The invention discloses a breeding and propagation method of schizothorax prenati. The method comprises the following steps of 1, building a parent fish breeding pond; 2, using running water to stockparent fish, wherein 3-5 day-old healthy schizothorax prenati is selected as the parent fish, and the parent fish is placed into the parent fish breeding pond to be stocked in the running water; 3, conducting artificial spawning induction on the parent fish, wherein March to May serves as a propagation season, the water temperature reaches 9-15 DEG C, and the parent fish with high gonadal maturityare subjected to spawning induction; 4, conducting artificial insemination and incubation. According to the breeding and propagation method of schizothorax prenati, a natural habitat environment of schizothorax prenati is simulated in the breeding pond for breeding the parent fish, artificial spawning induction, insemination and incubation are conducted, the spawning induction rate of the male parent fish reaches 85% or above, the fertility rate is around 90%, the incubation rate is around 80%, and the breeding and propagation method is superior to other methods for propagating schizothorax prenati, and is very significant in improving the artificial propagation effect of schizothorax prenati.