160 results about "Anoectochilus" patented technology

The invention relates to an undergrowth cultivation medium and cultivation method for anoectochilus formosanus. The undergrowth cultivation method for the anoectochilus formosanus comprises the steps of (1) selecting a forest environment with a natural shading degree of 60-90% and illumination intensity of 2000-5000lux to serve as a cultivation place of the anoectochilus formosanus; (2) forming shallow holes in the cultivation substrate in a scratched mode, inserting the root of each anoectochilus formosanus seedling into each shallow hole, covering each root, and watering rooting water; (3) adjusting air humidity to be 75-90%, adjusting the temperature to be 15-30DEG C; (4) forming the cultivation medium, wherein the cultivation medium mainly comprises 8-23 parts of wood chip smashed objects, 8-25 parts of differentiated yellow loam and 65-80 parts of under-forest humus soil in a mixing mode; and (5) carrying out spraying watering when the surface of the cultivation medium whites after planting, and stopping watering when the cultivation medium is thoroughly watered every time. According to the undergrowth cultivation medium and cultivation method for the anoectochilus formosanus, due to the facts that the forest environment which is in natural growth and has the appropriate shading effect serves as the cultivation base, and the high-quality and high-efficiency cultivation medium is selected, growth of the anoectochilus formosanus is facilitated, the yield, the survival rate and the drying rate of the anoectochilus formosanus can be remarkably improved, operation is convenient, and production cost is low.

The invention relates to an aqueous culture method of anoectochilus. In the method, anoectochilus germchits are cleaned and sterilized, transplanted in a culture dish and cultured in a culture groove filled with an aqueous culture solution; each 10L of aqueous culture solution contains the following components: 650 to 800mg of calcium nitrate tetrahydrate, 50 to 60mg of ammonium nitrate, 280 to 350mg of potassium nitrate, 100 to 130mg of potassium dihydrogen phosphate, 350 to 380mg of magnesium sulfate, 0.5 to 0.8mg of potassium iodide, 2 to 5mg of manganese sulfate, 0.2 to 0.3mg of sodium molybdate, 0.1 to 0.2mg of cobalt chloride, 2 to 4mg of boric acid, 0.5 to 1mg of zinc sulfate, 0.3 to 0.5mg of copper sulfate and 3 to 5ml of iron salt solution, wherein the iron salt solution is prepared by mixing water, green vitriol and ethylenediamine tetraacetic acid disodium. The method has the advantages of lowering production cost, simplifying production process, improving the growth of anoectochilus plants and improving the survival rate of anoectochilus plants.

The invention provides a water culture technique for anoectochilus formosanus and relates to a pollution-free water (fog) culture production technique for Chinese medicament anoectochilus formosanus. The water culture technique adopts plastic disks and water grooves as culture containers and comprises the following steps of: transplanting the seedlings which are prepared by tissue culture and subjected to cleaning and sterilizing into the plastic disks; wherein the plastic disks are placed in the water grooves with nutrient solution; and performing culture management by fertilizing, changing the nutrient solution, atomizing, lighting, controlling humidity, controlling temperature and changing air. The water culture technique has the advantages that: the survival rate of transplanting the noectochilus formosanus seedlings prepared by tissue culture can be improved to over 98 percent; the fresh weight of the finished products in each square meter area is over 800 g; the production process is simple; the yield is high; the occupied area is small; and the water culture technique is suitable for culture by intensive and pollution-free facilities or scattered culture by farmers.

The invention discloses a greenhouse cultivation method for anoectochilus formosanus. The greenhouse cultivation method comprises the four steps of acclimating anoectochilus formosanus seedlings, disinfecting a cultivation substrate, planting, and controlling temperature and light, wherein in the step of planting, a cultivation cup after the anoectochilus formosanus seedlings are planted is placed into a plastic bag, and a plurality of small holes are formed in the position where the plastic bag higher than the cultivation cup; and water is poured into the bottom of the plastic bag, a bag opening in the plastic bag is sealed, and the plastic bag is hung below a seedling bed in a greenhouse. Due to the adoption of the technical scheme, the water is evaporated in the bag, so that the water is not liable to run away and a wetting environment suitable for growth of the anoectochilus formosanus is formed in the plastic bag. The anoectochilus formosanus seedlings are spaced from the outside environment through the plastic bag, the water is not liable to run away and the anoectochilus formosanus seedlings do not need to be watered in the later period after being planted, so that the anoectochilus formosanus seedlings cannot be polluted by various bacteria in air or water and the pest and disease damage is avoided. Therefore, higher survival rate of the anoectochilus formosanus seedlings can be also guaranteed in the absence of farm chemicals.

The present invention relates to Roxburgh anoectochilus cultivating apparatus and technology. The Roxburgh anoectochilus cultivating apparatus includes cultivating house, seedling tray shelves and seedling trays arranged orderly inside the cultivating house. The cultivating house may be intelligent or shaded net shanty; and the seedling tray shelves may be one or several layer structure in the size fitting the standard seedling trays. The tissue cultivated seedling is washed, disinfected and planted in the medium inside the seedling tray of grey moss with seedling survival rate over 98%. The cultivating technology includes reasonably controlling light, water spraying, fertilizer applying, humidity, etc. With five layer seedling tray shelves, fresh Roxburgh anoectochilus over 2.2 kg / sq m may be harvested. The present invention has the features of low cost, high yield, etc.

The invention provides a key technology of an organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation, belonging to a massive propagation and cultivation technology for the roxburgh anoectochilus terminal bud. According to the key technology, a terminal bud and a stem of the roxburgh anoectochilus terminal bud are taken as the material, in industrialization, the organic additive is adjusted and added timely, and the entire plant grows up after induction cultivation, enrichment cultivation and root induction, and the rooting percentage can reach 99%; and after hardening-seedling, the plant can be transplanted into humus, and the rate of survival can reach above 99%. According to the technology, the time for enrichment cultivation is short, the expanding propagation coefficient is high, the seedling can grow up once and is healthy, the whole roxburgh anoectochilus terminal bud plant is thick, the stem is thick and the roots are flourishing, the color of leaves is dark, the leaves have obvious fine hair, the leaf vein gold threads are clear, the rate of survival is high, the weight of each plant is 4.5-5.5g, and the economic efficiency is obvious.

The invention provides an interplanting cultivation device for anoectochilus formosanus and dendrobium officinale. The interplanting cultivation device comprises a thin film greenhouse and an interplanting shelf placed in the thin film greenhouse. The interplanting shelf is a three-dimensional triangular shelf formed by two longitudinal isosceles triangle frames and three transverse parallel rectangular frames. Dendrobium officinale planting frames formed by mutually stacking trunks and / or bamboo rods are placed on an upper layer and a middle layer. An anoectochilus formosanus planting tray shelf is arranged on a lower layer. The dendrobium officinale is planted into gaps among the upper and lower trunks of the upper layer and the middle layer of the interplanting shelf. The anoectochilus formosanus is planted in planting trays, with matrixes, on the lower layer of the shelf. The relative humidity in the thin film greenhouse is controlled to be 70-90%, the temperature is controlled to be 18-25 DEG C, the light transmittance is 60-80%, and spraying and ventilating facilities are arranged. Roots of the dendrobium officinale adhere to the trunks so as to shade the anoectochilus formosanus on the lower layer, the anoectochilus formosanus on the lower layer is cultivated in a lifting mode by adopting the matrixes, the water permeability is good, soil insect harm can be avoided, organic fertilizer sprayed from the upper layer can be fully utilized, the planting cost is lower, the harvesting reasons are spaced, and benefits are higher.

The invention discloses a method for regenerating and planting medicinal anoectochilus roxburghii with the stem removed but the root left, which has the key point of regeneration with the root left and stem removed. First, strong tissue culture seedling are elected and then transmitted to be planted in a mixed substrate consisting of soil, coarse sand and wood dust after plantlet hardening and watered with a mixed liquid of root agent and carbendazim wettable powder. Field management, fertilization and disease preventing and treating are required to be respected during the growth period of the anoectochilus roxburghii. By applying the method of regeneration with the stem removed and the stem left, the anoectochilus roxburghii stems on the ground are harvested but a stem node at each stem is left before the bolting and blossom of the anoectochilus roxburghii. After harvesting, the remained plant is sprayed with a mixed liquid of 6-benzyl aminopurine and carbendazim wettable powder. The method aims at solving the problem of narrow availability of the anoectochilus roxburghii seedling and easily sprouting of the stem node thereof and adopts the production method of regeneration with the stem removed and the stem left, thereby improving the seedling use ratio, guaranteeing the product supply and realizing continuous production. Meanwhile, the complete retaining of the root system creates the good condition for the quick growth of new sprouts and therefore, the regeneration method of the invention has comparatively high production efficiency and good economic benefit.

The invention discloses a method for culturing a test tube plantlet of Xingren Anoectochilus, which comprises the steps of: selecting a stem section with an axillary bud as an explant, carrying out collateral bud induction and enrichment culture, grafting the collateral bud into a strong seedling rooting culture medium for strong seedling rooting cultivation, and obtaining the test tube plantlet, wherein the culture medium used for collateral bud induction and enrichment culture is MS+(2-5)mg / l 6-BA (butyl acrylate)+(0.2-0.5)mg / l NAA (naphthyl acetic acid)+8g / l agar+30g / l sugar pH: 5.7-6.0; and the strong seedling rooting medium is (1 / 2MS-MS)+(0.01-0.05)mg / l 6-BA+(0.05-0.10)mg / l NAA+8g / l agar+(15-25)g / l sugar pH: 5.7-6.0. The Xingren Anoectochilus has the propagation coefficient of more than 10 times, so that the test tube plantlet is thick and strong and has the transplant survival rate of more than 95%.

The invention discloses a hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds. According to the hormone-free rapid tissue culture and propagation method, no hormone is used in the whole process, the mature and non-cracking fruits, which is pollinated for 5 months after flowering, of wild or artificially planted roxburgh anoectochilus terminal bud distributed in Szechuan Emei region are taken as the material, and seedlings are bred efficiently and quickly according to the route from seeds to protocorms, complete plants and acclimatization and transplant. A hormone-free culture medium is adopted in the whole process; more than 5000 good-quality seedlings can be quickly bred from one mature fruit by virtue of tissue culture of about 10-12 months, and after acclimatization and transplant, the greenhouse culture survival rate can be above 95%. According to The hormone-free rapid tissue culture and propagation method of the Emei roxburgh anoectochilus terminal bud seeds is capable of keeping the good traits of the Emei roxburgh anoectochilus terminal bud and maintaining the good medicinal properties of the Emei roxburgh anoectochilus terminal bud; besides, the method is simple, and the cost is low; industrialized seedling production can be realized; no hormone is adopted so that the safety of the seedlings can be ensured; the hormone-free rapid tissue culture and propagation method has important significance on regeneration and sustainable utilization of critically endangered valuable and rare resources of medicinal plants and protection of seed resources.

The invention discloses a hydroponic growing method of anoectochilus roburghii. The hydroponic growing method comprises the steps of washing anoectochilus roburghii tissue culture bottle seedlings; transferring to a hydroponic device for culturing; placing the anoectochilus roburghii tissue culture seedlings into a culture bed on the hydroponic device; and only soaking roots into a culture solution, wherein hydroponic growing is divided into two stages: in the first stage, the anoectochilus roburghii is darkly cultured for 15 days at the room temperature of 27 + / -2 DEG C and the humidity of 80%-90%; in the second stage: the anoectochilus roburghii is brightly cultured for 75 days at the room temperature of 27 + / -2 DEG C and the humidity of 80%-90%, the illumination of the anoectochilus roburghii lasts for 9 hours per day and the illumination intensity is 1,700-2,000 Lux. By using the hydroponic growing method, the survival rate can be increased, and the culturing time can be shortened so as to realize industrial planting.

The invention discloses a tissue culture rapid propagation method of anoectochilus formosanus, and belongs to the technical field of plant tissue culture. By adopting the tissue culture rapid propagation method, the survival rate of transplanting and the growth coefficient can be increased, and the good characters and the good drug properties of the Emei anoectochilus formosanus can be kept. The tissue culture rapid propagation method comprises the following steps: selecting wild Emei anoectochilus formosanus growing in May to October, preparing an explant, carrying out disinfection treatment, inducing cluster buds, carrying out secondary culture and rooting and strengthening culture, and transplanting to greenhouses for culturing, and rapidly propagating the anoectochilus formosanus through hormone induction. The tissue culture rapid propagation method can be used for ensuring the good characters and the good drug properties of the Emei anoectochilus formosanus, is simple and low in cost and can realize industrialized seedling production. The percentage of inducing the cluster buds and rooting by adopting the method reaches above 90%, the survival rate of transplanting is above 95%, and the growth coefficients within four months can reach 8-10 times. The Emei anoectochilus formosanus produced by adopting the method does not mutate, is high in yield, low in cost and short in period and has relatively strong market competitiveness.

The invention discloses nutritional rice with unique rose fragrance and a preparation method thereof. The nutritional rice consists of raw materials such as polished round-grained rice, small glutinous millets, chestnut kernels, roses, eucheuma, mangosteen, gelidium amansii, sorghum rice, water chestnuts, pitaya, pigskin, broccoli, peach gum, whole egg powder, onion powder, dried orange peel, immature bitter orange, roxburgh anoectochilus terminal bud and the like. The nutritional rice has beneficial effects that the rice is rich in nutritional substances such as dietary fibers, the digestion is promoted, the blood fat can be reduced, and the health of a human body is favored; the rice is soft and glutinous in taste, unique in taste, suitable for various people to eat and capable of increasing the appetite, improving the anti-disease capacity and enhancing the body.

The invention provides a preparation method of a roxburgh anoectochilus terminal bud extracts containing kinsenoside and application of the extracts. The roxburgh anoectochilus terminal bud extracts are is prepared from roxburgh anoectochilus terminal bud extraction liquid, roxburgh anoectochilus terminal bud thick paste, roxburgh anoectochilus terminal bud dry paste and roxburgh anoectochilus terminal bud dry paste powder. The preparation method comprises the work procedures of medical material alcohol extraction, water extraction, reduced pressure concentration, drying, crushing and the like. The method has the advantages that the transfer rate of the effective ingredient of the kinsenoside can be improved by 50 percent or higher through being comparedin comparison with a conventional water extraction process; the utilization rate of the rare medicinal materials is obviously improved; the process operability is high; the repeated performance is good; the equipment requirement is low;the preparation method is applicable to industrialized production. The roxburgh anoectochilus terminal bud extracts prepared by the method can be used for preparing health care food; the taking and the carrying of the finished product are is convenient to take and carry; the quality is stable; the auxiliary protection effect is achieved on the chemical liver damage; the effects of assistance in reducing the blood fat in an auxiliary way and reducing the blood sugar in an auxiliary way areis obvious.

The invention discloses a planting method for planting a tissue culture seedling of anoectochilus roxburghi in a greenhouse. The planting method for planting the tissue culture seedling of the anoectochilus roxburghi in the greenhouse comprises the following steps of (1), selecting a field; (2), constructing the greenhouse; (3), constructing a mobile seedling bed; (4), constructing a seedling culturing tray; (5), selecting a substrate; (6), treating the tissue culture seedling; (7), managing the plantation; (8), managing the harvest. The planting method is high in growing survival rate which can be up to 95 percent or above, short in growth cycle which only needs 4 to 5 months from the plantation to the harvest, and high in land utilization rate, is not limited by the quality of the soil, provides technical services for the later-stage seedling exercising planting of the plant tissue culture of the anoectochilus roxburghi, is simple to operate, low in investment, low in cost and suitable for large-scale industrialized production.

The invention provides a gynura bicolor heat-clearing buccal tablet and a preparation method thereof. The buccal tablet is prepared from the following main raw materials in parts by weight: 40-50 parts of gynura bicolor dried powder, 5-10 parts of roxburgh anoectochilus terminal bud dried powder, 5-10 parts of lonicera japonica dried powder, 5-10 parts of licorice root dried powder, 1-5 parts of konjac glucomannan, 1-5 parts of corn starch and 1 part of xylitol. The preparation method comprises the following steps: selecting materials, washing, preparing dried powder of raw materials, tabletting, coating, sterilizing and packaging. According to the gynura bicolor heat-clearing buccal tablet and the preparation method thereof, the raw material utilization can be improved, nutrients in gynura bicolor and other raw materials can be effectively maintained, absorption of human bodies for the nutritents can be improved, and the buccal tablet is a purely natural herbal health buccal tablet with health and safety. The method is simple in process, strong in operability and high in production efficiency, and is suitable for large-scale production to enrich the deep processing mode of gynura bicolor industry.

The invention discloses a low temperature preservation method for roxburgh anoectochilus terminal bud germplasm resources. The low temperature preservation method comprises the following steps: collecting wild seedlings, carrying out primary treatment and high temperature passivation on explants; carrying out germicidal treatment on a clean bench, and then transferring into a roxburgh anoectochilus terminal bud initial medium for low temperature induced differentiation; carrying out low-temperature dark treatment for a period of time after the differentiation, and then transferring bud balls into a roxburgh anoectochilus terminal bud subculture medium; cultivating the bud balls for a period of time under the condition of normal-temperature radiation, then transferring the bud balls for low-temperature dark treatment, and finally transferring plantlets into a roxburgh anoectochilus terminal bud medium for cultivation under normal-temperature radiation. The method disclosed by the invention has the advantages of low cost, simple operation and high practicability, and can protect the precious medicinal resource of roxburgh anoectochilus terminal bud well, so that the biological diversity is protected.

The invention discloses a method for planting anoectochilus roburghii. Multiple small holes are punched in the side face of a container at intervals. Soil is filled into the container. Seedlings of the anoectochilus roburghii are planted into the small holes, and after water is sprayed to perform rejuvenation on the seedlings, the container where the acoectochilus roburghii is planted is hung on branches below woods or on a shelf below the woods. Due to the fact that the original mode that the anoectochilus roburghii grows on the ground in the natural environment is modified as the mode that the anoectochilus roburghii is planted below the woods, invasion of wild animals such as insects, batrachia and snakes can be avoided, it can be avoided that root systems of the anoectochilus roburghii are exposed and dead due to rain wash, the survival rate and the yield of the anoectochilus roburghii are improved, and medicine property components of the anoectochilus roburghii are increased.

The invention discloses an underwood planting method of anoectochilus formosanus. The method is characterized by including the steps that first, forest land is prepared, wherein, a plurality of water spraying pipes are arranged above a forest site, an underwood soil layer is ploughed so as to loosen and break the soil layer, and a dead leaf layer with the thickness ranging from 2 cm to 3 cm is laid above the ploughed soil layer; second, anoectochilus formosanus seedlings are planted, wherein the anoectochilus formosanus seedlings are obtained through tissue culture and planted into the ploughed soil layer; third, underwood management is performed, wherein in the growth stage of the anoectochilus formosanus seedlings, when the dead leaf layer is in a dried state, the water spraying pipes are adopted for spraying water, and therefore the dead leaf layer can be kept moist. According to the method, dead leaves are stimulated to decompose with a fermentation method to generate small molecule nutrient substances capable of being easily absorbed by anoectochilus formosanus, meanwhile, dead leave powder can provide a good moist environment for anoectochilus formosanus, and therefore the fresh weight of the anoectochilus formosanus and the content of medical composition of the anoectochilus formosanus are increased.

The invention relates to a seedling hardening and transplant acclimatization method for roxburgh anoectochilus terminal bud tissue culture seedlings and aims to obviously increase the transplant survival rate of the roxburgh anoectochilus terminal bud tissue culture seedlings. The method has the characteristics of high economical efficiency, practicability, high simpleness in operation, high easiness in mastering, high standardability, high efficiency and the like. According to the technical scheme, the method comprises the following steps: firstly, selecting a seedling hardening field; secondly, constructing a seedling hardening greenhouse; thirdly, selecting tissue culture bottle seedlings; fourthly, selecting seedling hardening matrix; fifthly, management before the bottle seedlings are taken out of bottles; sixthly, washing the seedlings out of the bottles; seventhly, transplanting and management.

The invention relates to the field of plant cultivating, in particular to a method for cultivating anoectochilus formosanus in a wild imitation mode. The method comprises the following steps that (1) seedling exercising is carried out on anoectochilus formosanus tissue cultured seedlings in a pre-cultivating field, and the pre-cultivating field is an earth surface humus horizon under an original ecological broad leaved forest; (2) the excised anoectochilus formosanus tissue cultured seedlings are taken out of tissue culturing bottles, and culturing media are washed away through running water; (3) the tissue cultured seedlings washed in the step (2) are steeped with a carbendazim solution for ten minutes; (4) anoectochilus formosanus cultivating soil is disinfected before the anoectochilus formosanus is cultivated, root systems of the anoectochilus formosanus in the step (3) are straightened and buried in the disinfected soil, and root fixing water is applied; (5) cultivating management is carried out on the anoectochilus formosanus until harvesting is conducted. According to the method, the anoectochilus formosanus is cultivated in the earth surface humus horizon of the original ecological broad leaved forest, the earth surface humus horizon of the original ecological broad leaved forest originally has a large amount of distributed wild anoectochilus formosanus and is quite suitable for cultivating the anoectochilus formosanus in the wild imitation mode, and the survival rate of the anoectochilus formosanus cultivated artificially is improved.

The invention provides a method for promoting germination and seedling formation of anoectochilus formosanus seeds. According to the technical scheme, an idea that the anoectochilus formosanus seeds can germinate only through symbiosis with fungi is found by virtue of an experimental measure, a set of fungus acquiring methods and symbiosis culture conditions are explored under the guidance of the idea, meanwhile, a strain capable of outstandingly promoting the germination and seedling formation of the anoectochilus formosanus seeds on a sawdust matrix is definitized. Particularly, the method comprises the steps of separating endophytic fungi from the root of wide anoectochilus formosanus, mixing a mixed fungal colony or a purified specific colony with the disinfected anoectochilus formosanus seeds in the sterile sawdust matrix, culturing for one week under a dark condition, and then culturing at specific illumination intensity and temperature for specific illumination time until the seeds germinate. According to the technical scheme, the germination velocity of the anoectochilus formosanus seeds is increased, meanwhile, the method has good repeatability due to a clear principle.

The invention discloses a method for preparing anoectochilus formosanus vinegar through liquid fermentation. The method comprises the following steps of (1) preparing a fermented vinegar solution by carrying out liquid fermentation on sugarcane processing residues serving as raw materials, namely cleaning the sugarcane processing residues, smashing, then, mixing the sugarcane processing residues and water, adding a compound enzyme into the mixture to carry out liquification fermentation, and then, adding a saccharifying enzyme to carry out saccharification fermentation; next, adding an active dry yeast to carry out alcohol fermentation; and finally, adding activated acetic bacteria to carry out acetic fermentation, filtering and collecting filtrate; (2) preparing dry powder of anoectochilus formosanus, namely cleaning annual anoectochilus formosanus plants, drying, and smashing for later use; and (3) preparing the anoectochilus formosanus vinegar, namely mixing the fermented vinegar solution prepared by carrying out liquid fermentation on the sugarcane processing residues serving as the raw materials and the dry powder of the anoectochilus formosanus, uniformly stirring the mixture, processing at high temperature, and airing at normal temperature to obtain the anoectochilus formosanus vinegar. The anoectochilus formosanus vinegar prepared by using the method is soft in sour, mellow in taste, faint scent and elegant and is also rich in the anoectochilus formosanus and beneficial components in sugarcane bagasse and leaves. The method is easy to master and capable of realizing large-scale production, waste utilization and environment protection.

The invention relates to a production method of anoectochilus symbiosis seedling, which comprises the following steps that: (1) endophytic fungi culturing: after being activated from a slant tube specie preserved at low temperature, rhizoctonia sp. JSH-1 with the preservation number of CGMCC No. 3074 which is separated from the root of wild anoectochilus is transferred into a PDA plate and cultured for 2 to 4 days in dark at 28DEG C; then a mycelial agar block is prepared; and (2) anoectochilus tissue culture symbiosis: the top leaf bud of the sterile anoectochilus tissue culture seedling are removed, sterile operation is adopted to cut off the leaf bud from the middle parts of the two eustipes to obtain 2 to 4 stems, and then the stems are simultaneously grafted to a symbiotic culture medium to be symbiotically cultured for 2 to 3 months together with the rhizoctonia sp. JSH-1 mycelial agar block. The stem symbiotic anoectochilus tissue culture symbiosis culture method can greatly reduce the use cost of anoectochilus tissue culture seedling, shorten the growth cycle, improve the survival rate so as to reduce the production cost and facilitate the realization of industrial production.

The invention provides a formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings for one step. Each liter of a nutrient solution comprises the following components: 1-3g of hyponex I, 1-2g of hyponex II, 0.5-1g of hyponex V, 0.1g of inositol, 2g of peptone, 1.6g of active carbon, 7g of agar, 20g of sugar, 62.5g of banana, 0.5g of potassium nitrate, 0.5g of ammonium nitrate, 0.25mg of indolebutyric acid, 0.25-0.5mg of naphthylacetic acid and 0.25mg of rooting powder; the pH value of the nutrient solution is adjusted to 5.6. The formula is convenient to prepare; the transferring steps are reduced; the pharmaceutical costs and the artificial costs are reduced; the pollution rate is reduced; the rate of seedling emergency is high; the practical effect is good; the tissues begin to sprout in 2 months after inoculating and begin to strike root in 3 months, and the tissues are cultured to normal tissue culture seedlings after 4 months; the rate of seedling emergency is greater than 96%; each tissue culture seedling is relatively strong; after transplanting the tissue culture seedlings for 2 months, the counted survival rate reaches more than 95%, so that the production period of the roxburgh anoectochilus terminal bud seedlings is shortened, thus facilitating quick breeding of tissue culture seedlings in factories.

The invention discloses a making method of roxburgh anoectochilus terminal bud health-care instant tea powder. The roxburgh anoectochilus terminal bud health-care instant tea powder is made by taking roxburgh anoectochilus terminal buds as the main raw material through the steps of raw material preparation, tea powder extracting solution preparation, enzymolysis, crushing, mixing, homogenizing, freeze drying, finishing and packaging and the like. According to the method, the roxburgh anoectochilus terminal buds are subjected to steam fixation, the astringent taste in the raw material is removed, the raw material is subjected to beating through a tea powder extracting solution, and nutrient substances are better enriched; by means of composite enzyme treatment, the nutrient substances of the roxburgh anoectochilus terminal buds are fully preserved, and the use ratio of the raw material is increased; by means of homogenizing, the mouthfeel of the finished tea powder product is improved, the quality of the finished tea powder product is improved, and therefore the finished instant tea powder product has the effects of clearing heat, cooling the blood, balancing yin and yang, promoting secretion of saliva or body fluid, beautifying skin, prolonging life and the like.

The invention discloses a multidirectional disturbance type device for preparation of roxburgh anoectochilus terminal bud, which comprises a drying shell body; the drying shell body closing to left and right side walls is provided with a left installing block and a right installing block; the upsides of the left installing block and the right installing block are fixedly connected to a flexible device; the flexible device comprises a supporting pillar which is arranged vertically, and the supporting pillar crosses through the drying shell body; a groove with a downwards opening is arranged onthe supporting pillar, and the inner top part of the groove is fixedly connected with a spring; the lower end of the spring is fixedly connected with a rack, one side of the rack is meshed with a gear; the gear is fixedly connected with a rotary shaft, and the rotary shaft is erected on the supporting pillar; one end of the rotary shaft is fixedly connected to a motor output shaft, outsides of theleft installing block and the right installing block are provided with a left slide slot and a right slide slot; a connecting rod is arranged in each of the left slide slot and the right slide slot.The multidirectional disturbance type device is reasonable in structure design, convenient to use and high in drying efficiency; besides, the multidirectional disturbance type device is valuable to popularize and apply.

A roxburgh anoectochilus terminal bud-dendrobium officinale soft capsule is disclosed. The soft capsule comprises roxburgh anoectochilus terminal bud and dendrobium officinale which are mixed according to a weight ratio of 1-1.5:2-3. The soft capsule is prepared by following steps of: mixing the roxburgh anoectochilus terminal bud and the dendrobium officinale according to the ratio, drying until the weight is constant, smashing to obtain composite medicine powder, adding distilled water into the composite medicine powder, dipping, putting into a thermostat water bath, filtering, keeping an extracting solution, adding distilled water with the same amount, repeating the above steps, combining the extracting solutions, filtering with gauze to remove impurities, centrifuging at a high speed, subjecting the liquid supernatant to rotary evaporation and concentration to obtain a concentrate liquid, adding absolute ethyl alcohol into the concentrate liquid to obtain a concentrate solution, subjecting the concentrate solution to alcohol precipitation, performing centrifugal separation to separate the ethyl alcohol and a precipitate, performing alcohol precipitation to obtain the precipitate, adding ethyl alcohol and acetone into the precipitate to wash the precipitate, subjecting the precipitate to evaporation in a boiling water bath, putting the precipitate into a drying oven, drying until the weight is constant to obtain a roxburgh anoectochilus terminal bud-dendrobium officinale polysaccharide composite, and preparing the soft capsule. The soft capsule is enhanced in medicine effects, saved in medicine materials, accurate in dosage and convenient to eat.

The invention discloses a preparation process of roxburgh anoectochilus terminal bud concentrated solution, which comprises the following steps of carrying out a set of processes including extracting, cooling, centrifuging, ultrafiltering, reverse osmosis concentration, sterilizing and filling on roxburgh anoectochilus terminal bud as a raw material, thereby obtaining roxburgh anoectochilus terminal bud concentrated solution. According to the invention, a water extraction method and a membrane separation technology are adopted; effective components and natural colour, aroma and taste of roxburgh anoectochilus terminal bud are reserved to a great extent; the quality of roxburgh anoectochilus terminal bud concentrated solution is ensured; the prepared roxburgh anoectochilus terminal bud concentrated solution is orange red and bright in colour, mellow in taste and pure in aroma; the preparation process is scientific and reasonable; the flow is easy to control, and therefore, the preparation process disclosed by the invention is applied to industrial production and is beneficial to expanding deep processing utilization of roxburgh anoectochilus terminal bud; scientific basis is provided for further developing roxburgh anoectochilus terminal bud series of dietary supplements; the industrial value of roxburgh anoectochilus terminal bud is greatly increased; essence of roxburgh anoectochilus terminal bud is scientifically extracted by adopting the advanced process, and therefore, the effective components and the special flavour of roxburgh anoectochilus terminal bud are reserved to a great extent.