The invention relates to the field of flower propagation methods, in particular to the field of
corm flower propagation methods. A
thin layer culture method of tulips comprises the following steps of: longitudinally or transversely
cutting tulip scales into thin
layers of 0.1-5mm, and laying the thin
layers on a culture medium; and inducing and proliferating the culture medium into an MS (Murashige and Skoog) culture medium, wherein 1.0-2.0mg of 6-BA (Benzyl
Aminopurine), 0.1-1.0mg of NAA (Naphthyl
Acetic Acid), 1.0-4.0mg of 2, 4-D (2,4-
Dichlorophenoxyacetic acid), 20g of
cane sugar and 6g of agaragar are added to the MS culture medium per L, the culture condition is that the temperature is 23+ / - 2 DEG C, the illumination intensity is 1,500-2,000Lx, and the illumination time is for 16h / d. The
thin layer culture method of the tulips has the advantages and active effects: the
tissue culture bulb inductivity reaches 100%, induced
tissue culture seedlings grow vigorously, the source of the
tissue culture seedlings is not limited by seasons, and the tissue culture scales are the better source of
thin layer culture materials.
Cutting modes cause different inductions on bulbs, and the differentiation rate and the
bulb induction number of the scales which are transversely
cut are higher than those of the scales which are longitudinally
cut.