40results about How to "Increased labor induction rate" patented technology

The invention relates to a totally artificial breeding method for Coilia ectenes, belonging to the technical field of fish culture. The method is characterized by comprising the following process steps of parent fish source; parent fish select breeding comprising standing / flowing water combined reinforced breeding; artificial induced spawning measure comprising parent fish selection, semen collection, dug roe inspection, induced spawning water temperature confirmation, parent fish matching, hormone injection, artificial insemination and the like; microflow water incubating; and summer flower and fish fry breeding. The invention promotes the parent fish sex gland to grow to maturity and ovulate by measures of reinforcing parent fish nutrition reinforced breeding, feeding hormone, flowing water stimulating and regulating and the like and normalizes the artificial induced spawning and insemination process, originally creates a simple flowing water incubating device of a pelagic egg and reinforces the management and ecological regulation of incubation period, thereby effectively solving the technical problems of artificially breeding Coilia ectenes. The induced spawning rate of artificial breeding is 33 percent, the fertilization rate is 48 percent and the hatching rate is 44 percent.

The invention discloses a percocypris scaled artificial propagation method. The percocypris scaled artificial propagation method comprises the following steps of (1) culturing of parent fishes: using a flowing water pond as a parent fish culturing pond to culture; (2) distinguishing of male and female parent fishes: utilizing a B-ultrasonic scanner to check the abdomens of the parent fishes to distinguish the male and female parent fishes; (3) artificial maturation induction: injecting luteotropin into the distinguished female fishes to release hormone A2 to accelerate induction; (4) artificial spawning induction: performing artificial spawning induction on the selected male and female parent fishes; (5) artificial inseminating; (6) hatching: performing the flowing water and light shielding hatching on the fish spawns after artificial inseminating in a yarn silk hatching framework; (7) culturing of fish fries: when the hatching fish fries start to flatly swim, using the fairy shrimps or crushed water earthworms as an opening bait; after the fish fries are completely domesticated to eat an artificial matching feed, moving the fish fries to a fish fry culturing pond to culture. The method has the advantages that the implementation is easy, the operation is simple and convenient, and the distinguishing rate of the male and female parent fishes can reach more than 90%; the spawning induction rate of the matured parent fishes which are artificially cultured reaches 75%.

The invention relates to an artificial propagation method of fry of paracobitis variegatus. The artificial propagation method comprises the steps of parent fish rearing, parent fish selection and mating, artificial spawning, fertilization, oosperm hatching, larva fish temporary rearing and fry rearing. The artificial reproduction technology of the paracobitis variegates is successfully summarized in the artificial propagation method, and the artificial propagation method fills the blank of the technology. By adopting and popularizing the artificial propagation method having been successfully studied out, the fry of the paracobitis variegatus can be obtained in batches in a centralized mode, the spawning rate, the fertilization rate and the hatching rate are high, and large-scale production of the paracobitis variegatus can be easily achieved. Thus, the artificial propagation method has great significance on protection and large-scale rearing of the paracobitis variegatus wild resources.

The invention belongs to the technical field of aquaculture, and particularly relates to a breeding method for increasing the survival rate of loaches manually bred in a factory-like mode, wherein the breeding method is reasonable, simple and high in operability. According to the breeding method, the natural mating method, the sperm extrusion method and the laparotomy spermatophore-obtaining method can be adopted, the death rate of parent fish for breeding can be greatly reduced, and the spawning induction rate, the fertilization rate and the hatching rate can be increased.

The invention discloses a method for batch breeding of loach fry, which notably improves catalytic production rate of the loach. The method comprises the steps of: a, selecting loach parent for breeding to perform prenatal strengthening cultivation; b, building a third-level filtering reservoir device for spawning and hatching; c, selecting composite of the maleic acid domperidone and the trout luteinizing hormone releasing hormone analogue as a catalyst to catalyze male and female loaches, wherein per kilogram of female loaches need 10-15mg of the catalyst; per kilogram of male loaches need 5-7.5mg of the catalyst; enabling the female loaches and the male loaches to spawn and inseminate in a net cage in the jar according to the ratio of the female loaches to the male loaches being 1:2; taking out the loaches, enabling the spawns to continuously hatch; producing loach fry in a large scale; d, feeding the initial feed, putting the loaches to the pond; according to organ development characteristic of the loach fry, controlling diet conversion time point, electing palatable bait, scientifically feeding the bait, and strengthening water quality and disease control and prevention.

The invention discloses a large-scale common sucker breeding method capable of controlling the temperature of circulating water. The method comprises the following steps of parent fish selection, parent fish rearing, artificial spawning induction, artificial fertilization, artificial incubation, larva fish cultivation, disease control and the like. In an artificial breeding process, the temperature of the water is controlled in a range of 15-20 DEG C by using a circulating water temperature controlling technology, the water is circulated and is used efficiently, the prosperous breeding period of common suckers is greatly prolonged, adverse effects on artificial breeding due to over low water temperature in the early stage and over high water temperature in the later stage are avoided, and the problems that the water quantity is insufficient, the water quality is deteriorated and the water is polluted in the breeding period are solved. According to the technical scheme obtained from scientific experimental research and production practice, the spawning induction rate, the spawning rate, the fertility rate, the hatching rate and the emergence rate of the common suckers can be greatly improved, the bottleneck on large-scale breeding of the common suckers is solved, and the large-scale common sucker breeding method plays an important role in scientific research, population protection and artificial breeding of the common suckers.

The invention provides a hybrid carp and pengze crucian carp hybrid breeding method and belongs to the technical field of freshwater fish hybrid breeding. The hybrid carp and pengze crucian carp hybrid breeding method aims at solving the problem that no proper hybrid method exists in the prior art to prevent carp diseases in the northern area. The method includes the steps that a first filial generation is obtained by regarding a German mirror carp as a female parent and a jian carp as a male parent for hybridization, an irregular male carp with the high growth speed and a few large body surface scales serves as a male parent, a batch of plump female pengze crucian carps with more than 31 lateral line scales and high growth speed are optimized out through two-generation breeding to serve as a female parent, parent mature promotion and artificial propagation are carried out on the basis of optimizing and directive breeding of hybrid parents, hybrid carps coincident with the pengze crucian carp female parent in appearance can be obtained after distant hybridization is carried out, hybrid generation has no carp characters, and the hybrid carp and pengze crucian carp hybrid breeding method has the advantages of being high in growth speed and disease resistance, and is of great significance in improving unit yield, increasing breeding benefits, breeding new species and achieving large-scale fry rearing.

The invention relates to the technical field of aquaculture, in particular to an artificial breeding method for acipenser dabryanus. Oxytocin is injected into female acipenser dabryanus two times, wherein the interval between two times of injection is 12 hours, and the injection amount of the first time accounts for 1 / 3 to 1 / 2 of the total dose. The one-time injection method is adopted for male acipenser dabryanus, wherein injection of the male acipenser dabryanus is synchronous with the first time of injection of the female acipenser dabryanus, and the dose of the male acipenser dabryanus is half that of the female acipenser dabryanus. Fertilization is conducted according to a half-dry method, water, eggs and seminal fluid are mixed according to the proportion of 75:50:1, and the mixture is stirred for 2 minutes to 3 minutes. A complete acipenser dabryanus artificial breeding technique process is provided, and technical support is provided for large-scale artificial breeding; due to the adoption of the method, the hatchability of acipenser dabryanus can reach 85% or higher at most, and restrict factors in the acipenser dabryanus artificial breeding process are eliminated.

The invention discloses an artificial propagation method for coreius guichenoti. The method includes the steps of 1, selection of parent fishes, wherein parent fishes the gonads of which are high in development maturity are selected to serve as spawning acceleration parent fishes according to external features of the parent fishes of coreius guichenoti and the eggs dug out by an egg digging device; 2, preparation and injection of spawning acceleration drugs, wherein according to the weight of the fish bodies, normal saline is adopted for preparing the spawning acceleration drugs such as lutropin releasing hormone A3, gonadotropin of fish pituitary glands and chorionic gonadotropin, a two-time injection method is adopted for injection, the water temperature is 20.5-22 DEG C, and the needlepitch is 8-10 h; 3, artificial propagation acceleration and insemination, wherein the parent fishes are checked at irregular time after injection of the spawning acceleration drugs, artificial insemination is carried out once the phenomena of ovulation and spermiation occur, and a wet insemination method is adopted as the insemination method; 4, hatching, wherein the obtained fertilized eggs are poured into a conical hatching device for hatching, and after fry get out of membranes, the fry are moved into a fry cultivation box for fry cultivation. The operation is simple, the artificial propagation rate and the survival rate of the fry are effectively increased to 85% or above, and a solid base is laid for propagation, releasing and protection of coreius guichenoti group sources.

The invention relates to parturition hastening methods for shellfish products, in particular to a method for hastening parturition of shellfishes by sterilized and neutralized sea water. According to the method, outdoor sea water is sedimented in a sedimentation tank, sodium hypochlorite or bleaching powder are added for sterilization, sodium thiosulfate is used for neutralizing residual chlorine in the water, water in a nursery pond is firstly drained off during parturition hastening, the neutralized sea water is injected into the nursery pond until the sea water submerges seed shellfishes, air inflation and oxygenation are performed, the seed shellfishes are added immediately once spawning of the seed shellfishes is discovered, and the sea water is injected until the pond is filled with the sea water. The method is simple, and easy to implement. The method has the advantages of short time, high parturition hastening rate and good effect in terms of shellfish parturition hastening. The method belongs to methods for changing growing environmental conditions of the seed shellfishes, but the method is slow and gentle and does not cause intense stimulation and damage to the seed shellfishes. Indexes such as fertilized-egg hatching rate, metamorphosis rate and blood-shellfish repeating utilization factor are increased by 20% as compared with those in the prior art. The problems of shellfish source shortage and difficulty in seed breeding during shellfish product production are solved indirectly.

The invention discloses a total artificial propagation method of opsariichthys bidens. According to the method, parent fish is selected from first-generation and second-generation artificially cultivated groups of wild opsariichthys bidens subjected to artificial domestication and breeding, artificial cultivation is performed until opsariichthys bidens gonad develops maturely, and therefore the parent fish individuals have good maturation synchronism, and the maturation rate is higher; then hormone HCG is used for spawning induction, the spawning induction effect is good, and the spawning induction rate is high; physiological saline is used for activating spermatozoa during artificial insemination, the fertilization effect is improved, and the fertilization rate is higher; and fertilized eggs are put in an incubation frame in an incubation device, hatching is carried out under temperature control conditions, and therefore the hatching effect is improved and a high hatching rate is achieved. According to the method of the present invention, improvement and innovation are carried out on the prior art to realize the full artificial propagation of the opsariichthys bidens. The method focuses on technical links and refinement of technical parameters so as to facilitate popularization and use. The technical method of the present invention is verified by multiple production, and the operability and practicality are high.

The invention provides a method for d domesticating wild Leptobotia elongata under artificial cultivation conditions until the gonadal maturation of the Leptobotia elongata. The method includes: building an ecology-simulated pond to simulate the habitat of the wild Leptobotia elongata; subjecting the collected Leptobotia elongata to soaking and sterilizing, and performing isolated temporary cultivation; domesticating the Leptobotia elongata after the temporary cultivation in the ecology-simulated pond; feeding the Leptobotia elongata with prepared parent Leptobotia elongata feed; strengtheningbefore reproduction, to be more specific, three months before the reproduction of the Leptobotia elongata, injecting carp hypophysis cerebri grinding liquid once each month; two months before the reproduction, increasing the flow speed of the ecology-simulated pond, and increasing the water temperature to 25-26 DEG C to stimulate the gonadal maturation of the parent Leptobotia elongata. The method has the advantages that the habitat, water flow and food of the wild Leptobotia elongata are simulated, the nutrition proportion of the parent Leptobotia elongata feed is regulated, the strengthening before reproduction is performed, gonadal maturation rate can reach above 50%, and spawning induction rate during large-scale artificial reproduction is increased.

The invention relates to the field of beluga culture, particularly discloses a beluga artificial propagation method and application of a carp pituitary gland, and relates to a beluga artificial propagation method. The method comprises the steps that 1, nutrient enrichment culture is performed on a beluga milter parent and a beluga raun parent; 2, the beluga milter parent and the beluga raun parent obtained after nutrient enrichment culture are subjected to low temperature stimulation; 3, the water temperature is increased to the pre-spawning induction temperature, and spawning induction medicine administration is performed on the beluga milter parent and the beluga raun parent so as to be subjected to spawning induction; 4, free eggs and seminal fluid are collected to be subjected to artificial fertilization and incubation, wherein the spawning induction medicine is the carp pituitary gland. The invention further relates to application of the carp pituitary gland for beluga artificial propagation, and application of the carp pituitary gland for preparing medicine for artificial propagation of beluga. The beluga spawning induction medicine is easy to obtain and prepare, the spawning induction rate of spawning induction propagation performed by adopting the beluga spawning induction medicine is high, the fertilization incubation effect is good, and the postpartum parent fish survival condition is good.

The invention provides a method for artificial inducing spawning of trachinotus ovatus. The method comprises the following steps: (1) a parent fish is selected, (2) inducing spawning intensified breeding of the parent fish is performed, wherein a water temperature is increased by 0.3-0.5 DEG C every 2-3 days; every 3-5 days, compound seawater is used to replace 6-10% of pool water; a fortified bait is fed 3-4 times each day; and the light is extended for 10-15 min every two days; (3) temperature-controlled injection of oxytocin is performed 3 times for artificial inducing spawning; and after 3times of the temperature-controlled oxytocin injection, a water flow rate is adjusted to 0.8-1.4 m<3> / s, and a light period is prolonged to 15 L / 9 D-16 L / 8 D; and (4) artificial insemination and hatching are performed. In the method, the parent fish is subjected to inducing spawning intensified breeding, and a manner of 3 times of temperature-controlled injection inducing spawning is used, so that the parent fish is subjected to inducing spawning under different water temperature environments, and the water flow rate and light period after injecting oxytocin are increased, so that the parentfish inducing spawning effect is effectively improved, the effect time is shortened, and a fertilization rate and a hatching rate are high.

The invention discloses a reinforced cultivation method of parent fish of schizothorax biddulphi. The method comprises following steps: putting up a fish pond with water surface of 15-20 mu, water depth of 1.5-2.0 m, and transparency being more than 30 cm; breeding schizothorax biddulphi of 10-15 cm in length into the fish pond in the field from April to May and throwing 250 chubs of around 50g and 500 grass carps of around 50g to per mu; mixing lactic acid bacteria aquas, yeast powder, and feed and drying them in the shade according to the proportion and feeding for 3 days of every 15 days and feeding once every afternoon; throwing photosynthetic bacteria liquid once at the time interval of 15 days; and utilizing circulation water to stimulation of schizothorax biddulphi by running water at the beginning of March in the next year.The reinforced cultivation method of parent fish of schizothorax biddulphi has following beneficial effects: survival rate of parent fish is improved and fish growth and fast growth of sexual gland are promoted; spawning induction of parent fish is successful; and population continuity of schizothorax biddulphi is underpinned by the reinforced cultivation method.

The invention discloses an artificial breeding method of spiny eels. The artificial breeding method includes: at the peak stage of breeding each year, namely a period from the middle of May to the beginning of July and September, selecting parental fishes whose sexual maturity is high from the spiny eels which are captured outdoors or domesticated, where female-male ratio is 1:1.2; injecting artificial induced spawning drug into the parental fishes, taking semen of male fishes and eggs of female fishes for artificial insemination, using brine shrimp nauplius for mouth opening after hatching, using Tubificidae to feed for one month, and starting to use mixed feed for domestication when the spiny eels grow to about 3 cm. The artificial breeding method has the advantages of high induced spawning rate, high fertilization rate, high hatching rate, high survival rate, low parent death rate, suitability for large-scale fingerling breeding according to needs, high efficiency, simplicity, convenience and conduciveness to popularization.

The invention discloses a low-loss and efficient artificial breeding method of Taiwan loaches. The low-loss and efficient artificial breeding method is characterized by comprising the following stepsof 1, pre-delivery breeding of breeding loaches, wherein commodity loaches which grow quickly and are robust in size are selected from first filial generation 1-year old loaches one year ahead as thebreeding loaches, and breeding is strengthened for 0.5-3 months; 2, selection of the breeding loaches, wherein when the water temperature reaches 18 DEG C, various groups of female and male parent loaches are put in a parent loach grading sifting cylinder respectively to be sifted and matched; 3, artificial delivery acceleration; 4, artificial insemination; 5, after-delivery parent loach nursing.The parent loaches are effectively selected, synchronous sexual maturity of female and male loaches and the same batch of the parent loaches is improved, the delivery acceleration rate and the fry delivery tidiness rate are increased, and the artificial breeding efficiency is improved; meanwhile, the parent loach dead loss of artificial breeding is reduced.

The invention belongs to the agriculture technical field necessary for human life, and relates to a hybrid seedlings-cultivating method for improving the growth traits of the siniperca scherzeri. The method comprises the following main steps of: selecting the siniperca scherzeri as the male parent and the siniperca chuatsi as the female parent, and adopting the methods for building a plastic house to overwinter, intensively cultivate and the like; hastening the parturition by the means that the exogenous hormone is synchronously injected into the male parent and the female parent for once, and packing a parent fish by a dishtowel during artificial insemination; adopting the breeding technology and measurement such as the artificial semidry inseminating, the circuit water flowing hatching and the like, so that the use ratio of the male parent is improved by 1.5-2 times; and intensifying the cultivating management in the seedling stage and the cultivating stage by a segmented cultivating mode with the palatable bait fish. The invention successfully develops a method for improving the growth traits of the siniperca scherzeri to cultivate an improved new product which is antiretroviral, disease-resistant and faster in growth speed compared with the common siniperca scherzeri. Meanwhile, the existing hybrid seedlings-cultivating method of the siniperca scherzeri is changed, the synchronous mature of the parent is guaranteed, the propagation time is prolonged, the operation is simpler and more convenient, and the production efficiency and the survival rate of the artificial propagating and the hybrid seedlings hatching are improved.

The invention provides a hybrid carp and pengze crucian carp hybrid breeding method and belongs to the technical field of freshwater fish hybrid breeding. The hybrid carp and pengze crucian carp hybrid breeding method aims at solving the problem that no proper hybrid method exists in the prior art to prevent carp diseases in the northern area. The method includes the steps that a first filial generation is obtained by regarding a German mirror carp as a female parent and a jian carp as a male parent for hybridization, an irregular male carp with the high growth speed and a few large body surface scales serves as a male parent, a batch of plump female pengze crucian carps with more than 31 lateral line scales and high growth speed are optimized out through two-generation breeding to serve as a female parent, parent mature promotion and artificial propagation are carried out on the basis of optimizing and directive breeding of hybrid parents, hybrid carps coincident with the pengze crucian carp female parent in appearance can be obtained after distant hybridization is carried out, hybrid generation has no carp characters, and the hybrid carp and pengze crucian carp hybrid breeding method has the advantages of being high in growth speed and disease resistance, and is of great significance in improving unit yield, increasing breeding benefits, breeding new species and achieving large-scale fry rearing.

The invention discloses an artificial spawning induction method for Trochus pyramis born. The method comprises the steps that (1) Trochus pyramis born parent cowries were put in a breeding pool with culture of benthic diatom for maturity acceleration breeding, the cowries are put into normal-temperature seawater when the water temperature in the breeding pool reaches 25 DEG C or above, and the water temperature is increased gradually through sun exposure and continuous air inflation; and (2) the seawater exposed to sunlight is discharged and replaced by fresh filtered seawater, the water temperature is recovered to the normal temperature, air is inflated indoors and spawning waiting is conducted. The method disclosed by the invention is characterized in that natural temperature differences are utilized; sunlight exposure takes the place of dedicated ultraviolet ray generators and temperature rise devices; an ultraviolet ray induction method and a temperature variation induction method are integrated to induce sperm egg discharge of Trochus pyramis born. The spawning induction method disclosed by the invention has the advantages that the spawning induction rate reaches up to 89%; and the fertility rate reaches up to 100%. The method disclosed by the invention has the advantages that no influences are brought to creatures and environments; and the method is safe, reliable, efficiency and easy in operations.

The invention belongs to the technical field of aquaculture, and relates to an efficient artificial spawning induction method for cynoglossus semilaevis female fish. According to the method, efficientartificial spawning induction of cynoglossus semilaevis female parent fish is achieved through selection of the female parent fish, control over spawning induction pond culture conditions, artificialspawning induction, artificial insemination and incubation. By the adoption of the method, the spawning induction rate, the relative spawning amount, the fertilization rate and the hatching rate of the cynoglossus semilaevis female fish are greatly increased, and the method is an extremely efficient artificial spawning induction method. The method provided by the invention has very good practicability and operability, and is beneficial to further rapid development of the cynoglossus semilaevis culture industry.

The invention discloses an efficient spawning induction method for quasipaa spinosa. The method comprises the following steps: mixing spawning induction feed with water, and directly introducing the mixture into a quasipaa spinosa pond for feeding; and mixing the mixed hormone, the amino acid additive, the pectin and the centella asiatica extract for feeding. The discomfortableness of organisms caused by hormone feeding can be reduced, and meanwhile, the seaweed meal is added; polysaccharide substances in the seaweed meal and pectin are used together, so that when the seaweed meal is dissolved in water, mixed hormones and nutrient substances separated out of the feed can be gelatinized, and the effect is better; meanwhile, the freshness and the effective duration of mixed hormones and separated nutrient substances can be prolonged; the method also includes feeding with mixed hormones and simulating the temperature and environment in the estrus period, so as to unconsciously influence the estrus condition of the frogs to be induced to spawn; a method of directly injecting oxytocic hormone is combined, so that the oxytocic rate and the individual spawning rate are increased; meanwhile, the injury of spawning induction to the quasipaa spinosa is reduced.

The invention provides a method for artificial breeding of wild Schizothorax Lhasa, comprising the following steps: A, broodstock collection: collect Lhasa Schizothorax broodstock in the Yarlung Zangbo River from early April to late May each year; B, artificially induced labor: before induced labor, First anesthetize the parent fish with an anesthetic, and then give the female fish and male fish a stimulant injection; C. Artificial insemination: After the parent fish is anesthetized with an anesthetic, collect semen and eggs, and use dry insemination; D. Fertilized eggs hatch: sarcomeres appear Before the period, place the fertilized eggs in the hatching frame for hatching in running water; after the fertilized eggs develop to the sarcomere stage, remove the dead eggs, and move the fertilized eggs into the fish egg incubator to hatch; E, fry open. Realize large-scale artificial breeding of Lhasa Schizothorax.

The invention discloses a method for artificial propagation of copperfish. The steps are: (1) Broodstock selection: According to the external characteristics of the broodstock and the eggs dug out by the egg digger, select the broodstock with high maturity of gonads as the broodstock for induced labor; (2) Preparation and injection of oxytocin drugs: Body weight using normal saline to prepare the oxytocic drug luteinizing hormone releasing hormone A 3 , fish pituitary gland and chorionic gonadotropin; the injection adopts the double injection method, the water temperature is 20.5~22.0°C, and the needle distance is 8~10 h; (3) Artificial induced labor and insemination: After injecting the oxytocin drug, check the broodstock from time to time, Ovulation and fertilization are found, and artificial insemination is carried out. The insemination method is wet fertilization; (4) Incubation: the obtained fertilized eggs are poured into a conical incubator for incubation, and the fry are moved to the fry incubation box for seed cultivation after they emerge from the film. The operation is simple, and the artificial reproduction rate and fry survival rate (above 85%) are effectively improved, laying a solid foundation for the proliferation and release of copperfish population resources.

The invention provides a method for artificially induced spawning of ovate pompano, comprising: (1) selecting broodstock, (2) intensively cultivating the broodstock to induce spawning, increasing the water temperature by 0.3-0.5°C every 2-3 days; 6-10% of the pool water was replaced with compound seawater every day; the enhanced bait was fed 3-4 times a day; the light was extended for 10-15 minutes every two days; (3) oxytocic agent was injected with temperature control for 3 times to artificially induce labor; After the temperature-controlled injection of oxytocic agent for the first time, adjust the water flow rate to 0.8-1.4m3 / s, and extend the photoperiod to 15L / 9D-16L / 8D; (4) Artificial insemination and hatching. In the present invention, the broodstock is induced to be induced by intensive breeding, and three temperature-controlled injections are used to induce the broodstock to induce spawning under different water temperature environments, and the water flow rate and the light cycle are increased after the injection of the oxytocic agent, so as to effectively improve the broodstock induced spawning effect, and The effect time is shortened, and the fertilization rate and hatching rate are high.

The invention discloses a low-loss and high-efficiency artificial propagation method for Taiwan loach, which is characterized in that it comprises the following steps: (1) Prenatal cultivation of species of loach: selecting fast-growing and robust individuals from the first-year-old fish of the offspring one year in advance The commercial loach is used as a seed loach, and it is intensively cultivated for 0.5-3 months. (2) The selection of the seed loach: the water temperature reaches 18 degrees Celsius, and each group of male and female loach is placed in the parent loach classification screening tank for screening and matching; (3) Artificial induced labor ; (4) artificial insemination; (5) postpartum pro-loach care. Effectively select brood loach, improve the simultaneous sexual maturation of male and female and the same batch of brood loach, increase the induced labor rate, emergence rate and artificial reproduction efficiency, and reduce the death loss of brood loach in artificial breeding.