The invention, belonging to the technical field of clinical medicine, particularly relates to a clinical nutrition emulsion and a preparation method thereof. The clinical nutrition emulsion disclosed herein has the advantages of safety, reliability, and good stability. The clinical nutrition emulsion disclosed herein comprises 200-300g of lactalbumin hydrolysate, 200-300g of refined white granulated sugar, 200-300g of maltodextrin, 80-100g of medium-chain triglyceride, 90-100g of peanut oil, 10-15g of compound emulsifier, 5-15g of composite minerals, 0.5-1.5g of multivitamin, 30-50g of compound stabilizer, and 1-3g of immuno-potentiating active material. The preparation method disclosed herein comprises the following steps: respectively dissolving the raw materials and auxiliary materials in oil phase and water phase, mixing after dissolving completely; adding a colloid mill to the mixed solution, then carrying out homogenization on the formed colloid; after the colloid is homogeneous, bottling, sealing and sterilizing, wherein the sterilization temperature is 100-150 DEG C, and the sterilization time is 10-25 min; and finally labeling, and inspecting to obtain finished products.