58 results about "Anaphase" patented technology

Methods and apparatus are provided for the automated analysis of images of living cells acquired by time-lapse microscopy. The new methods and apparatus can be used for the segmentation, classification and tracking of individual cells in a cell population, and for the extraction of biologically significant features from the cell images. Based upon certain extracted features, the inventive image analysis methods can characterize a cell as mitotic or interphase and / or can classify a cell into one of the following mitotic phases: prophase, metaphase, arrested metaphase, and anaphase with high accuracy.

Image analysis methods analyze images of cells and place the cells in particular cell cycle phases based upon certain features extracted from the images. The methods can also quantify the total amount of DNA in a cell based on specific features such as fluorescence intensity from fluorescent molecules that bind to DNA. Further, the methods can characterize a cell as mitotic or interphase based on chosen parameters such as the variance in intensity observed in a cell image and / or the size of a region containing DNA. In one example, image analysis methods can classify the cell into one of the following five phases: G1, S, G2, telophase, and an early stage mitotic phase comprised of prophase, metaphase, and anaphase.

The invention relates to a bacillus subtilis fermentation production technology, which comprises the following steps: 1)inoculating bacillus subtilis in an aseptic culture tube, performing shaking culture and activating to obtain liquid as a first fermentation seed liquid; 2)inoculating first fermentation seed liquid in an improvement medium, continuously performing shaking culture and activating to obtain liquid as a secondary fermentation seed liquid; 3)inoculating the activated secondary seeds in a fermentation medium for fermentation culture, culturing in a fermentation cylinder; and 4)centrifuging a broth at 4000-8000rpm and collecting thalline, and drying at temperature of 30-50 DEG C. The fermentation production technology has the beneficial effect that glucose is employed as a main medium component of carbon source, and thalline growth rate can be grearly increased by continuous feeding and fermentation through dissolved oxygen linkage at anaphase. The bacillus subtilis thalline powder has the advantages of low cost, simple fermentation technology and short production period, and has wide production application prospect.

The invention relates to the technical field of fish propagating, in particular to a technique of artificial insemination and hatching zygote for whitebait and is provided with 1. artificial insemination process: extruding semen and an egg granule in a container, adding normal saline of 0.004 to 0.008 percent, stirring and standing for 2 to 3 minutes; 2. fertilized egg hatching process and prophase hatching: the fertilized eggs are arranged in a hatching chamber and is poured into a hatching tray after sterilized by the normal saline of 0.004 to 0.008 percent and hatched by adding purifying water; the temperature of the hatching chamber is 16 to 20 DEG C, avoiding air, lighting and avoiding from being directly irradiated by sunshine and changing water as well as controlling the water temperature; anaphase management: after the fertilized eggs begin to emerge, stopping changing water and sterilizing, adding purifying water and arranging the fries beginning to swim flatly into a culture pool after 48 to 72 hours. The technique of the invention adopts a reasonable and scientific method and has the processes of sterilizing, water quality control, temperature control, appliance control and environment control, etc., which greatly improves the yield and quality of the fries.

The invention provides a method for effectively reducing environmental risk caused by pollen propagation of transgene plant. In the method, two tightly linked gene expression cassettes of 1) a pollen lethal gene ZM-AA1 driven by a pollen growth anaphase singular promoter PG47 and 2) a fluorescence screening mark gene FP driven by a callus / seed coat singular promoter END 2 are transferred to a Zhonghua 11 wild type material. During selfing fructification of a single plant carrying a single copy transgene, a pollen grain without carrying the transgene can be fertilized with a female gamete normally, but a pollen grain carrying the transgene is abortive in pollen growth anaphase and can not be fertilized with a female gamete, so as to reduce risk of transgene drift in the environment.

The invention relates to a multi-functional peanut growth inhibitor which is composed by the weight ratio of following components, paclobutrazol to prohexadione calcium to uniconazole is 3: 1.2-1.5: 0.8-1.2. The invention not only can effectively control the growth of the vegetative part of the peanut on the ground, can prevent the peanut from overgrowing and lodging, but also can effectively avoid the side effect brought by general growth inhibitor (e.g. paclobutrazol). At the same time, in the growth anaphase of the peanut, the invention can remarkably improve LAI (leaf area index), effective LAI, leaves membrane-lipid protective enzymatic activity (SOD, POD, CAT), Pr (soluble protein) content and so on, can reduce MDA content and can postpone the senescence rate of peanut leaves. More importantly, the invention can remarkably improve the output of pods. The production can be increased by 16.6 percent- 23.0 percent more than the production without spraying any inhibitor, and can be increased by 7.6 percent- 13.7 percent more than the production only spraying paclobutrazol. The invention solves the problem that the overgrowth and presenility can not be prevented synchronously in the production process of the peanuts.

The invention provides a breeding method for high-oleic acid high-yield peanut, which includes steps: (1) coordinate parent combination: selecting high-yield germplasm with good integrated character and germplasm with high oleic acid content SPI098 to combine patent; (2) high oleic acid pressure screening: determining oleic acid of each seed in excellent single plant in early separation generation, and selecting excellent peanut seed with high oleic acid content; (3) high-yield pressure screening: planting the screened peanut seed with high oleic acid content in next generation, separating generation in anaphase, and selecting excellent high-yield single plant; and (4) high-oleic acid high-yield peanut variety or strain obtaining: obtaining novel high-oleic acid high-yield peanut variety or strain through multi-generation high-oleic acid high-yield pressure screening. The breeding method provided by the present invention directly screens according to character of oleic acid content in peanut seed, and has advantages of short breeding period, small blindness, high efficiency, and good economic benefit and social benefit.

The invention discloses a cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease. At first, under heterotrophic culture condition, the cells are stimulated to grow rapidly by utilizing fulvic acid; when the microalgae cells grow to the anaphase of logarithmic phase, cell concentration reaches 6.63g L<-1> at the moment; supernatant is removed through centrifugation so as to serve as a seed solution required in a subordinate phase. A 1000mgL<-1> fulvic acid mother solution is prepared by using pure water, and the fulvic acid mother solution is added in kuh1 culture mediums, so that the concentrations of the fulvic acidare 5, 25, 125, and 625mg L<-1> respectively. Then the obtained seed solution is added in the kuh1 culture mediums containing the fulvic acid of different concentrations, and the initial concentration of the seed solution is 2.3g L<-1>. Then the algae solution is subjected to continuous illumination cultivation, and sampling is performed every two other days for measuring biomass and grease content. The cultivation method disclosed by the invention is simple and feasible, and low in cost; the cultivation time is greatly shortened and the grease content is remarkably increased, and great significance is put forth for the industrialization of microalgae biodiesel production.

The invention discloses a method for producing L-tyrosine through an enzyme method. The method comprises the following steps of adopting a fermentation method to produce pyruvic acid, adding beta-tyrosinase, phenol and NH4Cl at the fermentation anaphase of the pyruvic acid, converting the generated pyruvic acid into the L-tyrosine, and obtaining fermentation liquor containing the L-tyrosine; heating the fermentation liquor containing the L-tyrosine to be 70 DEG C to 90 DEG C, and acidifying until L-tyrosine crystals are completely dissolved; adding activated carbon to decolor, after decoloring, filtering by utilizing a ceramic membrane, removing bacteria, albumen and the activated carton, carrying out secondary filtering on obtained clear liquid through an acid-resisting liquid cartridge filter; adjusting the pH of the secondary filtered clear liquid to be 5.0 to 7.0, cooling to crystallize, centrifuging, and obtaining the L-tyrosine. According to the method for producing the L-tyrosine through the enzyme method provided by the invention, the process is simplified, the production efficiency is improved, the obtained L-tyrosine is high in purity and yield, the purity of the obtained tyrosine is remarkably improved and reaches to 98.0 percent or above compared with a traditional extraction method, and various tyrosine drug standards are met.

The invention discloses a method for efficiently regenerating a Sapium sebiferum leaf disc. The method comprises the following steps: cutting leaves of aseptic Sapium sebiferum seedlings into leaf discs, and conducting induction culturing on the leaf discs through a cluster bud induction culture medium to form cluster buds; conducting micropropagation and elongation on the cluster buds in a micropropagation and elongation culture medium; and after the buds grow to 2-3cm, transferring into a rooting culture medium for rooting culturing, thus obtaining strong Sapium sebiferum test-tube plantlets after 25-30 days. Compared with the prior art, the method has the following advantages that raw materials are easily available and abundant, the regeneration efficiency is high, the culture period is short, hormone components added to the culture mediums are simple, the operation is convenient, and the like, and the method can realize fast propagation of excellent wild single plants, and keep the germplasm characteristics of a female parent, thus realizing the breeding of variety, laying a foundation for the genetic transformation of Sapium sebiferum, being beneficial to quickening the genetic improvement of Sapium sebiferum, and providing technical support for the fast propagation of excellent anaphase Sapium sebiferum plant systems in the later period.

The invention belongs to the agriculture plantation technical field, and discloses a hybridization Broussonetia papyrifera seedling growing method comprising the following steps: 1, matrix disinfection: loading the matrix into a seedling growing container, using 75% quality concentration alcohol to disinfect the matrix, using the quality concentration 3% limewash to disinfect the matrix, using a film to cover the matrix for preparation; the matrix is formed by mixing the following raw materials by weight parts: slow release fertilizer 10-15 parts, activation rock phosphate 8-12 parts, medium sand 5-10 parts, husk 5-10 parts, humus soil 3-8 parts, diatomite 3-5 parts, earthworm powder 3-8 parts, rock wool 3-5 parts, chitosan 3-5 parts, and puffed chicken manure 3-5 parts; 2, cutting branch selection and processing; 3, cutting; 4, cutting anaphase management. The seedling growing stock obtained by the method is high in survival rate, and the seed selection breeding Broussonetia papyrifera is suitable to promote and plant in rocky regions in our nation.

The invention discloses a method for smelting high-carbon and low-phosphorus steel by utilizing a converter at a low temperature. The method for smelting the high-carbon and low-phosphorus steel by utilizing the converter at the low temperature comprises the following steps: (1) carrying out blowing prophase control; (2) carrying out blowing metaphase control; and (3) carrying out blowing anaphase control. The method for smelting the high-carbon and low-phosphorus steel by utilizing the converter at the low temperature has the advantages that low temperature tapping is adopted, the problem that end points [C] and [P] can not meet tapping conditions at the same time can be effectively solved, furnace life of the converter can be improved, and refractory material consumption is directly reduced; a single slag process is adopted for slag-making, a process route is simple, safety and operability are strong, and easy control is realized; high tapping at the end point [C] is realized, oxygen content during tapping is reduced, and alloy consumption is reduced while quality of molten steel is improved; and pure oxygen supply time of the converter can be shortened by about 2 minutes, so that production efficiency of the converter is improved.

The invention discloses an experimental apparatus for in-vitro circulating perfusion and digestion of liver tissues. The experimental apparatus comprises an incubator 1 and a perfusion loop arranged in the incubator 1, wherein the perfusion loop comprises a liver-tissue container 2, a peristaltic pump device 4, a three-way pipe 5, a filtering device 6 and an aseptic pipeline 7 which are connected in sequence; the peristaltic pump device 4 comprises a peristaltic pump 3 and a flexible pipeline 11 corresponding to the peristaltic pump 3; the flexible pipeline 11 bypasses the corresponding peristaltic pump 3 and the two ends of the flexible pipeline 11 are respectively communicated with the liver-tissue container 2 and the three-way pipe 5. The experimental apparatus disclosed by the invention can be applied to separation of various cells in the liver tissues, can digest collagenous tissues in the liver tissues by a circulating digestion mode to enable the cells to be dispersed, and collect the cells for the following culture and realizes related experimental research on the biological characteristics of various cells in the liver tissues; since the cells are separated out in shorter time, the activity of the separated-out cells is higher and the success ratio of anaphase culture is increased.

The invention discloses a fertilization method under the condition of drip irrigation cultivation under corn film, which belongs to the field of agricultural planting technology. The method is: mechanical fertilization, the fertilizer applicator is set with three fertilization ports, three rows of fertilizer are applied on each large ridge, and the middle row of fertilizer is applied to the ridge In the middle, the distance between the fertilization openings on the outermost two sides is 50-55cm; apply 60% fertilizer in the middle, and 20% fertilizer on each side; apply 15-17cm deep in the middle, and 8-10cm deep on both sides place. The beneficial effects are as follows: 1. Cross-layer fertilization. In the early stage of corn growth, its root system is short and can absorb shallow fertilizer, while in the middle and late stages of corn growth, its root system is longer and can absorb deep fertilizer; 2. Fertilize on both sides. There are fertilizers on both sides of the corn plant, and the closest distance to the fertilization belt is 5cm, which fully exerts the absorption of the fertilizer by the whole plant and improves the utilization rate of the fertilizer; 3. Fertilize on demand. Corn needs more fertilizer at the jointing and booting stage. At this time, the root system is longer and can absorb deep-level fertilizers, and the deep-level fertilizers account for a large proportion, which can just meet the needs of corn for fertilizers.

It relates to an method of intermediate rearing mussel germchits during later period in shallows. When the germchits are cultivated during later period at natural cultivating sea zones, the germchitsare cultivated in string bags, and hanged at buoyant rafts and cultivated. The method comprises: transferring juvenile mollusks with the 0.4??.5mm shells in length into string bags, 5,000í½11,000 juvenile mollusks in a string bag, assembling 6í½10 bags into a bundle, assembling 10-15 bundles into a string, the distance between strings is 15í½30cm; the distance between the string ropes is 1.0í½1.5mú

The invention relates to a plant lactobacillus, in particular to a method for improving the stability of the plant lactobacillus. The plant lactobacillus is the plant lactobacillus of LP-Onlly; NaCl is added during the anaphase of fermentation logarithm phase to carry out high temperature processing or low temperature processing so as to improve the stability performance of the cell of the plant lactobacillus. The invention adopts a microorganism metabolizing engineering strategy and mainly utilizes two pressure stress processing technologies of penetration and temperature to improve the stability performance of the plant lactobacillus which provides a method with practical meaning for improving the stability of the lactobacillus, thereby improving the quality of a product, providing the processing feasibility of a normal temperature probiotics product and prolonging the service life of a product shelf.

The invention discloses a chromosome preparation method, as well as a required culture medium and a preparation method thereof, and is used for solving karyotype analysis problem of chromosome. The culture medium consists of RPMI (Roswell Park Memorial Institute) 1640, heparin sodium, HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid), L-glutamine, NaHCO3, benzylpenicillin potassium, streptomycin sulphate, bovine serum and phytohemagglutinin (PHA). The detection method comprises the following steps: implanting 0.3 to 0.4ml of human peripheral blood into the culture medium; adding colchicinamide in 2-4 hours before culture is terminated to realize that the cell is terminated in anaphase; culturing the cell after 68 to 72 hours to harvest the cell; performing hypotonicity for 40 minutes, three times of fixation, banding, dyeing and other treatments; and performing chromosome analysis under a microscope to determine whether the peripheral blood supplier has a phenomenon of chromosome abnormality. The culture medium disclosed by the invention has convenience for use, simpleness in operation, low cost and low patient detection fee, and is suitable for genetic diagnosis, infertility and prenatal diagnosis in each level of hospitals.

The invention relates to the medical technical field of repairing wounds, which can be used for repairing wounds, particularly for healing deep burn wounds. The invention provides mesenchymal stem cell suspension which contains human epidermal growth factor and bovine basophilic fibroblast growth factor. The mesenchymal stem cell provides the sources for human marrows or embryo. When in use, the stem cell suspension is directly injected or smeared on wounds which have been removed necrotic tissues. Animal experiments show that the stem cell suspension can obviously repair deep burn wounds; besides, the new skin does not tend to generate blister or ulceration. The stem cell suspension of the invention is simple in preparation and conveniently for use, obviously repairing the deep burn wounds, thus effectively controlling the hyperplasia of anaphase keloid.

The invention belongs to the biological information technology field, and particularly relates to a non-coding RNA analysis prediction method combining a base fragment obtained by Illumina second-generation sequencing with PeakCalling method. The method comprises the steps of rejecting rRNA bacteria second-generation sequencing data; after obtaining the data, analyzing the data by conducting pollution removing and low quality removing analysis of the data to obtain Clean reads first; then comparing the reads onto a bacteria genome; predicting a transcription unit preliminarily; filtering noted mRNA and ncRNA to obtain predicted ncRNA; and noting the ncRNA to a known ncRNA database Rfam to obtain a final prediction result. The unnoted ncRNA in the bacteria genome can be predicted accurately, making up the insufficiency of experiment means, and providing a favorable support for anaphase experiment and scientific research.

The invention provides a method for original ecological large-scale planting of typhonium giganteum. The method comprises the following steps: A, selecting a planting site; B, planting a woodland artificially; C, performing plowing; D, performing soil preparation; E, planing typhonium giganteum; F, performing seedling management; G, performing irrigation and fertilization; H, performing weeding; I, picking flower buds; J, reserving seeds for planting; and K, performing harvesting. According to the method provided by the invention, the typhonium giganteum is planted in the woodland with a natural cover and without pollution, is fertilized with an organic fertilizer, a humus fertilizer, a base fertilizer and a planting matrix are applied to soil in a prior period, the soil environment for planting is improved, the use level of top dressing in the anaphase is greatly reduced, the natural property of the medicinal material is improved while the cost is reduced, and moreover, the medicinal material is high in yield, strong in medicine property and excellent in quality.

The invention discloses a grice forcing supplementary feeding method and relates to the technical field of scientific cultivation. The grice forcing supplementary feeding method is a wholly new supplementary feeding method which is designed for the purposes of accurately controlling the quantity and time of grice supplementary feeding, achieving the ideal supplementary feeding effect and reducing the harm caused by the 'early ablactation syndrome' of grice. The technical points are that the grice supplementary feeding period is 8-21 age in days, in the supplementary feeding period, 'free feeding and forcing supplementary feeding' are carried out, the 'teaching feedstuff' with high quality is selected as the supplementary feedstuff, the times of supplementary feeding is 4 times every day, and the total amount of supplementary feeding is 290g. The supplementary feeding course is divided into three stages: 8-11 age in days is the training period, the dosage is increased from less to more and is 10g per day averagely; 12-16 age in days is the middle period, and the dosage is 20g per day averagely; 17-21 age in days is the anaphase, the dosage is 30g per day averagely. By adopting the method provided by the invention, the 'early ablactation syndrome' can be alleviated obviously, disease rate and death rate of the grice can be reduced, and the growth state of the grice can be improved.

The invention discloses a eustoma grandiflorum cultivation method comprising the following steps: selecting field, soil working, applying fertilizer, disinfecting soil, and applying fertilizer; removing underground insects; using a single-particle-sing-hole mode to sow eustoma grandiflorum seeds into deep hole trays filled with a sowing matrix, wherein the sowing matrix is excellent in draining performance; examining seedlings when the eustoma grandiflorum seedling grows to 3cm above the ground, removing a film and transplanting the seedlings when the eustoma grandiflorum seedlings grow to 5-10cm in height and the roots grow to 8-10cm in depth; weeding when the seedlings grow to 12cm, and weeding in every two months; topdressing; timely removing top buds when the seedlings grow to 10cm. The method can improve the eustoma grandiflorum germination capacity, and the produced eustoma grandiflorum seedlings are stronger, thus improving the seedling transplanting survival rate; the anaphase growth vigor is fast, and the product quality is good; cut flower eustoma grandiflorum anaphase growth consistency is good, and the flower leaves are tidy; the eustoma grandiflorum is strong in disease resistance, thus reducing insect diseases, improving cut flow yield, and improving the economic benefits.

A method for producing gingko scented tea comprises the following steps: 1 picking alabastrum and plucking episodes, 2 removing impurities, 3 drying in the sun, 4 protecting color and firming the shapes, 5 increasing flavor ((1) oven-drying, (2) increasing flavor in prophase, and (3) increasing flavor in anaphase), 6 preparing to obtain gingko scented tea and composite gingko scented tea. Gingko scented tea of the invention can be drunk not only singly but also with gingko leaves and other tea leaves, and is an ideal body building drink with fresh taste, heavy flavor, and natural body building value.

The invention relates to a kind of culture medium for cultivating insect grass and the preparation method. The culture medium mainly contains slabby admixture which is composed by aging corn and chrysalis. The weight ratio in the admixture follows this. Carbon is 0.4%-1.0%, while nitrogen is 0.02%-0.05%. The surroundings will change when bacterium grown up in alimentation period of growth and anaphase by this method, so the technique for preventing and diffusing light is needed. Thus, the content of sterine, nucleoside and organic acid in the insect grass is the same to or more than content in the wild. At the same time, the output of sporocarp can increase by 10%-15%.

A common vetch herbage cultivation method comprises the following steps: 1, using common vetch and cruciferous rape or broad bean for mixed spawning, wherein common vetch and cruciferous rape mixed spawning plant ratio is 10:0.6-3, and common vetch and broad bean mixed spawning plant ration is 4:1-2; 2, broadcast spawning the seeds into field, immediately using clear water to irrigate, and a mud pile is placed in a field water inlet so as to stir the water for irrigation. The method can effectively prevent lodging of the common vetch plants in a growth anaphase, can prevent the common vetch seeds from being picked up by birds in broadcast spawning, and the seeds cannot be dead because of strong light exposure, thus improving emergence rate, and economic benefit is obvious.

The invention relates to a strategy for producing acetoin through efficient fermentation based on AR / BDH enzymatic properties of bacillus subtilis and belongs to the field of genetic engineering and fermentation engineering. Shown by researches on the AR / BDH enzymatic properties of a strain B. subtilis JNA, which is independently screened by our laboratory, has independent intellectual property and can produce acetoin at high yield, the conditions that the optimal pH in the reduction direction of AR / BDH is 6.5 and the optimal pH of the oxidation direction of AR / BDH is 8.5 are discovered for the first time. Based on the enzymatic properties and according to the fermentation strategy, the strain is in the optimal growth pH (6.5) condition at the prophase of fermentation and in the optimal transformation pH (8.0) condition at the anaphase of fermentation, 150g / L glucose can be transformed into 56.8g / L acetoin, the yield of 2,3-butanediol is only 6.1g / L, and the production rate of acetoin reaches 0.59g / (L.h) and is increased by nearly 84%; recombinant B. subtilis which overexpresses AR / BDH consumes 180g / L glucose under the regulation and control of the same fermentation strategy, the yield of acetoin is increased to 73.6g / L, and the production rate finally reaches 0.77g / (L.h), so that the capability of producing acetoin through the anaphase reverse transformation of the target strain is enhanced, and a basis is provided for producing acetoin through microbial industrialized fermentation.

The present invention discloses a new application of E4BP4 gene, it can be used as regulator for regulating embryo nidation (embryo implantation). In prophase of embryo nidation it can block expression of E4BP4 gene or close the biological function of its expression product E4BP4 protein, so that it can inhibit embryo nidation, otherwise it can promote nidation; in anaphase of embryo nidation it can block expression of E4BP4 gene or close the biological function of its expression product E4BP4 protein, so that it can promote embryo nidation, otherwise, it can inhibit nidation.

The invention provides a DNA bar code for discriminating aegilops tauschii and aegilops cylindrica and an application thereof. A nucleotide sequence of DNA bar code is shown in as SEQ ID NO: 1-2. By designing a pair of novel DNA bar code primers, an optimal identification sequence is obtained, the DNA bar code sequences of aegilops tauschii and aegilops cylindrica in a Genbank database are improved, aegilops tauschii, aegilops cylindrica and aegilops umbellulata can be distinguished by only one pair of the primers, and the unknown aegilops tauschii and aegilops cylindrica can be rapidly identified. Compared with a form characteristic identification method at a growth anaphase, the DNA bar code breaks up the restriction of an identification period, greatly increases the identification efficiency, increases the detection speed and accuracy, is in favor of guidance for timely controlling two wheat field worst weeds for an agriculture production mechanism and peasants, ensures the production safety of wheat and peasant income, reduces the threat to national food security, and has good economic benefit and social benefit.

The invention is concerned with the middle-degree pruning method for lateral branch, the characteristic is: the woods is the crown closure period or near crown closure when the anaphase young growth and the incipient period half-mature forest, process the middle-degree pruning to the lateral branch (expect the 1 to 2 years top branch, namely pruning of the 1 / 3 to 2 / 3. After three years and the first pruning, the forest enters the crown closure period or near crown closure again, processes the pruning again until the forest enters the anaphase of the half-mature forest. The invention can extend the space between the crowns of the tree, improves the light irradiation state and the light energy utilization ratio.