which can cause severe hypersensitivity reactions.
Mycoplasma hyopneumoniae culture medium and preparation method thereof
ActiveCN102154167ARich in nutrientsIncrease the titer of live bacteriaBacteriaMicroorganism based processesPenicillinMycoplasma culture
The invention provides a mycoplasma hyopneumoniae culture medium and a preparation method thereof, belonging to the technical field of veterinary biology. The mycoplasma hyopneumoniae liquid culture medium comprises the components as follows: brain heart infusion, lactalbumin hydrolysate, PPLO (pleuropneumonia-like organism) broth, yeast extract powder, proteose peptone, sodium thiosulfate, Hank's liquid, sodium pyruvate, 0.1% phenol red solution, penicillin and deionized water. The preparation method comprises the following steps of: adding health horse serum before using, and adding agar into the liquid culture medium to obtain a solid culture medium of mycoplasma hyopneumoniae. The viable bacteria titer of the mycoplasma hyopneumoniae culture medium can reach 1*109CCU / ml-1*1010CCU / ml; the viable bacteria titer and the separation sensibility are far higher than those of the existing culture medium, and the mycoplasma hyopneumoniae is fast in growth speed and high in the separation sensibility; and the preparation method of the culture medium is simple in technology, strong in operability, and suitable for industrial large-scale production.
Owner:兆丰华生物科技(南京)有限公司 +1
Mycoplasma anatis culture medium and separation and purification method for mycoplasma anatis
ActiveCN105462884AHigh in nutrientsExtended storage timeBacteriaMicroorganism based processesBiotechnologyMycoplasma culture
The invention provides a mycoplasma anatis culture medium. The mycoplasma anatis culture medium is composed of a liquid culture medium and a solid culture medium. The liquid culture medium is prepared from deionized water, a 10% thallium acetate solution, PPLO broth, glucose, peptone, tryptone, 1% phenol red, a CMRL-1066 culture medium containing L-glutamine, inactivated horse serum, yeast leachate, penicillin, L-cysteine hydrochloride and nicotinamide adenine dinucleotide. Besides the situation that agar powder is added and phenol red is removed, other components of the solid culture medium are the same as those of the liquid culture medium. The culture medium is used for separating and purifying mycoplasma anatis, wherein a bacterial colony is separated from the solid culture medium, the separated bacterial colony is placed in the liquid culture medium to grow, then filtering, dilution and plate coating of liquid culture substances and purification of picked single colony multiplication are conducted, and the steps are repeated till purified mycoplasma anatis is obtained. The culture medium can promote growth of mycoplasma anatis, and the success rate of separation and purification of mycoplasma anatis is high.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Human source anti- tetanus exotoxin antibody and preparation method and use thereof
InactiveCN1594361AAvoid allergic reactionsAddressing issues that predispose to allergic reactionsAntibacterial agentsImmunoglobulins against virusesEscherichia coliHypersensitive response
The invention discloses a human source anti-tetanus exotoxin antibody (HTAT-Fab) and preparation method, comprising: constructing human immunity phage antibody bank, screening phage positive clone, further getting HTAT-Fab gene with a specific neutralization activity and high affinity. The gene can be expressed in the procaryotic cell such as E.coli, eukaryotic cell such as microzyme, or mammalian cell such as CHO, purifying to get highly purified HTAT-Fab with a strong tissue penetrability, a high affinity. The HTAT-Fab product can not only eliminate the allergic reaction generated by horse serum anti-tatanus antitoxin (TAT) (foreign protein), but also avoid the blood source for producing human tetanus immunoglobulin (HTIG) and the latent virus pollution.
Owner:北京明新高科技发展有限公司 +2
Recombinant pig FSH-CTP fusion protein as well as preparation method and application thereof
ActiveCN108676096AHigh purityImprove securityPeptide/protein ingredientsAntibody mimetics/scaffoldsAnimal scienceHalf-life
The invention provides a recombinant pig FSH-CTP fusion protein. The fusion protein means that a beta subunit of pig FSH is directly or indirectly linked to a beta subunit carboxy terminal peptide CTPof human, primate or equine mammalian chorionic gonadotropin, and an alpha subunit of pig FSH is bound to the beta subunit of pig FSH through Van der Waals force. The fusion protein can be prepared by using a eukaryotic expression system on the basis of a genetic engineering technology. Compared with pig pituitary FSH, the pig FSH-CTP fusion protein provided by the invention has a longer half-life and a better drug effect, and can replace pig pituitary FSH and pregnant horse serum gonadotropin in the current market in the field of animal propagation.
Owner:BEIJING VJT BIO CO LTD
Preparation method and application of cross-linked hydrogel for muscle stem cell culture
ActiveCN112778543AComplete mechanical propertiesNot easy to collapseCell culture supports/coatingSkeletal/connective tissue cellsFood materialEngineering
The invention discloses a preparation method and application of cross-linked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca<2+> are crosslinked through a physical crosslinking method to form double-network hydrogel with high mechanical strength, and then the hydrogel is coated with heparin and collagen through a dipping coating method, so that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, the extracted primary muscle stem cells are inoculated onto the hydrogel and cultured for 24 hours in a growth culture medium (79% of DMEM, 10% of FBS and 1% of double antibodies); and then the extracted primary muscle stem cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 days. By utilizing the hydrogel, the absorption of the muscle stem cells on nutrient substances can be enhanced, and the growth of the muscle stem cells is facilitated. The dual-network hydrogel has the potential to be used as a scaffold for muscle stem cell growth of stem cell culture meat.
Owner:汕头得宝投资有限公司
Mycoplasma bovis medium and preparation method
ActiveCN106399206AGrowth status can be judgedAvoid pollutionAntibacterial agentsBacterial antigen ingredientsPenicillinHydrolysate
The invention discloses a mycoplasma bovis medium and a preparation method thereof. The mycoplasma bovis medium comprises a basic medium and an auxiliary medium through mixing and is prepared from Friis media premix, sodium pyruvate, a fresh yeast extract liquid, glucose, phenol red, deionized water, L-glutamine, L-cysteine, lactoalbumin hydrolysate, transferrin, insulin, penicillin and a small amount of horse serum. The medium not only can reduce the use amount of serum, but also remarkably increases the titer of living bacteria of mycoplasma bovis and lays a foundation for preparation of high quality vaccines of mycoplasma bovis.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Chemiluminescent detection kit for bovine foot-and-mouth disease 3ABC antibody
ActiveCN106596966AQuick checkImprove solubilitySsRNA viruses positive-senseAntibody mimetics/scaffoldsEscherichia coliChemiluminescent immunoassay
The invention discloses a chemiluminescent detection kit for a bovine foot-and-mouth disease 3ABC antibody and belongs to the field of immunological detection. The chemiluminescent detection kit comprises a 96-well chemiluminescent immunoassay plate, an enzyme-labeled antibody, a serum diluent, a chemiluminescent substrate, a chemiluminescent enhancer and PBST washing liquid, wherein the 96-well chemiluminescent immunoassay plate is coated with 3ABC fusion protein, the enzyme-labeled antibody is a horseradish peroxidase labeled rabbit anti-bovine lgG antibody, the serum diluent comprises 0.01% of Tween-20, 1% of casein, 10% of horse serum and 1% of escherichia coli, the chemiluminescent substrate refers to a Luminol solution, and the chemiluminescent enhancer comprises IPP, H2O2 and Tween-20. Compared with commercially-available kit products, the chemiluminescent detection kit is high in sensitivity, higher in specificity and superior to other products in diagnosibility.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Mycoplasma ovipneumoniae low-serum medium and preparation method thereof
ActiveCN106479936AAvoid pollutionLong storage timeBacteriaMicroorganism based processesMycoplasma culturePenicillin
The invention discloses a mycoplasma ovipneumoniae low-serum medium, which consists of the following components: a PPLO broth, sodium pyruvate, phenol red, de-ionized water, lactose, tryptone, a fresh yeast extract, insulin, L-glutamine, L-cysteine, lactoalbumin hydrolysate, transferrin, penicillin and little horse serum; and the invention provides a preparation method of the mycoplasma ovipneumoniae low-serum medium. The mycoplasma ovipneumoniae medium provided by the invention has the following beneficial effects: a serum content is merely 5%, which is 1 / 4 of the serum content of a medium in the prior art, and the living bacterium titer of cultivated mycoplasma ovipneumoniae reaches 7*10<9> CCU / mL or above, which is significantly higher than that of the medium in the prior art. In comparison with the prior art, the mycoplasma ovipneumoniae low-serum medium provided by the invention has the characteristics of being low in serum content, rapid in growth and high in living bacterium titer.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Culture solution and method for inducing mesenchymal stem cells to differentiate into glomerular mesangial cells
InactiveCN103382458AFunction increaseThere are few types of useArtificial cell constructsSkeletal/connective tissue cellsZona glomerulosaCulture fluid
The invention discloses culture solution for inducing mesenchymal stem cells to differentiate into glomerular mesangial cells and an inducing method. The culture solution comprises a human platelet-derived growth factor-BB, all-trans-retinoic acid, collagen-IV and a basic medium, wherein the basic medium is a low-sugar DMEM (Dulbecco's modified eagle medium) containing 2% of horse serum and 5ng / ml of bFGF (basic fibroblast growth factor). The inducing method includes the steps: preparing the inducing culture solution; preparing the mesenchymal stem cells; coating a culture plate by the collagen-IV with the concentration of 5ug / cm<2>; inoculating the mesenchymal stem cells with the cell density of 5*103 / cm<2> into the coated culture plate and adding the inducing culture solution; changing the solution once every three days and performing inducing culture for seven days. The mesenchymal stem cells are jointly induced to differentiate into the glomerular mesangial cells by the aid of the human platelet-derived growth factor-BB, the all-trans-retinoic acid and the collagen-IV, inducing period is short, inducing efficiency is high, the glomerular mesangial cells formed by differentiation are high in functional activity, and transplantation therapy of kidney diseases such as kidney failure is facilitated.
Owner:HARBIN YIJIAYI REGENERATIVE MEDICINE TECH
Mycoplasma hyopneumoniae selective separation medium and preparation method thereof
ActiveCN104017856AThe preparation method is simple and easyReliable test resultsMicrobiological testing/measurementMicroorganism based processesBiotechnologyPenicillin
The invention belongs to the technical field of veterinary biology, and relates to a mycoplasma hyopneumoniae selective separation medium and a preparation method thereof. The mycoplasma hyopneumoniae selective separation medium comprises the main components of lactoalbumin hydrolysate, yeast extract powder, MEM (minimum essential medium), Hank 's solution, 0.1% phenol red solution, healthy horse serum, penicillin, deionized water, and specific growth inhibition serum. The mycoplasma hyopneumoniae selective separation medium can be used for the rapid separation and identification of mycoplasma hyopneumoniae, the preparation method is simple and feasible, experimental results are reliable, the separation time is shortened to 103h-122h, the time is shortened to 17%-23%, the separation success rate is as high as 75% above, and is more than 10 times of the separation success rate of mediums in the prior art.
Owner:SHANDONG LVDU BIO SICIENCE & TECH
Method for purifying myoblast derived from bovine fetus skeletal muscle tissue
ActiveCN106350480AEasy to purifyCell dissociation methodsSkeletal/connective tissue cellsAnti plateletEagle
The invention discloses a method for purifying myoblast derived from bovine fetus skeletal muscle tissue. The method is characterized in that the material used by the method is the longissimus dorsi muscle tissue of a 3-month-old bovine fetus, and reagents used by the method are horse serum, anti-fast muscle myosin heavy chain antibodies, collagenase, fetal bovine serum, a low-sugar Dulbecco modified Eagle medium, a cell-sorting buffer solution and anti-platelet derived growth factor receptor alpha antibodies.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Equine embryo-flushing solution as well as preparation method and applications of solution
ActiveCN102864122AEasy to useEasy to get materialsAnimal reproductionGerm cellsSodium lactatePenicillin
The invention discloses an equine embryo-flushing solution as well as a preparation method and applications of the solution. The embryo-flushing solution contains sodium lactate mixed liquor, penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum. The preparation method comprises the following steps: placing the sodium lactate mixed liquor in thermostat with the temperature of 37 DEG C for 0.5-6 hours for standby; and adding the penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum in the sodium lactate mixed liquor according to the component contents to obtain the embryo-flushing solution. The preparation method is suitable for the non-surgical embryo transfer technology of horses, cattle and donkeys. The equine embryo-flushing solution has the following beneficial effects that the solution has the advantages that raw materials are available, cost is low, preparation method is simple and use is convenient, is very suitable for the equine large-scale non-surgical embryo transfer and is beneficial to the popularization and application of the embryo transfer technology.
Owner:QINGDAO DERUI JUNFA BIOLOGY TECH
Preparation method of multivalence anti-venin yolk antibody
This invention relates to a preparation method of multivalent anti-snake venom chicken yolk antibody. It includes following steps:(1) collect different kinds of snake venom antigen; (2) preparation of chicken ovum of anti-snake venom chicken yolk antibody, and infuse snake venom to chicken for immunization, after 20 to 4 weeks continuously collect egg for 6 to 10 mouths.(3) extract multivalent anti-snake venom chicken yolk antibody. This product can replace horse serum for injection, and be able to developed as oral using drug which has extensive application prospects in prevention of snake bite.
Owner:广州医学院
Mycoplasma synoviae culture method
InactiveCN105838641AReduce incubation timeReduce mistakesBacteriaMicroorganism based processesMycoplasma synoviaeHorse serum
The invention provides a mycoplasma synoviae culture method including three steps of first-level seed breeding, seed solution preparation and bacterial liquid preparation. A used liquid culture medium comprises the components: PPLO broth with the concentration of 21.0 g / L, glucose with the concentration of 5.0 g / L, inactivated horse serum with the concentration of 200 ml / L, a 25% yeast extract liquid with the concentration of 200 ml / L, a 1% phenol red solution with the concentration of 1.0 ml / L, a 1% coenzyme I solution with the concentration of 30 ml / L, and a 1% L-cysteine solution with the concentration of 30 ml / L, wherein the pH value is adjusted to 7.6-7.8. The process method not only shortens the culture time of mycoplasma synoviae, but also reduces the procedures of multiple sampling and detection during culture, reduces the possibility of pollution in the culture process, reduces errors caused by manual operation, and has advancement compared with the prior art.
Owner:YEBIO BIOENG OF QINGDAO
Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum
ActiveCN101988075APromote growthImprove hydrogen production efficiencyMicroorganism based processesFermentationBiotechnologyClostridium pasteurianum
The invention provides a method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum. Under the sterile and anaerobic condition, anaerobic bacteria are used, a mixture consisting of peptone, beef extracts, yeast extracts, Na2HPO4, L-cysteine, L-cysteine hydrochloride monohydrate, diazoresorcinal indicators and distilled water is used as a culture medium, glucose is used as a fermentation substrate, a batch fermentation method is adopted for preparing the hydrogen, and the special anaerobic bacteria are clostridium pasteurianum JCM1408T. The culture medium consists of 10.0 to 15.0g of peptone, 2.0 to 3.0g of beef extracts, 5.0 to 10.0g of yeast extracts, 3.0 to 4.0g of Na2HPO4, 0.2 to 0.5g of L-cysteine, 0.2 to 0.5g of L-cysteine hydrochloride monohydrate, 1ml of 0.2 percent wt diazoresorcinal indicators, 40 to 60ml of horse serum and 1L of distilled water. The hydrogen can be continuously prepared.
Owner:SOUTHEAST UNIV
Cell clone with MyoG (myogenin) gene knock-in and MSTN (myostatin) gene knock-out prepared by Crispr/Cas9 technique
InactiveCN110257434AIncrease the number ofHigh meat yieldStable introduction of DNAPeptidesMyostatinFiber
The invention belongs to the technical field of genetic engineering and relates to a cell clone with MyoG (myogenin) gene knock-in and MSTN (myostatin) gene knock-out prepared by Crispr / Cas9 technique. The cell clone is characterized in that after being isolated and purified, the cell clone may be induced by 2% horse serum to differentiate into muscle fibers; skeleton satellite cells herein can merge into myotubes; an MSTN gene knock-out vector target is connected successfully, with no base mutations occurring; experimental group cells can differentiate into a certain quantity of myotubes; it is indicated that after MyoG gene is interfered, the cells still can differentiate into myotubes, while total myotube fusion rate is reduced and the form and quantity are significantly differed from those of a control group.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium and preparation method thereof
ActiveCN106591177AAvoid pollutionLong storage timeAntibacterial agentsBacterial antigen ingredientsHeterologousPenicillin
The invention discloses a mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium. The mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium comprises the following components of PPLO broth, sodium pyruvate, lactose, tryptone, fresh yeast extract, L-glutamine, L-cysteine, lactoalbumin hydrolysate, horse serum, penicillin, phenol red, and deionized water; and the invention also provides a preparation method. The mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium has the beneficial effect that the serum content of the mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium is only 5%, which is 1 / 5-1 / 4 of the serum amount of the mediums in the prior art; in addition, the prepared semi-finished product bacteria liquid titer can reach 1010 CCU / ml, which is obviously higher than that of the medium in the prior art. The low-serum high efficiency medium used for culturing the mycoplasma capricolum subsp. Capripneumoniae is in favor of mitigating allergic stress reaction of heterogenous serum on goat, increases the biological safety, increases the live bacteria titer, and reduces the production cost.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Aged rat brain vascular endothelial cell culture fluid
InactiveCN103045534AHigh purityPromote divisionArtificial cell constructsVertebrate cellsInsulin-like growth factorPlasma derived
The invention relates to an aged rat brain vascular endothelial cell culture fluid. On the basis of each 100 ml of DMEM (Dulbecco Modified Eagle Medium) culture medium, components including plasma derived serum (PDS), penicillin-streptomycin, L-glutamine, gentamicin, heparin, an alkaline fiber growth factor, an endothelial cell growth replenishing factor, vitamin C, a vascular endothelial growth factor, an insulin-like growth factor-1 and an endothelium growth factor are added. According to the aged rat brain vascular endothelial cell culture fluid, the PDS is adopted for replacing FBS (Fetal Bovine Serum), beef calf serum or horse serum in the traditional culture fluid to ensure that the purity of brain vascular endothelial cells is increased by 30 percent; and the vitamin C and puromycin are added to ensure that the brain vascular endothelial cells grow more rapidly and purely.
Owner:SHANDONG UNIV
Culture medium named MV06 for both endothelial and myocardiac cells
Owner:INSTITUT DE RECH & HEMATOLOGIE & TRANSPLANTATION
Culture medium for in-vitro culture of osteoblasts
InactiveCN105567629APromote growthReduce usageCulture processArtificial cell constructsSodium bicarbonateHorse serum
The invention discloses a culture medium for in-vitro culture of osteoblasts. The culture medium is characterized by being prepared from 10.0-15.0 g / L of a basal culture medium, 100-300 mg / L of tremella polysaccharide, 8-10 mL of serum, a proper amount of sodium bicarbonate for regulating the pH value to be 7.2-7.6, and the balance water added for achieving the constant volume of 1 L, wherein the basal culture medium is a DMEM or an alpha-MEM or RPMI-1640 or F12 or DMEM / F12, the purity of the tremella polysaccharide is larger than 75%, and the serum is fetal calf serum or calf serum or human serum or horse serum or sheep serum. The culture medium is used for in-vitro culture of osteoblasts. The culture medium has the advantages that growth of osteoblasts can be accelerated, and the use amount of the serum can be reduced.
Owner:SUZHOU PULUODA BIOLOGICAL SCI & TECH
Lower-serum and efficient culture medium of mycoplasma ovipneumoniae and preparation method of lower-serum and efficient culture medium
ActiveCN106635895AAvoid pollutionLong storage timeAntibacterial agentsBacterial antigen ingredientsHeterologousMycoplasma culture
The invention discloses a lower-serum and efficient culture medium of mycoplasma ovipneumoniae. The lower-serum and efficient culture medium is prepared from the following components: MEM, sodium pyruvate, glucose, Hank's liquid, fresh yeast leaching liquid, L-glutamine, L-cysteine, lactalbumin hydrolysate, calf thymus DNA (Deoxyribonucleic Acid), insulin, transferrin, penicillin, horse serum, phenol red and de-ionized water; the invention provides a preparation method of the lower-serum and efficient culture medium. The lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, has the beneficial effects that the content of the serum is only 5 percent and is 1 / 4 of the content of the serum in a culture medium in the prior art; the titer of mycoplasma ovipneumoniae semi-finished-product bacterium liquid prepared by the method reaches 10<10>CCU / ml and is obviously higher than that of the culture medium in the prior art. According to the lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, the sensitive stress response to a goat body, caused by heterologous serum, is alleviated, and the biosafety is improved; the titer of bacterium liquid of living bacteria is also improved and the production cost is reduced.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Establishing method of myosatellite cell system of bastard halibut during embryo period
ActiveCN108753703AOvercome dysfunctional issuesCultivate suitableCell dissociation methodsCulture processFunctional genesSeawater
The invention relates to a marine fish embryo cell culture technology, in particular to an establishing method of a myosatellite cell system of bastard halibut during an embryonic period. The establishing method comprises the following steps: carrying out in-vitro separation on an embryo during a bastard halibut kirschner capsule formation period, removing an egg membrane, dispersing cells, and carrying out primary culture and secondary culture, thus establishing the myosatellite cell system during the embryonic period. The form of the myosatellite cell system, established by the invention, ofthe bastard halibut is fusiform or spindle-shaped mononuclear cells; myofiber can be differentiated to form by culturing for 5 days or above within a single generation or carrying out induction of anexogenous factor (horse serum). The myosatellite cell system is capable of supplying a large amount of myosatellite cells, and GFD (Green Fluorescent Protein) transfection and cynoglossus semilaevisspleen and kidney necrosis virus challenge assay detection verify that the myosatellite cells can be normally transfected or infected, so that the myosatellite cell system not only can be directly used for researching functional genes related to muscle growth and development of fishes and providing research materials for exploring a molecular mechanism for induction, proliferation and differentiation of muscle cells of the fishes, but also is capable of providing a platform for improved research and application of muscle characters in bastard halibut breeding.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Extraction process of extracellular exosomes based on differential centrifugation
InactiveCN110669723ASolve the problem of relatively small adhesion strengthEasy to separateCell dissociation methodsArtificial cell constructsCells isolationCultured cell
The invention relates to the technical field of exosome extraction technology, and provides an extraction process of extracellular exosomes based on differential centrifugation. The culture for culturing HEK-293 cells is preferably a high-glucose DMEM culture medium, the problem that the adhesion strength of the HEK-293 cells is relatively small during the culture process can be effectively solved, so that the HEK-293 cells in the culture medium are produced at the same location, and the HEK-293 cells are isolated conveniently; in the culture process of the HEK-293 cells, 10% of horse serum isadded to the DMEM culture medium, a similar biological internal environment can be provided for cells cultured in vitro, the horse serum further contains several animal hormones and enzymes, and thedevelopment and growth of the HEK-293 cells can be promoted; and step S1, step S2 and step S3 are carried out on the HEK-293 cells before the differential centrifugation is performed, a supernatant extracted from the cells can be concentrated, the supernatant is collected after concentration, the purity of the exosomes in an extract can be improved to a certain extent, and the extraction effect isbetter.
Owner:赵凯
Culture medium for separation and purification of mycoplasma bovirhinis, preparation method and application of culture medium
ActiveCN106635865AIncrease success ratePromote growthBacteriaMicroorganism based processesAmpicillinHydrolysate
The invention discloses a culture medium for separation and purification of mycoplasma bovirhinis. Every 1000ml of culture medium comprises, by weight 10.5g of PPLO (pleuropneumonia-like organism) broth, 8,0g of cerebrocardiac leach liquor, 4.0g of sodium chloride, 0.05g of magnesium sulfate, 0.2g of potassium chloride, 0,09g of calcium chloride, 0.024g of disodium hydrogen phosphate, 0,03g of monopotassium phosphate, 5.0g of glucose, 5.0g of lactoalbumin hydrolysate, 60ml of 25% of fresh yeast leach liquor, 100ml of aseptic swine serum, 100ml of aseptic horse serum, 100mg of ampicillin and 1ml of 10% of thallium acetate. The invention further provides a preparation method and application of the culture medium. Compared with the prior art, the culture medium for separation and purification of the mycoplasma bovirhinis, the preparation method and the application of the culture medium have the advantages of high growing speed and titer and high mycoplasma bovirhinis separation success rate.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Extraction of immunoglobulin
InactiveCN1680446AImprove product appearanceHigh extraction purityImmunoglobulins against animals/humansPeptide preparation methodsAmmonium sulfateBlood serum
Owner:宋怀燕
Ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit
InactiveCN104988206AResolve detectionResolve accuracyMicrobiological testing/measurementMicroorganism based processesPenicillinArginine
Owner:姜洪波 +1
Haemophilus parasuis culture medium
InactiveCN112831428AGrowth does not affectFull of nutritionBacteriaMicrobiological testing/measurementBiotechnologyDipotassium phosphate
The invention discloses a haemophilus parasuis culture medium. The haemophilus parasuis culture medium consists of a basic culture solution and an auxiliary material, the auxiliary material is a mixture of nicotinamide adenine dinucleotide, a phenol red indicator, linkkinin and horse serum; each L of the basic culture solution comprises the following components: 15.0 to 17.0 g of tryptone; 3.0 to 5.0 g of plant peptone; 4.0 to 5.5 g of sodium chloride; 2.0 to 3.5 g of dipotassium phosphate; 2.0 to 3.5 g of glucose; and the balance of water. The haemophilus parasuis culture medium is rich in nutrient components, very suitable for rapid growth of haemophilus parasuis and capable of improving the separation rate of the haemophilus parasuis in a polluted material, phenol red is added into the culture medium to serve as an indicator, on one hand, growth of the haemophilus parasuis cannot be affected, on the other hand, change of acidity and alkalinity can be indicated, in the growth process of the haemophilus parasuis, glucose is fermented to produce acid, alkali in a culture solution can be neutralized, the color of phenol red is changed from red to yellow, and the growth condition of the bacteria can be judged according to the change of the color of the phenol red.
Owner:北京信得威特科技有限公司
Nutrient solution for detecting bacterial vaginosis, kit and detection method
InactiveCN106755279AQuality improvementEasy to operateMicrobiological testing/measurementBacterial vaginosisNutrient solution
The invention discloses a nutrient solution for detecting bacterial vaginosis. Each 100ml of nutrient solution contains the following raw materials: 0.1-1g of beef heart powder, 0.5-5g of tryptone, 0.1-2g of glucose, 0.1-1g of sodium chloride, 0.1-1g of bromocresol purple sodium, 5-20ml of horse serum, 0.0001-0.001g of neomycin sulfate and 0.0001-0.001g of nystatin. The invention further discloses a kit for detecting bacterial vaginosis and a detection method. The detection kit is stable in quality; the detection method is simple in operation and accurate in result. The kit has the functions of target cultivation and simultaneous detection of the sensitivity of a drug, the detection method is completed in one step within 6-24h, the success rate is high, the drug sensitivity result is reliable, clinical accurate diagnosis of the bacterial vaginosis and the drug sensitivity test can be met, a reference foundation is provided for a clinician in timely accurate diagnosis and reasonable use of the drug, and clinical abuse of antibiotics is avoided.
Owner:合肥百盛园生物药业有限公司
Normal temperature preserving liquid for equine animal embryo, and preparation method and application thereof
InactiveCN103749432AEasy to useEasy to get materialsDead animal preservationSodium lactateAnimal science
The invention discloses a normal temperature preserving liquid for equine animal embryos, and a preparation method and application thereof. The normal temperature preserving liquid includes a sodium lactate mixed solution, penicillin, streptomycin, and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum. The preparation method includes the steps of: placing the sodium lactate mixed solution in a thermostat at 37 DEG C for 0.5-6 h for standby; and adding penicillin, streptomycin and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum into the sodium lactate mixed solution in accordance with the component contents, so as to obtain the normal temperature preserving liquid for embryos. The preserving liquid is suitable for non-surgical method for embryo transplantation of horses, cattle and donkeys. The invention has the beneficial effects of convenience for material acquisition, low price, simple preparation and convenience for usage, is very suitable for scale production of non-surgical method for embryo transplantation of equine animals, and is favorable for popularization and application of embryo transplantation technology.
Owner:QINGDAO DERUI JUNFA BIOLOGY TECH +2
Method for inducing estrus synchronization and superovulation of female cat
InactiveCN111956366AWide variety of sourcesEasy to useAnimal reproductionAnimal husbandryObstetricsHCG - Human chorionic gonadotropin
The invention discloses a method for inducing estrus and ovulation of a female cat, and belongs to the technical field of animal artificial propagation. The invention provides the method for inducingestrus synchronization and superovulation of the female cat in order to solve the problem that estrus and ovulation are difficult to synchronize in artificial propagation of the female cat. The methodspecifically comprises the following steps: continuously feeding adult healthy female cats with altrenogest with a dosage of 0.01-20mg / cat / day for 10-20 days, stopping feeding the altrenogest for 2 to 3 days, injecting 10-1000IU of pregnant mare serum hormone into the muscle of the female cat, and intramuscularly injecting 10-1000 IU of human chorionic gonadotropin after 72-96 hours, so that thefemale cat can perform estrus synchronization and superovulation within a preset time. The key technical difficulty of artificial breeding of the domestic cat or the feline is solved.
Owner:NORTHWEST A & F UNIV
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