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Probiotics medium and culture method and application thereof

A technology of culture medium and beneficial bacteria, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of high cost, difficult to store, complicated operation, etc., and achieve the effects of convenient operation, inhibiting the growth of pathogenic bacteria, and low cost.

Inactive Publication Date: 2017-07-07
北京中特养生物技术研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But for microorganisms, each strain has its own growth medium suitable for growth. In large-scale production, although the compound bacteria preparation of multiple bacteria will work well, each dedicated culture medium for each strain will cause confusion and confusion. Troublesome, high cost, and complicated operation; and some special media need to use sheep blood, amniotic fluid, cysteine ​​and other expensive and difficult-to-preserve medicines, which are also not conducive to industrial production and use

Method used

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  • Probiotics medium and culture method and application thereof
  • Probiotics medium and culture method and application thereof

Examples

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Embodiment 1

[0033] A kind of beneficial bacteria culture medium, described beneficial bacteria culture medium is liquid culture medium, comprises hydrolyzed milk protein 10g, yeast powder 5g, Peptone 10g, glucose 10g, sodium chloride 5g, tomato juice 200ml, trace salt solution 1ml, the rest is distilled water;

[0034] As described in this embodiment, every 1 L of the trace salt solution includes 5 g of magnesium sulfate, 0.5 g of manganese chloride, and 0.5 g of ferric chloride.

[0035] As described in this example, the pH of the culture medium for beneficial bacteria is controlled at 7.2.

[0036] As described in this embodiment, sodium hydroxide solution and dilute hydrochloric acid solution are used to adjust the pH of the beneficial bacteria culture medium.

[0037] As described in this embodiment, the concentration of sodium hydroxide solution is 2mol / L.

[0038] As described in this embodiment, the mass concentration of the dilute hydrochloric acid solution is 10%.

Embodiment 2

[0040] This embodiment is improved on the basis of Embodiment 1, and the content described in Embodiment 1 is also included in this embodiment, and will not be described in detail here.

[0041] A kind of beneficial bacteria culture medium, described beneficial bacteria culture medium is liquid culture medium, comprises hydrolyzed milk protein 11.7g, yeast powder 5.3g, every 1L Peptone 10.2g, glucose 12g, sodium chloride 3.6g, tomato juice 190ml, trace salt solution 1.2ml, the rest is distilled water;

[0042] As described in this example, the pH of the beneficial bacteria culture medium is controlled at 7.6.

Embodiment 3

[0044] This embodiment is improved on the basis of Embodiment 1, and the content described in Embodiment 1 is also included in this embodiment, and will not be described in detail here.

[0045] A kind of beneficial bacteria culture medium, described beneficial bacteria culture medium is a liquid culture medium, comprises hydrolyzed milk protein 10.4g, yeast powder 6g, Peptone 10.8g, glucose 11.3g, sodium chloride 4.4g, tomato juice 185ml, trace salt solution 1.5ml, the rest is distilled water;

[0046] As described in this example, the pH of the culture medium for beneficial bacteria is controlled at 7.4.

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Abstract

The invention relates to the technical field of probiotic mediums, especially to a probiotics medium and a culture method and application thereof. When the probiotics medium is a liquid medium, each 1L of the medium contains 10-12 g of lactalbumin hydrolysate, 5-6 g of yeast powder, 10-12 g of proteose peptone, 10-12 g of glucose, 3-5 g of sodium chloride, 180-200 ml of tomato juice, 1-2 ml of microscale salting liquid, and the rest of distilled water. When the probiotics medium is a solid medium, the medium also comprises 18-20 g of agar. The invention has the following beneficial effects: carbon nitrogen ratio is complete; vitamins and trace elements are complete; by the use of the medium, all probiotics can grow well; cost of the medium is low; operation is convenient; the medium is suitable for mass production; and problems existing in the prior art can be solved economically and effectively.

Description

technical field [0001] The invention relates to the technical field of beneficial bacteria culture medium, in particular to the beneficial bacteria culture medium and its cultivation method and application. Background technique [0002] In today's world, human beings have realized that the imbalance of micro-ecology can bring about the danger of disease, and have recognized the potential harm to the human body caused by antibiotics, hormones, chemicals and pesticide residues in food. In order to prevent diseases, improve human health, reduce environmental pollution and competitive pressure, human beings need to return to nature and call for green food and medicine. Probiotics and antimicrobial peptide products are emerging in fields such as health care, medicine, food, cosmetics, feed, and environmental protection. More and more countries oppose the use of antibiotics as growth promoters and pass legislation to ban them. Alternatives to antibiotics have become a hot topic ...

Claims

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Application Information

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IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 谭瑛
Owner 北京中特养生物技术研究所有限公司
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