The invention discloses thermosetting type glass screen printing ink. The thermosetting type glass screen printing ink includes, by weight, 40%-65% of Evonik EP112 saturated polyester, 21%-45% of titanium dioxide TR-92, 0.5%-2.0% of fumed silica A-200, 10%-15% of methylated amino resin MF915, 1.0%-4.0% of adhesion promoters LTW, 0.5%-1.5% of antifoaming agents FOAMEXN, 0-1.0% of flatting agents BYK-310, 0-1.0% of brightening agents OB and 2%-6% of DBE solutions. A preparation method of the thermosetting type glass screen printing ink includes the steps that the saturated polyester, the titanium dioxide and the fumed silica are firstly stirred evenly, then the methylated amino resin, the adhesion promoters, the antifoaming agents, and / or the flatting agents, and / or the brightening agents are added into the mixture, and the DBE solutions are added finally. The thermosetting type glass screen printing ink has good adhesion, water resistance, acid and alkali resistance, cold and hot impact resistance, butanone dissolution resistance, fast yellowing resistance, high temperature yellowing resistance, flexibility, constructability and ultraviolet resistance.