62 results about "Lactalbumin hydrolysate" patented technology

The invention provides the use of a whey protein and / or whey protein hydrolysate which stimulate the cellular release of the satiety peptides choleocystokinin and glucagon-like-peptide in the preparation of edible compositions. The edible compositions can be used to control body weight and have beneficial effects on satiety. Edible compositions are also provided.

The invention discloses a muting sensitive lactalbumin hydrolysate and a preparation method thereof. The method mainly comprises the following steps: dissolving condensed whey protein in the water, then carrying out denaturation under high temperature, carrying out hydrolysis by using the protamex composed of alkali protease, papain and flavourzyme, treating the lactalbumin hydrating solution with desalinization and drying to obtain the lactalbumin hydrolysate of the invention. The lactalbumin hydrolysate of the invention has high suppression ratio of Beta-lactoglobulin and Alpha- lactalbumin, can reduce or eliminate cow milk allergic reaction; furthermore, the lactalbumin hydrolysate can also produce other active materials, thus also having the functions of promoting immunity, being easy to digest and absorb and reducing blood pressure; and the lactalbumin hydrolysate has high security and no side effect, bitter taste or other bad flavors.

The invention provides a mycoplasma hyopneumoniae culture medium and a preparation method thereof, belonging to the technical field of veterinary biology. The mycoplasma hyopneumoniae liquid culture medium comprises the components as follows: brain heart infusion, lactalbumin hydrolysate, PPLO (pleuropneumonia-like organism) broth, yeast extract powder, proteose peptone, sodium thiosulfate, Hank's liquid, sodium pyruvate, 0.1% phenol red solution, penicillin and deionized water. The preparation method comprises the following steps of: adding health horse serum before using, and adding agar into the liquid culture medium to obtain a solid culture medium of mycoplasma hyopneumoniae. The viable bacteria titer of the mycoplasma hyopneumoniae culture medium can reach 1*109CCU / ml-1*1010CCU / ml; the viable bacteria titer and the separation sensibility are far higher than those of the existing culture medium, and the mycoplasma hyopneumoniae is fast in growth speed and high in the separation sensibility; and the preparation method of the culture medium is simple in technology, strong in operability, and suitable for industrial large-scale production.

The invention relates to alpine rhododendron tissue culture quick-propagation method. All the medium is prepared according to following material: medium for inducing explant: 1 / 4MS, MS ferric salt, VitB50.2mg, lactalbumin hydrolysate 300mg, sucrose 30g+Kt1.0mg / L; medium for propagating cluster sprout: 1 / 2MS, Kt0.5mg / L; medium for rooting: 1 / 2MS, NAA5mg / L, active carbon 0.5%. The method of alpine rhododendron tissue culture quick-propagation is characterized as test tube sprout provisional planting method adopting tender stem segment axillary bud culture method and 'two-step' method. The advantages of the invention lie in: the growth is not influenced by season, plant has strong resistance to stress, and the plant has big colorful flowers.

The invention, based on the character of nutrient content in breast milk, provides a infant formula milk powder constituted by reconstructed infant formula milk powder proteide. The main raw materials for preparing the reconstructed infant formula milk powder are as follows; fresh milk hydrolyzed by Neutrase enzyme with the degree of hydrolysis achieves 3 per cent to 12 per cent, lactalbumin hydrolysate, lactose, palm oil, soybean oil, low polysaccharide and one or several kinds of compound nutriment 1000 kg concentrated as dry matter. The invention utilizing the Neutrase enzyme to hydrolyze milk casein difficult for digesting by infant into micromolecule peptide and also replacing the whey mist used in traditional milk powder with the lactalbumin hydrolysate to reconstruct the infant formula milk powder proteide with the combination of the two technologies, thus improving the digestibility of milk protein, lowing the irritability of the milk protein and enabling the product has enhanced nutritive value and more easily being accepted by consumers, which is significant for practical application.

The invention relates to an intacted protein and short peptide compound type special-dietary nutritional emulsion for clinic patients. The nutritional emulsion is prepared from the following raw materials in parts by mass: 1-3 parts of sodium caseinate, 0.5-2 parts of lactalbumin hydrolysate, 0.5-1.5 parts of soybean peptide, 0.1-0.4 part of marine collagen peptide, 1-3 parts of peanut oil, 1-3 parts of medium chain triglyceride, 1-3 parts of sucrose, 1-3 parts of maltodextrin, 0.2-0.6 part of inulin, 0.2-0.4 part of polydextrose, 0.01-0.02 part of compound vitamins, 0.03-0.07 part of compound mineral substances, 0.05-0.15 parts of sucrose fatty acid ester, 0.05-0.15 part of distilled glycerin monostearate, 0.1-0.2 part of carboxymethylcellulose, 0.02-0.08 part of xanthan gum, 0.01-0.04 part of sodium alginate and 80-90 parts of water. The invention further discloses a preparation method of the special-dietary emulsion. The nutritional emulsion is good in stability and rich in nutrition and has the advantages that the malnutrition conditions of the clinic patients can be effectively improved on the base of reducing the medical cost.

The present invention relates to a process of preparing a whey protein hydrolysate from a WPI substrate having improved flavour, functionality and ACE-I inhibiting properties.

The invention provides a method for IBDV serum-free microcarrier suspension culture proliferation, comprising the following steps: 1) Acclimatization of Vero cells in serum-free culture; 2) IBDV virus propagation: inoculate the acclimatized cells into 0.25% whey The serum-free medium of the protein hydrolyzate was inoculated with the IBDV virus when the cells adhered to the microcarrier and started to grow, and the virus was harvested after 90 hours. The present invention domesticates the Vero cells introduced from ATCC, cultivates them with reduced serum, finally obtains Vero cells under the condition of serum-free microcarrier culture, improves the culture medium composition during IBDV virus proliferation at the same time, reduces the influence of exogenous viruses, and improves IBDV Virus titers under serum-free microcarrier culture conditions. The titer of the IBDV virus propagated by optimizing the virus propagation medium is higher than that of the IBDV propagated by the unoptimized medium and the IBDV virus titer propagated by chicken embryo fibroblasts; it is equivalent to the IBDV cultured by Vero cells with serum; the immunogenicity experiment shows that : The virus has strong immunogenicity and is suitable for production as a vaccine.

The invention provides a heat-resistant freeze-drying protective agent for an animal live vaccine. Each liter of the heat-resistant freeze-drying protective agent comprises the components by weight: 80-200g / L of lactose, 10-40g / L of lactalbumin hydrolysate, 5-20g / L of polyvinylpyrrolidone, 0-20g / L of mannitol, 5-20g / L of sodium glutamate, 5-15g / L of arginine monohydrochloride and 10-30g / L of hydrolyzed silk protein and the agent is prepared by autoclaved sterilization after water is added to dissolve the above components. The freeze-drying protective agent is mixed with a virus solution, and frozen and dried, so as to obtain the freeze-dried vaccine. The heat-resistant freeze-drying protective agent disclosed by the invention is simple in formula, easy to prepare, and applicable to large-scale production, and has a good protection effect on the vaccine. The prepared vaccine is safe and sterile, and stable in quality, and has the characteristics of being heat-resistant and long in preservation time, and the problems that the vaccine needs to be frozen at a low temperature in the transportation process, and is inconvenient to store and the like are solved. By adopting the heat-resistant freeze-drying protective agent provided by the invention, a porcine reproduction and respiratory syndrome vaccine and a pseudorabies live vaccine can be stored for 24 hours at 2-8 DEG C.

Our objective is to provide a novel lipid metabolism-improving reagent effective in preventing and treating hyperlipidemia and obesity and to provide this reagent in food, beverages, nutritional supplements, and fodders with the ability to improve lipid metabolism. The whey protein hydrolysate as the active ingredient in the lipid metabolism-improving reagent has a molecular weight distribution of 10 kDa or less, with the main peak between 200 Da to 3 kDa, average peptide chain length (APL) of 2-8, free amino acid content of 20% or less, antigenicity of 1 / 10,000 or less compared with that of β-lactoglobulin.

The invention discloses a preparation method of high-aroma type sachima, which comprises the following steps: evenly mixing flour, fresh eggs, a leavening agent, wheat gluten lactalbumin hydrolysate and water, fermenting, cutting into predetermined shape, deep frying in vegetable oil at 130-150 DEG C for 1-5 min, and then obtaining fried twisted dough sticks; and after boiling grape syrup to 100-106 DEG C, mixing the grape syrup with the fried twisted dough sticks, pressing flat, cutting, molding and obtaining the high-aroma type sachima. In the preparation method, by adding the wheat gluten lactalbumin hydrolysate, the aroma intensity of sachima can be significantly improved under the process conditions without affecting the color and luster of the product.

The invention provides a method for preparing lactalbumin hydrolysate by utilizing yak milk, and belongs to the field of milk product processing. The method for preparing the lactalbumin hydrolysate finished product comprises the following steps: degreasing the yak milk, preparing rennet casein, baking and crushing; hydrolyzing, inactivating enzyme, purifying, concentrating, drying and carrying out other steps. The method for preparing the lactalbumin hydrolysate has the advantages of short time as well as mild reacting condition, complete hydrolysis and high hydrolyzing rate by using bienzyme hydrolysis compared with acid and alkali hydrolysis.

The invention discloses alpha-lactalbumin hydrolysate and a preparation method and application thereof. The method for preparing the alpha-lactalbumin hydrolysate by performing enzymatic hydrolysis on alpha-lactalbumin comprises the steps of preparing an alpha-lactalbumin aqueous solution; adding flavourzyme into the alpha-lactalbumin aqueous solution for hydrolysis until the hydrolysis is 5 to 10 percent; and ending the hydrolysis. The alpha-lactalbumin hydrolysate prepared by the method has relatively high content of lysine, arginine, tryptophan and leucine and is used for preparing functional albumen powder; the content of branched chain amino acid, heparolysate and tryptophan in the functional albumen powder can be increased; the alpha-lactalbumin hydrolysate is favorable for quick digestion and absorption of protein by fitness and body building people and athletes; a nutrition requirement that a large amount of protein is supplemented within short time can be met; the burden on the lung function, which is caused by digestion, can be reduced; furthermore, due to a large amount of tryptophan, the sleepiness of special people can be improved after doing exercises, the appetite of the people can be adjusted, and the pressure can be controlled.

The invention relates to a method for separating and purifying polypeptide with tris ligand and high-concentration high-crosslinking degree agarose gel hydrophilic adsorption chromatography medium. By adopting the tris ligand and the medium which takes the high-concentration high-crosslinking degree agarose gel as base material, and taking a hydrophilic adsorption chromatography theory which takes hydrogen bond interaction as a characteristic, the method further separates and purifies high-purity polypeptide from biological tissue extractive and lactalbumin hydrolysate or polypeptide synthetical mixture, thereby having the advantages of high selectivity, simple purifying technology, high sample carrying capacity, high medium repeat utilization ratio, etc. The method overcomes the defects of complex steps, the low repeat utilization ratio of the reversed phase chromatography medium, hard scalization, low sample carrying capacity, bad biocompatibility, etc. The purity of the obtained polypeptide fraction is ensured with a reversed phase high-efficient liquid phase chromatography, and the sequence and the molecular weight are ensured by mass spectrum.

The invention provides a serum-free medium for culturing insect cells in a full suspension mode. The serum-free medium is prepared from a Grace's Insect Medium, a BFPM410 medium, 2-4 g / L of glucose, 1-3 g / L of lactalbumin hydrolysate, 1-3 g / L of PF68 and 2-5 g / L of yeast powder. The invention further provides a preparation method and application of the serum-free medium. As shown in experiments, the serum-free medium can be well used for culturing insect cells in the full suspension mode, the maximum cell proliferation concentration is 42.5*10<5> / mL when the serum-free medium is used for culturing Sf-21 cells, and the maximum cell proliferation concentration is 45.4*10<5> / mL when the serum-free medium is used for culturing Hi-five cells.

The invention relates to a hydrangea dedicated culture medium, which comprises the following components in parts by weight: 60 to 65 parts of MS basic mother liquor, 4 to 6 parts of glucose, 6 to 8 parts of lactalbumin hydrolysate, 4 to 8 parts of maize endosperm, 4 to 8 parts of tomato juice, 2 to 14 parts of coconut milk, 2 to 5 parts of indole butyric acid, 1 to 4 parts of naphthylacetic acid, 2 to 6 parts of ABT rooting powder, 5 to 15 parts of rare earth compounds, 5 to 10 parts of active carbon, and 30 to 35 parts of water. The provided culture medium is simple to prepare, the seedling emergence number is large, and technical support is provided for industrial seedling culture.

Belonging to the field of veterinary biological products, the invention discloses a mycoplaa hyopneumoniae selective isolation medium and a preparation method thereof. The mycoplaa hyopneumoniae selective isolation medium comprises the following components: brain heart infusion, PPLO broth, 10*Hank's solution, yeast extracted liquid, lactalbumin hydrolysate, an amino acid composition, glucose, penicillin, nystatin, 0.1% phenol red, inactivated pig serum, specific growth inhibiting serum and deionized water. The mycoplaa hyopneumoniae selective isolation medium provided by the invention has thecharacteristics of short isolation time, high isolation success rate, viable bacteria titer and isolation sensitivity far higher than those of the medium in the prior art, and is suitable for rapid growth of mycoplaa hyopneumoniae.

The invention provides a special medical food for patients in emergency states and a preparation method thereof. The main raw materials for producing one ton of the product mainly comprise the following components: 100-1500kg of yak milk, 100-2000kg of fresh milk, 200-500kg of whey powder, 50-200kg of refined vegetable oil, 1-100kg of medium chain triglyceride, 1-150kg of white granulated sugar, 1-120kg of lactose, 1-120kg of amino acid, 1-50kg of lactalbumin hydrolysate, 1-120kg of docosahexaenoic acid (DHA), 2-40kg of arachidonic acid (ARA) and 0.1-3kg of casein phosphopeptides. The special medical food is prepared by adding a proper amount of vitamins and minerals or other nutritional ingredients into the yak milk, fresh milk, whey powder, refined vegetable oil, medium chain triglyceride, white granulated sugar, lactose, amino acid, lactalbumin hydrolysate, DHA, ARA and casein phosphopeptides and then processing the raw materials through a physical method, so that the special medical food can meet the demands on various nutritional ingredients of the crowd suffering from malnutrition, protein deficiency, wounds with toxin accumulation and high catabolism, infection, operation and other stress states.

The invention relates to a special culture medium MB-HLS for the amplification culture of mycoplasma bovis and a preparation method of the culture medium MB-HLS. The special culture medium MB-HLS is characterized in that the protein content of the mycoplasma bovis is 1.61-1.83 mg / ml after the mycoplasma bovis is cultured for 72 hours through the culture medium MB-HLS prepared through methods, namely steam heating, stirring, filtration and sterilization by adding a right amount of trypsin, NaCl, MgSO4.7H2O, MgCl2.6H2O, Na2HPO4.2H2O, KH2PO4.2H2O, anhydrous sodium carbonate, 2.8% CaCl2, lactalbumin hydrolysate, HCl, NaOH and phenol red to a healthy ox heart-lung tissue with fat and tendons removed without adding 20% serum and calf thymus DNA. The special culture medium MB-HLS disclosed by the invention has the advantages of low cost and high mycoprotein content and provides powerful technical support for the research and large batch production of a mycoplasma bovis vaccine.

The invention relates to a freeze-dried vaccine of porcine transmissible gastroenteritis. The vaccine comprises a porcine transmissible gastroenteritis virus serving as an antigen and a protective agent, wherein the protective agent comprises the following substances in percent concentration by mass: 2.4-4% of NZ-amine, 0.3-0.5% of monopotassium glutamate, 16-20% of sucrose, 6-20% of lactalbumin hydrolysate, 1-1.6% of gelatin and the balance of water. A freeze-drying protective agent used in the vaccine disclosed by the invention is low in raw material cost and simple to operate and can be produced on a large scale. The titer of the vaccine prepared by adopting the protective agent is greater than or equal to 105.7TCID50 (Tissue Culture Infectious Dose 50) / dose (national standard) after the vaccine is preserved for 24 months at 2-8 DEG C, so that the storage life of the vaccine is effectively prolonged.

The invention discloses a milk-derived polypeptide protein beverage capable of relieving pressure and helping sleep. The milk-derived polypeptide protein beverage comprises the following main components: raw milk, hydrolyzed casein powder, casein phosphopeptides, whey protein hydrolysate, gamma-aminobutyric acid, coconut powder, passion flower concentrated juice, concentrated pear juice and asparagus powder. The invention further discloses a preparation method of the polypeptide protein beverage. The prepared polypeptide protein beverage has the functions of shortening the sleep incubation period, reducing sleep interference, reducing body disorder, improving the conscious sleep quality, prolonging the sleep time and improving the sleep efficiency, and achieves the effects of relieving pressure and improving sleep.

The invention discloses a special culture medium for cymbidium sinense. The special culture medium comprises, by weight, 70-80 parts of Heller culture media, 15-20 parts of ferric sulfate, 8-10 parts of disodium EDTA (ethylene diamine tetraacetic acid), 6-8 parts of glucose, 8-10 parts of lactalbumin hydrolysate, 8-12 parts of maize endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut juice, 3-5 parts of indoleacetic acid, 3-6 parts of indolebutyric acid, 2-5 parts of naphthalene acetic acid, 3-6 parts of naphthoxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 1-3 parts of isopentene aminopurine, 1-3 parts of gibberellin, 20-30 parts of agar, 5-15 parts of activated carbon and 40-50 parts of water. The special culture medium for the cymbidium sinense has the advantages that the special culture medium contains rich nutrients, is manufactured for characteristics of cymbidium sinense plants and has an excellent market prospect, and the survival rate of the cymbidium sinense plants can be effectively increased.

The invention discloses a lower-serum and efficient culture medium of mycoplasma ovipneumoniae. The lower-serum and efficient culture medium is prepared from the following components: MEM, sodium pyruvate, glucose, Hank's liquid, fresh yeast leaching liquid, L-glutamine, L-cysteine, lactalbumin hydrolysate, calf thymus DNA (Deoxyribonucleic Acid), insulin, transferrin, penicillin, horse serum, phenol red and de-ionized water; the invention provides a preparation method of the lower-serum and efficient culture medium. The lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, has the beneficial effects that the content of the serum is only 5 percent and is 1 / 4 of the content of the serum in a culture medium in the prior art; the titer of mycoplasma ovipneumoniae semi-finished-product bacterium liquid prepared by the method reaches 10<10>CCU / ml and is obviously higher than that of the culture medium in the prior art. According to the lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, the sensitive stress response to a goat body, caused by heterologous serum, is alleviated, and the biosafety is improved; the titer of bacterium liquid of living bacteria is also improved and the production cost is reduced.

The invention discloses a lactalbumin hydrolysate. The lactalbumin hydrolysate is concentrated solution obtained by performing heat treatment with lactalbumin, performing hydrolysis with alkaline protease and performing ultrafilteration according to cut-off molecular weight of 10,000. The prepared lactalbumin hydrolysate comprises 18 kinds of amino acids which account for over 70 percent. Animal experiments prove that the prepared lactalbumin hydrolysate can obviously reduce glucose tolerance of mice, stimulate insulin secretion, obviously increase insulin content in the mice, and obviously reduce random blood glucose in the mice. The prepared lactalbumin hydrolysate can be used for preparing the glucose-lowering medicament, can obtain obvious effects, and has a great application prospect and a bright market prospect.

The invention relates to the technical field of probiotic mediums, especially to a probiotics medium and a culture method and application thereof. When the probiotics medium is a liquid medium, each 1L of the medium contains 10-12 g of lactalbumin hydrolysate, 5-6 g of yeast powder, 10-12 g of proteose peptone, 10-12 g of glucose, 3-5 g of sodium chloride, 180-200 ml of tomato juice, 1-2 ml of microscale salting liquid, and the rest of distilled water. When the probiotics medium is a solid medium, the medium also comprises 18-20 g of agar. The invention has the following beneficial effects: carbon nitrogen ratio is complete; vitamins and trace elements are complete; by the use of the medium, all probiotics can grow well; cost of the medium is low; operation is convenient; the medium is suitable for mass production; and problems existing in the prior art can be solved economically and effectively.

The invention, belonging to the technical field of clinical medicine, particularly relates to a clinical nutrition emulsion and a preparation method thereof. The clinical nutrition emulsion disclosed herein has the advantages of safety, reliability, and good stability. The clinical nutrition emulsion disclosed herein comprises 200-300g of lactalbumin hydrolysate, 200-300g of refined white granulated sugar, 200-300g of maltodextrin, 80-100g of medium-chain triglyceride, 90-100g of peanut oil, 10-15g of compound emulsifier, 5-15g of composite minerals, 0.5-1.5g of multivitamin, 30-50g of compound stabilizer, and 1-3g of immuno-potentiating active material. The preparation method disclosed herein comprises the following steps: respectively dissolving the raw materials and auxiliary materials in oil phase and water phase, mixing after dissolving completely; adding a colloid mill to the mixed solution, then carrying out homogenization on the formed colloid; after the colloid is homogeneous, bottling, sealing and sterilizing, wherein the sterilization temperature is 100-150 DEG C, and the sterilization time is 10-25 min; and finally labeling, and inspecting to obtain finished products.

The invention discloses a special high-speed high-density integral cell culture medium for BHK21 cells, which is used for culturing the BHK21 cells and comprises European balanced salt, known chemical component lactalbumin hydrolysate and a DMEM (Dulbecco's Modified Eagle Medium) cell culture medium, wherein the components and the contents in the integral cell culture medium are all known. The invention also discloses a method for preparing the special high-speed high-density integral cell culture medium for the BHK21 cells, which comprises the following steps of: firstly, weighing and grouping all raw materials, and respectively and sequentially carrying out mixed milling on the raw materials; and secondly, sequentially carrying out mixed milling on the mixed and milled raw materials. The high-speed high-density integral cell culture medium has the advantages of stable components, longer quality guarantee period and simple preparation process, is easy to control the quality, is easy to storage and can be widely applied to cell culture.