The invention belongs to the technical field of plant cultivation and seedling culture, and discloses a strawberry layering seedling culture device, which comprises seedling culture shelves, a parent seedling cultivation groove which is respectively arranged on the top end of each seedling culture shelf and seedling cultivation grooves which are respectively arranged at two sides of the seedling shelf and are arranged below the parent seedling cultivation groove; and the parent seedling cultivation groove and the seedling cultivation grooves are respectively connected with a water-fertilizer supplying system. The strawberry layering seedling culture device has a simple structure and is convenient to install. A corresponding seedling culture method is simple to operate, and the labor intensity is low; the parent seedling cultivation groove and the seedling cultivation grooves are away from the ground and are layered in a three-dimensional way, so the land utilization rate is high, the influence of soil-born insects and the soil environment can be avoided, the environment controllability is strong, convenience in production and operation can be realized, and the application prospect is wide; and in the seedling culture method, fertilizer application and water supply for the parent seedling and the seedling can be realized in a drip irrigation way, so the utilization efficiency of the water and the fertilizer for culturing the seedling can be improved, and in addition, regular and quantitative water and fertilizer supply can promote the growth of the parent seedling and the seedling, the seedling is strong and even, the breeding coefficient is high, and the modernized and industrialized seedling production can be realized.