The invention belongs to the technical field of
plant tissue culture, and particularly discloses a method for
transplanting and hardening
tissue culture seedlings of rabdosia nervosa. The method for
transplanting and hardening the
tissue culture seedlings of the rabdosia nervosa comprises the following steps of (1) selecting seedlings with roots; (2) performing pretreatment before
transplanting; (3) proportioning transplanting mediums; and (4) controlling a
seedling-hardening environment: putting the transplanted rabdosia nervosa into a
greenhouse for
seedling hardening, and adjusting and controlling light, temperature and
humidity. The method can effectively solves the phenomena that water losses are severe, the
survival rate is low and the commodity rate is low during transplanting and
seedling hardening of the rabdosia nervosa; by proportioning the different mediums, cost can be reduced, the seedling transplanting flow is simplified, and the
survival rate of seedling transplanting reaches up to 95%; by controlling external conditions of seedling hardening after transplanting, the problem that the water losses of the tissue culture seedlings of the rabdosia nervosa are severe canbe effectively solved, and thereby, commodity rate of the seedlings is increased; the market requirements can be effectively met; and a plenty of high-quality rabdosia nervosa seedlings are rapidly supplied for markets.